LOCUS       sequence-432246-         571 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express the Rotavirus SA11 NSP4 fused with the PEST degradation 
            sequence from mouse ornithine decarboxylase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 571)
  TITLE     Rotavirus Nonstructural Protein (NSP) plasmids
REFERENCE   2  (bases 1 to 571)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pT7-NSP4SA11-hPEST
FEATURES             Location/Qualifiers
     source          1..571
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             179..298
                     /codon_start=1
                     /product="PEST degradation sequence from mouse ornithine 
                     decarboxylase"
                     /label=hPEST
                     /note="human codon-optimized"
                     /translation="SHGFPPEVEEQAAGTLPMSCAQESGMDRHPAACASARINV"
ORIGIN
        1 gactacacgt gaaattgaac aagtagagtt gcttaaacgc atttacgata aattgacggt
       61 gcaaacgaca ggtgaaatag atatgacaaa agagatcaat caaaaaaacg tgagaacgct
      121 agaagaatgg gaaagtggaa aaaatcctta tgaaccaaga gaagtgactg cagcaatgtc
      181 tcacggcttt ccgcctgagg ttgaagagca agccgccggt acattgccta tgtcctgcgc
      241 acaagaaagc ggtatggacc ggcacccagc cgcttgtgct tcagctcgca tcaacgtcta
      301 agaggttgag ctgccgtcga ctgtcctcgg aagcggcgga gttctttaca gtaagcacca
      361 tcggacctga tggctgactg agaagccaca gtcagccata tcgcgtgtgg ctcaagcctt
      421 aatcccgttt aaccaatccg gtcagcaccg gacgttaatg gaaggaacgg tcttaatgtg
      481 accgggtcgg catggcatct ccacctcctc gcggtccgac ctgggcatcc gaaggaggac
      541 gtcgtccact cggatggcta agggagagct c
//
