LOCUS       sequence-432195-       10825 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This plasmid contains luciferase under doxycycline inducible 
            control..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10825)
  AUTHORS   Pan X, Heacock ML, Abdulaziz EN, Violante S, Zuckerman AL, Shrestha 
            N, Yao C, Goodman RP, Cross JR, Cracan V
  TITLE     A genetically encoded tool to increase cellular NADH/NAD(+) ratio in
            living cells.
  JOURNAL   Nat Chem Biol. 2023 Oct 26. doi: 10.1038/s41589-023-01460-w.
  PUBMED    37884806
REFERENCE   2  (bases 1 to 10825)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2023 Oct 26. doi: 10.1038/s41589-023-01460-w."
COMMENT     Sequence Label: pLVX-TetOne-Puro-LUC
FEATURES             Location/Qualifiers
     source          1..10825
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    58..173
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     CDS             complement(436..480)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    665..898
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    1395..1520
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             1567..2200
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     polyA_signal    2368..2502
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        2550..2654
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2655..2723,2724..3515)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2873..2892)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3686..4274
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4175..4194
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4428..4445
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4562..4583
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4598..4603,4604..4621,4622..4628)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4636..4652
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4641..4663
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4660..4676
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4660..4676
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     LTR             4804..5437
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    5644..6232
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             5750..5761
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     6159..6179
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6246..6845)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6329..6349)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     6826..6845
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(6854..7183)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6868..7003
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             complement(7194..7940)
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     promoter        complement(7959..8469)
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     complement(8022..8040)
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     promoter        8486..8853
                     /label=TRE3GS promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G, modified to eliminate
                     binding sites for endogenous mammalian transcription 
                     factors"
     protein_bind    8494..8512
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8530..8548
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8566..8584
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8602..8620
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8638..8656
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8674..8692
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8710..8728
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     CDS             8860..10512
                     /codon_start=1
                     /gene="luc"
                     /product="firefly luciferase"
                     /label=luciferase
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
     primer_bind     complement(8926..8944)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     polyA_signal    join(10705..10825,1..14)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 gcaataaaca agttaagtcg ataaactgga tctctgctgt ccctgtaata aacccgaaaa
       61 ttttgaattt ttgtaatttg tttttgtaat tctttagttt gtatgtctgt tgctattatg
      121 tctactattc tttcccctgc actgtacccc ccaatccccc cttttctttt aaaggcgata
      181 ccgtcgagat ccgttcacta atcgaatgga tctgtctctg tctctctctc caccttcttc
      241 ttctattcct tcgggcctgt cgggtcccct cggggttggg aggtgggtct gaaacgataa
      301 tggtgaatat ccctgcctaa ctctattcac tatagaaagt acagcaaaaa ctattcttaa
      361 acctaccaag cctcctacta tcattatgaa taattttata taccacagcc aatttgttat
      421 gttaaaccaa ttccacaaac ttgcccattt atctaattcc aataattctt gttcattctt
      481 ttcttgctgg ttttgcgatt cttcaattaa ggagtgtatt aagcttgtgt aattgttaat
      541 ttctctgtcc cactccatcc aggtcgtgtg attccaaatc tgttccagag atttattact
      601 ccaactagca ttccaaggca cagcagtggt gcaaatgagt tttccagagc aaccccaaat
      661 ccccaggagc tgttgatcct ttaggtatct ttccacagcc aggattcttg cctggagctg
      721 cttgatgccc cagactgtga gttgcaacag atgctgttgc gcctcaatag ccctcagcaa
      781 attgttctgc tgctgcacta taccagacaa taattgtctg gcctgtaccg tcagcgtcat
      841 tgacgctgcg cccatagtgc ttcctgctgc tcccaagaac ccaaggaaca aagctcctat
      901 tcccactgct cttttttctc tctgcaccac tcttctcttt gccttggtgg gtgctactcc
      961 taatggttca atttttacta ctttatattt atataattca cttctccaat tgtccctcat
     1021 atctcctcct ccaggtctga agatcagcgg ccggccgctt gctgtgcggt ggtcttactt
     1081 ttgttttgct cttcctctat cttgtctaaa gcttccttgg tgtcttttat ctctatcctt
     1141 tgatgcacac aatagagggt tgctactgta ttatataatg atctaagttc ttctgatcct
     1201 gtctgaaggg atggttgtag ctgtcccagt atttgtctac agccttctga tgtttctaac
     1261 aggccaggat taactgcgaa tcgttctagc tccctgcttg cccatactat atgttttaat
     1321 ttatattttt tctttccccc tggccttaac cgaatttttt cccatcgcga tctaattctc
     1381 ccccgcttaa tactgacgct ctcgcaccca tctctctcct tctagcctcc gctagtcaaa
     1441 atttttggcg tactcaccag tcgccgcccc tcgcctcttg ccgtgcgcgc ttcagcaagc
     1501 cgagtcctgc gtcgagagag ctcctctggt ttccctttcg ctttcaagtc cctgttcggg
     1561 cgccactgct agagattttc cacactgact aaaagggtct gagggatctc tagttaccag
     1621 agtcacacaa cagacgggca cacactactt gaagcactca aggcaagctt tattgaggct
     1681 taagcagtgg gttccctagt tagccagaga gctcccaggc tcagatctgg tctaaccaga
     1741 gagacccagt acaggcaaaa agcagctgct tatatgcagg atctgagggc tcgccactcc
     1801 ccagtcccgc ccaggccacg cctccctgga aagtccccag cggaaagtcc cttgtagcaa
     1861 gctcgatatc agcagttctt gaagtactcc ggatgcagct ctcgggccac gtgatgaaat
     1921 gctaggcggc tgtcaaacct ccactctaac acttctctct ccgggtcatc catcccatgc
     1981 aggctcacag ggtgtaacaa gctggtgttc tctcctttat tggcctcttc taccttatct
     2041 ggctcaactg gtactagctt gtagcaccat ccaaaggtca gtggatatct gacccctggc
     2101 cctggtgtgt agttctgcta atcagggaag tagccttgtg tgtggtagat ccacagatca
     2161 aggatatctt gtcttctttg ggagtgaatt agcccttcca actactaagt ttgtagtaca
     2221 tatttaacaa atacaatttc tttaaaatga aaataattca gaggaatcac aggtttagag
     2281 taaatgaaac cacaggtaat tggcagtggt aatagggtat ggggtgggaa gtttgggatg
     2341 attttggtta gcttgagtta tccagttgat ccagacatga taagatacat tgatgagttt
     2401 ggacaaacca caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct
     2461 attgctttat ttgtaaccat tataagctgc aataaacaag ttgatctccc gatccgtcga
     2521 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     2581 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     2641 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     2701 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     2761 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     2821 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     2881 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
     2941 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     3001 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     3061 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     3121 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     3181 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
     3241 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     3301 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     3361 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     3421 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     3481 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     3541 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     3601 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     3661 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     3721 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     3781 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     3841 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     3901 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     3961 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     4021 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     4081 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     4141 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     4201 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     4261 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     4321 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     4381 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     4441 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
     4501 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
     4561 ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc
     4621 gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg
     4681 attacgccaa gctctagcta gaggtcgacg gtaaacgcta gcaatgtcaa ggcctctcac
     4741 tctctgatat tcatttcttt gcaagttata aatactgaat aataagatga catgaactac
     4801 tactgctaga gattttccac actgactaaa agggtctgag ggatctctag ttaccagagt
     4861 cacacaacag acgggcacac actacttgaa gcactcaagg caagctttat tgaggcttaa
     4921 gcagtgggtt ccctagttag ccagagagct cccaggctca gatctggtct aaccagagag
     4981 acccagtaca ggcaaaaagc agctgcttat atgcaggatc tgagggctcg ccactcccca
     5041 gtcccgccca ggccacgcct ccctggaaag tccccagcgg aaagtccctt gtagcaagct
     5101 cgatatcagc agttcttgaa gtactccgga tgcagctctc gggccacgtg atgaaatgct
     5161 aggcggctgt caaacctcca ctctaacact tctctctccg ggtcatccat cccatgcagg
     5221 ctcacagggt gtaacaagct ggtgttctct cctttattgg cctcttctac cttatctggc
     5281 tcaactggta ctagcttgta gcaccatcca aaggtcagtg gatatctgac ccctggccct
     5341 ggtgtgtagt tctgctaatc agggaagtag ccttgtgtgt ggtagatcca cagatcaagg
     5401 atatcttgtc ttcgttggga gtgaattagc ccttccagtc ccctcttttc ttttaaaaag
     5461 tggctaagat ctacagctgc cttgtaagtc attggtctta aaggtacctg aggtgtgact
     5521 ggaaaaccca cctcctcctc ctcttgtgct tctagccagg cacaatcagc attggtagct
     5581 gctgtattgc tacttgtgat tgctccatgt ttttctaggt ctcgactgca gaattaattc
     5641 caggcgggga ggcggcccaa agggagatcc gactcgtctg agggcgaagg cgaagacgcg
     5701 gaagaggccg cagagccggc agcaggccgc gggaaggaag gtccgctgga ttgagggccg
     5761 aagggacgta gcagaaggac gtcccgcgca gaatccaggt ggcaacacag gcgagcagcc
     5821 atggaaagga cgtcagcttc cccgacaaca ccacggaatt gtcagtgccc aacagccgag
     5881 cccctgtcca gcagcgggca aggcaggcgg cgatgagttc cgccgtggca atagggaggg
     5941 ggaaagcgaa agtcccggaa aggagctgac aggtggtggc aatgccccaa ccagtggggg
     6001 ttgcgtcagc aaacacagtg cacaccacgc cacgttgcct gacaacgggc cacaactcct
     6061 cataaagaga cagcaaccag gatttataca aggaggagaa aatgaaagcc atacgggaag
     6121 caatagcatg atacaaaggc attaaagcag cgtatccaca tagcgtaaaa ggagcaacat
     6181 agttaagaat accagtcaat ctttcacaaa ttttgtaatc cagaggttga ttgttccaga
     6241 cgcgttcagg caccgggctt gcgggtcatg caccaggtgc gcggtccttc gggcacctcg
     6301 acgtcggcgg tgacggtgaa gccgagccgc tcgtagaagg ggaggttgcg gggcgcggag
     6361 gtctccagga aggcgggcac cccggcgcgc tcggccgcct ccactccggg gagcacgacg
     6421 gcgctgccca gacccttgcc ctggtggtcg ggcgagacgc cgacggtggc caggaaccac
     6481 gcgggctcct tgggccggtg cggcgccagg aggccttcca tctgttgctg cgcggccagc
     6541 cgggaaccgc tcaactcggc catgcgcggg ccgatctcgg cgaacaccgc ccccgcttcg
     6601 acgctctccg gcgtggtcca gaccgccacc gcggcgccgt cgtccgcgac ccacaccttg
     6661 ccgatgtcga gcccgacgcg cgtgaggaag agttcttgca gctcggtgac ccgctcgatg
     6721 tggcggtccg ggtcgacggt gtggcgcgtg gcggggtagt cggcgaacgc ggcggcgagg
     6781 gtgcgtacgg cccgggggac gtcgtcgcgg gtggcgaggc gcaccgtggg cttgtactcg
     6841 gtcatggtcg cgttttgcaa aagcctaggc ctccaaaaaa gcctcctcac tacttctgga
     6901 atagctcaga ggccgaggcg gcctcggcct ctgcataaat aaaaaaaatt agtcagccat
     6961 ggggcggaga atgggcggaa ctgggcggag ttaggggcgg gatgggcgga gttaggggcg
     7021 ggactatggt tgctgactaa ttgagatgca tgctttgcat acttctgcct gctggggagc
     7081 ctggggactt tccacacctg gttgctgact aattgagatg catgctttgc atacttctgc
     7141 ctgctgggga gcctggggac tttccacacc ctaactgaca cacattcgcg cgtttacccg
     7201 gggagcatgt caaggtcaaa atcgtcaaga gcgtcagcag gcagcatatc aaggtcaaag
     7261 tcgtcaaggg catcggctgg gagcatgtct aagtcaaaat cgtcaagggc gtcggtcggc
     7321 ccgccgcttt cgcactttag ctgtttctcc aggccacata tgattagttc caggccgaaa
     7381 aggaaggcag gttcggctcc ctgccggtcg aacagctcaa ttgcttgttt cagaagtggg
     7441 ggcatagaat cggtggtagg tgtctctctt tcctcttttg ctacttgatg ctcctgttcc
     7501 tccaatacgc agcccagtgt aaagtggccc acggcggaca gagcgtacag tgcgttctcc
     7561 agggagaagc cttgctgaca caggaacgcg agctgatttt ccagggtttc gtactgtttc
     7621 tctgttgggc gggtgccgag atgcacttta gccccgtcgc gatgtgagag gagagcacag
     7681 cggtatgact tggcgttgtt ccgcagaaag tcttgccatg actcgccttc cagggggcag
     7741 gagtgggtat gatgcctgtc cagcatctcg attggcaggg catcgagcag ggcccgcttg
     7801 ttcttcacgt gccagtacag ggtaggctgc tcaactccca gcttttgagc gagtttcctt
     7861 gtcgtcaggc cttcgatacc gactccattg agtaattcca gagcagagtt tatgactttg
     7921 ctcttgtcca gtctagacat ggtaattcga tgatccccct ggggagagag gtcggtgatt
     7981 cggtcaacga gggagccgac tgccgacgtg cgctccggag gcttgcagaa tgcggaacac
     8041 cgcgcgggca ggaacagggc ccacactacc gccccacacc ccgcctcccg caccgcccct
     8101 tcccggccgc tgctctcggc gcgccctgct gagcagccgc tattggccac agcccatcgc
     8161 ggtcggcgcg ctgccattgc tccctggcgc tgtccgtctg cgagggtact agtgagacgt
     8221 gcggcttccg tttgtcacgt ccggcacgcc gcgaaccgca aggaaccttc ccgacttagg
     8281 ggcggagcag gaagcgtcgc cggggggccc acaagggtag cggcgaagat ccgggtgacg
     8341 ctgcgaacgg acgtgaagaa tgtgcgagac ccagggtcgg cgccgctgcg tttcccggaa
     8401 ccacgcccag agcagccgcg tccctgcgca aacccagggc tgccttggaa aaggcgcaac
     8461 cccaaccccg tggaattatc acctcgagtt tactccctat cagtgataga gaacgtatga
     8521 agagtttact ccctatcagt gatagagaac gtatgcagac tttactccct atcagtgata
     8581 gagaacgtat aaggagttta ctccctatca gtgatagaga acgtatgacc agtttactcc
     8641 ctatcagtga tagagaacgt atctacagtt tactccctat cagtgataga gaacgtatat
     8701 ccagtttact ccctatcagt gatagagaac gtataagctt tgcttatgta aaccagggcg
     8761 cctataaaag agtgctgatt ttttgagtaa acttcaattc cacaacactt ttgtcttata
     8821 ccaactttcc gtaccacttc ctaccctcgt aaagaattca tggaagacgc caaaaacata
     8881 aagaaaggcc cggcgccatt ctatccgcta gaggatggaa ccgctggaga gcaactgcat
     8941 aaggctatga agagatacgc cctggttcct ggaacaattg cttttacaga tgcacatatc
     9001 gaggtgaaca tcacgtacgc ggaatacttc gaaatgtccg ttcggttggc agaagctatg
     9061 aaacgatatg ggctgaatac aaatcacaga atcgtcgtat gcagtgaaaa ctctcttcaa
     9121 ttctttatgc cggtgttggg cgcgttattt atcggagttg cagttgcgcc cgcgaacgac
     9181 atttataatg aacgtgaatt gctcaacagt atgaacattt cgcagcctac cgtagtgttt
     9241 gtttccaaaa aggggttgca aaaaattttg aacgtgcaaa aaaaattacc aataatccag
     9301 aaaattatta tcatggattc taaaacggat taccagggat ttcagtcgat gtacacgttc
     9361 gtcacatctc atctacctcc cggttttaat gaatacgatt ttgtaccaga gtcctttgat
     9421 cgtgacaaaa caattgcact gataatgaac tcctctggat ctactgggtt acctaagggt
     9481 gtggcccttc cgcatagaac tgcctgcgtc agattctcgc atgccagaga tcctattttt
     9541 ggcaatcaaa tcattccgga tactgcgatt ttaagtgttg ttccattcca tcacggtttt
     9601 ggaatgttta ctacactcgg atatttgata tgtggatttc gagtcgtctt aatgtataga
     9661 tttgaagaag agctgttttt acgatccctt caggattaca aaattcaaag tgcgttgcta
     9721 gtaccaaccc tattttcatt cttcgccaaa agcactctga ttgacaaata cgatttatct
     9781 aatttacacg aaattgcttc tgggggcgca cctctttcga aagaagtcgg ggaagcggtt
     9841 gcaaaacgct tccatcttcc agggatacga caaggatatg ggctcactga gactacatca
     9901 gctattctga ttacacccga gggggatgat aaaccgggcg cggtcggtaa agttgttcca
     9961 ttttttgaag cgaaggttgt ggatctggat accgggaaaa cgctgggcgt taatcagaga
    10021 ggcgaattat gtgtcagagg acctatgatt atgtccggtt atgtaaacaa tccggaagcg
    10081 accaacgcct tgattgacaa ggatggatgg ctacattctg gagacatagc ttactgggac
    10141 gaagacgaac acttcttcat agttgaccgc ttgaagtctt taattaaata caaaggatac
    10201 caggtggccc ccgctgaatt ggagtcgata ttgttacaac accccaacat cttcgacgcg
    10261 ggcgtggcag gtcttcccga cgatgacgcc ggtgaacttc ccgccgccgt tgttgttttg
    10321 gagcacggaa agacgatgac ggaaaaagag atcgtggatt acgtcgccag tcaagtaaca
    10381 accgcgaaaa agttgcgcgg aggagttgtg tttgtggacg aagtaccgaa aggtcttacc
    10441 ggaaaactcg acgcaagaaa aatcagagag atcctcataa aggccaagaa gggcggaaag
    10501 tccaaattgt aaaccggtgc cggcggatcc agaccacctc ccctgcgagc taagctggac
    10561 agccaatgac gggtaagaga gtgacatttt tcactaacct aagacaggag ggccgtcaga
    10621 gctactgcct aatccaaaga cgggtaaaag tgataaaaat gtatcactcc aacctaagac
    10681 aggcgcagct tccgagggat ttgagatcca gacatgataa gatacattga tgagtttgga
    10741 caaaccaaaa ctagaatgca gtgaaaaaaa tgccttattt gtgaaatttg tgatgctatt
    10801 gccttatttg taaccattat aagct
//
