LOCUS       sequence-432189-       11205 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Gal4 inducible BFP, with hygromycin resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11205)
  TITLE     Engineering Programmable Material-To-Cell Pathways Via Synthetic 
            Notch Receptors To Spatially Control Cellular Phenotypes In 
            Multi-Cellular Constructs
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11205)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pHR_5xUAS-BFP-WPRE_PGK-HygR-bGHpolyA
FEATURES             Location/Qualifiers
     source          1..11205
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    886..1110
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     LTR             1210..1443
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(1468..1486)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1469..1486)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(1776..1795)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(2009..2028)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2128..2150
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(2188..2206)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2274..2378
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2379..2447,2448..3239)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2597..2616)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3410..3998
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3899..3918
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4152..4169
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(4239..4259)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4244..4573
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4424..4559
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          5707..5772
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             5902..5922
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    6347..6481
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6384..6403)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6438..6457
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             6649..7282
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    7329..7454
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7951..8184
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8369..8413
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8698..8815
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     primer_bind     9041..9065
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             9156..9857
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     misc_feature    9893..10481
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9946..9966)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(10364..10375)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        10543..11042
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(10578..10601)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     10975..10994
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             join(11059..11205,1..879)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             join(11059..11205,1..879)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
ORIGIN
        1 gtaaatagct gcgccgatgg tttctacaaa gatcgttatg tttatcggca ctttgcatcg
       61 gccgcgctcc cgattccgga agtgcttgac attggggaat tcagcgagag cctgacctat
      121 tgcatctccc gccgtgcaca gggtgtcacg ttgcaagacc tgcctgaaac cgaactgccc
      181 gctgttctgc agccggtcgc ggaggccatg gatgcgatcg ctgcggccga tcttagccag
      241 acgagcgggt tcggcccatt cggaccgcaa ggaatcggtc aatacactac atggcgtgat
      301 ttcatatgcg cgattgctga tccccatgtg tatcactggc aaactgtgat ggacgacacc
      361 gtcagtgcgt ccgtcgcgca ggctctcgat gagctgatgc tttgggccga ggactgcccc
      421 gaagtccggc acctcgtgca cgcggatttc ggctccaaca atgtcctgac ggacaatggc
      481 cgcataacag cggtcattga ctggagcgag gcgatgttcg gggattccca atacgaggtc
      541 gccaacatct tcttctggag gccgtggttg gcttgtatgg agcagcagac gcgctacttc
      601 gagcggaggc atccggagct tgcaggatcg ccgcggctcc gggcgtatat gctccgcatt
      661 ggtcttgacc aactctatca gagcttggtt gacggcaatt tcgatgatgc agcttgggcg
      721 cagggtcgat gcgacgcaat cgtccgatcc ggagccggga ctgtcgggcg tacacaaatc
      781 gcccgcagaa gcgcggccgt ctggaccgat ggctgtgtag aagtactcgc cgatagtgga
      841 aaccgacgcc ccagcactcg tccgagggca aaggaatagg tcgagctgtg ccttctagtt
      901 gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc
      961 ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt
     1021 ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca
     1081 ggcatgctgg ggatgcggtg ggctctatgg tcgacctcga gggaattaat tcgagctcgg
     1141 tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa
     1201 aggggggact ggaagggcta attcactccc aacgaagaca agatctgctt tttgcttgta
     1261 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
     1321 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
     1381 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
     1441 gcagcatcta gaattaattc cgtgtattct atagtgtcac ctaaatcgta tgtgtatgat
     1501 acataaggtt atgtattaat tgtagccgcg ttctaacgac aatatgtaca agcctaattg
     1561 tgtagcatct ggcttactga agcagaccct atcatctctc tcgtaaactg ccgtcagagt
     1621 cggtttggtt ggacgaacct tctgagtttc tggtaacgcc gtcccgcacc cggaaatggt
     1681 cagcgaacca atcagcaggg tcatcgctag ccagatcctc tacgccggac gcatcgtggc
     1741 cggcatcacc ggcgccacag gtgcggttgc tggcgcctat atcgccgaca tcaccgatgg
     1801 ggaagatcgg gctcgccact tcgggctcat gagcgcttgt ttcggcgtgg gtatggtggc
     1861 aggccccgtg gccgggggac tgttgggcgc catctccttg catgcaccat tccttgcggc
     1921 ggcggtgctc aacggcctca acctactact gggctgcttc ctaatgcagg agtcgcataa
     1981 gggagagcgt cgaatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc
     2041 agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat
     2101 ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt
     2161 catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg cctattttta taggttaatg
     2221 tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa
     2281 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     2341 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     2401 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     2461 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     2521 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     2581 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     2641 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     2701 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     2761 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     2821 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     2881 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     2941 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     3001 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     3061 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     3121 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     3181 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     3241 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     3301 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     3361 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     3421 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     3481 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     3541 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     3601 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     3661 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     3721 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     3781 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     3841 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     3901 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     3961 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     4021 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     4081 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     4141 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
     4201 ctctccccgc gcgttggccg attcattaat gcagctgtgg aatgtgtgtc agttagggtg
     4261 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     4321 agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     4381 tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc
     4441 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
     4501 cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
     4561 aggcttttgc aaaaagcttg gacacaagac aggcttgcga gatatgtttg agaataccac
     4621 tttatcccgc gtcagggaga ggcagtgcgt aaaaagacgc ggactcatgt gaaatactgg
     4681 tttttagtgc gccagatctc tataatctcg cgcaacctat tttcccctcg aacacttttt
     4741 aagccgtaga taaacaggct gggacacttc acatgagcga aaaatacatc gtcacctggg
     4801 acatgttgca gatccatgca cgtaaactcg caagccgact gatgccttct gaacaatgga
     4861 aaggcattat tgccgtaagc cgtggcggtc tgtaccgggt gcgttactgg cgcgtgaact
     4921 gggtattcgt catgtcgata ccgtttgtat ttccagctac gatcacgaca accagcgcga
     4981 gcttaaagtg ctgaaacgcg cagaaggcga tggcgaaggc ttcatcgtta ttgatgacct
     5041 ggtggatacc ggtggtactg cggttgcgat tcgtgaaatg tatccaaaag cgcactttgt
     5101 caccatcttc gcaaaaccgg ctggtcgtcc gctggttgat gactatgttg ttgatatccc
     5161 gcaagatacc tggattgaac agccgtggga tatgggcgtc gtattcgtcc cgccaatctc
     5221 cggtcgctaa tcttttcaac gcctggcact gccgggcgtt gttcttttta acttcaggcg
     5281 ggttacaata gtttccagta agtattctgg aggctgcatc catgacacag gcaaacctga
     5341 gcgaaaccct gttcaaaccc cgctttaaac atcctgaaac ctcgacgcta gtccgccgct
     5401 ttaatcacgg cgcacaaccg cctgtgcagt cggcccttga tggtaaaacc atccctcact
     5461 ggtatcgcat gattaaccgt ctgatgtgga tctggcgcgg cattgaccca cgcgaaatcc
     5521 tcgacgtcca ggcacgtatt gtgatgagcg atgccgaacg taccgacgat gatttatacg
     5581 atacggtgat tggctaccgt ggcggcaact ggatttatga gtgggccccg gatctttgtg
     5641 aaggaacctt acttctgtgg tgtgacataa ttggacaaac tacctacaga gatttaaagc
     5701 tctaaggtaa atataaaatt tttaagtgta taatgtgtta aactactgat tctaattgtt
     5761 tgtgtatttt agattccaac ctatggaact gatgaatggg agcagtggtg gaatgccttt
     5821 aatgaggaaa acctgttttg ctcagaagaa atgccatcta gtgatgatga ggctactgct
     5881 gactctcaac attctactcc tccaaaaaag aagagaaagg tagaagaccc caaggacttt
     5941 ccttcagaat tgctaagttt tttgagtcat gctgtgttta gtaatagaac tcttgcttgc
     6001 tttgctattt acaccacaaa ggaaaaagct gcactgctat acaagaaaat tatggaaaaa
     6061 tattctgtaa cctttataag taggcataac agttataatc ataacatact gttttttctt
     6121 actccacaca ggcatagagt gtctgctatt aataactatg ctcaaaaatt gtgtaccttt
     6181 agctttttaa tttgtaaagg ggttaataag gaatatttga tgtatagtgc cttgactaga
     6241 gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca
     6301 cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc
     6361 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     6421 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat
     6481 caactggata actcaagcta accaaaatca tcccaaactt cccaccccat accctattac
     6541 cactgccaat tacctgtggt ttcatttact ctaaacctgt gattcctctg aattattttc
     6601 attttaaaga aattgtattt gttaaatatg tactacaaac ttagtagttg gaagggctaa
     6661 ttcactccca aagaagacaa gatatccttg atctgtggat ctaccacaca caaggctact
     6721 tccctgatta gcagaactac acaccagggc caggggtcag atatccactg acctttggat
     6781 ggtgctacaa gctagtacca gttgagccag ataaggtaga agaggccaat aaaggagaga
     6841 acaccagctt gttacaccct gtgagcctgc atgggatgga tgacccggag agagaagtgt
     6901 tagagtggag gtttgacagc cgcctagcat ttcatcacgt ggcccgagag ctgcatccgg
     6961 agtacttcaa gaactgctga tatcgagctt gctacaaggg actttccgct ggggactttc
     7021 cagggaggcg tggcctgggc gggactgggg agtggcgagc cctcagatcc tgcatataag
     7081 cagctgcttt ttgcctgtac tgggtctctc tggttagacc agatctgagc ctgggagctc
     7141 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
     7201 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
     7261 tcagtgtgga aaatctctag cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac
     7321 cagaggagct ctctcgacgc aggactcggc ttgctgaagc gcgcacggca agaggcgagg
     7381 ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg aggctagaag gagagagatg
     7441 ggtgcgagag cgtcagtatt aagcggggga gaattagatc gcgatgggaa aaaattcggt
     7501 taaggccagg gggaaagaaa aaatataaat taaaacatat agtatgggca agcagggagc
     7561 tagaacgatt cgcagttaat cctggcctgt tagaaacatc agaaggctgt agacaaatac
     7621 tgggacagct acaaccatcc cttcagacag gatcagaaga acttagatca ttatataata
     7681 cagtagcaac cctctattgt gtgcatcaaa ggatagagat aaaagacacc aaggaagctt
     7741 tagacaagat agaggaagag caaaacaaaa gtaagaccac cgcacagcaa gcggccggcc
     7801 gctgatcttc agacctggag gaggagatat gagggacaat tggagaagtg aattatataa
     7861 atataaagta gtaaaaattg aaccattagg agtagcaccc accaaggcaa agagaagagt
     7921 ggtgcagaga gaaaaaagag cagtgggaat aggagctttg ttccttgggt tcttgggagc
     7981 agcaggaagc actatgggcg cagcgtcaat gacgctgacg gtacaggcca gacaattatt
     8041 gtctggtata gtgcagcagc agaacaattt gctgagggct attgaggcgc aacagcatct
     8101 gttgcaactc acagtctggg gcatcaagca gctccaggca agaatcctgg ctgtggaaag
     8161 atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac tcatttgcac
     8221 cactgctgtg ccttggaatg ctagttggag taataaatct ctggaacaga tttggaatca
     8281 cacgacctgg atggagtggg acagagaaat taacaattac acaagcttaa tacactcctt
     8341 aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg aattagataa
     8401 atgggcaagt ttgtggaatt ggtttaacat aacaaattgg ctgtggtata taaaattatt
     8461 cataatgata gtaggaggct tggtaggttt aagaatagtt tttgctgtac tttctatagt
     8521 gaatagagtt aggcagggat attcaccatt atcgtttcag acccacctcc caaccccgag
     8581 gggacccgac aggcccgaag gaatagaaga agaaggtgga gagagagaca gagacagatc
     8641 cattcgatta gtgaacggat ctcgacggta tcgccaaatg gcagtattca tccacaattt
     8701 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
     8761 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttcgggt
     8821 ttattacagg gacagcagag atccagtttg gatcgataag cttgatatcg aattcggagc
     8881 actgtcctcc gaacgtcgga gcactgtcct ccgaacgtcg gagcactgtc ctccgaacgt
     8941 cggagcactg tcctccgaac ggagcatgtc ctccgaacgt cggagcactg tcctccgaac
     9001 gactagttag gcgtgtacgg tgggaggcct atataagcag agctcgttta gtgaaccgtc
     9061 agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac cgggaccgat
     9121 ccagcctctc gacattcgtt ggatccgccg ccaccatgag cgagctgatt aaggagaaca
     9181 tgcacatgaa gctgtacatg gagggcaccg tggacaacca tcacttcaag tgcacatccg
     9241 agggcgaagg caagccctac gagggcaccc agaccatgag aatcaaggtg gtcgagggcg
     9301 gccctctccc cttcgccttc gacatcctgg ctactagctt cctctacggc agcaagacct
     9361 tcatcaacca cacccagggc atccccgact tcttcaagca gtccttccct gagggcttca
     9421 catgggagag agtcaccaca tacgaagacg ggggcgtgct gaccgctacc caggacacca
     9481 gcctccagga cggctgcctc atctacaacg tcaagatcag aggggtgaac ttcacatcca
     9541 acggccctgt gatgcagaag aaaacactcg gctgggaggc cttcaccgag acgctgtacc
     9601 ccgctgacgg cggcctggaa ggcagaaacg acatggccct gaagctcgtg ggcgggagcc
     9661 atctgatcgc aaacatcaag accacatata gatccaagaa acccgctaag aacctcaaga
     9721 tgcctggcgt ctactatgtg gactacagac tggaaagaat caaggaggcc aacaacgaga
     9781 cctacgtcga gcagcacgag gtggcagtgg ccagatactg cgacctccct agcaaactgg
     9841 ggcacaagct taattaatgc aggcatgcaa gcttgatatc aagcttatcg ataatcaacc
     9901 tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac
     9961 gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt
    10021 cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt
    10081 tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg
    10141 cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac
    10201 ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac
    10261 tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt
    10321 tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc
    10381 ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg
    10441 ccctcagacg agtcggatct ccctttgggc cgcctccccg cgcggccgca actcccacct
    10501 gcaacatgcg tgactgactg aggccgcgac tctagagtcg acgggtaggg gaggcgcttt
    10561 tcccaaggca gtctggagca tgcgctttag cagccccgct gggcacttgg cgctacacaa
    10621 gtggcctctg gcctcgcaca cattccacat ccaccggtag gcgccaaccg gctccgttct
    10681 ttggtggccc cttcgcgcca ccttctactc ctcccctagt caggaagttc ccccccgccc
    10741 cgcagctcgc gtcgtgcagg acgtgacaaa tggaagtagc acgtctcact agtctcgtgc
    10801 agatggacag caccgctgag caatggaagc gggtaggcct ttggggcagc ggccaatagc
    10861 agctttgctc cttcgctttc tgggctcaga ggctgggaag gggtgggtcc gggggcgggc
    10921 tcaggggcgg gctcaggggc ggggcgggcg cccgaaggtc ctccggaggc ccggcattct
    10981 gcacgcttca aaagcgcacg tctgccgcgc tgttctcctc ttcctcatct ccgggccttt
    11041 cgacgtcgag tcgccaccat gaaaaagcct gaactcaccg cgacgtctgt cgagaagttt
    11101 ctgatcgaaa agttcgacag cgtctccgac ctgatgcagc tctcggaggg cgaagaatct
    11161 cgtgctttca gcttcgatgt aggagggcgt ggatatgtcc tgcgg
//
