LOCUS       sequence-432523-       11452 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Stable, inducible expression of 5HT2A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11452)
  AUTHORS   Zhong X, Li Q, Polacco BJ, Patil T, DiBerto JF, Vartak R, Xu J, 
            Marley A, Foussard H, Roth BL, Eckhardt M, Von Zastrow M, Krogan NJ,
            Huttenhain R
  TITLE     An automated proximity proteomics pipeline for subcellular proteome 
            and protein interaction mapping.
  JOURNAL   bioRxiv. 2023 Apr 12:2023.04.11.536358. doi: 
            10.1101/2023.04.11.536358. Preprint.
  PUBMED    37090610
REFERENCE   2  (bases 1 to 11452)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv. 
            2023 Apr 12:2023.04.11.536358. doi: 10.1101/2023.04.11.536358. 
            Preprint."
COMMENT     Sequence Label: 5HT2A-APEX2
FEATURES             Location/Qualifiers
     source          1..11452
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        593..922
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      773..908
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             931..1530
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(931..950)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     1427..1447
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    1544..2132
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1597..1617)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2015..2026)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2339..2972
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(3100..3116)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3100..3116)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3113..3135)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3124..3140
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3148..3154,3155..3172,3173..3178))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3193..3214
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3331..3348)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3502..4090)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3582..3601)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4261..5052,5053..5121))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4884..4903
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5122..5226)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    5274..5408
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     LTR             5576..6209
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    6256..6381
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6878..7111
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7296..7340
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7603..7718
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     polyA_signal    7762..7896
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        complement(10375..10742)
                     /label=TRE3GS promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G, modified to eliminate
                     binding sites for endogenous mammalian transcription 
                     factors"
     protein_bind    10500..10518
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10536..10554
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10572..10590
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10608..10626
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10644..10662
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10680..10698
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10716..10734
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        10759..11269
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     11188..11206
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             join(11288..11452,1..582)
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
ORIGIN
        1 ccaatcgaga tgctggacag gcatcatacc cactcctgcc ccctggaagg cgagtcatgg
       61 caagactttc tgcggaacaa cgccaagtca taccgctgtg ctctcctctc acatcgcgac
      121 ggggctaaag tgcatctcgg cacccgccca acagagaaac agtacgaaac cctggaaaat
      181 cagctcgcgt tcctgtgtca gcaaggcttc tccctggaga acgcactgta cgctctgtcc
      241 gccgtgggcc actttacact gggctgcgta ttggaggaac aggagcatca agtagcaaaa
      301 gaggaaagag agacacctac caccgattct atgcccccac ttctgaaaca agcaattgag
      361 ctgttcgacc ggcagggagc cgaacctgcc ttccttttcg gcctggaact aatcatatgt
      421 ggcctggaga aacagctaaa gtgcgaaagc ggcgggccga ccgacgccct tgacgatttt
      481 gacttagaca tgctcccagc cgatgccctt gacgactttg accttgatat gctgcctgct
      541 gacgctcttg acgattttga ccttgacatg ctccccgggt aaacgcgcga atgtgtgtca
      601 gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
      661 caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
      721 aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc
      781 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
      841 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt
      901 tggaggccta ggcttttgca aaacgcgacc atgaccgagt acaagcccac ggtgcgcctc
      961 gccacccgcg acgacgtccc ccgggccgta cgcaccctcg ccgccgcgtt cgccgactac
     1021 cccgccacgc gccacaccgt cgacccggac cgccacatcg agcgggtcac cgagctgcaa
     1081 gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc
     1141 gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt gttcgccgag
     1201 atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca acagatggaa
     1261 ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc
     1321 tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg agtggaggcg
     1381 gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa cctccccttc
     1441 tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc
     1501 tggtgcatga cccgcaagcc cggtgcctga acgcgtctgg aacaatcaac ctctggatta
     1561 caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg
     1621 atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc
     1681 ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca
     1741 acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac
     1801 cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact
     1861 catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc
     1921 cgtggtgttg tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg
     1981 gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc
     2041 ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac
     2101 gagtcggatc tccctttggg ccgcctcccc gcctggaatt aattctgcag tcgagaccta
     2161 gaaaaacatg gagcaatcac aagtagcaat acagcagcta ccaatgctga ttgtgcctgg
     2221 ctagaagcac aagaggagga ggaggtgggt tttccagtca cacctcaggt acctttaaga
     2281 ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa gaggggactg
     2341 gaagggctaa ttcactccca acgaagacaa gatatccttg atctgtggat ctaccacaca
     2401 caaggctact tccctgatta gcagaactac acaccagggc caggggtcag atatccactg
     2461 acctttggat ggtgctacaa gctagtacca gttgagccag ataaggtaga agaggccaat
     2521 aaaggagaga acaccagctt gttacaccct gtgagcctgc atgggatgga tgacccggag
     2581 agagaagtgt tagagtggag gtttgacagc cgcctagcat ttcatcacgt ggcccgagag
     2641 ctgcatccgg agtacttcaa gaactgctga tatcgagctt gctacaaggg actttccgct
     2701 ggggactttc cagggaggcg tggcctgggc gggactgggg agtggcgagc cctcagatcc
     2761 tgcatataag cagctgcttt ttgcctgtac tgggtctctc tggttagacc agatctgagc
     2821 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
     2881 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
     2941 acccttttag tcagtgtgga aaatctctag cagtagtagt tcatgtcatc ttattattca
     3001 gtatttataa cttgcaaaga aatgaatatc agagagtgag aggccttgac attgctagcg
     3061 tttaccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg
     3121 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
     3181 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
     3241 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
     3301 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     3361 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     3421 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     3481 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     3541 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     3601 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     3661 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     3721 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     3781 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     3841 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     3901 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
     3961 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     4021 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     4081 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     4141 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     4201 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     4261 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     4321 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     4381 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     4441 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     4501 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     4561 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     4621 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     4681 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     4741 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     4801 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     4861 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     4921 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     4981 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     5041 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     5101 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
     5161 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     5221 tccgcgcaca tttccccgaa aagtgccacc tgacgtcgac ggatcgggag atcaacttgt
     5281 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     5341 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     5401 tctggatcaa ctggataact caagctaacc aaaatcatcc caaacttccc accccatacc
     5461 ctattaccac tgccaattac ctgtggtttc atttactcta aacctgtgat tcctctgaat
     5521 tattttcatt ttaaagaaat tgtatttgtt aaatatgtac tacaaactta gtagttggaa
     5581 gggctaattc actcccaaag aagacaagat atccttgatc tgtggatcta ccacacacaa
     5641 ggctacttcc ctgattagca gaactacaca ccagggccag gggtcagata tccactgacc
     5701 tttggatggt gctacaagct agtaccagtt gagccagata aggtagaaga ggccaataaa
     5761 ggagagaaca ccagcttgtt acaccctgtg agcctgcatg ggatggatga cccggagaga
     5821 gaagtgttag agtggaggtt tgacagccgc ctagcatttc atcacgtggc ccgagagctg
     5881 catccggagt acttcaagaa ctgctgatat cgagcttgct acaagggact ttccgctggg
     5941 gactttccag ggaggcgtgg cctgggcggg actggggagt ggcgagccct cagatcctgc
     6001 atataagcag ctgctttttg cctgtactgg gtctctctgg ttagaccaga tctgagcctg
     6061 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
     6121 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
     6181 cttttagtca gtgtggaaaa tctctagcag tggcgcccga acagggactt gaaagcgaaa
     6241 gggaaaccag aggagctctc tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga
     6301 ggcgaggggc ggcgactggt gagtacgcca aaaattttga ctagcggagg ctagaaggag
     6361 agagatgggt gcgagagcgt cagtattaag cgggggagaa ttagatcgcg atgggaaaaa
     6421 attcggttaa ggccaggggg aaagaaaaaa tataaattaa aacatatagt atgggcaagc
     6481 agggagctag aacgattcgc agttaatcct ggcctgttag aaacatcaga aggctgtaga
     6541 caaatactgg gacagctaca accatccctt cagacaggat cagaagaact tagatcatta
     6601 tataatacag tagcaaccct ctattgtgtg catcaaagga tagagataaa agacaccaag
     6661 gaagctttag acaagataga ggaagagcaa aacaaaagta agaccaccgc acagcaagcg
     6721 gccggccgct gatcttcaga cctggaggag gagatatgag ggacaattgg agaagtgaat
     6781 tatataaata taaagtagta aaaattgaac cattaggagt agcacccacc aaggcaaaga
     6841 gaagagtggt gcagagagaa aaaagagcag tgggaatagg agctttgttc cttgggttct
     6901 tgggagcagc aggaagcact atgggcgcag cgtcaatgac gctgacggta caggccagac
     6961 aattattgtc tggtatagtg cagcagcaga acaatttgct gagggctatt gaggcgcaac
     7021 agcatctgtt gcaactcaca gtctggggca tcaagcagct ccaggcaaga atcctggctg
     7081 tggaaagata cctaaaggat caacagctcc tggggatttg gggttgctct ggaaaactca
     7141 tttgcaccac tgctgtgcct tggaatgcta gttggagtaa taaatctctg gaacagattt
     7201 ggaatcacac gacctggatg gagtgggaca gagaaattaa caattacaca agcttaatac
     7261 actccttaat tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa ttattggaat
     7321 tagataaatg ggcaagtttg tggaattggt ttaacataac aaattggctg tggtatataa
     7381 aattattcat aatgatagta ggaggcttgg taggtttaag aatagttttt gctgtacttt
     7441 ctatagtgaa tagagttagg cagggatatt caccattatc gtttcagacc cacctcccaa
     7501 ccccgagggg acccgacagg cccgaaggaa tagaagaaga aggtggagag agagacagag
     7561 acagatccat tcgattagtg aacggatctc gacggtatcg cctttaaaag aaaagggggg
     7621 attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga catacaaact
     7681 aaagaattac aaaaacaaat tacaaaaatt caaaattttc gggtttatta cagggacagc
     7741 agagatccag tttatcgact taacttgttt attgcagctt ataatggtta caaataaggc
     7801 aatagcatca caaatttcac aaataaggca tttttttcac tgcattctag ttttggtttg
     7861 tccaaactca tcaatgtatc ttatcatgtc tggatctcaa atccctcgga agctgcgcct
     7921 gtcttaggtt ggagtgatac atttttatca cttttacccg tctttggatt aggcagtagc
     7981 tctgacggcc ctcctgtctt aggttagtga aaaatgtcac tctcttaccc gtcattggct
     8041 gtccagctta gctcgcaggg gaggtggtct ggatccctat acgcaggaga ctttctcgtt
     8101 aacgccgtca ctattgtctt tggaggcttc ttcacttcct gagcctcccc ctgagcctcc
     8161 ggcatcggca aatcccagct cgctcagctt ctggtgggcc tcggcataat cggcgaagaa
     8221 ggcatcctcg tcggcggcgt acttatccac caggggtctg aacacggggt cggacagcag
     8281 ggccttgtcg ctaggcagct gcagcaggcc ctctttctcg ccgctcagca gctcggtgaa
     8341 gtagctgttg tcgaagatca gggggttgct ggtccagggg ccctcgaagc cgcttctctc
     8401 tttgtgggcg gctccgattg tgtggccgcc agacagagcc acgatatcct ggtcggtcag
     8461 gcccatggcc ttgccgaaca catctctcag gtggtcgctg cccttggtag gatcgggcag
     8521 tctgccctct ggaggaggct caggcttgtc ctctctgccg gggtggaagg gcaccttagg
     8581 tccgcctgtc acttccacag ccaccacgcc ggccagctgg tagaagtcgg cgtagctcag
     8641 gatggggaac tcggccttca gaggttccag cagccgcacg gcgatatcca gtccattgtt
     8701 ggcgctgtgg gccagctcgg cagggtgctt aatggtgccg aaagggccgc ctgtcttggt
     8761 gcccttatcg aaggtgccgg cgctgtgaaa ggccagtctc agcatcagag gggcgcatct
     8821 cttctcggcg ataaagcccc gcagcttctt cttggccttt tccacggcgt cctggtaatc
     8881 ggcagacacg gtagggtagc tcttgccgcc gctgcctcca cctgaaccgc cgtgctgttt
     8941 gcccagggcc accatactgc agtcgttgtc tgtggtcttg gcatcttgtt tagagttttt
     9001 tttctgcccc atctgcagtt gggagctctt atatgccagt gcgggtatag tattgaccag
     9061 tataagctgg agtggcttct tattctcttt gtactgacac tggatgtacc gggaaaaagc
     9121 ggaccgatag gttttgttaa aaagggtgta gaccaatgga ttcacagctg aggacagata
     9181 tccgatccat acaaacacgt tcaacaaagc tccgataaca tcctcgttgc aggactcttt
     9241 gcagataacc gccatgatgt tcgttataaa gaatggacac cacatcacaa caaacaggaa
     9301 aaacacaatg cccaggacct tgcaagcctt ctgctcgttg ctgatgcttt gcatggtgcg
     9361 ccggccagta taagacccgg gctctcggtg tatgctccgc tggaacagtt tctcgctgct
     9421 cagggaggat tgaggcagga agctaaaact tgcaagcttg gcccgggtgc ccaaatcgct
     9481 aacgcaaagg gtcgcttcct tttgaaggga cttaattgtc aggaagtaag tgatcaccat
     9541 aatggtaaga ggaatgaaga aggacacgaa gcttcctata agtacgaaat tgtcatcagc
     9601 cagaaggcaa ctgccctctt tgaagacctt agagtcatcc tgaaggccaa atacaggtat
     9661 cggcatgctg atgccaactg aaatggtcca gaccgcgatg attttcagaa aggctttagt
     9721 tcggctgttg aatctgctgt ggtggatggg gttttgaata gcgacgtagc gatccaggct
     9781 aatagcacaa aggtgcatta tgcttgcggt actaaagaga acgtccagat agatccagac
     9841 ggcacacaac ttggatggga gtggccagcg gtacccgtac agtatagtca acatggatac
     9901 aggcatcacc agaaacccaa gcagcatatc cgcaatcgcc agagacatca ggaagtagtt
     9961 cgtggcgttc tgcagctttt tttccaggga cacggccatg attacgagaa tattgccagc
    10021 tatagtgagt atgataacca cggcggtgag cagcgcagac cagttctttt cctgcaaatg
    10081 caggagagac aaacaacttg gggacaggca tccctcacag gacagattgg tccggttttc
    10141 actgtctact gtccaattga aggcgtctga tgtattagcc tcaccactgt tgaagtcgtt
    10201 ggagtaaagt cgggtatcat cattgagctg catgagagag ttagttgtgc tggacagtga
    10261 ggtattctcc tcgcacaaga tatccatatc gatgctggcg tcatcatcgt ccttgtagtc
    10321 ggcgaatacc aggcagaaga tgtagctcag ggcgatgatc gtcttcatga attctttacg
    10381 agggtaggaa gtggtacgga aagttggtat aagacaaaag tgttgtggaa ttgaagttta
    10441 ctcaaaaaat cagcactctt ttataggcgc cctggtttac ataagcaaag cttatacgtt
    10501 ctctatcact gatagggagt aaactggata tacgttctct atcactgata gggagtaaac
    10561 tgtagatacg ttctctatca ctgataggga gtaaactggt catacgttct ctatcactga
    10621 tagggagtaa actccttata cgttctctat cactgatagg gagtaaagtc tgcatacgtt
    10681 ctctatcact gatagggagt aaactcttca tacgttctct atcactgata gggagtaaac
    10741 tcgaggtgat aattccacgg ggttggggtt gcgccttttc caaggcagcc ctgggtttgc
    10801 gcagggacgc ggctgctctg ggcgtggttc cgggaaacgc agcggcgccg accctgggtc
    10861 tcgcacattc ttcacgtccg ttcgcagcgt cacccggatc ttcgccgcta cccttgtggg
    10921 ccccccggcg acgcttcctg ctccgcccct aagtcgggaa ggttccttgc ggttcgcggc
    10981 gtgccggacg tgacaaacgg aagccgcacg tctcactagt accctcgcag acggacagcg
    11041 ccagggagca atggcagcgc gccgaccgcg atgggctgtg gccaatagcg gctgctcagc
    11101 agggcgcgcc gagagcagcg gccgggaagg ggcggtgcgg gaggcggggt gtggggcggt
    11161 agtgtgggcc ctgttcctgc ccgcgcggtg ttccgcattc tgcaagcctc cggagcgcac
    11221 gtcggcagtc ggctccctcg ttgaccgaat caccgacctc tctccccagg gggatcatcg
    11281 aattaccatg tctagactgg acaagagcaa agtcataaac tctgctctgg aattactcaa
    11341 tggagtcggt atcgaaggcc tgacgacaag gaaactcgct caaaagctgg gagttgagca
    11401 gcctaccctg tactggcacg tgaagaacaa gcgggccctg ctcgatgccc tg
//
