LOCUS       sequence-43185-e         732 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 732)
  AUTHORS   Park HS, Hohn MJ, Umehara T, Guo LT, Osborne EM, Benner J, Noren CJ,
            Rinehart J, Soll D
  TITLE     Expanding the genetic code of Escherichia coli with phosphoserine.
  JOURNAL   Science. 2011 Aug 26;333(6046):1151-4.
  PUBMED    21868676
REFERENCE   2  (bases 1 to 732)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2011-08-26"; volume: "333(6046)"; pages: "1151-4"
COMMENT     Sequence Label: pMYO
FEATURES             Location/Qualifiers
     source          1..732
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(376..403)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(496..582)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(613..630)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 aaaatcctta gctttcgcta ggatctgcag gtcgactcta gaggatcctg gagccggggg
       61 tgggatttga acccacgtaa ggcggatcta gagtccgctg cctaacccct agactacccc
      121 ggcgaattca gcgttacaag tattacacaa agttttttat gttgagaata tttttttgat
      181 ggggcgccac ttatttttga tcgttcgctc aaagaagcgg cgccagggtg gtttttcttt
      241 tcaccagtga gacgggcaac agaacgccat gagcggcctc atttcttatt ctgagttaca
      301 acagtccgca ccgctgccgg tagctccttc cggtgggcgc ggggcataga tctcaaaaga
      361 gtttgtagaa acgcaaaaag gccatccgtc aggatggcct tctgcttaat ttgatgcctg
      421 gcagttttat ggcgggcgtc ctgcccgcca ccctccgggc cgttgcttcg caacgttcaa
      481 atccgctccc ggcggatttg tcctactcag gagagcgttc accgacaaac aacagataaa
      541 acgaaaggcc cagtctttcg actgagcctt tcgttttatt tgatgcctgg cagttcccta
      601 cggcgcgcct tagtgatggt gatggtgatg accagaacca ccctggtaac ccagttcttt
      661 gtatttagca gcgatgtctt tacggaacag ttccagagct ttgttcatag caccctgagc
      721 gtccgcaccg aa
//
