LOCUS       sequence-43174-e         841 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd gen lentiviral vector expressing a fusion protein of Lamp1, 
            mRFP, and a FLAG tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 841)
  AUTHORS   Zoncu R, Bar-Peled L, Efeyan A, Wang S, Sancak Y, Sabatini DM
  TITLE     mTORC1 senses lysosomal amino acids through an inside-out mechanism 
            that requires the vacuolar H-ATPase.
  JOURNAL   Science. 2011 Nov 4;334(6056):678-83.
  PUBMED    22053050
REFERENCE   2  (bases 1 to 841)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2011-11-4"; volume: "334(6056)"; pages: "678-83"
COMMENT     Sequence Label: LAMP1-mRFP-FLAG
FEATURES             Location/Qualifiers
     source          1..841
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      34..43
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      38..47
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gagctggttt agtgaaccgt cagatccgct agcgccacca tggcggcccc gggcgcccgg
       61 cggccgctgc tcctgttgct gctggcaggc cttgcacaca gcgccccagc actgttcgag
      121 gtgaaagaca acaacggcac agcgtgtata atggccagct tctctgcctc ctttctgacc
      181 acctatgagg ctggacatgt ttctaaggtc tcgaatatga ccctgccagc ctctgcagaa
      241 gtcctgaaga atagcagctc ttgtggtgaa aagaatgctt ctgagcccac cctcgcaatc
      301 acctttggag aaggatattt actgaaactc accttcacaa aaaacacaac acgttacagt
      361 gtccagcaca tgtatttcac atataacctg tcagacacac aattctttcc caatgccagc
      421 tccaaagggc ccgacactgt ggattccaca actgacatca aggcagacat caacaaaaca
      481 taccgatgtg tcagcgacat cagggtctac atgaagaatg tgaccattgt gctctgggac
      541 gctactatcc aggcctacct gccgagtagc aacttcagca aggaagagac acgctgccca
      601 caggatcaac cttccccaac tactgggcca cccagcccct caccaccact tgtgcccaca
      661 aaccccagtg tgtccaagta caatgtgact ggtgacaatg gaacctgcct gctggcctct
      721 atggcactgc aactcaacat cacctacatg aagaaggaca acacgactgt gaccagagca
      781 ttcaacatca acccaagtga caaatatagt gggacttgcg gtgcccagtt ggtgaccctg
      841 a
//
