LOCUS       sequence-432376-       10371 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Full length mouse integrin b6 expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10371)
  AUTHORS   Yuxin Hao, Jiabin Yan, Courtney Fraser, Aiping Jiang, Murali 
            Anuganti, Roushu Zhang, Kenneth Lloyd, Joseph Jardine, Jessica 
            Coppola, Rob Meijers, Jing Li, Timothy A.
  TITLE     Synthetic integrin antibodies discovered by yeast display reveal 
            alpha-V subunit pairing preferences with beta subunits
  JOURNAL   bioRxiv 2024.01.26.577394
REFERENCE   2  (bases 1 to 10371)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.01.26.577394"
COMMENT     Sequence Label: mouse b6 full length
FEATURES             Location/Qualifiers
     source          1..10371
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             21..77
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             78..788
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(223..241)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     491..510
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     polyA_signal    2382..2437
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2436..2455)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     rep_origin      complement(3147..3735)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3227..3246)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(3821..4630)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     4544..4563
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     promoter        complement(4649..4696)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             complement(5122..5721)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5205..5225)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     5702..5721
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(5731..6230)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5779..5798)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     6172..6195
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     enhancer        6314..6617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          6895..7911
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     7835..7857
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     7919..7938
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
ORIGIN
        1 gtaccagtac tgggagtggg gccaccaact tttctctgct caagcaagcc ggggatgttg
       61 aagaaaatcc agggccaatg gtgagcaagg gcgaggagga taacatggcc atcatcaagg
      121 agttcatgcg cttcaaggtg cacatggagg gctccgtgaa cggccacgag ttcgagatcg
      181 agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaagctg aaggtgacca
      241 agggtggccc cctgcccttc gcctgggaca tcctgtcccc tcagttcatg tacggctcca
      301 aggcctacgt gaagcacccc gccgacatcc ccgactactt gaagctgtcc ttccccgagg
      361 gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc gtgacccagg
      421 actcctccct gcaggacggc gagttcatct acaaggtgaa gctgcgcggc accaacttcc
      481 cctccgacgg ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc tccgagcgga
      541 tgtaccccga ggacggcgcc ctgaagggcg agatcaagca gaggctgaag ctgaaggacg
      601 gcggccacta cgacgctgag gtcaagacca cctacaaggc caagaagccc gtgcagctgc
      661 ccggcgccta caacgtcaac atcaagttgg acatcacctc ccacaacgag gactacacca
      721 tcgtggaaca gtacgaacgc gccgagggcc gccactccac cggcggcatg gacgagctgt
      781 acaagtgata aagtacttga taagctcttc aggttgaggt ctctaaaagc gtcttcctgt
      841 tctcatcaca tcatatcaag gttatatacc atcaatattg ccacagatgt tacttagcct
      901 tttaatattt ctctaattta gtgtatatgc aatgatagtt ctctgatttc tgagattgag
      961 tttctcatgt gtaatgatta tttagagttt ctctttcatc tgttcaaatt tttgtctagt
     1021 tttatttttt actgatttgt aagacttctt tttataatct gcatattaca attctcttta
     1081 ctggggtgtt gcaaatattt tctgtcattc tatggcctga cttttcttaa tggtttttta
     1141 attttaaaaa taagtcttaa tattcatgca atctaattaa caatcttttc tttgtggtta
     1201 ggactttgag tcataagaaa tttttctcta cactgaagtc atgatggcat gcttctatat
     1261 tattttctaa aagatttaaa gttttgcctt ctccatttag acttataatt cactggaatt
     1321 tttttgtgtg tatggtatga catatgggtt cccttttatt ttttacatat aaatatattt
     1381 ccctgttttt ctaaaaaaga aaaagatcat cattttccca ttgtaaaatg ccatattttt
     1441 ttcataggtc acttacatat atcaatgggt ctgtttctga gctctactct attttatcag
     1501 cctcactgtc tatccccaca catctcatgc tttgctctaa atcttgatat ttagtggaac
     1561 attctttccc attttgttct acaagaatat ttttgttatt gtctttgggc tttctatata
     1621 cattttgaaa tgaggttgac aagttaataa caggcctatt gattggaaag tttgtcaacg
     1681 aattgtgggt cttttggggt ttgctgcccc ttttacgcaa tgtggatatc ctgctttaat
     1741 gcctttatat gcatgtatac aagcaaaaca ggcttttact ttctcgccaa cttacaaggc
     1801 ctttctcagt aaacagtata tgacccttta ccccgttgct cggcaacggc ctggtctgtg
     1861 ccaagtgttt gctgacgcaa cccccactgg ttggggcttg gccataggcc atcagcgcat
     1921 gcgtggaacc tttgtgtctc ctctgccgat ccatactgcg gaactcctag ccgcttgttt
     1981 tgctcgcagc aggtctggag caaacctcat cgggaccgac aattctgtcg tactctcccg
     2041 caagtataca tcgtttccat ggctgctagg ctgtgctgcc aactggatcc tgcgcgggac
     2101 gtcctttgtt tacgtcccgt cggcgctgaa tcccgcggac gacccctccc ggggccgctt
     2161 ggggctctac cgcccgcttc tccgtctgcc gtaccgtccg accacggggc gcacctctct
     2221 ttacgcggac tccccgtctg tgccttctca tctgccggac cgtgtgcact tcgcttcacc
     2281 tctgcacgtc gcatggaggc caccgtgaac gcccaccgga acctgcccaa ggtcttgcat
     2341 aagaggactc ttggactttc agcaatgtca tctcagtggc taataaagga aatttatttt
     2401 cattgcaata gtgtgttgga attttttgtg tctctcactc ggaaggacat atgggagggc
     2461 aaatcattta aaacatcaga atgagtattt ggtttagagt ttggcaacat atgcccatat
     2521 gctggctgcc atgaacaaag gttggctata aagaggtcat cagtatatga aacagccccc
     2581 tgctgtccat tccttattcc atagaaaagc cttgacttga ggttagattt tttttatatt
     2641 ttgttttgtg ttattttttt ctttaacatc cctaaaattt tccttacatg ttttactagc
     2701 cagatttttc ctcctctcct gactactccc agtcatagct gtccctcttc tcttatggag
     2761 atctttgcat ttttagacat ttagaagcct atatcttgtt acagaattgg aattacacaa
     2821 aaattctacc atattttgaa agcttaggtt gttctgaaaa aaacaatata ttgttttcct
     2881 gggtaaacta aaagtcccct cgaggaaagg cccctaaagt gaaacagtgc aaaacgttca
     2941 aaaactgtct ggcaatacaa gttccacttt gaccaaaacg gctggcagta aaagggttaa
     3001 gaagactgtc agccttgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     3061 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     3121 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     3181 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     3241 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     3301 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     3361 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     3421 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     3481 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     3541 gctacagagt tcttgaagtg gtgggctaac tacggctaca ctagaagaac agtatttggt
     3601 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     3661 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     3721 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     3781 gacgcgcgcg taactcacgt taagggattt tggtcatgag ttagaaaaac tcatcgagca
     3841 tcaaatgaaa ctgcaattta ttcatatcag gattatcaat accatatttt tgaaaaagcc
     3901 gtttctgtaa tgaaggagaa aactcaccga ggcagttcca taggatggca agatcctggt
     3961 atcggtctgc gattccgact cgtccaacat caatacaacc tattaatttc ccctcgtcaa
     4021 aaataaggtt atcaagtgag aaatcaccat gagtgacgac tgaatccggt gagaatggca
     4081 aaagtttatg catttctttc cagacttgtt caacaggcca gccattacgc tcgtcatcaa
     4141 aatcactcgc atcaaccaaa ccgttattca ttcgtgattg cgcctgagcg aggcgaaata
     4201 cgcgatcgct gttaaaagga caattacaaa caggaatcga gtgcaaccgg cgcaggaaca
     4261 ctgccagcgc atcaacaata ttttcacctg aatcaggata ttcttctaat acctggaacg
     4321 ctgtttttcc ggggatcgca gtggtgagta accatgcatc atcaggagta cggataaaat
     4381 gcttgatggt cggaagtggc ataaattccg tcagccagtt tagtctgacc atctcatctg
     4441 taacatcatt ggcaacgcta cctttgccat gtttcagaaa caactctggc gcatcgggct
     4501 tcccatacaa gcgatagatt gtcgcacctg attgcccgac attatcgcga gcccatttat
     4561 acccatataa atcagcatcc atgttggaat ttaatcgcgg cctcgacgtt tcccgttgga
     4621 tatggctcat tttttacttc ctcaccttgt cgtattatac tatgccgata tactatgccg
     4681 atgattaatt gtcgacactg cgggggctct gtgtggtaag caggtcttaa cctttttact
     4741 gccaatgacg catgggatac gtcgtggcag taaaagggct taaatgccaa cgacgcgtcc
     4801 catacgttgt tggcatttta attcttctct ctgcagcggc agcatgtgcc gccgctgcag
     4861 agagtttcta gcgatgacag cccctctggg caacgagccg ggggggctgt ctttctttat
     4921 gttttaaatg cactgacctc ccacattccc tttttagtaa aatattcaga aataatttaa
     4981 atacatcatt gcaatgaaaa taaatgtttt ttattaggca gaatccagat gctcaaggcc
     5041 cttcataata tcccccagtt tagtagttgg acttagggaa caaaggaacc tttaatagaa
     5101 attggacagc aagaaagcga gtcaggcacc gggcttgcgg gtcatgcacc aggtgcgcgg
     5161 tccttcgggc acctcgacgt cggcggtgac ggtgaagccg agccgctcgt agaaggggag
     5221 gttgcggggc gcggatgtct ccaggaaggc gggcaccccg gcgcgctcgg ccgcctccac
     5281 tccggggagc acgacggcgc tgcccagacc cttgccctgg tggtcgggcg acacgccgac
     5341 ggtggccagg aaccacgcgg gctccttggg ccggtgcggc gccaggaggc cttccatctg
     5401 ttgctgcgcg gccagccggg aaccgctcaa ctcggccatg cgcgggccga tctcggcgaa
     5461 caccgccccc gcttcgacgc tctccggcgt ggtccagacc gccaccgcgg cgccgtcgtc
     5521 cgcgacccac accttgccga tgtcgagccc gacgcgcgtg aggaagagtt cttgcagctc
     5581 ggtgacccgc tcgatgtggc ggtccggatc gacggtgtgg cgcgtggcgg ggtagtcggc
     5641 gaacgcggcg gcgagggtgc gtacggccct ggggacgtcg tcgcgggtgg cgaggcgcac
     5701 cgtgggcttg tactcggtca tggtggcgga cgaaaggccc ggagatgagg aagaggagaa
     5761 cagcgcggca gacgtgcgct tttgaagcgt gcagaatgcc gggcctccgg aggaccttcg
     5821 ggcgcccgcc ccgcccctga gcccgcccct gagcccgccc ccggacccac cccttcccag
     5881 cctctgagcc cagaaagcga aggagcaaag ctgctattgg ccgctgcccc aaaggcctac
     5941 ccgcttccat tgctcagcgg tgctgtccat ctgcacgaga ctagtgagtc gtgctacttc
     6001 catttgtcac gtcctgcacg acgcgagctg cggggcgggg gggaacttcc tgactagggg
     6061 aggagtagaa ggtggcgcga aggggccacc aaagaacgga gccggttggc gcctaccggt
     6121 ggatgtggaa tgtgtgcgag gccagaggcc acttgtgtag cgccaagtgc ccagcggggc
     6181 tgctaaagcg catgctccag actgccttgg gaaaagcgcc tcccctaccc ggtagagaaa
     6241 cttgatctgt cgccgcaatt caaacttcgt gaggctccgg tgcccgtcag tgacctgcta
     6301 tactctggag acgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
     6361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
     6421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
     6481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
     6541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
     6601 tcatcgctat taccatggtc gaggtgagcc ccacgttctg cttcactctc cccatctccc
     6661 ccccctcccc acccccaatt ttgtatttat ttatttttta attattttgt gcagcgatgg
     6721 gggcgggggg ggggggggcg cgcgccaggc ggggsggggs ggggsgrggg gsggggsggg
     6781 gsgrggsggr gaggkgcggc ggcagccaat cagagcggcg cgctccgaaa gtttcctttt
     6841 atggcgaggc ggcggcggcg gcggccctat aaaaagcgaa gcgcgcggcg ggcgggagtc
     6901 gctgcgcgct gccttcgccc cgtgccccgc tccgccgccg cctcgcgccg cccgccccgg
     6961 ctctgactga ccgcgttact cccacaggtg agcgggcggg acggcccttc tcctccgggc
     7021 tgtaattagc gcttggttta atgacggctt gtttcttttc tgtggctgcg tgaaagcctt
     7081 gaggggctcc gggagggccc tttgtgcggg gggagcggct cggggggtgc gtgcgtgtgt
     7141 gtgtgcgtgg ggagcgccgc gtgcggctcc gcgctgcccg gcggctgtga gcgctgcggg
     7201 cgcggcgcgg ggctttgtgc gctccgcagt gtgcgcgagg ggagcgcggc cgggggcggt
     7261 gccccgcggt gcgggggggg ctgcgagggg aacaaaggct gcgtgcgggg tgtgtgcgtg
     7321 ggggggtgag cagggggtgt gggcgcgtcg gtcgggctgc aaccccccct gcacccccct
     7381 ccccgagttg ctgagcacgg cccggcttcg ggtgcggggc tccgtacggg gcgtggcgcg
     7441 gggctcgccg tgccgggcgg ggggtggcgg caggtggggg tgccgggcgg ggcggggccg
     7501 ccycgggccg gggrgggctc gggggrgggg cgcggcggcc cccggagcgc cggcggctgt
     7561 cgaggcgcgg cgagccgcag ccattgcctt ttatggtaat cgtgcgagag ggcgcaggga
     7621 cttcctttgt cccaaatctg tgcggagccg aaatctggga ggcgccgccg caccccctct
     7681 agcgggcgcg gggcgaagcg gtgcggcgcc ggcaggaagg aaatgggcgg ggagggcctt
     7741 cgtgcgtcgc cgcgccgccg tccccttctc cctctccagc ctcggggctg tccgcggggg
     7801 gacggctgcc ttcggggggg acggggcagg gcggggttcg gcttctggcg tgtgaccggc
     7861 ggctctagag cctctgctaa ccatgttcat gccttcttct ttttcctaca gctcctgggc
     7921 aacgtgctgg ttattgtgct gtctcatcat tttggcaaag aattcggctt gatcgaagcc
     7981 gtctcagggg agagaccaca cccaagctgt ctagagccgc caccatgcct ttgctcctcc
     8041 tgttgccctt gctctgggct ggcgccctcg cgggctgtgc ctggggtggt gcagaaagct
     8101 gctcagactg cctgctcaca ggaccccact gcgcctggtg ctcccaggag aatttcaccc
     8161 acctgtctgg agctggcgag aggtgcgaca ccccagcgaa tcttttagcc aaaggatgtc
     8221 aactaccctt cattgaaaac cctgtctccc gcatagaagt ccttcaaaat aagcctctca
     8281 gcgtgggccg acagaagaac agttctgaca ttgttcagat tgctcctcaa agcttggttc
     8341 ttaaattgag accagggcgt gagcagactc tgcaagtgca ggtccgccaa actgaagatt
     8401 acccagtaga tctgtattac ctcatggacc tctccgcctc tatggatgac gacctcaaca
     8461 ccatcaaaga gcttggctcc cggctggcca aagagatgtc caaactaacc agcaacttta
     8521 gactgggctt tggctctttt gtggaaaagc ccgtttctcc ttttatgaaa acaaccccag
     8581 aggaaatcac caacccttgc agtagtatcc cctatttctg cttacctaca tttggattca
     8641 agcacatttt gccattaact gatgatgccg agagattcaa tgaaattgtg aggaaacaga
     8701 agatttctgc taatattgac acacctgaag gtggatttga tgcaatcatg caggctgctg
     8761 tgtgtaagga aaagattggc tggcgcaatg actcgctcca cctcctggtt tttgtgagtg
     8821 atgccgattc tcattttgga atggacagca agctggcagg cattgtcatt cccaatgatg
     8881 gactctgtca cttggaccac aggaatgaat attccatgtc aactgtcttg gaatatccaa
     8941 ctatcggcca actcattgat aaactggtac aaaacaacgt gctactgatc tttgcagtca
     9001 cccaagaaca agtccatctg tatgagaact atgcaaaact cattcctgga gcaaccgtgg
     9061 gactgcttca gaaggattct gggaacatcc ttcagctgat catctccgct tatgaagaac
     9121 tgcggtctga ggtggaactg gaagtgttag gggacacaga aggactcaac ctgtctttca
     9181 cagctctctg taacaatggt gtcctcttcc cacaccaaaa gaaatgctcc cacatgaaag
     9241 ttggagacac agcatccttc aatgtgactg tgagcgtatc caactgtgag aaaagaagca
     9301 ggaacctcat cataaagcca gtggggctgg gggacaccct agaaatactc gtcagtgcag
     9361 aatgtgactg cgactgccag agagaaatag aaactaacag ctctaagtgt cacaatggga
     9421 atggctcctt ccagtgtggg gtgtgtacct gcaaccccgg ccacatgggt cctcactgcg
     9481 agtgtggtga ggatatggtg agcacggatt cctgcaagga gtccccgggt cacccttcat
     9541 gcagtggaag gggtgactgc tattgtgggc agtgcatctg ccacttatcg ccgtacggaa
     9601 gcatctacgg accttactgc cagtgtgaca acttctcctg tctgagacac aaaggccttc
     9661 tctgtggaga taacggtgac tgtgactgtg gcgagtgtgt gtgccgggat ggctggacag
     9721 gtgaatactg taactgtaca accaacaggg actcgtgtac atctgaggat ggagtgctgt
     9781 gcagtggacg tggggactgt gtctgtggca agtgtgtctg cagaaaccct ggagcctcag
     9841 gacccacctg tgaacgctgt cctacctgtg gcgacccctg taactctaaa cggagttgca
     9901 tcgagtgcta cctgtctgca gatggccagg cccaagaaga gtgtgctgac aagtgtaaag
     9961 ccattggtgc caccatcagt gaggaagatt tttcaaagga tacttctgtc tcctgctctc
    10021 tacaaggaga aaatgaatgc cttattacat tcctaataac tacggataat gaagggaaaa
    10081 ccatcattca caacatcaat gaaaaagact gccccaaacc tccaaacatt cccatgatca
    10141 tgttgggggt gtcactggcg atcctgctca tcggagttgt gctactgtgc atttggaagc
    10201 tgctggtatc atttcatgac cggaaggaag ttgctaaatt tgaagcagaa cgctctaagg
    10261 ccaagtggca aacgggaacc aatcctctgt accgaggttc caccagcact tttaagaacg
    10321 tgacctacaa gcacagggag aagcacaaag caggcctttc ctcagatggg g
//
