LOCUS       sequence-430581-        1721 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mini-mutagenesis plasmid for lambda light chain YSD Fabs.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1721)
  AUTHORS   Petersen BM, Kirby MB, Chrispens KM, Irvin OM, Strawn IK, Haas CM, 
            Walker AM, Baumer ZT, Ulmer SA, Ayala E, Rhodes ER, Guthmiller JJ, 
            Steiner PJ, Whitehead TA
  TITLE     An integrated technology for quantitative wide mutational scanning 
            of human antibody Fab libraries.
  JOURNAL   Nat Commun. 2024 May 10;15(1):3974. doi: 10.1038/s41467-024-48072-z.
  PUBMED    38730230
REFERENCE   2  (bases 1 to 1721)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2024-05-10"; volume: "15(1):3974. doi"; pages: " 
            10.1038/s41467-024-48072-z"
COMMENT     Sequence Label: pMMP_lambda
FEATURES             Location/Qualifiers
     source          1..1721
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      137..725
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     626..645
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1019..1678)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1593..1612
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(join(1679..1721,1..60))
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
ORIGIN
        1 acgcccggta gtgatcttat ttcattatgg tgaaagttgg aacctcttac gtgccgatca
       61 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
      121 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
      181 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
      241 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
      301 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
      361 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
      421 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
      481 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
      541 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
      601 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
      661 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
      721 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
      781 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtgcggcc gccagggtag
      841 aagtcgctga tgaggcacac cagggtggcc ttgttggcct gcagctcctc ggaagacggt
      901 ggaaacaggg tcacagagac cagctacata taaggtctca caagggcccg caggtgttcc
      961 ccctggcccc cagcagcaag agcaccagcg gcggcggccg ccctcagcct taaaaaaatt
     1021 acgccccgcc ctgccactca tcgcagtact gttgtaattc attaagcatt ctgccgacat
     1081 ggaagccatc acaaacggca tgatgaacct gaatcgccag cggcatcagc accttgtcgc
     1141 cttgcgtata atatttgccc atggtgaaaa cgggggcgaa gaagttgtcc atattggcca
     1201 cgtttaaatc aaaactggtg aaactcaccc agggattggc tgagacgaaa aacatattct
     1261 caataaaccc tttagggaaa taggccaggt tttcaccgta acacgccaca tcttgcgaat
     1321 atatgtgtag aaactgccgg aaatcgtcgt ggtattcact ccagagcgat gaaaacgttt
     1381 cagtttgctc atggaaaacg gtgtaacaag ggtgaacact atcccatatc accagctcac
     1441 cgtctttcat tgccatacgg aattccggat gagcattcat caggcgggca agaatgtgaa
     1501 taaaggccgg ataaaacttg tgcttatttt tctttacggt ctttaaaaag gccgtaatat
     1561 ccagctgaac ggtctggtta taggtacatt gagcaactga ctgaaatgcc tcaaaatgtt
     1621 ctttacgatg ccattgggat atatcaacgg tggtatatcc agtgattttt ttctccattt
     1681 tagcttcctt agctcctgaa aatctcgata actcaaaaaa t
//
