LOCUS       sequence-431927-       16376 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses FnCpf1 and RecAb recombination system for genome editing 
            in Acinetobacter baumannii.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16376)
  AUTHORS   Wang S, Ding Y, Rong H, Wang Y
  TITLE     The Development of a CRISPR-FnCpf1 System for Large-Fragment 
            Deletion and Multiplex Gene Editing in Acinetobacter baumannii.
  JOURNAL   Curr Issues Mol Biol. 2024 Jan 5;46(1):570-584. doi: 
            10.3390/cimb46010037.
  PUBMED    38248339
REFERENCE   2  (bases 1 to 16376)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Curr Issues Mol Biol"; date: "2024-01-5- 5"; volume: "46"; issue: 
            "1"; pages: "570-584"
COMMENT     Sequence Label: pFnCpfAb-apr
FEATURES             Location/Qualifiers
     source          1..16376
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(38..55)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      208..294
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      386..413
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        join(678..683,684..699,700..706)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    714..730
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             join(5047..5133,5134..6468)
                     /codon_start=1
                     /gene="Bacillus subtilis sacB"
                     /product="secreted levansucrase that renders bacterial 
                     growth sensitive to sucrose"
                     /label=SacB
                     /note="negative selection marker"
                     /translation="MNIKKFAKQATVLTFTTALLAGGATQAFAKETNQKPYKETYGISH
                     ITRHDMLQIPEQQKNEKYQVPEFDSSTIKNISSAKGLDVWDSWPLQNADGTVANYHGYH
                     IVFALAGDPKNADDTSIYMFYQKVGETSIDSWKNAGRVFKDSDKFDANDSILKDQTQEW
                     SGSATFTSDGKIRLFYTDFSGKHYGKQTLTTAQVNVSASDSSLNINGVEDYKSIFDGDG
                     KTYQNVQQFIDEGNYSSGDNHTLRDPHYVEDKGHKYLVFEANTGTEDGYQGEESLFNKA
                     YYGKSTSFFRQESQKLLQSDKKRTAELANGALGMIELNDDYTLKKVMKPLIASNTVTDE
                     IERANVFKMNGKWYLFTDSRGSKMTIDGITSNDIYMLGYVSNSLTGPYKPLNKTGLVLK
                     MDLDPNDVTFTYSHFAVPQAKGNNVVITSYMTNRGFYADKQSTFAPSFLLNIKGKKTSV
                     VKDSILEQGQLTVNK"
     primer_bind     complement(6507..6526)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(6530..6546)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6531..6547)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     CDS             6685..7485
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRAALGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPHSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      7925..8319
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
     oriT            8661..8748
                     /label=RSF1010 oriT
                     /note="origin of transfer of the broad-host-range plasmid 
                     RSF1010 (Scholz et al., 1989)"
     CDS             9987..10958
                     /codon_start=1
                     /gene="repB"
                     /product="replication protein B of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepB
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRRTALD
                     EYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
     CDS             11469..12308
                     /codon_start=1
                     /gene="repA"
                     /product="replication protein A of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepA
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
     CDS             12295..13146
                     /codon_start=1
                     /gene="repC"
                     /product="replication protein C of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepC
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
     CDS             complement(13423..14505)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     14467..14486
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(14506..14583)
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     promoter        join(14813..14818,14819..14834,14835..14841)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    14849..14865
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 agcttggctg ttttggcgga tgagagaaga ttttcagcct gatacagatt aaatcagaac
       61 gcagaagcgg tctgataaaa cagaatttgc ctggcggcag tagcgcggtg gtcccacctg
      121 accccatgcc gaactcagaa gtgaaacgcc gtagcgccga tggtagtgtg gggtctcccc
      181 atgcgagagt agggaactgc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg
      241 gcctttcgtt ttatctgttg tttgtcggtg aacgctctcc tgagtaggac aaatccgccg
      301 ggagcggatt tgaacgttgc gaagcaacgg cccggagggt ggcgggcagg acgcccgcca
      361 taaactgcca ggcatcaaat taagcagaag gccatcctga cggatggcct ttttgcgttt
      421 ctacaaactc ttttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
      481 ataaccctgg atccgggctt atcgactgca cggtgcacca atgcttctgg cgtcaggcag
      541 ccatcggaag ctgtggtatg gctgtgcagg tcgtaaatca ctgcataatt cgtgtcgctc
      601 aaggcgcact cccgttctgg ataatgtttt ttgcgccgac atcataacgg ttctggcaaa
      661 tattctgaaa tgagctgttg acaattaatc atcggctcgt ataatgtgtg gaattgtgag
      721 cggataacaa tttcacacag gaaacagaat tcatgtccat ctaccaagag tttgtgaata
      781 aatactccct gtccaagacc ctccgttttg agctgatccc ccaaggcaag accctcgaaa
      841 acatcaaggc acgcggcctc atcctggatg acgaaaagcg cgctaaggat tacaagaagg
      901 caaagcagat catcgacaag taccaccagt tcttcatcga agagatcctg tcctccgtgt
      961 gcatctccga ggacctgctc cagaactact ccgatgtcta cttcaagctc aagaagtccg
     1021 atgacgataa cctgcagaag gacttcaagt ccgctaagga taccatcaag aagcagatct
     1081 ccgaatacat caaggattcc gagaagttca agaacctctt caaccagaac ctgatcgacg
     1141 caaagaaggg ccaggaatcc gatctcatcc tgtggctcaa gcagtccaag gataacggca
     1201 tcgagctctt caaggccaac tccgacatca ccgacatcga tgaagctctg gagatcatca
     1261 agtccttcaa gggctggacc acctacttca agggcttcca cgaaaaccgc aagaacgtgt
     1321 actcctccaa cgatatccca acctctatca tctaccgcat cgtcgacgat aacctgccaa
     1381 agttcctcga aaacaaggca aagtacgagt ccctgaagga taaggcccca gaagctatca
     1441 actacgagca gatcaagaag gacctggccg aagagctcac cttcgacatc gattacaaga
     1501 cctctgaagt gaaccagcgc gtcttctccc tcgatgaagt gttcgagatc gccaacttca
     1561 acaactacct gaaccagtcc ggcatcacca agttcaacac catcatcggc ggcaagttcg
     1621 tcaacggcga aaacaccaag cgcaagggca tcaacgagta catcaacctc tactcccagc
     1681 agatcaacga taagaccctg aagaagtaca agatgtccgt gctcttcaag cagatcctgt
     1741 ccgacaccga atccaagtcc ttcgtcatcg acaagctgga ggacgattcc gatgtggtca
     1801 ccaccatgca gtccttctac gaacagatcg cagccttcaa gaccgtggaa gagaagtcca
     1861 tcaaggagac cctctccctg ctcttcgacg atctgaaggc tcagaagctg gatctctcca
     1921 agatctactt caagaacgac aagtccctga ccgatctctc ccagcaggtc ttcgacgatt
     1981 actccgtgat cggcaccgca gtcctggaat acatcaccca gcagatcgcc ccaaagaacc
     2041 tcgataaccc atccaagaag gaacaggagc tgatcgccaa gaagaccgaa aaggctaagt
     2101 acctgtccct cgagaccatc aagctggctc tcgaagagtt caacaagcac cgcgacatcg
     2161 ataagcagtg ccgcttcgaa gagatcctcg caaacttcgc tgcaatccca atgatcttcg
     2221 acgaaatcgc acagaacaag gataacctgg cccagatctc catcaagtac cagaaccagg
     2281 gcaagaagga tctgctccag gcctccgctg aggacgatgt gaaggcaatc aaggacctgc
     2341 tcgatcagac caacaacctg ctccacaagc tgaagatctt ccacatctcc cagtccgaag
     2401 acaaggccaa catcctcgac aaggatgagc acttctacct ggtgttcgaa gagtgctact
     2461 tcgaactcgc taacatcgtc ccactgtaca acaagatccg caactacatc acccagaagc
     2521 catactccga tgaaaagttc aagctcaact tcgagaactc caccctggca aacggctggg
     2581 acaagaacaa ggaaccagat aacaccgcca tcctcttcat caaggacgat aagtactacc
     2641 tgggcgtgat gaacaagaag aacaacaaga tcttcgacga taaggccatc aaggaaaaca
     2701 agggcgaggg ctacaagaag atcgtgtaca agctgctccc aggcgctaac aagatgctcc
     2761 caaaggtctt cttctccgca aagtccatca agttctacaa cccatccgaa gatatcctgc
     2821 gcatccgcaa ccactccacc cacaccaaga acggctcccc acagaagggc tacgaaaagt
     2881 tcgagttcaa catcgaagac tgccgcaagt tcatcgattt ctacaagcag tccatctcca
     2941 agcacccaga gtggaaggac ttcggcttcc gcttctccga tacccagcgc tacaactcca
     3001 tcgatgaatt ctaccgcgaa gtggagaacc agggctacaa gctgaccttc gaaaacatct
     3061 ccgagtccta catcgattcc gtggtcaacc agggcaagct gtacctcttc cagatctaca
     3121 acaaggactt ctccgcttac tccaagggcc gcccaaacct gcacaccctc tactggaagg
     3181 cactcttcga cgaacgcaac ctgcaggatg tggtctacaa gctcaacggc gaagcagagc
     3241 tgttctaccg caagcagtcc atcccaaaga agatcaccca cccagccaag gaagcaatcg
     3301 ccaacaagaa caaggataac ccaaagaagg aatccgtgtt cgagtacgac ctgatcaagg
     3361 ataagcgctt caccgaggac aagttcttct tccactgccc aatcaccatc aacttcaagt
     3421 cctccggcgc caacaagttc aacgatgaaa tcaacctgct cctgaaggag aaggctaacg
     3481 acgtgcacat cctgtccatc gatcgcggcg aacgccacct cgcctactac accctggtcg
     3541 acggcaaggg caacatcatc aagcaggaca ccttcaacat catcggcaac gatcgcatga
     3601 agaccaacta ccacgacaag ctggccgcta tcgagaagga ccgcgattcc gctcgcaagg
     3661 attggaagaa gatcaacaac atcaaggaaa tgaaggaagg ctacctctcc caggtggtcc
     3721 acgaaatcgc taagctggtg atcgagtaca acgcaatcgt ggtcttcgaa gacctgaact
     3781 tcggcttcaa gcgcggccgc ttcaaggtgg agaagcaggt ctaccagaag ctggaaaaga
     3841 tgctcatcga gaagctgaac tacctcgtgt tcaaggacaa cgaattcgat aagaccggcg
     3901 gcgtcctccg tgcataccag ctgaccgccc cattcgagac cttcaagaag atgggcaagc
     3961 agaccggcat catctactac gtgccagctg gcttcacctc taagatctgc ccagtgaccg
     4021 gcttcgtcaa ccagctctac ccaaagtacg aatccgtctc caagtcccag gagttcttct
     4081 ccaagttcga caagatctgc tacaacctgg ataagggcta cttcgaattc tccttcgact
     4141 acaagaactt cggcgataag gcagccaagg gcaagtggac catcgcatcc ttcggctccc
     4201 gcctcatcaa cttccgcaac tccgacaaga accacaactg ggatacccgc gaagtgtacc
     4261 caaccaagga actggagaag ctcctgaagg attactccat cgaatacggc cacggcgagt
     4321 gcatcaaggc tgcaatctgc ggcgaatccg acaagaagtt cttcgcaaag ctgacctctg
     4381 tgctcaacac catcctgcag atgcgcaact ccaagaccgg caccgagctg gattacctca
     4441 tctccccagt ggccgacgtc aacggcaact tcttcgattc ccgccaggct ccaaagaaca
     4501 tgccacagga cgctgatgca aacggcgcct accacatcgg tctgaagggt ctcatgctcc
     4561 tgggtcgcat caagaacaac caggaaggca agaagctgaa tctcgtcatt aagaacgaag
     4621 aatactttga atttgtccag aaccgcaata actaagtcga cctgcaggca tgcgcaactt
     4681 tatgcccatg caacagaaac tataaaaaat acagagaatg aaaagaaaca gatagatttt
     4741 ttagttcttt aggcccgtag tctgcaaatc cttttatgat tttctatcaa acaaaagagg
     4801 aaaatagacc agttgcaatc caaacgagag tctaatagaa tgaggtcgaa aagtaaatcg
     4861 cgcgggtttg ttactgataa agcaggcaag acctaaaatg tgtaaagggc aaagtgtata
     4921 ctttggcgtc accccttaca tattttaggt ctttttttat tgtgcgtaac taacttgcca
     4981 tcttcaaaca ggagggctgg aagaagcaga ccgctaacac agtacataaa aaaggagaca
     5041 tgaacgatga acatcaaaaa gtttgcaaaa caagcaacag tattaacctt tactaccgca
     5101 ctgctggcag gaggcgcaac tcaagcgttt gcgaaagaaa cgaaccaaaa gccatataag
     5161 gaaacatacg gcatttccca tattacacgc catgatatgc tgcaaatccc tgaacagcaa
     5221 aaaaatgaaa aatatcaagt tcctgagttc gattcgtcca caattaaaaa tatctcttct
     5281 gcaaaaggcc tggacgtttg ggacagctgg ccattacaaa acgctgacgg cactgtcgca
     5341 aactatcacg gctaccacat cgtctttgca ttagccggag atcctaaaaa tgcggatgac
     5401 acatcgattt acatgttcta tcaaaaagtc ggcgaaactt ctattgacag ctggaaaaac
     5461 gctggccgcg tctttaaaga cagcgacaaa ttcgatgcaa atgattctat cctaaaagac
     5521 caaacacaag aatggtcagg ttcagccaca tttacatctg acggaaaaat ccgtttattc
     5581 tacactgatt tctccggtaa acattacggc aaacaaacac tgacaactgc acaagttaac
     5641 gtatcagcat cagacagctc tttgaacatc aacggtgtag aggattataa atcaatcttt
     5701 gacggtgacg gaaaaacgta tcaaaatgta cagcagttca tcgatgaagg caactacagc
     5761 tcaggcgaca accatacgct gagagatcct cactacgtag aagataaagg ccacaaatac
     5821 ttagtatttg aagcaaacac tggaactgaa gatggctacc aaggcgaaga atctttattt
     5881 aacaaagcat actatggcaa aagcacatca ttcttccgtc aagaaagtca aaaacttctg
     5941 caaagcgata aaaaacgcac ggctgagtta gcaaacggcg ctctcggtat gattgagcta
     6001 aacgatgatt acacactgaa aaaagtgatg aaaccgctga ttgcatctaa cacagtaaca
     6061 gatgaaattg aacgcgcgaa cgtctttaaa atgaacggca aatggtatct gttcactgac
     6121 tcccgcggat caaaaatgac gattgacggc attacgtcta acgatattta catgcttggt
     6181 tatgtttcta attctttaac tggcccatac aagccgctga acaaaactgg ccttgtgtta
     6241 aaaatggatc ttgatcctaa cgatgtaacc tttacttact cacacttcgc tgtacctcaa
     6301 gcgaaaggaa acaatgtcgt gattacaagc tatatgacaa acagaggatt ctacgcagac
     6361 aaacaatcaa cgtttgcgcc tagcttcctg ctgaacatca aaggcaagaa aacatctgtt
     6421 gtcaaagaca gcatccttga acaaggacaa ttaacagtta acaaataaaa acgcaaaaga
     6481 aaatgccgat tatggtgcac tctcagtaca atctgctctg atgccgtatc gataccgtcg
     6541 acctcgaggg ggggcccggt accatcccaa tacgcgtcaa ttcacggatc cggttcatgt
     6601 gcagctccat cagcaaaagg ggatgataag tttatcacca ccgactattt gcaacagtgc
     6661 cgttgatcgt gctatgatcg actgatgtca tcagcggtgg agtgcaatgt cgtgcaatac
     6721 gaatggcgaa aagccgagct catcggtcag cttctcaacc ttggggttac ccccggcggt
     6781 gtgctgctgg tccacagctc cttccgtagc gtccggcccc tcgaagatgg gccacttgga
     6841 ctgatcgagg ccctgcgtgc tgcgctgggt ccgggaggga cgctcgtcat gccctcgtgg
     6901 tcaggtctgg acgacgagcc gttcgatcct gccacgtcgc ccgttacacc ggaccttgga
     6961 gttgtctctg acacattctg gcgcctgcca aatgtaaagc gcagcgccca tccatttgcc
     7021 tttgcggcag cggggccaca ggcagagcag atcatctctg atccattgcc cctgccacct
     7081 cactcgcctg caagcccggt cgcccgtgtc catgaactcg atgggcaggt acttctcctc
     7141 ggcgtgggac acgatgccaa cacgacgctg catcttgccg agttgatggc aaaggttccc
     7201 tatggggtgc cgagacactg caccattctt caggatggca agttggtacg cgtcgattat
     7261 ctcgagaatg accactgctg tgagcgcttt gccttggcgg acaggtggct caaggagaag
     7321 agccttcaga aggaaggtcc agtcggtcat gcctttgctc ggttgatccg ctcccgcgac
     7381 attgtggcga cagccctggg tcaactgggc cgagatccgt tgatcttcct gcatccgcca
     7441 gaggcgggat gcgaagaatg cgatgccgct cgccagtcga ttggctgact gtcagaccaa
     7501 gtttactcat atatacttta gattgatttc tgaaagcgac caggtgctcg gcgtggcaag
     7561 actcgcagcg aacccgtaga aagccatgct ccagccgccc gcattggaga aattcttcaa
     7621 attcccgttg cacatagccc ggcaattcct ttccctgctc tgccataagc gcagcgaatg
     7681 ccgggtaata ctcgtcaacg atctgataga gaagggtttg ctcgggtcgg tggctctggt
     7741 aacgaccagt atcccgatcc cggctggccg tcctggccgc cacatgaggc atgttccgcg
     7801 tccttgcaat actgtgttta catacagtct atcgcttagc ggaaagttct tttaccctca
     7861 gccgaaatgc ctgccgttgc tagacattgc cagccagtgc ccgtcactcc cgtactaact
     7921 gtcacgaacc cctgcaataa ctgtcacgcc cccctgcaat aactgtcacg aacccctgca
     7981 ataactgtca cgcccccaaa cctgcaaacc cagcaggggc gggggctggc ggggtgttgg
     8041 aaaaatccat ccatgattat ctaagaataa tccactaggc gcggttatca gcgcccttgt
     8101 ggggcgctgc tgcccttgcc caatatgccc ggccagaggc cggatagctg gtctattcgc
     8161 tgcgctaggc tacacaccgc cccaccgctg cgcggcaggg ggaaaggcgg gcaaagcccg
     8221 ctaaacccca caccaaaccc cgcagaaata cgctggagcg cttttagccg ctttagcggc
     8281 ctttccccct acccgaaggg tgggggcgcg tgtgcagccc cgcagggcct gtctcggtcg
     8341 atcattcagc ccggctcatc cttctggcgt ggcggcagac cgaacaaggc gcggtcgtgg
     8401 tcgcgttcaa ggtacgcatc cattgccgcc atgagccgat cctccggcca ctcgctgctg
     8461 ttcaccttgg ccaaaatcat ggcccccacc agcaccttgc gccttgtttc gttcttgcgc
     8521 tcttgctgct gttcccttgc ccgcacccgc tgaatttcgg cattgattcg cgctcgttgt
     8581 tcttcgagct tggccagccg atccgccgcc ttgttgctcc ccttaaccat cttgacaccc
     8641 cattgttaat gtgctgtctc gtaggctatc atggaggcac agcggcggca atcccgaccc
     8701 tactttgtag gggagggcgc acttaccggt ttctcttcga gaaactggcc taacggccac
     8761 ccttcgggcg gtgcgctctc cgagggccat tgcatggagc cgaaaagcaa aagcaacagc
     8821 gaggcagcat ggcgatttat caccttacgg cgaaaaccgg cagcaggtcg ggcggccaat
     8881 cggccagggc caaggccgac tacatccagc gcgaaggcaa gtatgcccgc gacatggatg
     8941 aagtcttgca cgccgaatcc gggcacatgc cggagttcgt cgagcggccc gccgactact
     9001 gggatgctgc cgacctgtat gaacgcgcca atgggcggct gttcaaggag gtcgaatttg
     9061 ccctgccggt cgagctgacc ctcgaccagc agaaggcgct ggcgtccgag ttcgcccagc
     9121 acctgaccgg tgccgagcgc ctgccgtata cgctggccat ccatgccggt ggcggcgaga
     9181 acccgcactg ccacctgatg atctccgagc ggatcaatga cggcatcgag cggcccgccg
     9241 ctcagtggtt caagcggtac aacggcaaga ccccggagaa gggcggggca cagaagaccg
     9301 aagcgctcaa gcccaaggca tggcttgagc agacccgcga ggcatgggcc gaccatgcca
     9361 accgggcatt agagcgggct ggccacgacg cccgcattga ccacagaaca cttgaggcgc
     9421 agggcatcga gcgcctgccc ggtgttcacc tggggccgaa cgtggtggag atggaaggcc
     9481 ggggcatccg caccgaccgg gcagacgtgg ccctgaacat cgacaccgcc aacgcccaga
     9541 tcatcgactt acaggaatac cgggaggcaa tagaccatga acgcaatcga cagagtgaag
     9601 aaatccagag gcatcaacga gttagcggag cagatcgaac cgctggccca gagcatggcg
     9661 acactggccg acgaagcccg gcaggtcatg agccagaccc agcaggccag cgaggcgcag
     9721 gcggcggagt ggctgaaagc ccagcgccag acaggggcgg catgggtgga gctggccaaa
     9781 gagttgcggg aggtagccgc cgaggtgagc agcgccgcgc agagcgcccg gagcgcgtcg
     9841 cgggggtggc actggaagct atggctaacc gtgatgctgg cttccatgat gcctacggtg
     9901 gtgctgctga tcgcatcgtt gctcttgctc gacctgacgc cactgacaac cgaggacggc
     9961 tcgatctggc tgcgcttggt ggcccgatga agaacgacag gactttgcag gccataggcc
    10021 gacagctcaa ggccatgggc tgtgagcgct tcgatatcgg cgtcagggac gccaccaccg
    10081 gccagatgat gaaccgggaa tggtcagccg ccgaagtgct ccagaacacg ccatggctca
    10141 agcggatgaa tgcccagggc aatgacgtgt atatcaggcc cgccgagcag gagcggcatg
    10201 gtctggtgct ggtggacgac ctcagcgagt ttgacctgga tgacatgaaa gccgagggcc
    10261 gggagcctgc cctggtagtg gaaaccagcc cgaagaacta tcaggcatgg gtcaaggtgg
    10321 ccgacgccgc aggcggtgaa cttcgggggc agattgcccg gacgctggcc agcgagtacg
    10381 acgccgaccc ggccagcgcc gacagccgcc actatggccg cttggcgggc ttcaccaacc
    10441 gcaaggacaa gcacaccacc cgcgccggtt atcagccgtg ggtgctgctg cgtgaatcca
    10501 agggcaagac cgccaccgct ggcccggcgc tggtgcagca ggctggccag cagatcgagc
    10561 aggcccagcg gcagcaggag aaggcccgca ggctggccag cctcgaactg cccgagcggc
    10621 agcttagccg ccaccggcgc acggcgctgg acgagtaccg cagcgagatg gccgggctgg
    10681 tcaagcgctt cggtgatgac ctcagcaagt gcgactttat cgccgcgcag aagctggcca
    10741 gccggggccg cagtgccgag gaaatcggca aggccatggc cgaggccagc ccagcgctgg
    10801 cagagcgcaa gcccggccac gaagcggatt acatcgagcg caccgtcagc aaggtcatgg
    10861 gtctgcccag cgtccagctt gcgcgggccg agctggcacg ggcaccggca ccccgccagc
    10921 gaggcatgga caggggcggg ccagatttca gcatgtagtg cttgcgttgg tactcacgcc
    10981 tgttatacta tgagtactca cgcacagaag ggggttttat ggaatacgaa aaaagcgctt
    11041 cagggtcggt ctacctgatc aaaagtgaca agggctattg gttgcccggt ggctttggtt
    11101 atacgtcaaa caaggccgag gctggccgct tttcagtcgc tgatatggcc agccttaacc
    11161 ttgacggctg caccttgtcc ttgttccgcg aagacaagcc tttcggcccc ggcaagtttc
    11221 tcggtgactg atatgaaaga ccaaaaggac aagcagaccg gcgacctgct ggccagccct
    11281 gacgctgtac gccaagcgcg atatgccgag cgcatgaagg ccaaagggat gcgtcagcgc
    11341 aagttctggc tgaccgacga cgaatacgag gcgctgcgcg agtgcctgga agaactcaga
    11401 gcggcgcagg gcgggggtag tgaccccgcc agcgcctaac caccaactgc ctgcaaagga
    11461 ggcaatcaat ggctacccat aagcctatca atattctgga ggcgttcgca gcagcgccgc
    11521 caccgctgga ctacgttttg cccaacatgg tggccggtac ggtcggggcg ctggtgtcgc
    11581 ccggtggtgc cggtaaatcc atgctggccc tgcaactggc cgcacagatt gcaggcgggc
    11641 cggatctgct ggaggtgggc gaactgccca ccggcccggt gatctacctg cccgccgaag
    11701 acccgcccac cgccattcat caccgcctgc acgcccttgg ggcgcacctc agcgccgagg
    11761 aacggcaagc cgtggctgac ggcctgctga tccagccgct gatcggcagc ctgcccaaca
    11821 tcatggcccc ggagtggttc gacggcctca agcgcgccgc cgagggccgc cgcctgatgg
    11881 tgctggacac gctgcgccgg ttccacatcg aggaagaaaa cgccagcggc cccatggccc
    11941 aggtcatcgg tcgcatggag gccatcgccg ccgataccgg gtgctctatc gtgttcctgc
    12001 accatgccag caagggcgcg gccatgatgg gcgcaggcga ccagcagcag gccagccggg
    12061 gcagctcggt actggtcgat aacatccgct ggcagtccta cctgtcgagc atgaccagcg
    12121 ccgaggccga ggaatggggt gtggacgacg accagcgccg gttcttcgtc cgcttcggtg
    12181 tgagcaaggc caactatggc gcaccgttcg ctgatcggtg gttcaggcgg catgacggcg
    12241 gggtgctcaa gcccgccgtg ctggagaggc agcgcaagag caagggggtg ccccgtggtg
    12301 aagcctaaga acaagcacag cctcagccac gtccggcacg acccggcgca ctgtctggcc
    12361 cccggcctgt tccgtgccct caagcggggc gagcgcaagc gcagcaagct ggacgtgacg
    12421 tatgactacg gcgacggcaa gcggatcgag ttcagcggcc cggagccgct gggcgctgat
    12481 gatctgcgca tcctgcaagg gctggtggcc atggctgggc ctaatggcct agtgcttggc
    12541 ccggaaccca agaccgaagg cggacggcag ctccggctgt tcctggaacc caagtgggag
    12601 gccgtcaccg ctgatgccat ggtggtcaaa ggtagctatc gggcgctggc aaaggaaatc
    12661 ggggcagagg tcgatagtgg tggggcgctc aagcacatac aggactgcat cgagcgcctt
    12721 tggaaggtat ccatcatcgc ccagaatggc cgcaagcggc aggggtttcg gctgctgtcg
    12781 gagtacgcca gcgacgaggc ggacgggcgc ctgtacgtgg ccctgaaccc cttgatcgcg
    12841 caggccgtca tgggtggcgg ccagcatgtg cgcatcagca tggacgaggt gcgggcgctg
    12901 gacagcgaaa ccgcccgcct gctgcaccag cggctgtgtg gctggatcga ccccggcaaa
    12961 accggcaagg cttccataga taccttgtgc ggctatgtct ggccgtcaga ggccagtggt
    13021 tcgaccatgc gcaagcgccg ccagcgggtg cgcgaggcgt tgccggagct ggtcgcgctg
    13081 ggctggacgg taaccgagtt cgcggcgggc aagtacgaca tcacccgacc caaggcggca
    13141 ggctgacccc ccccactcta ttgtaaacaa gacattttta tcttttatat tcaatggctt
    13201 attttcctgc taattggtaa taccatgaaa aataccatgc tcagaaaagg cttaacaata
    13261 ttttgaaaaa ttgcctactg agcgctgccg cacagctcca taggccgctt tcctggcttt
    13321 gcttccagat gtatgctctt ctgctcccga acgccagcaa gacgtagccc agcgcgtcgg
    13381 ccagcttgca attcgcgcta acttacatta attgcgttgc gctcactgcc cgctttccag
    13441 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
    13501 ttgcgtattg ggcgccaggg tggtttttct tttcaccagt gagacgggca acagctgatt
    13561 gcccttcacc gcctggccct gagagagttg cagcaagcgg tccacgctgg tttgccccag
    13621 caggcgaaaa tcctgtttga tggtggttaa cggcgggata taacatgagc tgtcttcggt
    13681 atcgtcgtat cccactaccg agatatccgc accaacgcgc agcccggact cggtaatggc
    13741 gcgcattgcg cccagcgcca tctgatcgtt ggcaaccagc atcgcagtgg gaacgatgcc
    13801 ctcattcagc atttgcatgg tttgttgaaa accggacatg gcactccagt cgccttcccg
    13861 ttccgctatc ggctgaattt gattgcgagt gagatattta tgccagccag ccagacgcag
    13921 acgcgccgag acagaactta atgggcccgc taacagcgcg atttgctggt gacccaatgc
    13981 gaccagatgc tccacgccca gtcgcgtacc gtcttcatgg gagaaaataa tactgttgat
    14041 gggtgtctgg tcagagacat caagaaataa cgccggaaca ttagtgcagg cagcttccac
    14101 agcaatggca tcctggtcat ccagcggata gttaatgatc agcccactga cgcgttgcgc
    14161 gagaagattg tgcaccgccg ctttacaggc ttcgacgccg cttcgttcta ccatcgacac
    14221 caccacgctg gcacccagtt gatcggcgcg agatttaatc gccgcgacaa tttgcgacgg
    14281 cgcgtgcagg gccagactgg aggtggcaac gccaatcagc aacgactgtt tgcccgccag
    14341 ttgttgtgcc acgcggttgg gaatgtaatt cagctccgcc atcgccgctt ccactttttc
    14401 ccgcgttttc gcagaaacgt ggctggcctg gttcaccacg cgggaaacgg tctgataaga
    14461 gacaccggca tactctgcga catcgtataa cgttactggt ttcacattca ccaccctgaa
    14521 ttgactctct tccgggcgct atcatgccat accgcgaaag gttttgcacc attcgatggt
    14581 gtcaacgtaa atgccgcttc gccttcgcgc gcgaattgca agctgatccg ggcttatcga
    14641 ctgcacggtg caccaatgct tctggcgtca ggcagccatc ggaagctgtg gtatggctgt
    14701 gcaggtcgta aatcactgca taattcgtgt cgctcaaggc gcactcccgt tctggataat
    14761 gttttttgcg ccgacatcat aacggttctg gcaaatattc tgaaatgagc tgttgacaat
    14821 taatcatcgg ctcgtataat gtgtggaatt gtgagcggat aacaatttca cacaggaaac
    14881 agaattcgag ctcggtacca ggaggaaacg atgaatgcgc cagtaaatgg aacacttatt
    14941 actacacaga ttgcaaacgt tgctgaaact cttggcttgg ttaatgttaa tccacaagag
    15001 ttaaaggaaa cactgattca aacagctttc cgtactgaaa cacctgcaac tgatgcacaa
    15061 atggcttctc ttttgattgt tgctggtcaa tacaagctga acccatggac caaagagatt
    15121 tacgctttcc cagataaaaa caaagggatt attccagttg ttggcgtgga tggttggtct
    15181 cgaatcatta atggaaactc taatttcaat ggtatggaat ttaagttttc tgaaaatatg
    15241 gttcagatgg aaggcgcgaa agttgctgca cctgaatggg ttgaatgcat tatctatcgt
    15301 aaagatcgtg accaccctac tgttgttcgc gagtatttag cagagtgtta tcgtgcacca
    15361 tttaagtcaa aaactggata tgtagttgaa ggaccatggc agagtcaccc ttctcgcttc
    15421 ttgcgccaca aagcaactat tcagtgtgca cgcttagcct ttggttttgt gggtattcat
    15481 gatcaggatg aagcagaacg tatcgctgaa agtggacaag ctattaagga tgtgactagt
    15541 gaagtgccag aaggctacca agcctttgaa gatgagcatt tgcctacact caaatcagaa
    15601 gctcaatacg gcactgaacg cttgcaagct gcttatgtgg caattccaaa gggaaatctt
    15661 aaaaagcacc tttgggaagt tcactcaatt agcttaaaaa aaattgctca gtttgcagac
    15721 caagctttac agcgccaagg agaaacatat gaacattctc cagcgtagtg aagattggca
    15781 ttcggaacgc tgtgggaaag tcacagcaag ccgtgtaaag gatttaaatg caaagctcaa
    15841 taaaggaaaa gctttaaatg cattgggttt aactattcta gctgagcgcc tcactggcgt
    15901 tcagaaggaa atcttcacta accaagctat gcaatggggt atcgataacg agcctcatgc
    15961 aatagcagct tatgaaaatg aaacgggtaa ctttgtagta ggtacaggtt taattgacca
    16021 tcctttcatt gaaatgttcg gggcttcacc agatgggctt gtgggcgaca atgggcaaat
    16081 agaagttaag tgcccagata ctacaacaca cttgaacacc ctgctgacca agcaagtacc
    16141 agatgagtac attcctcaaa taactagtca gttggcttgt actcgtcgtg aatggtgtga
    16201 ctttgtgagt tatgacccac gtctaccaga aggattacag atcatcatta ttcgtgtcta
    16261 tgccaaagac ctagcaatag aagcacttga gcaagatgtt cgtaagttca acaaagctat
    16321 agatgacgca attaaaacat tgaaggtggc agcatgagtc gacctgcagg catgca
//
