LOCUS       sequence-43058-e        9276 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9276)
  AUTHORS   de Felippes FF, Wang JW, Weigel D
  TITLE     MIGS: MiRNA Induced Gene Silencing.
  JOURNAL   Plant J. 2011 Dec 28. doi: 10.1111/j.1365-313X.2011.04896.x.
  PUBMED    22211571
REFERENCE   2  (bases 1 to 9276)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J. 
            2011 Dec 28. doi: 10.1111/j.1365-313X.2011.04896.x."
COMMENT     Sequence Label: MIGS3.1 (FF537)
FEATURES             Location/Qualifiers
     source          1..9276
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    542..564
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA 
                     (truncated)"
     terminator      complement(588..840)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(860..1411)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(1453..1632)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     primer_bind     1869..1891
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1883..1900
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1884..1900
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1910..1929
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1910..1928
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     1953..1969
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     1954..1970
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2000..2018
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     2000..2017
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     terminator      complement(2051..2758)
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     promoter        4158..4503
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     4446..4466
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     protein_bind    4528..4652
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(4677..4682,4683..4700,4701..4707)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             4761..5420
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(4827..4846)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             5762..6067
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     5946..5965
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(6108..6232)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      6239..6880
                     /label=E9 terminator
                     /note="terminator and polyadenylation signal from the pea 
                     rbcS-E9 gene"
     promoter        complement(6902..6920)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6941..6957)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6941..6957)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6954..6976)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6965..6981
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6989..6995,6996..7014,7015..7020))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7035..7056
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7173..7190)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    7295..7319
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      complement(7410..7998)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7490..7509)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(8169..8957)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      join(9248..9276,1..407)
                     /label=pSa ori
                     /note="origin of replication from bacterial plasmid pSa"
ORIGIN
        1 tttttatccc cggaagcctg tggatagagg gtagttatcc acgtgaaacc gctaatgccc
       61 cgcaaagcct tgattcacgg ggctttccgg cccgctccaa aaactatcca cgtgaaatcg
      121 ctaatcaggg tacgtgaaat cgctaatcgg agtacgtgaa atcgctaata aggtcacgtg
      181 aaatcgctaa tcaaaaaggc acgtgagaac gctaatagcc ctttcagatc aacagcttgc
      241 aaacacccct cgctccggca agtagttaca gcaagtagta tgttcaatta gcttttcaat
      301 tatgaatata tatatcaatt attggtcgcc cttggcttgt ggacaatgcg ctacgcgcac
      361 cggctccgcc cgtggacaac cgcaagcggt tgcccaccgt cgagcgccag cgcctttgcc
      421 cacaacccgg cggccggccg caacagatcg ttttataaat tttttttttt gaaaaagaaa
      481 aagcccgaaa ggcggcaacc tctcgggctt ctggatttcc gatccccgga attagagatc
      541 ttggcaggat atattgtggt gtaacgttat cagcttgcat gccggtcgat ctagtaacat
      601 agatgacacc gcgcgcgata atttatccta gtttgcgcgc tatattttgt tttctatcgc
      661 gtattaaatg tataattgcg ggactctaat cataaaaacc catctcataa ataacgtcat
      721 gcattacatg ttaattatta catgcttaac gtaattcaac agaaattata tgataatcat
      781 cgcaagaccg gcaacaggat tcaatcttaa gaaactttat tgccaaatgt ttgaacgatc
      841 tgcttgactc taggggtcat cagatttcgg tgacgggcag gaccggacgg ggcggcaccg
      901 gcaggctgaa gtccagctgc cagaaaccca cgtcatgcca gttcccgtgc ttgaagccgg
      961 ccgcccgcag catgccgcgg ggggcatatc cgagcgcctc gtgcatgcgc acgctcgggt
     1021 cgttgggcag cccgatgaca gcgaccacgc tcttgaagcc ctgtgcctcc agggacttca
     1081 gcaggtgggt gtagagcgtg gagcccagtc ccgtccgctg gtggcggggg gagacgtaca
     1141 cggtggactc ggccgtccag tcgtaggcgt tgcgtgcctt ccagggaccc gcgtaggcga
     1201 tgccggcgac ctcgccgtcc acctcggcga cgagccaggg atagcgctcc cgcagacgga
     1261 cgaggtcgtc cgtccactcc tgcggttcct gcggctcggt acggaagttg accgtgcttg
     1321 tctcgatgta gtggttgacg atggtgcaga ccgccggcat gtccgcctcg gtggcacggc
     1381 ggatgtcggc cgggcgtcgt tctgggctca tggtagatcc cctcgatcga gttgagagtg
     1441 aatatgagac tctaattgga taccgagggg aatttatgga acgtcagtgg agcatttttg
     1501 acaagaaata tttgctagct gatagtgacc ttaggcgact tttgaacgcg caataatggt
     1561 ttctgacgta tgtgcttagc tcattaaact ccagaaaccc gcggctcagt ggctccttca
     1621 acgttgcggt tctgtcagtt ccaaacgtaa aacggcttgt cccgcgtcat cggcgggggt
     1681 cataacgtga ctcccttaat tctccgctca tgtatcgata acattaacgt ttacaatttc
     1741 gcgccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc
     1801 gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc
     1861 agggttttcc cagtcacgac gttgtaaaac gacggccagt gagcgcgcgt aatacgactc
     1921 actatagggc gaattgggta ccgggccccc cctcgaggtc gacggtatcg ataagcttga
     1981 tatcgaattc ctgcagccca tttaggtgac actatagaat atcactagta agctagcttg
     2041 catgccggtc ctgctgagcc tcgacatgtt gtcgcaaaat tcgccctgga cccgcccaac
     2101 gatttgtcgt cactgtcaag gtttgacctg cacttcattt ggggcccaca tacaccaaaa
     2161 aaatgctgca taattctcgg ggcagcaagt cggttacccg gccgccgtgc tggaccgggt
     2221 tgaatggtgc ccgtaacttt cggtagagcg gacggccaat actcaacttc aaggaatctc
     2281 acccatgcgc gccggcgggg aaccggagtt cccttcagtg agcgttatta gttcgccgct
     2341 cggtgtgtcg tagatactag cccctggggc acttttgaaa tttgaataag atttatgtaa
     2401 tcagtctttt aggtttgacc ggttctgccg ctttttttaa aattggattt gtaataataa
     2461 aacgcaattg tttgttattg tggcgctcta tcatagatgt cgctataaac ctattcagca
     2521 caatatattg ttttcatttt aatattgtac atataagtag tagggtacaa tcagtaaatt
     2581 gaacggagaa tattattcat aaaaatacga tagtaacggg tgatatattc attagaatga
     2641 accgaaaccg gcggtaagga tctgagctac acatgctcag gttttttaca acgtgcacaa
     2701 cagaattgaa agcaaatatc atgcgatcat aggcgtctcg catatctcat taaagcaggg
     2761 atcccgcatc agatctatca aacacataga tttacgaaga tctctaacat taaatcatca
     2821 gagagataag tttagggagc aagctctttc gcttacacag agaatcacag aggaaagaga
     2881 ctttaagaaa actacaactt ttttgaccac tgtgatttct ctctgcaagc gaaagtactt
     2941 aatcaccaac acagtagatt gatatatttg ggtttatttg tgagaagaag aattcactag
     3001 tggatcccgc actcgagtgt taatcagaaa aactcagatt aatctacaaa ttcgatcgca
     3061 caaactagaa actaacacca gatctagata gaaatcacaa atcgaagagt aattattcga
     3121 caaaactcaa attatttgaa caaatcggat gatatctatg aaaccctaat cgagaattaa
     3181 gatgatatct aacgatcaaa cccagaaaat cgtcttcgat ctaagattaa cagaatctaa
     3241 accaaagaac atatacgaaa ttgggatcga acgaaaacaa aatcgaagat tttgagagaa
     3301 taaggaacac agaaatttac cttgatcacg gtagagagaa ttgagagaaa gtttttaaga
     3361 ttttgagaaa ttgaaatctg aattgtgaag aagaagagct ctttgggtat tgttttatag
     3421 aagaagaaga agaaaagacg aggacgacta ggtcacgaga aagctaaggc ggtgaagcaa
     3481 tagctaataa taaaatgaca cgtgtattga gcgttgttta cacgcaaagt tgtttttggc
     3541 taattgcctt atttttaggt tgaggaaaag tatttgtgct ttgagttgat aaacacgact
     3601 cgtgtgtgcc ggctgcaacc actttgacgc cgtttattac tgactcgtcg gggggatcca
     3661 ttcggtcccc agattagcct tttcaatttc agaaagaatg ctaacccaca gatggttaga
     3721 gaggcttacg cagcaggtct catcaagacg atctacccga gcaataatct ccaggaaatc
     3781 aaataccttc ccaagaaggt taaagatgca gtcaaaagat tcaggactaa ctgcatcaag
     3841 aacacagaga aagatatatt tctcaagatc agaagtacta ttccagtatg gacgattcaa
     3901 ggcttgcttc acaaaccaag gcaagtaata gagattggag tctctaaaaa ggtagttccc
     3961 actgaatcaa aggccatgga gtcaaagatt caaatagagg acctaacaga actcgccgta
     4021 aagactggcg aacagttcat acagagtctc ttacgactca atgacaagaa gaaaatcttc
     4081 gtcaacatgg tggagcacga cacacttgtc tactccaaaa atatcaaaga tacagtctca
     4141 gaagaccaaa gggcaattga gacttttcaa caaagggtaa tatccggaaa cctcctcgga
     4201 ttccattgcc cagctatctg tcactttatt gtgaagatag tggaaaagga aggtggctcc
     4261 tacaaatgcc atcattgcga taaaggaaag gccatcgttg aagatgcctc tgccgacagt
     4321 ggtcccaaag atggaccccc acccacgagg agcatcgtgg aaaaagaaga cgttccaacc
     4381 acgtcttcaa agcaagtgga ttgatgtgat atctccactg acgtaaggga tgacgcacaa
     4441 tcccactatc cttcgcaaga cccttcctct atataaggaa gttcatttca tttggagaga
     4501 acacggggga cgagcttcta gaggatcaca agtttgtaca aaaaagctga acgagaaacg
     4561 taaaatgata taaatatcaa tatattaaat tagattttgc ataaaaaaca gactacataa
     4621 tactgtaaaa cacaacatat ccagtcatat tggcggcccc attaggcacc ccaggcttta
     4681 cactttatgc ttccggctcg tataatgtgt ggattttgag ttaggagccg tcgagatttt
     4741 caggagctaa ggaagctaaa atggagaaaa aaatcactgg atataccacc gttgatatat
     4801 cccaatggca tcgtaaagaa cattttgagg catttcagtc agttgctcaa tgtacctata
     4861 accagaccgt tcagctggat attacggcct ttttaaagac cgtaaagaaa aataagcaca
     4921 agttttatcc ggcctttatt cacattcttg cccgcctgat gaatgctcat ccggagttcc
     4981 gtatggcaat gaaagacggt gagctggtga tatgggatag tgttcaccct tgttacaccg
     5041 ttttccatga gcaaactgaa acgttttcat cgctctggag tgaataccac gacgatttcc
     5101 ggcagtttct acacatatat tcgcaagatg tggcgtgtta cggtgaaaac ctggcctatt
     5161 tccctaaagg gtttattgag aatatgtttt tcgtctcagc caatccctgg gtgagtttca
     5221 ccagttttga tttaaacgtg gccaatatgg acaacttctt cgcccccgtt ttcaccatgg
     5281 gcaaatatta tacgcaaggc gacaaggtgc tgatgccgct ggcgattcag gttcatcatg
     5341 ccgtttgtga tggcttccat gtcggcagaa tgcttaatga attacaacag tactgcgatg
     5401 agtggcaggg cggggcgtaa acgcgtggag ccggcttact aaaagccaga taacagtatg
     5461 cgtatttgcg cgctgatttt tgcggtataa gaatatatac tgatatgtat acccgaagta
     5521 tgtcaaaaag aggtatgcta tgaagcagcg tattacagtg acagttgaca gcgacagcta
     5581 tcagttgctc aaggcatata tgatgtcaat atctccggtc tggtaagcac aaccatgcag
     5641 aatgaagccc gtcgtctgcg tgccgaacgc tggaaagcgg aaaatcagga agggatggct
     5701 gaggtcgccc ggtttattga aatgaacggc tcttttgctg acgagaacag gggctggtga
     5761 aatgcagttt aaggtttaca cctataaaag agagagccgt tatcgtctgt ttgtggatgt
     5821 acagagtgat attattgaca cgcccggccg acggatggtg atccccctgg ccagtgcacg
     5881 tctgctgtca gataaagtct cccgtgaact ttacccggtg gtgcatatcg gggatgaaag
     5941 ctggcgcatg atgaccaccg atatggccag tgtgccggtc tccgttatcg gggaagaagt
     6001 ggctgatctc agccaccgcg aaaatgacat caaaaacgcc attaacctga tgttctgggg
     6061 aatataaatg tcaggctccc ttatacacag ccagtctgca cctcgaccat agtgactgga
     6121 tatgttgtgt tttacagtat tatgtagtct gttttttatg caaaatctaa tttaatatat
     6181 tgatatttat atcattttac gtttctcgtt cagctttctt gtacaaagtg gtgatcctag
     6241 ctttcgttcg tatcatcggt ttcgacaacg ttcgtcaagt tcaatgcatc agtttcattg
     6301 cgcacacacc agaatcctac tgagtttgag tattatggca ttgggaaaac tgtttttctt
     6361 gtaccatttg ttgtgcttgt aatttactgt gttttttatt cggttttcgc tatcgaactg
     6421 tgaaatggaa atggatggag aagagttaat gaatgatatg gtccttttgt tcattctcaa
     6481 attaatatta tttgtttttt ctcttatttg ttgtgtgttg aatttgaaat tataagagat
     6541 atgcaaacat tttgttttga gtaaaaatgt gtcaaatcgt ggcctctaat gaccgaagtt
     6601 aatatgagga gtaaaacact tgtagttgta ccattatgct tattcactag gcaacaaata
     6661 tattttcaga cctagaaaag ctgcaaatgt tactgaatac aagtatgtcc tcttgtgttt
     6721 tagacattta tgaactttcc tttatgtaat tttccagaat ccttgtcaga ttctaatcat
     6781 tgctttataa ttatagttat actcatggat ttgtagttga gtatgaaaat attttttaat
     6841 gcattttatg acttgccaat tgattgacaa catgcatcaa tcggagctcc agcttttgtt
     6901 ccctttagtg agggttaatt ccgagcttgg cgtaatcatg gtcatagctg tttcctgtgt
     6961 gaaattgtta tccgctcaca attccacaca acatacgagc cggaaghcat aaagtgtaaa
     7021 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
     7081 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
     7141 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     7201 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     7261 tcaggggata acgcaggaaa gaacatgaag gccttgacag gatatattgg cgggtaaact
     7321 aagtcgctgt atgtgtttgt ttgagatctc atgtgagcaa aaggccagca aaaggccagg
     7381 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     7441 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     7501 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     7561 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     7621 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     7681 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     7741 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     7801 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     7861 ggtatctgcg ctctgctgaa gccagttacc ttcggaagaa gagttggtag ctcttgatcc
     7921 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     7981 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     8041 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     8101 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatgt gtaacattgg
     8161 tctagtgatt atttgccgac taccttggtg atctcgcctt tcacgtagtg aacaaattct
     8221 tccaactgat ctgcgcgcga ggccaagcga tcttcttgtc caagataagc ctgcctagct
     8281 tcaagtatga cgggctgata ctgggccggc aggcgctcca ttgcccagtc ggcagcgaca
     8341 tccttcggcg cgattttgcc ggttactgcg ctgtaccaaa tgcgggacaa cgtaagcact
     8401 acatttcgct catcgccagc ccagtcgggc ggcgagttcc atagcgttaa ggtttcattt
     8461 agcgcctcaa atagatcctg ttcaggaacc ggatcaaaga gttcctccgc cgctggacct
     8521 accaaggcaa cgctatgttc tcttgctttt gtcagcaaga tagccagatc aatgtcgatc
     8581 gtggctggct cgaagatacc tgcaagaatg tcattgcgct gccattctcc aaattgcagt
     8641 tcgcgcttag ctggataacg ccacggaatg atgtcgtcgt gcacaacaat ggtgacttct
     8701 acagcgcgga gaatctcgct ctctccaggg gaagccgaag tttccaaaag gtcgttgatc
     8761 aaagctcgcc gcgttgtttc atcaagcctt acggtcaccg taaccagcaa atcaatatca
     8821 ctgtgtggct tcaggccgcc atccactgcg gagccgtaca aatgtacggc cagcaacgtc
     8881 ggttcgagat ggcgctcgat gacgccaact acctctgata gttgagtcga tacttcggcg
     8941 atcaccgctt ccctcataac accccttgta ttactgttta tgtaagcaga cagttttatt
     9001 gttcatgatg atatattttt atcttgtgca atgtaacatc agagattttg agacacaacg
     9061 tggctttgtt gaataaatcg aacttttgct gagttgaagg atcagatcac gcatcttccc
     9121 gacaacgcag accgttccgt ggcaaagcaa aagttcaaaa tcaccaactg gtccacctac
     9181 aacaaagctc tcatcaaccg tggctccctc actttctggc tggatgatgg ggcgattcag
     9241 gcgatcccca tccaacagcc cgccgtcgag cgggct
//
