LOCUS       sequence-430678-       21202 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression Td-CBEmax in rice cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 21202)
  AUTHORS   Fan T, Cheng Y, Wu Y, Liu S, Tang X, He Y, Liao S, Zheng X, Zhang T,
            Qi Y, Zhang Y
  TITLE     High performance TadA-8e derived cytosine and dual base editors with
            undetectable off-target effects in plants.
  JOURNAL   Nat Commun. 2024 Jun 14;15(1):5103. doi: 10.1038/s41467-024-49473-w.
  PUBMED    38877035
REFERENCE   2  (bases 1 to 21202)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2024-06-14"; volume: "15(1):5103. doi"; pages: " 
            10.1038/s41467-024-49473-w"
COMMENT     Sequence Label: pGEL855
FEATURES             Location/Qualifiers
     source          1..21202
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        178..523
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     466..486
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             596..1303
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     1009..1028
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             1325..2350
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     CDS             1325..2350
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     polyA_signal    2391..2565
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    2643..2667
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             3092..3886
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      5311..5899
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5800..5819
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6053..6070
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    6085..6225
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(6151..6170)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6311..6333
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      6569..6763
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(6829..7902)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(8331..8960)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    10261..10285
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     10488..10504
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    11080..11101
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11116..11121,11122..11139,11140..11146)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11154..11170
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11178..11194
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             11190..11597
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSAQLTLTKGNKSWRTSRGGPVPNSPYSESYYARSLAVVL
                     QRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWDAPCSGARLQGG
                     TRQCIARCMHCLHCGHAATFCNLHKLACVQL"
     primer_bind     11213..11233
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        11215..11233
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(11280..11299)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(11281..11299)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(11309..11325)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      complement(13247..13495)
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     CDS             complement(13511..13531)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(13574..13822)
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             complement(13853..14101)
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             complement(14102..14158)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(14171..14218)
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             complement(join(14219..18292,18293..18295,18296..18319))
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     RBS             14298..14306
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             complement(18344..18364)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(18965..18985)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     promoter        complement(19031..21028)
                     /label=Ubi promoter
                     /note="maize polyubiquitin gene promoter"
     primer_bind     complement(21075..21091)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    21099..21115
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(21123..21129,21130..21147,21148..21153))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    21168..21189
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
       61 gcgcggggag aggcggtttg cgtattggct agagcagctt gccaacatgg tggagcacga
      121 cactctcgtc tactccaaga atatcaaaga tacagtctca gaagaccaaa gggctattga
      181 gacttttcaa caaagggtaa tatcgggaaa cctcctcgga ttccattgcc cagctatctg
      241 tcacttcatc aaaaggacag tagaaaagga aggtggcacc tacaaatgcc atcattgcga
      301 taaaggaaag gctatcgttc aagatgcctc tgccgacagt ggtcccaaag atggaccccc
      361 acccacgagg agcatcgtgg aaaaagaaga cgttccaacc acgtcttcaa agcaagtgga
      421 ttgatgtgat atctccactg acgtaaggga tgacgcacaa tcccactatc cttcgcaaga
      481 cccttcctct atataaggaa gttcatttca tttggagagg acacgctgaa atcaccagtc
      541 tctctctaca aatctatctc tctcgagctt tcgcagatcc ggggggcaat gagatatggt
      601 gagcaagggc gaggaggata acatggccat catcaaggag ttcatgcgct tcaaggtgca
      661 catggagggc tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc
      721 ctacgagggc acccagaccg ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc
      781 ctgggacatc ctgtcccctc agttcatgta cggctccaag gcctacgtga agcaccccgc
      841 cgacatcccc gactacttga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat
      901 gaacttcgag gacggcggcg tggtgaccgt gacccaggac tcctccctgc aggacggcga
      961 gttcatctac aaggtgaagc tgcgcggcac caacttcccc tccgacggcc ccgtaatgca
     1021 gaagaagacc atgggctggg aggcctcctc cgagcggatg taccccgagg acggcgccct
     1081 gaagggcgag atcaagcaga ggctgaagct gaaggacggc ggccactacg acgctgaggt
     1141 caagaccacc tacaaggcca agaagcccgt gcagctgccc ggcgcctaca acgtcaacat
     1201 caagttggac atcacctccc acaacgagga ctacaccatc gtggaacagt acgaacgcgc
     1261 cgagggccgc cactccaccg gcggcatgga cgagctttac aagtcgggcg gaggtggctc
     1321 tgatatgaaa aagcctgaac tcaccgcgac gtctgtcgag aagtttctga tcgaaaagtt
     1381 cgacagcgtg tccgacctga tgcagctctc ggagggcgaa gaatctcgtg ctttcagctt
     1441 cgatgtagga gggcgtggat atgtcctgcg ggtaaatagc tgcgccgatg gtttctacaa
     1501 agatcgttat gtttatcggc actttgcatc ggccgcgctc ccgattccgg aagtgcttga
     1561 cattggggaa ttcagcgaga gcctgaccta ttgcatctcc cgccgtgcac agggtgtcac
     1621 gttgcaagac ctgcctgaaa ccgaactgcc cgctgttctg cagccggtcg cggaggccat
     1681 ggatgcgatc gctgcggccg atcttagcca gacgagcggg ttcggcccat tcggaccgca
     1741 aggaatcggt caatacacta catggcgtga tttcatatgc gcgattgctg atccccatgt
     1801 gtatcactgg caaactgtga tggacgacac cgtcagtgcg tccgtcgcgc aggctctcga
     1861 tgagctgatg ctttgggccg aggactgccc cgaagtccgg cacctcgtgc acgcggattt
     1921 cggctccaac aatgtcctga cggacaatgg ccgcataaca gcggtcattg actggagcga
     1981 ggcgatgttc ggggattccc aatacgaggt cgccaacatc ttcttctgga ggccgtggtt
     2041 ggcttgtatg gagcagcaga cgcgctactt cgagcggagg catccggagc ttgcaggatc
     2101 gccacgactc cgggcgtata tgctccgcat tggtcttgac caactctatc agagcttggt
     2161 tgacggcaat ttcgatgatg cagcttgggc gcagggtcga tgcgacgcaa tcgtccgatc
     2221 cggagccggg actgtcgggc gtacacaaat cgcccgcaga agcgcggccg tctggaccga
     2281 tggctgtgta gaagtactcg ccgatagtgg aaaccgacgc cccagcactc gtccgagggc
     2341 aaagaaatag agtagatgcc gaccgggatc tgtcgatcga caagctcgag tttctccata
     2401 ataatgtgtg agtagttccc agataaggga attagggttc ctatagggtt tcgctcatgt
     2461 gttgagcata taagaaaccc ttagtatgta tttgtatttg taaaatactt ctatcaataa
     2521 aatttctaat tcctaaaacc aaaatccagt actaaaatcc agatcccccg aattaattcg
     2581 gcgttaattc agtacattaa aaacgtccgc aatgtgttat taagttgtct aagcgtcaat
     2641 ttgtttacac cacaatatat cctgccacca gccagccaac agctccccga ccggcagctc
     2701 ggcacaaaat caccactcga tacaggcagc ccatcagtcc gggacggcgt cagcgggaga
     2761 gccgttgtaa ggcggcagac tttgctcatg ttaccgatgc tattcggaag aacggcaact
     2821 aagctgccgg gtttgaaaca cggatgatct cgcggagggt agcatgttga ttgtaacgat
     2881 gacagagcgt tgctgcctgt gatcaccgcg gtttcaaaat cggctccgtc gatactatgt
     2941 tatacgccaa ctttgaaaac aactttgaaa aagctgtttt ctggtattta aggttttaga
     3001 atgcaaggaa cagtgaattg gagttcgtct tgttataatt agcttcttgg ggtatcttta
     3061 aatactgtag aaaagaggaa ggaaataata aatggctaaa atgagaatat caccggaatt
     3121 gaaaaaactg atcgaaaaat accgctgcgt aaaagatacg gaaggaatgt ctcctgctaa
     3181 ggtatataag ctggtgggag aaaatgaaaa cctatattta aaaatgacgg acagccggta
     3241 taaagggacc acctatgatg tggaacggga aaaggacatg atgctatggc tggaaggaaa
     3301 gctgcctgtt ccaaaggtcc tgcactttga acggcatgat ggctggagca atctgctcat
     3361 gagtgaggcc gatggcgtcc tttgctcgga agagtatgaa gatgaacaaa gccctgaaaa
     3421 gattatcgag ctgtatgcgg agtgcatcag gctctttcac tccatcgaca tatcggattg
     3481 tccctatacg aatagcttag acagccgctt agccgaattg gattacttac tgaataacga
     3541 tctggccgat gtggattgcg aaaactggga agaagacact ccatttaaag atccgcgcga
     3601 gctgtatgat tttttaaaga cggaaaagcc cgaagaggaa cttgtctttt cccacggcga
     3661 cctgggagac agcaacatct ttgtgaaaga tggcaaagta agtggcttta ttgatcttgg
     3721 gagaagcggc agggcggaca agtggtatga cattgccttc tgcgtccggt cgatcaggga
     3781 ggatatcggg gaagaacagt atgtcgagct attttttgac ttactgggga tcaagcctga
     3841 ttgggagaaa ataaaatatt atattttact ggatgaattg ttttagtacc tagaatgcat
     3901 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg ctgatgaatc ccctaatgat
     3961 ttttatcaaa atcattaagt taaggtagat acacatcttg tcatatgatc aaatggtttc
     4021 gccaaaaatc aataatcaga caacaaaatg tgcgaactcg atattttaca cgactctctt
     4081 taccaattct gccccgaatt acacttaaaa cgactcaaca gcttaacgtt ggcttgccac
     4141 gccttacttg actgtaaaac tctcactctt accgaacttg gccgtaacct gccaaccaaa
     4201 gcgagaacaa aacataacat caaacgaatc gaccgattgt taggtaatcg tcacctccac
     4261 aaagagcgac tcgctgtata ccgttggcat gctagcttta tctgttcggg caatacgatg
     4321 cccattgtac ttgttgactg gtctgatatc cgtgagcaaa aacggcttat ggtattgcga
     4381 gcttcagtcg cactacacgg tcgttctgtt actctttatg agaaagcgtt cccgctttca
     4441 gagcaatgtt caaagaaagc tcatgaccaa tttctagccg accttgcgag cattctaccg
     4501 agtaacacca caccgctcat tgtcagtgat gctggcttta aagtgccatg gtataaatcc
     4561 gttgagaagc tgggttggta ctggttaagt cgagtaagag gaaaagtaca atatgcagac
     4621 ctaggagcgg aaaactggaa acctatcagc aacttacatg atatgtcatc tagtcactca
     4681 aagactttag gctataagag gctgactaaa agcaatccaa tctcatgcca aattctattg
     4741 tataaatctc gctctaaagg ccgaaaaaat cagcgctcga cacggactca ttgtcaccac
     4801 ccgtcaccta aaatctactc agcgtcggca aaggagccat ggattctagc aactaactta
     4861 cctgttgaaa ttcgaacacc caaacaactt gttaatatct attcgaagcg aatgcagatt
     4921 gaagaaacct tccgagactt gaaaagtcct gcctacggac taggcctacg ccatagccga
     4981 acgagcagct cagagcgttt tgatatcatg ctgctaatcg ccctgatgct tcaactaaca
     5041 tgttggcttg cgggcgttca tgctcagaaa caaggttggg acaagcactt ccaggctaac
     5101 acagtcagaa atcgaaacgt actctcaaca gttcgcttag gcatggaagt tttgcggcat
     5161 tctggctaca caataacaag ggaagactta ctcgtggctg caaccctact agctcaaaat
     5221 ttattcacac atggttacgc tttggggaaa ttatgagggg atctctcagc actgagcgtc
     5281 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     5341 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     5401 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct
     5461 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     5521 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     5581 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     5641 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     5701 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     5761 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     5821 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     5881 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     5941 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     6001 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
     6061 agtgagcgag gaagcggaag agggcctgat gcggtatttt ctccttacgc atctgtgcgg
     6121 tatttcacac cgcatatggt gcactctcag tacaatctgc tctgatgccg catagttaag
     6181 ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca
     6241 acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct
     6301 gtgaccgttt ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg
     6361 aggcagggtg ccttgatgtg ggcgccggcg gtcgagtggc gacggcgcgg cttgtccgcg
     6421 ccctggtaga ttgcctggcc gtaggccagc catttttgag cggccagcgg ccgcgatagg
     6481 ccgacgcgaa gcggcggggc gtagggagcg cagcgaccga agggtaggcg ctttttgcag
     6541 ctcttgggct gtgcgctggc cagacagtta tgcacaggcc aggcgggttt taagagtttt
     6601 aataagtttt aaagagtttt aggcggaaaa atcgcctttt ttctctttta tatcagtcac
     6661 ttacatgtgt gaccggttcc caatgtacgg ctttgggttc ccaatgtacg ggttccggtt
     6721 cccaatgtac ggctttgggt tcccaatgta cgtgctatcc acaggaaaga ggccttttcg
     6781 acctttttcc cctgctaggg caatttgccc tagcatctgc tccgtacatt aggaaccggc
     6841 ggatgcttcg ccctcgatca ggttgcggta gcgcatgact aggatcgggc cagcctgccc
     6901 cgcctcctcc ttcaaatcgt actccggcag gtcatttgac ccgatcagct tgcgcacggt
     6961 gaaacagaac ttcttgaact ctccggcgct gccactgcgt tcgtagatcg tcttgaacaa
     7021 ccatctggct tctgccttgc ctgcggcgcg gcgtgccagg cggtagagaa aacggccgat
     7081 gccgggatcg atcaaaaagt aatcggggtg aaccgtcagc acgtccgggt tcttgccttc
     7141 tgtgatctcg cggtacatcc aatcagctag ctcgatctcg atgtactccg gccgcccggt
     7201 ttcgctcttt acgatcttgt agcggctaat caaggcttca ccctcggata ccgtcaccag
     7261 gcggccgttc ttggccttct tcgtacgctg catggcaacg tgcgtggtgt ttaaccgaat
     7321 gcaggtttct accaggtcgt ctttctgctt tccgccatcg gctcgccggc agaacttgag
     7381 tacgtccgca acgtgtggac ggaacacgcg gccgggcttg tctcccttcc cttcccggta
     7441 tcggttcatg gattcggtta gatgggaaac cgccatcagt accaggtcgt aatcccacac
     7501 actggccatg ccggccggcc ctgcggaaac ctctacgtgc ccgtctggaa gctcgtagcg
     7561 gatcacctcg ccagctcgtc ggtcacgctt cgacagacgg aaaacggcca cgtccatgat
     7621 gctgcgacta tcgcgggtgc ccacgtcata gagcatcgga acgaaaaaat ctggttgctc
     7681 gtcgcccttg ggcggcttcc taatcgacgg cgcaccggct gccggcggtt gccgggattc
     7741 tttgcggatt cgatcagcgg ccgcttgcca cgattcaccg gggcgtgctt ctgcctcgat
     7801 gcgttgccgc tgggcggcct gcgcggcctt caacttctcc accaggtcat cacccagcgc
     7861 cgcgccgatt tgtaccgggc cggatggttt gcgaccgctc acgccgattc ctcgggcttg
     7921 ggggttccag tgccattgca gggccggcag gcaacccagc cgcttacgcc tggccaaccg
     7981 cccgttcctc cacacatggg gcattccacg gcgtcggtgc ctggttgttc ttgattttcc
     8041 atgccgcctc ctttagccgc taaaattcat ctactcattt attcatttgc tcatttactc
     8101 tggtagctgc gcgatgtatt cagatagcag ctcggtaatg gtcttgcctt ggcgtaccgc
     8161 gtacatcttc agcttggtgt gatcctccgc cggcaactga aagttgaccc gcttcatggc
     8221 tggcgtgtct gccaggctgg ccaacgttgc agccttgctg ctgcgtgcgc tcggacggcc
     8281 ggcacttagc gtgtttgtgc ttttgctcat tttctcttta cctcattaac tcaaatgagt
     8341 tttgatttaa tttcagcggc cagcgcctgg acctcgcggg cagcgtcgcc ctcgggttct
     8401 gattcaagaa cggttgtgcc ggcggcggca gtgcctgggt agctcacgcg ctgcgtgata
     8461 cgggactcaa gaatgggcag ctcgtacccg gccagcgcct cggcaacctc accgccgatg
     8521 cgcgtgcctt tgatcgcccg cgacacgaca aaggccgctt gtagccttcc atccgtgacc
     8581 tcaatgcgct gcttaaccag ctccaccagg tcggcggtgg cccatatgtc gtaagggctt
     8641 ggctgcaccg gaatcagcac gaagtcggct gccttgatcg cggacacagc caagtccgcc
     8701 gcctggggcg ctccgtcgat cactacgaag tcgcgccggc cgatggcctt cacgtcgcgg
     8761 tcaatcgtcg ggcggtcgat gccgacaacg gttagcggtt gatcttcccg cacggccgcc
     8821 caatcgcggg cactgccctg gggatcggaa tcgactaaca gaacatcggc cccggcgagt
     8881 tgcagggcgc gggctagatg ggttgcgatg gtcgtcttgc ctgacccgcc tttctggtta
     8941 agtacagcga taaccttcat gcgttcccct tgcgtatttg tttatttact catcgcatca
     9001 tatacgcagc gaccgcatga cgcaagctgt tttactcaaa tacacatcac ctttttagac
     9061 ggcggcgctc ggtttcttca gcggccaagc tggccggcca ggccgccagc ttggcatcag
     9121 acaaaccggc caggatttca tgcagccgca cggttgagcc gtgcgcgggc ggctcgaaca
     9181 cgtacccggc cgcgatcatc tccgcctcga tctcttcggt aatgaaaaac ggttcgtcct
     9241 ggccgtcctg gtgcggtttc atgcttgttc ctcttggcgt tcattctcgg cggccgccag
     9301 ggcgtcggcc tcggtcaatg cgtcctcacg gaaggcaccg cgccgcctgg cctcggtggg
     9361 cgtcacttcc tcgctgcgct caagtgcgcg gtacagggtc gagcgatgca cgccaagcag
     9421 tgcagccgcc tctttcacgg tgcggccttc ctggtcgatc agctcgcggg cgtgcgcgat
     9481 ctgtgccggg gtgagggtag ggcgggggcc aaacttcacg cctcgggcct tggcggcctc
     9541 gcgcccgctc cgggtgcggt cgatgattag ggaacgctcg aactcggcaa tgccggcgaa
     9601 cacggtcaac accatgcggc cggccggcgt ggtggtgtcg gcccacggct ctgccaggct
     9661 acgcaggccc gcgccggcct cctggatgcg ctcggcaatg tccagtaggt cgcgggtgct
     9721 gcgggccagg cggtctagcc tggtcactgt cacaacgtcg ccagggcgta ggtggtcaag
     9781 catcctggcc agctccgggc ggtcgcgcct ggtgccggtg atcttctcgg aaaacagctt
     9841 ggtgcagccg gccgcgtgca gttcggcccg ttggttggtc aagtcctggt cgtcggtgct
     9901 gacgcgggca tagcccagca ggccagcggc ggcgctcttg ttcatggcgt aatgtctccg
     9961 gttctagtcg caagtattct actttatgcg actaaaacac gcgacaagaa aacgccagga
    10021 aaagggcagg gcggcagcct gtcgcgtaac ttaggacttg tgcgacatgt cgttttcaga
    10081 agacggctgc actgaacgtc agaagccgac tgcactatag cagcggaggg gttggatcaa
    10141 agtactttga tcccgagggg aaccctgtgg ttggcatgca catacaaatg gacgaacgga
    10201 taaacctttt cacgcccttt taaatatccg attattctaa taaacgctct tttctcttag
    10261 gtttacccgc caatatatcc tgtcaaacac tgatagttta aactgaaggc gggaaacgac
    10321 aatctgatcc aagctcaagc tgctctagca ttcgccattc aggctgcgca actgttggga
    10381 agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc
    10441 aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc
    10501 cagtgccaca ctgagctcga tatcggcgcg ccccgggagc tcagcggcaa aacacaccta
    10561 gactagattt gttttgctaa cccaattgat attaattata tatgattaat atttatatgt
    10621 atatggaatt ggttaataaa atgcatctgg ttcatcaaag aattataaag acacgtgaca
    10681 ttcatttagg ataagaaata tggatgatct ctttctctta ttcagataat tagtaattac
    10741 acataacaca caactttgat gcccacatta tagtgattag catgtcacta tgtgtgcatc
    10801 cttttatttc atacattaat taacttggcc aatccagaag atggacaagt ctaggctcga
    10861 gtacctgcac cagccgggaa tcgaacccgg gtctgtaccg tggcagggta ctattctacc
    10921 actagaccac tggtgctttg ttgcaccgac tcggtgccac tttgctgttt ccagcaaagt
    10981 tgataacgga ctagccttat ttaaacttgc tatgctgttt ccagcatagc tcttaaactg
    11041 agaccaccgg tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca
    11101 ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag
    11161 cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc gcgcaattaa
    11221 ccctcactaa agggaacaaa agctggagga cctcgagggg gggcccggta cccaattcgc
    11281 cctatagtga gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg
    11341 aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc
    11401 gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg
    11461 aatgggacgc gccctgtagc ggcgcccgtc tccagggggg cacccgtcag tgcattgcgc
    11521 gatgcatgca ctgcctccat tgcggtcacg ccgccacgtt ttgcaatctt cacaagttgg
    11581 cctgtgtcca actgtaaggt ctcctgcacc agccgggaat cgaacccggg tctgtaccgt
    11641 ggcagggtac tattctacca ctagaccact ggtgctttgt tcctgcaggc tgcacacaca
    11701 attaccgaag catggtcagc aactatgaac atgaaattat aacaatacca agcaagtaca
    11761 gcagaaatca gtcaatcaaa ttattccaat atgttagata cacgatctaa accacaataa
    11821 catcagtgta cctatcaaca ccaacagtga cctaacaaaa tagagagtcg caccgtgctc
    11881 gagttcacaa aaataatctg caggtctatg aactactaga attccatcgg caaactgatt
    11941 caataactac tagcactatc ccgagagcac ataaggcagg ttaattaaca taggctagaa
    12001 tttacaggtg caacagttga taagataggc ataagcaagt aaaatatcct gaaagctatg
    12061 ctatgtaatc actaatcaac aagacgcagc ccttagatta aacatgaaca gagtagaatc
    12121 tatgcaacta gagggactga atcaaaaaca acctaagcaa aatatacctg aagctgctac
    12181 ttggatcaac gagaaaaaca gagactaaat cctactgatt gaacagatcg gaacaaatca
    12241 caaccctagc acagttctac ttctagatcc gaaccaataa atacttggac cacgaacagc
    12301 atcgctcata atcacagatc cagcatacca cgcgaacccc atctaaccct gttcaaatct
    12361 accacagatc gcaaaacgga gactaacaac aacatctcac aatcacgaac aggccgggaa
    12421 atcaaatcaa acgcgaggaa cggagaggat cagaaaatcg cataccttga gaaggcgatt
    12481 cgacgcttcg cttcgagggg agattttttt ttctctgcga ttttggggat tcgattgatg
    12541 gaggaagagg cgaggagggg gtggggtatt tataggtggg gaggggggga ctcgtgagtg
    12601 ggcccagggg tgcggtgggt ggggcccagt gagaatatag acaaataaag agagggagag
    12661 gcggtttggg gagtccgtcc ccaccacgca aacgcgagaa ggaaagaaag gaacggaaag
    12721 ggggcgggtc cagacggcgc cgcggagata tcgaggaggt ggaaagctga tgccgtttgc
    12781 tcacggcggg gttggtggcc gttaacacgc agcgaagcgg cgttactgcc gtgtacgttt
    12841 tgtgaccttt cgaaagagag ccccagccca agaaaggctg gccaggtttt tttgtactgt
    12901 gtacgattac gacggaagaa aacatactcg ttgttttgta ttggtagata ctgggccgta
    12961 gtaaaattag aatcagttgt taattggtta ttatatatac catcttaagc taccaaaatt
    13021 aataaagttg tgatggctat ggtatatttt ggtagtgaat tatattgatt tggtatatat
    13081 tagaatcaat ccaaccacaa cacaaccaaa aatacctgaa gctcaatatt gatggtgctt
    13141 ttcgactatc tagcaaatgt ggtgaatggg gctttataat tgaagcagcc caggatgcac
    13201 tttgtgcaga gactgatgtg ctccggcgcg ccgtccgagc tcagcgctta tctttaatca
    13261 tattccatag tccataccat agcacataca gtagttatat gctgcagaag agatccaaca
    13321 aaacattcac aatggattat agaaacattt gtttattcat tataatgtga tcttacattc
    13381 atttaatatt agaaaaagcc acaaattcat aacacaacaa gccaagaaaa aaacacaaac
    13441 ttaagcacac aagcttttta tttgacacac caaatatttc atcttcatct tcatatactc
    13501 gagaagctca cacttttctt ttctttttgg gctcaaactc tgaaccatca gcagtacgct
    13561 ttgaacctcc cgacagcatc ttgattttat tctcgccgtt tgaatcttgt attactaacg
    13621 cccatggctt gtattctgga gcatcggagg tcaataacat cacattctcg tcggtagact
    13681 cgtcataagc agtatgcact agaatgtccg attcgggctt gttgccgatc acctcctcca
    13741 cttcttcagg gagcatcagg atgctctctt ggatgaccaa ctgcttccca gtctctttct
    13801 caataatatc actcaggttc gtcgaaccgc ctgacccccc cgacccgccg gatagcattt
    13861 tgattttgtt ctcaccgtta gagtcctgga tgaccaacgc ccacggcttg tactcaggtg
    13921 catcagaagt caaaagcatg acattctcat ccgtgctttc gtcgtacgcg gtatgaacaa
    13981 gaatgtcaga ttcaggcttg ttgccgatga cctcctccac ctcctccgga agcatgagaa
    14041 tgctctcttg tataaccagc tgtttcccag tttccttctc gattatatct gagagattag
    14101 taggcccagg attttcttcc acgtcaccgg cctgcttaag tagcgagaaa ttggtggcgc
    14161 ctgatcctgc cttcttcttc ttcgcctgcc ccgccttctt cgtcgccgct ggccgcttgt
    14221 cgcccccgag ctgagacagg tcgatgcgcg tctcgtagag gccggtaatc gactggtgga
    14281 tgagggtcgc gtccaggacc tccttagtgc ttgtgtacct cttgcgatcg atagttgtgt
    14341 cgaagtactt gaaagcagca ggggcgccga ggttcgtcag ggtgaagaga tgaatgatat
    14401 tctcagcctg ctccctgatt ggcttgtcgc ggtgcttgtt gtacgcggag aggaccttat
    14461 ccagattcgc gtcggccagg atcacgcgct tggagaactc ggaaatctgc tcaatgatct
    14521 cgtcgaggta atgcttgtgc tgctcgacga acagctgctt ctgctcgttg tcctcggggc
    14581 tgcccttgag cttctcgtag tgggaggcca ggtagaggaa gttcacatac ttggacggca
    14641 gagccagctc gttccccttc tgcagctcgc cagcggaagc cagcatccgc ttcctgccgt
    14701 tctccagctc gaagagtgag tacttgggga gcttaatgat caggtccttc ttcacctcct
    14761 tgtagccctt cgcctccagg aaatcgatcg ggttcttctc gaagctggag cgctccataa
    14821 tcgtgatccc cagcagctcc ttcacgctct tgagcttctt ggacttgccc ttctcaacct
    14881 tcgccacaac caggaccgag taggccacag tggggctgtc gaacccgccg tacttcttcg
    14941 gatcccagtc cttcttgcgg gcgatgagct tgtcgctgtt ccgcttaggc agaattgact
    15001 ccttagagaa cccgccagtc tggacctctg tcttcttgac gatattcact tgtggcatgg
    15061 agagaacctt cctgacggtc gcgaaatccc tgcccttgtc ccacacgatc tcccccgtct
    15121 cgccgttcgt ctcgatgagg gggcgcttcc ggatctcgcc attggccaga gtgatctctg
    15181 tcttgaagaa attcataatg ttagagtaga agaagtactt ggcggtagcc ttgccaatct
    15241 cctgctccga cttggcgatc atcttcctca catcgtaaac cttgtagtcc ccgtacacga
    15301 actcgctctc gagctttggg tacttcttga tcagagctgt gccgaccacc gcgttcaggt
    15361 acgcgtcatg ggcatggtgg taattgttga tctcccgaac cttgtagaac tggaaatcct
    15421 tcctgaagtc ggagacgagc tttgacttca gggtgatgac cttcacctcg cggatcagct
    15481 tgtcattctc atcgtactta gtgttcatcc gtgagtcgag aatctgcgca acgtgcttag
    15541 tgatctgccg tgtctcgacc agctgcctct tgatgaagcc cgccttgtcc agctcagaga
    15601 gcccgcccct ctcagccttt gtgaggttat cgaacttccg ctgcgtgatc agcttggcat
    15661 tcaggagctg gcgccagtag ttcttcatct tcttaacgac ctcctctgaa ggaacattat
    15721 cagacttgcc ccggttcttg tccgacctgg tgaggacctt gttgtcaatg gagtcatcct
    15781 tcaggaatga ctgtggaacg atatgatcga cgtcgtaatc gctgagcctg ttaatatcca
    15841 gctcctggtc cacatacata tcgcggccat tctggaggta gtacaggtag agcttctcat
    15901 tctgcagctg cgtgttctcc accgggtgct ccttgaggat ctgggacccc agctccttaa
    15961 tgccctcctc gatcctcttc atcctctcgc gtgagttctt ctggcccttc tgcgtggtct
    16021 gattctcccg ggccatctca atgacgatgt tctcaggctt gtgcctgccc atgaccttca
    16081 ccagctcgtc cacaaccttc acggtctgca gaatcccctt cttgatagct ggcgagccag
    16141 cgaggttcgc gatatgctcg tgcagcgagt ccccctggcc gctcacctga gccttctgga
    16201 tatcctcctt gaatgtgagg ctgtcatcgt gaatcagctg catgaaattg cggttcgcga
    16261 agccatcgct cttcaggaag tcgaggatcg tcttcccgga ctgcttgtcc cgaatgccgt
    16321 tgatgagctt cctgctcagc ctcccccagc cggtgtacct cctcctcttg agctgcttca
    16381 tgaccttgtc atcgaagaga tgggcgtaag tcttcaggcg ctcctcgatc atctcccggt
    16441 cctcgaacag agtgagtgtc agcacaatgt cctcgaggat atcctcattc tcctcgttgt
    16501 ccaggaagtc cttatcctta atgatcttca ggagatcgtg gtaggtcccc agggaggcgt
    16561 tgaagcggtc ctcaacgcca gagatctcga ccgaatcgaa gcactcaatc ttcttgaagt
    16621 agtcctcctt gagctgctta accgtgacct tccggttggt cttgaacagg aggtccacga
    16681 tggccttctt ctgctcccca gacaggaaag ccggcttcct catgccctcg gtcacatact
    16741 tcaccttagt cagctcgttg tagactgtga agtactcgta caggagcgag tgcttaggga
    16801 gcaccttctc atttggcagg ttcttgtcga aattcgtcat cctctcgatg aacgactgag
    16861 cgctagcgcc cttgtcgacc acctcctcga agttccacgg cgtgatcgtc tcctctgact
    16921 tgcgggtcat ccaagcgaag cgggagttgc ccctagcgag tgggccgacg tagtacggga
    16981 tcctgaaagt cagaatcttc tcgatcttct cgcggttatc cttgaggaaa gggtagaagt
    17041 cctcctgcct cctcaggata gcgtgcagct ccccgagatg aatctggtgt gggatgctgc
    17101 cgttatcgaa tgtccgctgc ttcctcagga ggtcctcgcg attgagcttc accagcagct
    17161 cctccgtgcc gtccatcttc tccagaatcg gcttgatgaa cttgtagaac tcctcctgag
    17221 aggccccgcc gtcaatgtac ccagcgtagc cgttcttcga ctgatcgaag aagatctcct
    17281 tgtacttctc ggggagctgc tgcctgacca gcgccttcag gagggtcaga tcctgatggt
    17341 gctcgtcgta gcgcttgatc atggaggctg agagcggagc cttcgtaatc tcggtgttca
    17401 ccctgagaat atcagacagg aggatggcgt ccgacagatt cttggcagcg aggaacaggt
    17461 ccgcgtactg atcgccgatc tgggccagga ggttatccag gtcatcgtcg tatgtgtcct
    17521 tggagagctg cagcttggcg tcctcagcga gatcgaaatt cgacttgaag ttgggcgtga
    17581 gccccaggct gagcgcaatg agattcccga acaggccgtt cttcttctcg cccggcagct
    17641 gggcgatcag gttctcgagg cgccgagact tcgagagcct agcggacagg atagccttcg
    17701 cgtcgacgcc tgacgcatta atggggttct cctcgaagag ctggttgtac gtctgcacga
    17761 gctggatgaa cagcttgtca acatcgctat tgtccgggtt gagatccccc tcgatcagga
    17821 aatggcccct gaacttaatc atgtgggcca gagcgaggta gatcaggcgg aggtccgcct
    17881 tatctgtgga gtccacgagc ttcttccgca gatggtagat cgtagggtac ttctcgtggt
    17941 aggcaacctc gtcgacaatg ttgccgaaga ttggatgccg ctcgtgcttc ttatcctcct
    18001 ccacgaggaa tgactcctcc agcctgtgga agaaagaatc gtcaaccttc gccatctcgt
    18061 tggagaaaat ctcctgcagg tagcagatgc gattcttcct gcgcgtgtac cgcctgcggg
    18121 cggtgcgctt gagccgcgta gcctcagccg tctcgccgct gtcgaacagg agagcgccaa
    18181 tgagattctt cttgatggaa tgccgatcgg tgttgcccag gaccttgaac ttctttgagg
    18241 gcaccttgta ctcgtcggtg atcacggccc agccaacaga gttagtccca atagcgaggc
    18301 cgatcgagta cttcttgtca gcagctggca ccccgtggat gccaaccttc ctcttcttct
    18361 tcggagccat cgaacccccg ctgctgcccc cgctgctttc aggtgttgcg ctctcgcttg
    18421 tgccaggtgt ctcagagcca gaggagcctc cgctagatcc tccggagttg atggagctct
    18481 gggccttctt ctgagcattg aacacctgtc taggcatccg atagaaatcg cacagcaggg
    18541 cggcacattc atctgccagg attccctcgg taatttcgac gcggtgattc atgccggggt
    18601 agttcagcac gttcatcagg gagcctgcgg cgcctctttt tgagttcctc acgccaaaca
    18661 ccacgcggcc gatcctagag tggatcatgg cgccggcgca catcacgcaa ggctcgaatg
    18721 tcacgtacag ggtggcgtca atcagtctgt agttctgcat gaccaggccg ccctgtctca
    18781 gggccataat ttcggcatgg gctgttgggt cgtgcaggcc gatggctctg agccagccct
    18841 cgccgatcac tctattgttc agcaccagca cggctcccac aggcacgcgc ctctcatccc
    18901 gtgccctctt ggccagggtc agggcatgtc tcatccagta ctcgtgggaa aactccacct
    18961 cagagacttt ccgcttcttc tttggtgact cgaactcgct tccgtcggct gtccgtttca
    19021 ttcctgcagg ctgcagaagt aacaccaaac aacagggtga gcatcgacaa aagaaacagt
    19081 accaagcaaa taaatagcgt atgaaggcag ggctaaaaaa atccacatat agctgctgca
    19141 tatgccatca tccaagtata tcaagatcaa aataattata aaacatactt gtttattata
    19201 atagataggt actcaaggtt agagcatatg aatagatgct gcatatgcca tcatgtatat
    19261 gcatcagtaa aacccacatc aacatgtata cctatcctag atcgatattt ccatccatct
    19321 taaactcgta actatgaaga tgtatgacac acacatacag ttccaaaatt aataaataca
    19381 ccaggtagtt tgaaacagta ttctactccg atcttgaacg aatgaacgac cgcccaacca
    19441 caccacatca tcacaaccaa gcgaacaaaa agcatctctg tatatgcatc agtaaaaccc
    19501 gcatcaacat gtatacctat cctagatcga tatttccatc catcatcttc aattcgtaac
    19561 tatgaatatg tatggcacac acatacagat ccaaaattaa taaatccacc aggtagtttg
    19621 aaacagaatt aattctactc cgatcttgaa cgaccgccca accagaccac atcatcacaa
    19681 ccaagacaaa aaaaagcatg aaaagatgac ccgacaaaca agtgcacggc atatattgaa
    19741 ataaaggaaa agggcaaacc aaaccctatg caacgaaaca aaaaaaatca tgaaatcgat
    19801 cccgtctgcg gaacggctag agccatccca ggattcccca aagagaaaca ctggcaagtt
    19861 agcaatcaga acgtgtctga cgtacaggtc gcatccgtgt acgaacgcta gtagcacgga
    19921 tctaacacaa acacggatct aacacaaaca tgaacagaag tagaactacc gggccctaac
    19981 catggaccgg aacgccgatc ttgagaaggt agagaggggg gggggggagg acgagcggcg
    20041 taccttgaag cggaggtgcc gacgggtgga tttgggggag ttctggttgt gtgtgtgtgc
    20101 gctccgaaca acacgaggtt ggggaaagag ggtgtggagg gggtgtctat ttattacggc
    20161 gggcgaggaa gggaaagcga aggagcggtg ggaaaggaat cccccgtagc tgccggtgcc
    20221 gtgagaggag gaggaggccg cctgccgtgc cggctcacgt ctgccgctcc gccacgcaat
    20281 ttctggatgc cgacagcgga gcaagtccaa cggtggagcg gaactctcga gaggggtcca
    20341 gaggcagcga cagagatgcc gtgccgtctg cttcgcttgg cccgacgcga cgctgctggt
    20401 tcgctggttg gtgtccgtta gactcgtcga cggcgtttaa caggctggca ttatctactc
    20461 gaaacaagaa aaatgtttcc ttagtttttt taatttctta aagggtattt gtttaatttt
    20521 tagtcacttt attttattct attttatatc taaattatta aataaaaaaa ctaaaataga
    20581 gttttagttt tcttaattta gaggctaaaa tagaataaaa tagatgtact aaaaaaatta
    20641 gtctataaaa accattaacc ctaaacccta aatggatgta ctaataaaat ggatgaagta
    20701 ttatataggt gaagctattt gcaaaaaaaa aggagaacac atgcacacta aaaagataaa
    20761 actgtagagt cctgttgtca aaatactcaa ttgtccttta gaccatgtct aactgttcat
    20821 ttatatgatt ctctaaaaca ctgatattat tgtagtacta tagattatat tattcgtaga
    20881 gtaaagttta aatatatgta taaagataga taaactgcac ttcaaacaag tgtgacaaaa
    20941 aaaatatgtg gtaatttttt ataacttaga catgcaatgc tcaccttatc tcaagagagg
    21001 ggcacgaccg ggtcacgctg cactgcagct ccggcgcgcc gatccccggg taccgagctc
    21061 gaattcgtaa tcatgtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
    21121 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
    21181 ctcacattaa ttgcgttgcg ct
//
