LOCUS       sequence-430546-        7079 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  NBU2 backbone with anti-RbsR(YQR) and P(O1)_nanoluc.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7079)
  AUTHORS   Huang BD, Groseclose TM, Wilson CJ
  TITLE     Transcriptional programming in a Bacteroides consortium.
  JOURNAL   Nat Commun. 2022 Jul 6;13(1):3901. doi: 10.1038/s41467-022-31614-8.
  PUBMED    35794179
REFERENCE   2  (bases 1 to 7079)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-07-6"; volume: "13(1):3901. doi"; pages: " 
            10.1038/s41467-022-31614-8"
COMMENT     Sequence Label: pBH116
FEATURES             Location/Qualifiers
     source          1..7079
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    177..193
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    210..226
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(1270..1289)
                     /label=LacI-R
                     /note="LacI, reverse primer"
     CDS             join(5523..5591,5592..6383)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5741..5760)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     oriT            6514..6623
                     /note="incP origin of transfer"
     rep_origin      join(6794..7079,1..103)
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
ORIGIN
        1 gtttaacggt tgtggacaac aagccaggga tgtaacgcac tgagaagccc ttagagcctc
       61 tcaaagcaat tttgagtgac acaggaacac ttaacggctg acatgggaat tcccctccac
      121 cgcggtggta caaagaaaat tcgacaaact gttatttttc tatctattta tttgaattgt
      181 gagcggataa caattacctt tgtcggcaat tgtgagcgga taacaattaa ataaagatat
      241 tctcgtcaaa caaatataaa taatataaac atggttttta ctctggaaga ttttgttggc
      301 gattggcgtc agaccgcggg ttataatttg gatcaagtcc tggaacaggg tggcgtaagc
      361 tctctgttcc agaacctggg tgtgagcgtg acgccgattc agcgcatcgt tctgtccggc
      421 gagaacggtc tgaaaattga tattcatgtg atcatcccgt acgaaggcct gagcggtgac
      481 caaatgggtc aaatcgagaa aatctttaaa gtcgtctacc cagttgacga tcaccacttc
      541 aaggttatct tgcattacgg tacgctggtg attgatggtg tgaccccgaa tatgattgac
      601 tatttcggcc gtccgtatga aggcattgcc gtttttgacg gtaaaaagat caccgtcacc
      661 ggtaccctgt ggaatggcaa taagattatt gacgagcgtc tgattaaccc ggacggcagc
      721 ctgctgttcc gcgtgaccat caacggtgtc acgggttggc gtctgtgcga gcgcatcctg
      781 gcataatgaa ctgcacttgc tttgataatt aatgataaac aatctaaaag cactctaatc
      841 gttatcggag tgcttttaga ttactaatca aattgcttct actaattgcc tatcttccag
      901 tgatggaaca gcatttgtgc attggctgca acaatcagcc ttgatctgga agaagcaatg
      961 aaagctgctg ttaagtctcc gaatcaggta ttgttcctga caggtgtatt cccatccggt
     1021 aaacgcggat actttgcagt tgatctgact caggaataaa ttataaatta aggtaagaag
     1081 attgtaggat aagctaatga aatagaaaaa ggatgccgtc acacaacttg tcggcattct
     1141 tttttgtttt attagttgaa aatatagtga aaaagttgcc taaatatgta tgttaacaaa
     1201 ttatttgtcg taactttgca ctccaaatct gtttttaaca tatggcacta gtgaaaccag
     1261 taacgttata cgatgtcgca gagtatgccg gtgtctctta tcagaccgtt tcccgcgtgg
     1321 tgaaccaggc cagccacgtt tctgcgaaaa cgcgggaaaa agtggaagcg gcgatggcgg
     1381 agctcaatta cattcccaac cgcgtggcac aacaactggc gggcaaagcg tcgcatacca
     1441 ttggcatgtt gatcactgcc agtaccaatc ctttctattc agaactggtg cgtggcgttg
     1501 aacgcagctg cttcgaacgc ggttatagtc tcgatctttg caataccgaa ggcgatgaac
     1561 agcggatgaa tcgcaatctg gaaacgctga tgcaaaaacg cgttgatggc ttgctgttac
     1621 tgtgcaccga aacgcatcaa ccttcgcgtg aaatcatgca acgttatccg acagtgccta
     1681 ctgtgatgat ggactgggct ccgttcgatg gcgacagcga tcttattcag gataactcgt
     1741 tgctgggcgg agacttagca acgcaatatc tgatcgataa aggtcatacc cgtatcgcct
     1801 gtattaccgg cccgctggat aaaactccgg cgcgcctgcg gttggaaggt tatcgggcgg
     1861 cgatgaaacg tgcgggtctc aacattcctg atggctatga agtcactggt gattttgaat
     1921 ttaacggcgg gtttgacgct atgcgccaac tgctatcaca tccgctgcgt cctcaggccg
     1981 tctttaccgg aaatgacgct atggctgttg gcgtttacca ggcgttatat caggcagagt
     2041 tacaggttcc gcaggatatc gcggtgattg gctatgacga tatcgaactg gcaagcttta
     2101 tgacgccacc attaaccact atccaccaac cgaaagatga actgggggag ctggcgattg
     2161 atgtactcat ccatcggata acccagccga cccttcagca acaacgatta caacttactc
     2221 cgattctgat ggaacgcggt tcggcttagc tggtgaaaag aaaaaccacc ctggcgccca
     2281 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg
     2341 tttcccgact ggaaagcggg cagtgagctt tcctcggtac caaattccag aaaagaggcc
     2401 tcccgaaagg ggggcctttt ttcgttttgg tcctacttgt gcctgttcta tttccgaacc
     2461 gaccgcttgt atgaatgcat caaaattcgt tttctctacg ttggattcct tgttgctcat
     2521 attgtgatga taatttctac aaatatagtc attggtaact atctatgaaa ctgtttgata
     2581 cttttatagt tgattaaact tgttcatggc atttgcctta atatcatccg ctatgtcaat
     2641 gtagggtttc atagctttgt agtcgctgtg tcccgtccat ttcatgacca cctgtgccgg
     2701 gattccgaga gccagcgcat tgcagatgaa tgtccttttt cctgcatggg tactgagcaa
     2761 agcgtatttg ggtgtgactt catcaatacg ttcatttccc ttgtagtagg tttcccgtac
     2821 aggctcgttg atttctgcca gttcacccag ctctttcagg taatcgttca tcttctggtt
     2881 gctgatgacg ggcagagcca tgtaattctc gaaatggatg tccttgtatt tgtccagtat
     2941 ggctttgctg tatttgttca gttcaatcgt caggctgtcg gcagtcttga ctgtggttat
     3001 ttcgatgtgg tcggacttca catcgcttct tttcagattg cgaacatccg aataccgcaa
     3061 actcgtaaag cagcagaaca ggaaaacatc acgcacacgt tccaggtatt gcttatcctt
     3121 gggtatctgg tagtctttca gcttgttcag ttcatcccaa gtcaggaaga ttactttttt
     3181 cgaggtggtt ttcagtttcg gtttgaacgt atcgtatgca atgttctgat gatgtccttt
     3241 cttgaagctc cagcgcagga accatttgag gaatcccatt tgcttgccga tggtgctgtt
     3301 tctcatatcc ttggtgtcac gcaggaagtt gacgtattcg ttcaatccaa actcgttgaa
     3361 atagttgaac gttgcatcct ccttgaactc tttgaggtgg ttcctcactg ctgcaaattt
     3421 ttcataggtg gatgccgtcc agttattctg gttaccgcac tcttttacaa actcatcgaa
     3481 cacctcccaa aagctgacag gggcttcttc cggctgttct tcactggtgt ctttcattct
     3541 catgttgaaa gcttccttca actgttgggt cgttggcatg acctcctgca cctcaaattc
     3601 cttgaaaata ttctggattt cggcatagta tttcagcaag tccgtattga tttcggctgc
     3661 actttgcttt agcttgttgg tacatccgtt ctttacccgc tgcttatcta catcccattt
     3721 ggctacgtca atccggtagc ccgttgtaaa ctcgatacgt tggctggcaa agatgacacg
     3781 catacggatg ggtacgttct ctacgattgg cacaccgttc tttttccggc tctccaatgc
     3841 aaaaatgatg ttgcgcttga tattcataat tgggtgcgtt tgaaattcta cacccaaata
     3901 tacacccaat tattgagata gcaaaagaca tttagaaaca tttactttta ctctatattg
     3961 taatttacac ttgattatca gtcgtttgca gttttatgat attctgtgaa agtataagtt
     4021 cgagagcctg tctctccgca aaaaacgctg aaaatcagca gattgcaaaa caaacaccct
     4081 gttttacacc caagaatgta aagtcgggtg tttttgtttt atttaagata atacaaccac
     4141 tacataataa aagagtagcg atattaaaag aatccgatga gaaaagacta atatttatct
     4201 atccattcag tttgatttct caggacttta catcgtcctg aaagtatttg ttggtaccga
     4261 ggacgcgtaa acatttacag ttgcatgtgg cctattgttt ttagccgtta aatattttat
     4321 aactattaaa tagcgataca aattgttcga aactaatatt gtttatatca tatattctcg
     4381 catgttttaa agctttatta aattgatttt ttgtaaacag tttttcgtac tctttgttaa
     4441 cccatttcat tacaaaagtt tcatattttt ttctctcttt aaatgccatt tttgctggct
     4501 ttctttttaa tacaattaat gtgctatcca ctttaggttt tggatggaaa taatacctag
     4561 gaatttttgc taatatagaa atatctacct ctgccattaa cagcaatgct agtgatctgt
     4621 ttgtatctaa taacatttta gcaaaaccat attccactat taaataactt attgtggctg
     4681 aactttcaaa aacaattttt cgaattatat ttgtgcttat gttgtaaggt atgctgccaa
     4741 atattttata tggattgtgg ctaggaaatg taaatttcag tatatcatca tttactattt
     4801 gatagttagg ataatttaag agcttattac gagttacctc acataattta gaatcaattt
     4861 ctatcgccgt tacaaaatta catctcttta ccaatccagc agtaaaatga cctttccctg
     4921 cacctatttc aaagatgtta tctttttcat ctaaacttat gcaattcatt attttttcta
     4981 tgtgatattt tgaagtaata aaattttgac tatcttttat atttactttg ttcattataa
     5041 cctctcctta atttattgca tctcttttcg aatatttatg ttttttgaga aaagaacgta
     5101 ctcatggttc atcccgatat gcgtatcggt ctgtatatca gcaactttct atgtgtttca
     5161 actacaatag tcatctattc tcatctttct gagtccaccc cctgcaaagc ccctctttac
     5221 gacataaaaa ttcggtcgga aaaggtatgc aaaagatgtt tctctcttta agagaaactc
     5281 ttcgggatgc aaaaatatga aaataactcc aattcaccaa attatatagc gactttttta
     5341 caaaatgcta aaatttgttg atttccgtca agcaattgtt gagcaaaaat gtcttttacg
     5401 ataaaatgat acctcaatat caactgttta gcaaaacgat atttctctta aagagagaaa
     5461 cacctttttg ttcaccaatc cccgactttt aatcccgcgg ccatgattga aaaaggaaga
     5521 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     5581 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     5641 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     5701 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     5761 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     5821 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     5881 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     5941 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     6001 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     6061 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     6121 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     6181 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
     6241 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     6301 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     6361 cctcactgat taagcattgg taactgtcag accaagttta ctcataacgc gtcaattcga
     6421 gggggatcaa ttccgtgata ggtgggctgc ccttcctggt tggcttggtt tcatcagcca
     6481 tccgcttgcc ctcatctgtt acgccggcgg tagccggcca gcctcgcaga gcaggattcc
     6541 cgttgagcac cgccaggtgc gaataaggga cagtgaagaa ggaacacccg ctcgcgggtg
     6601 ggcctacttc acctatcctg cccggctgac gccgttggat acaccaagga aagtctacac
     6661 gaaccctttg gcaaaatcct gtatatcgtg cgaaaaagga tggatatacc gaaaaaatcg
     6721 ctataatgac cccgaagcag ggttatgcag cggaaaacgg aattgatccg gccacgatgc
     6781 gtccggcgta gaggatctga agatcagcag ttcaacctgt tgatagtacg tactaagctc
     6841 tcatgtttca cgtactaagc tctcatgttt aacgtactaa gctctcatgt ttaacgaact
     6901 aaaccctcat ggctaacgta ctaagctctc atggctaacg tactaagctc tcatgtttca
     6961 cgtactaagc tctcatgttt gaacaataaa attaatataa atcagcaact taaatagcct
     7021 ctaaggtttt aagttttata agaaaaaaaa gaatatataa ggcttttaaa gcttttaag
//
