LOCUS       sequence-430548-       11189 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  NBU1 backbone with constitutive dCas9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11189)
  AUTHORS   Huang BD, Groseclose TM, Wilson CJ
  TITLE     Transcriptional programming in a Bacteroides consortium.
  JOURNAL   Nat Commun. 2022 Jul 6;13(1):3901. doi: 10.1038/s41467-022-31614-8.
  PUBMED    35794179
REFERENCE   2  (bases 1 to 11189)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-07-6"; volume: "13(1):3901. doi"; pages: " 
            10.1038/s41467-022-31614-8"
COMMENT     Sequence Label: pBH501
FEATURES             Location/Qualifiers
     source          1..11189
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(460..486,487..489,490..2976,2977..2979,2980..4566)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGEFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             join(9605..9673,9674..10465)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9823..9842)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     oriT            10596..10705
                     /note="incP origin of transfer"
     rep_origin      join(10876..11189,1..75)
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
ORIGIN
        1 gatgtaacgc actgagaagc ccttagagcc tctcaaagca attttgagtg acacaggaac
       61 acttaacggc tgacatggga attcccctcc accgcggtgt attaatgcgg ctgcccctgc
      121 acctgggtag ctcaatgcgc gcaataaaaa agcccccgga aggtgatctt ccgggggctt
      181 tctcatgcgt tggtgttcac tcccattcta tgcggttcct ggccttttgc tggccttttg
      241 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
      301 agtgagctga tacatcccaa atgctgctcg gtaccaaaga cgaacaataa gacgctgaaa
      361 agcgtctttt ttcgttttgg tccgataaag tttggaagat aaagctaaaa gttcttatct
      421 ttgcagtccg aaataaagac atataaaaga aaagacacca tggataagaa atactcaata
      481 ggcttagcta tcggcacaaa tagcgtcgga tgggcggtga tcactgatga atataaggtt
      541 ccgtctaaaa agttcaaggt tctgggaaat acagaccgcc acagtatcaa aaaaaatctt
      601 ataggggctc ttttatttga cagtggagag acagcggaag cgactcgtct taaacggaca
      661 gctcgtagaa ggtatacacg tcggaagaat cgtatttgtt atctacagga gattttttca
      721 aatgagatgg cgaaagtaga tgatagtttc tttcatcgac ttgaagagtc ttttttggtg
      781 gaagaagaca agaagcatga acgtcatcct atttttggaa atatagtaga tgaagttgct
      841 tatcatgaga aatatccaac tatctatcat ctgcgaaaaa aattggtaga ttctactgat
      901 aaagcggatt tgcgcttaat ctatttggcc ttagcgcata tgattaagtt tcgtggtcat
      961 tttttgattg agggagattt aaatcctgat aatagtgatg tggacaaact atttatccag
     1021 ttggtacaaa cctacaatca attatttgaa gaaaacccta ttaacgcaag tggagtagat
     1081 gctaaagcga ttctttctgc acgattgagt aaatcaagac gattagaaaa tctcattgct
     1141 cagctccccg gtgagaagaa aaatggctta tttgggaatc tcattgcttt gtcattgggt
     1201 ttgaccccta attttaaatc aaattttgat ttggcagaag atgctaaatt acagctttca
     1261 aaagatactt acgatgatga tttagataat ttattggcgc aaattggaga tcaatatgct
     1321 gatttgtttt tggcagctaa gaatttatca gatgctattt tactttcaga tatcctaaga
     1381 gtaaatactg aaataactaa ggctccccta tcagcttcaa tgattaaacg ctacgatgaa
     1441 catcatcaag acttgactct tttaaaagct ttagttcgac aacaacttcc agaaaagtat
     1501 aaagaaatct tttttgatca atcaaaaaac ggatatgcag gttatattga tgggggagct
     1561 agccaagaag aattttataa atttatcaaa ccaattttag aaaaaatgga tggtactgag
     1621 gaattattgg tgaaactaaa tcgtgaagat ttgctgcgca agcaacggac ctttgacaac
     1681 ggctctattc cccatcaaat tcacttgggt gagctgcatg ctattttgag aagacaagaa
     1741 gacttttatc catttttaaa agacaatcgt gagaagattg aaaaaatctt gacttttcga
     1801 attccttatt atgttggtcc attggcgcgt ggcaatagtc gttttgcatg gatgactcgg
     1861 aagtctgaag aaacaattac cccatggaat tttgaagaag ttgtcgataa aggtgcttca
     1921 gctcaatcat ttattgaacg catgacaaac tttgataaaa atcttccaaa tgaaaaagta
     1981 ctaccaaaac atagtttgct ttatgagtat tttacggttt ataacgaatt gacaaaggtc
     2041 aaatatgtta ctgaaggaat gcgaaaacca gcatttcttt caggtgaaca gaagaaagcc
     2101 attgttgatt tactcttcaa aacaaatcga aaagtaaccg ttaagcaatt aaaagaagat
     2161 tatttcaaaa aaatagaatg ttttgatagt gttgaaattt caggagttga agatagattt
     2221 aatgcttcat taggtaccta ccatgatttg ctaaaaatta ttaaagataa agattttttg
     2281 gataatgaag aaaatgaaga tatcttagag gatattgttt taacattgac cttatttgaa
     2341 gatagggaga tgattgagga aagacttaaa acatatgctc acctctttga tgataaggtg
     2401 atgaaacagc ttaaacgtcg ccgttatact ggttggggac gtttgtctcg aaaattgatt
     2461 aatggtatta gggataagca atctggcaaa acaatattag attttttgaa atcagatggt
     2521 tttgccaatc gcaattttat gcagctgatc catgatgata gtttgacatt taaagaagac
     2581 attcaaaaag cacaagtgtc tggacaaggc gatagtttac atgaacatat tgcaaattta
     2641 gctggtagcc ctgctattaa aaaaggtatt ttacagactg taaaagttgt tgatgaattg
     2701 gtcaaagtaa tggggcggca taagccagaa aatatcgtta ttgaaatggc acgtgaaaat
     2761 cagacaactc aaaagggcca gaaaaattcg cgagagcgta tgaaacgaat cgaagaaggt
     2821 atcaaagaat taggaagtca gattcttaaa gagcatcctg ttgaaaatac tcaattgcaa
     2881 aatgaaaagc tctatctcta ttatctccaa aatggaagag acatgtatgt ggaccaagaa
     2941 ttagatatta atcgtttaag tgattatgat gtcgatgcca ttgttccaca aagtttcctt
     3001 aaagacgatt caatagacaa taaggtctta acgcgttctg ataaaaatcg tggtaaatcg
     3061 gataacgttc caagtgaaga agtagtcaaa aagatgaaaa actattggag acaacttcta
     3121 aacgccaagt taatcactca acgtaagttt gataatttaa cgaaagctga acgtggtggt
     3181 ttgagtgaac ttgataaagc tggttttatc aaacgccaat tggttgaaac tcgccaaatc
     3241 actaagcatg tggcacaaat tttggatagt cgcatgaata ctaaatacga tgaaaatgat
     3301 aaacttattc gagaggttaa agtgattacc ttaaaatcta aattagtttc tgacttccga
     3361 aaagatttcc aattctataa agtacgtgag attaacaatt accatcatgc ccatgatgcg
     3421 tatctaaatg ccgtcgttgg aactgctttg attaagaaat atccaaaact tgaatcggag
     3481 tttgtctatg gtgattataa agtttatgat gttcgtaaaa tgattgctaa gtctgagcaa
     3541 gaaataggca aagcaaccgc aaaatatttc ttttactcta atatcatgaa cttcttcaaa
     3601 acagaaatta cacttgcaaa tggagagatt cgcaaacgcc ctctaatcga aactaatggg
     3661 gaaactggag aaattgtctg ggataaaggg cgagattttg ccacagtgcg caaagtattg
     3721 tccatgcccc aagtcaatat tgtcaagaaa acagaagtac agacaggcga attctccaag
     3781 gagtcaattt taccaaaaag aaattcggac aagcttattg ctcgtaaaaa agactgggat
     3841 ccaaaaaaat atggtggttt tgatagtcca acggtagctt attcagtcct agtggttgct
     3901 aaggtggaaa aagggaaatc gaagaagtta aaatccgtta aagagttact agggatcaca
     3961 attatggaaa gaagttcctt tgaaaaaaat ccgattgact ttttagaagc taaaggatat
     4021 aaggaagtta aaaaagactt aatcattaaa ctacctaaat atagtctttt tgagttagaa
     4081 aacggtcgta aacggatgct ggctagtgcc ggagaattac aaaaaggaaa tgagctggct
     4141 ctgccaagca aatatgtgaa ttttttatat ttagctagtc attatgaaaa gttgaagggt
     4201 agtccagaag ataacgaaca aaaacaattg tttgtggagc agcataagca ttatttagat
     4261 gagattattg agcaaatcag tgaattttct aagcgtgtta ttttagcaga tgccaattta
     4321 gataaagttc ttagtgcata taacaaacat agagacaaac caatacgtga acaagcagaa
     4381 aatattattc atttatttac gttgacgaat cttggagctc ccgctgcttt taaatatttt
     4441 gatacaacaa ttgatcgtaa acgatatacg tctacaaaag aagttttaga tgccactctt
     4501 atccatcaat ccatcactgg tctttatgaa acacgcattg atttgagtca gctaggaggt
     4561 gactgattct ttgtacccga taattgccta tcttccagtg atggaacagc atttgtgcat
     4621 tggctgcaac aatcagcctt acttgtgcct gttctatttc cgaaccgacc gcttgtatga
     4681 atgcatcaaa attcgttttc tctacgttgg attccttgtt gctcatattg tgatgataat
     4741 ttctacaaat atagtcattg gtaactatct atgaaactgt ttgatacttt tatcagtcca
     4801 gtagttttac aaggtctttc ttcatttctt cgtcaatatc cctgtatcgt ctgaaagctt
     4861 tgcttccctc cttgtgtccc gacagtgcgg aaacaaggtt cgggtctttc acttttttat
     4921 agatattgcc gataaacgta cgtcttgcca gatggctgct tgccacttca taaataggtc
     4981 gtttgatttc gttgtgcgtc aacgggtcta agattgttac gatgcggtca actccagcta
     5041 atttgaatat cttttttatg gcatcattgt acttttgctc ggatatgaac ggcaacagtt
     5101 ttccctcata ttctttgtag cgttcaagga tttctttcgc tttgtcgtta agtggaacac
     5161 gtaccgtaac cggattcccc tctttggttt tcttgggaat atattctatg gcttcattga
     5221 ccacatttag tttggtcatt cggtacaggt cgctcaccct gcatcctatc agtgtctgaa
     5281 atatgaatat atccctctgt attgccagtt gtggggtggc agaaaggtct gcattaaaaa
     5341 tcctgtccct ttcttcgagt gttatataat aaggtgtacc atatgtacac tcctctatcg
     5401 gaaacttgtc gaaaggtctg tttgtggtgc gtttgttatc gaagcaccac aggaagaatg
     5461 tgcgtattct tgaaaaacag tctatcagcg tgtttttgct tctgggctgt ggtgtcctct
     5521 tttcgggaat ggcttcataa atgctcgggt aaagttcata atactggtat tcgttctgaa
     5581 agaaatccca catatcccga agcgtgtcag gtgttaccaa atccacatca aggataaagc
     5641 ccttttgtcc tctctttgta gcccttacat atagttcata acgcagtaag gctcttttga
     5701 caacccggaa atttttcttt cgtacttccg acaaagggtg cttgtttaga aattcatcga
     5761 atagttctcc aatggtaggc ttgataacca cttcctccgg caaaaaatat ttttcaggat
     5821 ggtaaaattt atcaagtgtt gtttttagcc attctttgtc tattgcttcc ttttcttgtt
     5881 gatatacctt ctcgatatag gttttcaact ggcgtatctc ttcatttata tgggtacgca
     5941 tttcttcatt gcagacagct ttcgttttta cacattcgtc tttatcatcc catagattgg
     6001 gattgatggc taactgagta ggagcaacgg agtctaactg ccttccgttt ctaaaacgga
     6061 catagattgt agccatggat tctgtatcat atcgtttggc tgctttttta atgataaagg
     6121 ttactttcat agactttcag gttgaatttt actctgctgc aaatataaat attttcccca
     6181 gcattttccc cacatctgct aaatattttg caattcgatt aaacttggat taaaatttaa
     6241 taggattata acatattgaa atacagtgta attgtggcgt ttttccgcat ttttcttttt
     6301 acccacattt tccccacttt aattatttca aatgcggggt ctgggtacaa gaaagaaagc
     6361 taagtatttg atagttcaat acttagcttt ttcttttgct tgaattttcc ccacattttc
     6421 cccacacgtg caaaaaatat agcagtaagt cattatttct tttggttgaa cgtagagagt
     6481 agcgatatta aaagaatccg atgagaaaag actaatattt atctatccat tcagtttgat
     6541 ttctcaggac tttacatcgt cctgaaagta tttgttggta ccgaggacgc gtaaacattt
     6601 acagttgcat gtggcctatt gtttaggacg cgttatctcc ttaacgtacg ttttcgttcc
     6661 attggccctc aaaccccgtt atatacattc atgtccattt atgtaaaaaa tcctgctgac
     6721 cttgtttatg tcttgtcagt caccatttgc aaaaccatat ttgaccctca aagaggctga
     6781 atttgataag caacttgcta catactcata ataaggagct aaatagaaca cgaatgggaa
     6841 atactcaaat gccaaactaa agaagatatt ggccaaaata aacgctatac cgagagagaa
     6901 acttgatttt tcaacttcct aaaacagtgt tgttcaaaca tttctactta tttgtactta
     6961 ccagttgaac ctacgtttcc ctaataaaat gtctatggta aaaagttaaa aaatcctcct
     7021 acttttgtta gatatatttt tttgtgtaat tttgtaatcg ttatgcggca gtaataatat
     7081 acatattaat acgagttagg aatcctgtag ttctcatatg ctacgaggag gtattaaaag
     7141 gtgcgtttcg acaatgcatc tattgtagta tattattgct taatccaaat gaatattata
     7201 aatttaggaa ttcttgctca cattgatgca ggaaaaactt ccgtaaccga gaatctgctg
     7261 tttgccagtg gagcaacgga aaagtgcggc tgtgtggata atggtgacac cataacggac
     7321 tctatggata tagagaaacg tagaggaatt actgttcggg cttctacgac atctattatc
     7381 tggaatggtg tgaaatgcaa tatcattgac actccgggac acatggattt tattgcggaa
     7441 gtggagcgga cattcaaaat gcttgatgga gcagtcctca tcttatccgc aaaggaaggc
     7501 atacaagcgc agacaaagtt gctgttcaat actttacaga agctgcaaat cccgacaatt
     7561 atatttatca ataagattga ccgagccggt gtgaatttgg agcgtttgta tctggatata
     7621 aaagcaaatc tgtctcaaga tgtcctgttt atgcaaaatg ttgtcgatgg atcggtttat
     7681 ccggtttgct cccaaacata tataaaggaa gaatacaaag aatttgtatg caaccatgac
     7741 gacaatatat tagaacgata tttggcggat agcgaaattt caccggctga ttattggaat
     7801 acgataatcg ctcttgtggc aaaagccaaa gtctatccgg tgctacatgg atcagcaatg
     7861 ttcaatatcg gtatcaatga gttgttggac gccatcactt cttttatact tcctccggca
     7921 tcggtctcaa acagactttc atcttatctt tataagatag agcatgaccc caaaggacat
     7981 aaaagaagtt ttctaaaaat aattgacgga agtctgagac ttcgtgacgt tgtaagaatc
     8041 aacgattcgg aaaaattcat caagattaaa aatctaaaaa ctatcaatca gggcagagag
     8101 ataaatgttg atgaagtggg cgccaatgat atcgcgattg tagaggatat ggatgatttt
     8161 cgaatcggaa attatttagg tgctgaacct tgtttgattc aaggattatc gcatcagcat
     8221 cccgctctca aatcctccgt ccggccagac aggcccgaag agagaagcaa ggtgatatcc
     8281 gctctgaata cattgtggat tgaagacccg tctttgtcct tttccataaa ctcatatagt
     8341 gatgaattgg aaatctcgtt atatggttta acccaaaagg aaatcataca gacattgctg
     8401 gaagaacgat tttccgtaaa ggtccatttt gatgagatca agactatata caaagaacga
     8461 cctgtaaaaa aggtcaataa gattattcag atcgaagtgc cgcccaaccc ttattgggcc
     8521 acaatagggc tgactcttga acccttaccg ttagggacag ggttgcaaat cgaaagtgac
     8581 atctcctatg gttatctgaa ccattctttt caaaatgccg tttttgaagg gattcgtatg
     8641 tcttgccaat ccgggttaca tggatgggaa gtgactgatc tgaaagtaac ttttactcaa
     8701 gccgagtatt atagcccggt aagtacacct gctgatttca gacagctgac cccttatgtc
     8761 ttcaggctgg ccttgcaaca gtcaggtgtg gacattctcg aaccgatgct ctattttgag
     8821 ttgcagatac cccaagcggc aagttccaaa gctattacag atttgcaaaa aatgatgtct
     8881 gagattgaag acatcagttg caataatgag tggtgtcata ttaaagggaa agttccatta
     8941 aatacaagta aagactatgc atcagaagta agttcataca ctaagggctt aggcattttt
     9001 atggttaagc catgcgggta tcaaataaca aaaggcggtt attctgataa tatccgcatg
     9061 aacgaaaaag ataaactttt attcatgttc caaaaatcaa tgtcatcaaa ataatggtat
     9121 aacctctcct taatttattg catctctttt cgaatattta tgttttttga gaaaagaacg
     9181 tactcatggt tcatcccgat atgcgtatcg gtctgtatat cagcaacttt ctatgtgttt
     9241 caactacaat agtcatctat tctcatcttt ctgagtccac cccctgcaaa gcccctcttt
     9301 acgacataaa aattcggtcg gaaaaggtat gcaaaagatg tttctctctt taagagaaac
     9361 tcttcgggat gcaaaaatat gaaaataact ccaattcacc aaattatata gcgacttttt
     9421 tacaaaatgc taaaatttgt tgatttccgt caagcaattg ttgagcaaaa atgtctttta
     9481 cgataaaatg atacctcaat atcaactgtt tagcaaaacg atatttctct taaagagaga
     9541 aacacctttt tgttcaccaa tccccgactt ttaatcccgc ggccatgatt gaaaaaggaa
     9601 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     9661 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     9721 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     9781 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     9841 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     9901 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     9961 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
    10021 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
    10081 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
    10141 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
    10201 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
    10261 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
    10321 gtcacgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
    10381 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
    10441 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcataac gcgtcaattc
    10501 gagggggatc aattccgtga taggtgggct gcccttcctg gttggcttgg tttcatcagc
    10561 catccgcttg ccctcatctg ttacgccggc ggtagccggc cagcctcgca gagcaggatt
    10621 cccgttgagc accgccaggt gcgaataagg gacagtgaag aaggaacacc cgctcgcggg
    10681 tgggcctact tcacctatcc tgcccggctg acgccgttgg atacaccaag gaaagtctac
    10741 acgaaccctt tggcaaaatc ctgtatatcg tgcgaaaaag gatggatata ccgaaaaaat
    10801 cgctataatg accccgaagc agggttatgc agcggaaaac ggaattgatc cggccacgat
    10861 gcgtccggcg tagaggatct gaagatcagc agttcaacct gttgatagta cgtactaagc
    10921 tctcatgttt cacgtactaa gctctcatgt ttaacgtact aagctctcat gtttaacgaa
    10981 ctaaaccctc atggctaacg tactaagctc tcatggctaa cgtactaagc tctcatgttt
    11041 cacgtactaa gctctcatgt ttgaacaata aaattaatat aaatcagcaa cttaaatagc
    11101 ctctaaggtt ttaagtttta taagaaaaaa aagaatatat aaggctttta aagcttttaa
    11161 ggtttaacgg ttgtggacaa caagccagg
//
