LOCUS       sequence-431854-       11532 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of SARS-CoV-2 Spike_Lambda in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11532)
  AUTHORS   Broderick K, Moutaoufik MT, Saccon T, Malty R, Amin S, Phanse S, 
            Joseph TP, Zilocchi M, Hosseinnia A, Istace Z, Hajikarimlou M, Abrar
            S, Fisher J, Brassard R, Perera R, Kumar A, Aoki H, Rahmatbakhsh M, 
            Jessulat M, Kobasa D, Dehne F, Prasad B, Gagarinova A, Joanne 
            Lemieux M, Cochrane A, Houry WA, Aly KA, Golshani A, Babu M
  TITLE     Human protein interaction networks of ancestral and variant 
            SARS-CoV-2 in organ-specific cells and bodily fluids.
  JOURNAL   Nat Commun. 2025 Jul 1;16(1):5784. doi: 10.1038/s41467-025-60949-1.
  PUBMED    40593736
REFERENCE   2  (bases 1 to 11532)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2025-07-1"; volume: "16(1):5784. doi"; pages: " 
            10.1038/s41467-025-60949-1"
COMMENT     Sequence Label: pLD-puro-Cc-SARS-CoV-2_Spike_Lambda-VA
FEATURES             Location/Qualifiers
     source          1..11532
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(2..26)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             106..129
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             166..189
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             202..219
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             229..249
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             262..282
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             295..360
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    408..525
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        574..1084
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     1003..1021
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             1106..1705
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(1106..1125)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     1602..1622
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             1833..2066
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    2138..2259
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2175..2194)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2229..2248
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      2299..2434
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(2454..2473)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2455..2473)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2483..2500)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2483..2499)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2492..2514)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      2641..3096
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2728..2747)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2938..2959
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3122..3226
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3227..3295,3296..4087)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3445..3464)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4258..4846
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4747..4766
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5000..5017
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5134..5155
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5170..5175,5176..5193,5194..5200)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5208..5224
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5213..5235
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5232..5248
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5232..5248
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     5267..5287
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        5269..5287
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        5315..5541
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             5542..5722
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    5769..5894
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6387..6620
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             6805..6849
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     primer_bind     7147..7167
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     promoter        7456..7659
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     7610..7630
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     7656..7680
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    7711..7735
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tacccaactt tcttgtacaa agtggttgat tctagagcga tcgccagaga gctgttcaac
       61 ctgctgggcg agaaccagcc ccccgtggtg atcaaggcga tcgcctggag ccaccctcag
      121 ttcgagaagg gaggaggaag cggcggaggc agcggaggag gaagctggag ccacccgcag
      181 ttcgagaaag gcggaagcgg ccatcaccat caccatcacc ctgcaggaga aaatctgtac
      241 ttccagggga gaggaggcag cgagaacctg tactttcagg ggcctgcagg aagagactac
      301 aaagaccatg acggtgatta taaagatcat gacatcgatt acaaggatga cgatgacaag
      361 tagttcgaat tctcgacctc gagacaaatg gcagtattca tccacaattt taaaagaaaa
      421 ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata
      481 caaactaaag aattacaaaa acaaattaca aaaattcaaa attttcgggt ttattacagg
      541 gacagcagag atccactttg gccgcggctc gagggggttg gggttgcgcc ttttccaagg
      601 cagccctggg tttgcgcagg gacgcggctg ctctgggcgt ggttccggga aacgcagcgg
      661 cgccgaccct gggtctcgca cattcttcac gtccgttcgc agcgtcaccc ggatcttcgc
      721 cgctaccctt gtgggccccc cggcgacgct tcctgctccg cccctaagtc gggaaggttc
      781 cttgcggttc gcggcgtgcc ggacgtgaca aacggaagcc gcacgtctca ctagtaccct
      841 cgcagacgga cagcgccagg gagcaatggc agcgcgccga ccgcgatggg ctgtggccaa
      901 tagcggctgc tcagcagggc gcgccgagag cagcggccgg gaaggggcgg tgcgggaggc
      961 ggggtgtggg gcggtagtgt gggccctgtt cctgcccgcg cggtgttccg cattctgcaa
     1021 gcctccggag cgcacgtcgg cagtcggctc cctcgttgac cgaatcaccg acctctctcc
     1081 ccagggggat ccaccggagc ttaccatgac cgagtacaag cccacggtgc gcctcgccac
     1141 ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc
     1201 cacgcgccac accgtcgatc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact
     1261 cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc
     1321 ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg
     1381 cccgcgcatg gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct
     1441 cctggcgccg caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc
     1501 cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga
     1561 gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga
     1621 gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg
     1681 catgacccgc aagcccggtg cctgacgccc gccccacgac ccgcagcgcc cgaccgaaag
     1741 gagcgcacga ccccatgcat cggtaccttt aagaccaatg acttacaagg cagctgtaga
     1801 tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag
     1861 acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga
     1921 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
     1981 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
     2041 ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt
     2101 ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgtttattg cagcttataa
     2161 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     2221 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc tctagctatc
     2281 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     2341 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta
     2401 ttccagaagt agtgaggagg cttttttgga ggcctaggga cgtacccaat tcgccctata
     2461 gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac tgggaaaacc
     2521 ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata
     2581 gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggg
     2641 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
     2701 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
     2761 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
     2821 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
     2881 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
     2941 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
     3001 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
     3061 acgcgaattt taacaaaata ttaacgctta caatttaggt ggcacttttc ggggaaatgt
     3121 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     3181 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     3241 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     3301 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     3361 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     3421 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
     3481 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     3541 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     3601 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     3661 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     3721 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     3781 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     3841 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     3901 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     3961 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     4021 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     4081 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     4141 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     4201 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     4261 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     4321 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     4381 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     4441 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     4501 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     4561 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     4621 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     4681 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     4741 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     4801 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     4861 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     4921 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     4981 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg
     5041 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc
     5101 cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc
     5161 accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata
     5221 acaatttcac acaggaaaca gctatgacca tgattacgcc aagcgcgcaa ttaaccctca
     5281 ctaaagggaa caaaagctgg agctgcaagc ttaatgtagt cttatgcaat actcttgtag
     5341 tcttgcaaca tggtaacgat gagttagcaa catgccttac aaggagagaa aaagcaccgt
     5401 gcatgccgat tggtggaagt aaggtggtac gatcgtgcct tattaggaag gcaacagacg
     5461 ggtctgacat ggattggacg aaccactgaa ttgccgcatt gcagagatat tgtatttaag
     5521 tgcctagctc gatacataaa cgggtctctc tggttagacc agatctgagc ctgggagctc
     5581 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
     5641 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
     5701 tcagtgtgga aaatctctag cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac
     5761 cagaggagct ctctcgacgc aggactcggc ttgctgaagc gcgcacggca agaggcgagg
     5821 ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg aggctagaag gagagagatg
     5881 ggtgcgagag cgtcagtatt aagcggggga gaattagatc gcgatgggaa aaaattcggt
     5941 taaggccagg gggaaagaaa aaatataaat taaaacatat agtatgggca agcagggagc
     6001 tagaacgatt cgcagttaat cctggcctgt tagaaacatc agaaggctgt agacaaatac
     6061 tgggacagct acaaccatcc cttcagacag gatcagaaga acttagatca ttatataata
     6121 cagtagcaac cctctattgt gtgcatcaaa ggatagagat aaaagacacc aaggaagctt
     6181 tagacaagat agaggaagag caaaacaaaa gtaagaccac cgcacagcaa gcggccgctg
     6241 atcttcagac ctggaggagg agatatgagg gacaattgga gaagtgaatt atataaatat
     6301 aaagtagtaa aaattgaacc attaggagta gcacccacca aggcaaagag aagagtggtg
     6361 cagagagaaa aaagagcagt gggaatagga gctttgttcc ttgggttctt gggagcagca
     6421 ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac aggccagaca attattgtct
     6481 ggtatagtgc agcagcagaa caatttgctg agggctattg aggcgcaaca gcatctgttg
     6541 caactcacag tctggggcat caagcagctc caggcaagaa tcctggctgt ggaaagatac
     6601 ctaaaggatc aacagctcct ggggatttgg ggttgctctg gaaaactcat ttgcaccact
     6661 gctgtgcctt ggaatgctag ttggagtaat aaatctctgg aacagatttg gaatcacacg
     6721 acctggatgg agtgggacag agaaattaac aattacacaa gcttaataca ctccttaatt
     6781 gaagaatcgc aaaaccagca agaaaagaat gaacaagaat tattggaatt agataaatgg
     6841 gcaagtttgt ggaattggtt taacataaca aattggctgt ggtatataaa attattcata
     6901 atgatagtag gaggcttggt aggtttaaga atagtttttg ctgtactttc tatagtgaat
     6961 agagttaggc agggatattc accattatcg tttcagaccc acctcccaac cccgagggga
     7021 cccgacaggc ccgaaggaat agaagaagaa ggtggagaga gagacagaga cagatccatt
     7081 cgattagtga acggatctcg acggtatcga tcacgagact agcctcgagc ggccgccccc
     7141 ttcaccgagg gcctatttcc catgattcct tcatatttgc atatacgata caaggctgtt
     7201 agagagataa ttggaattaa tttgactgta aacacaaaga tattagtaca aaatacgtga
     7261 cgtagaaagt aataatttct tgggtagttt gcagttttaa aattatgttt taaaatggac
     7321 tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat
     7381 tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc
     7441 atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt
     7501 gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac
     7561 caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc
     7621 ggtaggcgtg tacggtggga ggtctatata agcagagctc gtttagtgaa ccgtcagatc
     7681 gcctggagac gccatccacg ctgtacgcgt acaagtttgt acaaaaaagt tggcatgttc
     7741 gtgttcctgg tgctgctgcc actggtgagc tcccagtgcg tgaacctgac caccagaacc
     7801 cagctgccac ctgcctacac caactccttc accaggggcg tgtactaccc agacaaggtg
     7861 ttcagatcca gcgtgctgca ctctacccag gacctgttcc tgccattctt ctctaacgtg
     7921 acctggttcc acgccatcca cgtgtctgga accaacgtga tcaagagatt cgacaaccca
     7981 gtgctgccat tcaacgacgg cgtgtacttc gcttctaccg agaagtctaa catcatcagg
     8041 ggttggatct tcggaaccac cctggactct aagacccagt ctctgctgat cgtgaacaac
     8101 gctaccaacg tggtgatcaa ggtgtgcgag ttccagttct gcaacgaccc attcctggga
     8161 gtgtactacc acaagaacaa caagtcttgg atggaaagcg agttcagggt gtacagctct
     8221 gctaacaact gcaccttcga gtacgtgtct cagcctttcc tgatggacct ggaaggaaag
     8281 cagggaaact tcaagaacct gagagagttc gtgttcaaga acatcgacgg ttacttcaag
     8341 atatacagca agcacacccc aatcaacctg gtgagggacc tgccacaggg tttctctgct
     8401 ctggagcccc tggtggacct gccaatcggc atcaacatca ccaggttcca gaccctgctg
     8461 gctctgcaca acagcagctc tggatggacc gccggcgccg ctgcttacta cgtgggatac
     8521 ctgcagccaa ggaccttcct gctgaagtac aacgaaaacg gcaccatcac cgacgccgtg
     8581 gactgcgctc tggacccact gtctgagacc aagtgcaccc tgaagtcttt caccgtggaa
     8641 aagggaatct accagaccag caacttccgc gtgcagccca ccgagtctat cgtgagattc
     8701 ccaaacatca ccaacctgtg cccattcgga gaagtgttca acgctaccag gttcgccagc
     8761 gtgtacgctt ggaacaggaa gagaatcagc aactgcgtgg ccgactactc tgtgctgtac
     8821 aactctgcct ccttctccac cttcaagtgc tacggcgtgt ccccaaccaa gctgaacgac
     8881 ctgtgcttca ccaacgtgta cgctgactcc ttcgtgatca gaggagacga agtgagacag
     8941 atcgctcccg gtcagaccgg aaagatcgcc gactacaact acaagctgcc agacgacttc
     9001 accggttgcg tgatcgcttg gaactctaac aacctggact ctaaggtggg aggcaactac
     9061 aactaccagt acaggctgtt ccgcaagagc aacctgaagc ctttcgaaag ggacatcagc
     9121 accgaaatct accaggctgg atctacccca tgcaacggag tggaaggatt caactgctac
     9181 agccctctgc agtcttacgg attccagcca accaacggag tgggatacca gccatacaga
     9241 gtggtggtgc tgtctttcga gctgctgcac gctcctgcta ccgtgtgcgg accaaagaag
     9301 tctaccaacc tggtgaagaa caagtgcgtg aacttcaact tcaacggact gaccggaacc
     9361 ggagtgctga ccgaatccaa caagaagttc ctgcctttcc agcagttcgg tagagacatc
     9421 gccgacacca ccgacgctgt gagagaccca cagaccctgg aaatcctgga catcaccccc
     9481 tgctctttcg gaggagtgag cgtgatcacc ccaggaacca acacctccaa ccaggtggcc
     9541 gtgctgtacc agggcgtgaa ctgcaccgaa gtgccagtgg ccatccacgc tgaccagctg
     9601 accccaacct ggagagtgta cagcaccgga tctaacgtgt tccagacccg tgccggctgc
     9661 ctgatcggag ccgagcacgt gaacaactcc tacgaatgcg acatcccaat cggagctggc
     9721 atctgcgctt cttaccagac ccagaccaac agcccaagaa gggccagatc cgtggcctct
     9781 cagagcatca tcgcctacac catgagcctg ggcgctgaaa acagcgtggc ttacagcaac
     9841 aactctatcg ccatcccaac caacttcacc atctctgtga ccaccgaaat cctgccagtg
     9901 agcatgacca agaccagcgt ggactgcacc atgtacatct gcggagactc taccgaatgc
     9961 tctaacctgc tgctgcagta cggcagcttc tgcacccagc tgaaccgcgc tctgaccgga
    10021 atcgctgtgg agcaggacaa gaacacccag gaagtgttcg ctcaggtgaa gcagatatac
    10081 aagaccccac ctatcaagga cttcggagga ttcaacttct ctcagatcct gcccgaccca
    10141 tctaagccct ctaagaggtc tttcatcgaa gacctgctgt tcaacaaggt gaccctggcc
    10201 gacgccggtt tcatcaagca gtacggtgac tgcctgggag acatcgccgc tagagacctg
    10261 atctgcgccc agaagttcaa cggtctgaac gtgctgccac cactgctgac cgacgagatg
    10321 atcgcccagt acacctctgc cctgctggct ggaaccatca cctctggttg gaccttcgga
    10381 gctggcgccg ccctgcagat cccattcgct atgcagatgg cctaccgttt caacggtatc
    10441 ggagtgaccc agaacgtgct gtacgaaaac cagaagctga tcgctaacca gttcaactct
    10501 gctatcggaa agatccagga ctccctgtcc tctaccgctt ctgccctggg aaagctgcag
    10561 gacgtggtga accagaacgc ccaggccctg aacaccctgg tgaagcagct gtcttctaac
    10621 ttcggagcca tctcctctgt gctgaacgac atcctgtcta ggctggacaa ggtggaagcc
    10681 gaggtgcaga tcgacagact gatcaccgga agactgcagt ctctgcagac ctacgtgacc
    10741 cagcagctga tcagggccgc tgaaatcaga gcttctgcca acctggctgc caccaagatg
    10801 tccgaatgcg tgctgggcca gtctaagagg gtggacttct gcggaaaggg ataccacctg
    10861 atgtccttcc cacagtctgc tccacacgga gtggtgttcc tgcacgtgac ctacgtgccc
    10921 gcccaggaaa agaacttcac caccgcccca gctatctgcc acgacggaaa ggctcacttc
    10981 cccagggagg gagtgttcgt gtctaacgga acccactggt tcgtgaccca gaggaacttc
    11041 tacgagccac agatcatcac caccgacaac accttcgtgt ctggaaactg cgacgtggtg
    11101 atcggaatcg tgaacaacac cgtgtacgac ccactgcagc cagaactgga ctctttcaag
    11161 gaggaactgg acaagtactt caagaaccac acctctccag acgtggacct gggagacatc
    11221 tccggaatca acgcctctgt ggtgaacatc cagaaggaaa tcgacagact gaacgaggtg
    11281 gccaagaacc tgaacgaatc tctgatcgac ctgcaggaac tgggaaagta cgagcagtac
    11341 atcaagtggc cctggtacat ctggctgggt ttcatcgctg gtctgatcgc tatcgtgatg
    11401 gtgaccatca tgctgtgctg catgacctct tgctgctctt gcctgaaggg atgctgctct
    11461 tgcggatctt gctgcaagtt cgacgaggac gactccgagc ctgtgctgaa gggtgtgaag
    11521 ctgcactaca cc
//
