LOCUS       sequence-432867-        7908 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Human immunoglobulin gamma 1 antibody expression vector (lambda 
            light chain) without variable regions. Enables Colony-PCR screening 
            of false-positive (PCR vector template) colonies .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7908)
  AUTHORS   Dodev TS, Karagiannis P, Gilbert AE, Josephs DH, Bowen H, James LK, 
            Bax HJ, Beavil R, Pang MO, Gould HJ, Karagiannis SN, Beavil AJ
  TITLE     A tool kit for rapid cloning and expression of recombinant 
            antibodies.
  JOURNAL   Sci Rep. 2014 Jul 30;4:5885. doi: 10.1038/srep05885.
  PUBMED    25073855
REFERENCE   2  (bases 1 to 7908)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2014-07-30"; volume: "4:5885. doi"; pages: " 
            10.1038/srep05885"
COMMENT     Sequence Label: pVITRO1-dV-IgG1/lambda
FEATURES             Location/Qualifiers
     source          1..7908
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        51..242
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(74..94)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        256..1562
                     /label=mEF-1-alpha promoter
                     /note="strong constitutive promoter for mouse elongation 
                     factor EF-1-alpha"
     intron          583..1530
                     /label=mEF-1-alpha intron
                     /note="intron upstream of the start codon of mouse 
                     EF-1-alpha"
     polyA_signal    2649..2770
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2660..2679)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2714..2733
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      2958..3546
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3447..3466
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        3626..3929
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4044..5349
                     /label=rEF-1-alpha promoter
                     /note="strong constitutive promoter for rat elongation 
                     factor EF-1-alpha"
     misc_feature    4386..5317
                     /label=rEF-1-alpha intron
                     /note="intron upstream of the start codon of rat 
                     EF-1-alpha"
     misc_feature    5755..6199
                     /label=FMDV IRES
                     /note="internal ribosome entry site (IRES) of the 
                     foot-and-mouth disease virus"
     promoter        6232..6278
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        6270..6288
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             6298..7323
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     polyA_signal    7335..7907
                     /label=EF-1-alpha poly(A) signal
                     /note="human EF-1-alpha polyadenylation signal"
ORIGIN
        1 cctgcagggc ctgaaataac ctctgaaaga ggaacttggt taggtacctt ctgaggcgga
       61 aagaaccagc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca
      121 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca
      181 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc
      241 ccactagtgg agccgagagt aattcataca aaaggaggga tcgccttcgc aaggggagag
      301 cccagggacc gtccctaaat tctcacagac ccaaatccct gtagccgccc cacgacagcg
      361 cgaggagcat gcgctcaggg ctgagcgcgg ggagagcaga gcacacaagc tcatagaccc
      421 tggtcgtggg ggggaggacc ggggagctgg cgcggggcaa actgggaaag cggtgtcgtg
      481 tgctggctcc gccctcttcc cgagggtggg ggagaacggt atataagtgc ggcagtcgcc
      541 ttggacgttc tttttcgcaa cgggtttgcc gtcagaacgc aggtgagggg cgggtgtggc
      601 ttccgcgggc cgccgagctg gaggtcctgc tccgagcggg ccgggccccg ctgtcgtcgg
      661 cggggattag ctgcgagcat tcccgcttcg agttgcgggc ggcgcgggag gcagagtgcg
      721 aggcctagcg gcaaccccgt agcctcgcct cgtgtccggc ttgaggccta gcgtggtgtc
      781 cgcgccgccg ccgcgtgcta ctccggccgc actctggtct tttttttttt tgttgttgtt
      841 gccctgctgc cttcgattgc cgttcagcaa taggggctaa caaagggagg gtgcggggct
      901 tgctcgcccg gagcccggag aggtcatggt tggggaggaa tggagggaca ggagtggcgg
      961 ctggggcccg cccgccttcg gagcacatgt ccgacgccac ctggatgggg cgaggcctgg
     1021 ggtttttccc gaagcaacca ggctggggtt agcgtgccga ggccatgtgg ccccagcacc
     1081 cggcacgatc tggcttggcg gcgccgcgtt gccctgcctc cctaactagg gtgaggccat
     1141 cccgtccggc accagttgcg tgcgtggaaa gatggccgct cccgggccct gttgcaagga
     1201 gctcaaaatg gaggacgcgg cagcccggtg gagcgggcgg gtgagtcacc cacacaaagg
     1261 aagagggcct ggtccctcac cggctgctgc ttcctgtgac cccgtggtcc tatcggccgc
     1321 aatagtcacc tcgggctttt gagcacggct agtcgcggcg gggggagggg atgtaatggc
     1381 gttggagttt gttcacattt ggtgggtgga gactagtcag gccagcctgg cgctggaagt
     1441 catttttgga atttgtcccc ttgagttttg agcggagcta attctcgggc ttcttagcgg
     1501 ttcaaaggta tcttttaaac ccttttttag gtgttgtgaa aaccaccgct aattcaaagc
     1561 aaatggactg gacctggagg atcctcttct tggtggcggc cgccacaggc gcgcactccg
     1621 aattcgatat cctcgaggct agcaccaagg gcccatcggt cttccccctg gcaccctcct
     1681 ccaagagcac ctctgggggc acagcggccc tgggctgcct ggtcaaggac tacttccccg
     1741 aaccggtgac ggtgtcgtgg aactcaggcg ccctgaccag cggcgtgcac accttcccgg
     1801 ctgtcctaca gtcctcagga ctctactccc tcagcagcgt ggtgaccgtg ccctccagca
     1861 gcttgggcac ccagacctac atctgcaacg tgaatcacaa gcccagcaac accaaggtgg
     1921 acaagaaagt tgagcccaaa tcttgtgaca aaactcacac atgcccaccg tgcccagcac
     1981 ctgaactcct ggggggaccg tcagtcttcc tcttcccccc aaaacccaag gacaccctca
     2041 tgatctcccg gacccctgag gtcacatgcg tggtggtgga cgtgagccac gaagaccctg
     2101 aggtcaagtt caactggtac gtggacggcg tggaggtgca taatgccaag acaaagccgc
     2161 gggaggagca gtacaacagc acgtaccgtg tggtcagcgt cctcaccgtc ctgcaccagg
     2221 actggctgaa tggcaaggag tacaagtgca aggtctccaa caaagccctc ccagccccca
     2281 tcgagaaaac catctccaaa gccaaagggc agccccgaga accacaggtg tacaccctgc
     2341 ccccatcccg ggatgagctg accaagaacc aggtcagcct gacctgcctg gtcaaaggct
     2401 tctatcccag cgacatcgcc gtggagtggg agagcaatgg gcagccggag aacaactaca
     2461 agaccacgcc tcccgtgctg gactccgacg gctccttctt cctctacagc aagctcaccg
     2521 tggacaagag caggtggcag caggggaacg tcttctcatg ctccgtgatg catgaggctc
     2581 tgcacaacca ctacacgcag aagagcctct ccctgtctcc gggtaaatga ctagctggcc
     2641 agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa
     2701 atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa
     2761 taaacaagtt aacaacacca attgcattca ttttatgttt caggttcagg gggaggtgtg
     2821 ggaggttttt taaagcaagt aaaacctcta caaatgtggt atggaaatgt taattaacta
     2881 gccatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     2941 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     3001 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     3061 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
     3121 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     3181 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     3241 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     3301 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     3361 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     3421 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     3481 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     3541 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     3601 cacatgttct taattaacct gcaggcgtta cataacttac ggtaaatggc ccgcctggct
     3661 gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc
     3721 caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg
     3781 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat
     3841 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     3901 tctacgtatt agtcatcgct attaccatga tgatgcggtt ttggcagtac atcaatgggc
     3961 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     4021 gtttgttttg actagtggag ccgagagtaa ttcatacaaa aggagggatc gccttcgcaa
     4081 ggggagagcc cagggaccgt ccctaaattc tcacagaccc aaatccctgt agccgcccca
     4141 cgacagcgcg aggagcatgc gcccagggct gagcgcgggt agatcagagc acacaagctc
     4201 acagtccccg gcggtggggg gaggggcgcg ctgagcgggg gccagggagc tggcgcgggg
     4261 caaactggga aagtggtgtc gtgtgctggc tccgccctct tcccgagggt gggggagaac
     4321 ggtatataag tgcggtagtc gccttggacg ttctttttcg caacgggttt gccgtcagaa
     4381 cgcaggtgag tggcgggtgt ggcttccgcg ggccccggag ctggagccct gctctgagcg
     4441 ggccgggctg atatgcgagt gtcgtccgca gggtttagct gtgagcattc ccacttcgag
     4501 tggcgggcgg tgcgggggtg agagtgcgag gcctagcggc aaccccgtag cctcgcctcg
     4561 tgtccggctt gaggcctagc gtggtgtccg ccgccgcgtg ccactccggc cgcactatgc
     4621 gttttttgtc cttgctgccc tcgattgcct tccagcagca tgggctaaca aagggagggt
     4681 gtggggctca ctcttaagga gcccatgaag cttacgttgg ataggaatgg aagggcagga
     4741 ggggcgactg gggcccgccc gccttcggag cacatgtccg acgccacctg gatggggcga
     4801 ggcctgtggc tttccgaagc aatcgggcgt gagtttagcc tacctgggcc atgtggccct
     4861 agcactgggc acggtctggc ctggcggtgc cgcgttccct tgcctcccaa caagggtgag
     4921 gccgtcccgc ccggcaccag ttgcttgcgc ggaaagatgg ccgctcccgg ggccctgttg
     4981 caaggagctc aaaatggagg acgcggcagc ccggtggagc gggcgggtga gtcacccaca
     5041 caaaggaaga gggccttgcc cctcgccggc cgctgcttcc tgtgaccccg tggtctatcg
     5101 gccgcatagt cacctcgggc ttctcttgag caccgctcgt cgcggcgggg ggaggggatc
     5161 taatggcgtt ggagtttgtt cacatttggt gggtggagac tagtcaggcc agcctggcgc
     5221 tggaagtcat tcttggaatt tgcccctttg agtttggagc gaggctaatt ctcaagcctc
     5281 ttagcggttc aaaggtattt tctaaacccg tttccaggtg ttgtgaaagc caccgctaat
     5341 tcaaagcaat atggcctgga tgatgcttct cctcggactc cttgcttatg gatccggagt
     5401 cgactctgaa ttcgatatcc tcgagctagg tcagcccaag gcggcgccct cggtcactct
     5461 gttcccgccc tcctctgagg agcttcaagc caacaaggcc acactggtgt gtctcataag
     5521 tgacttctac ccgggagccg tgacagtggc ctggaaggca gatagcagcc ccgtcaaggc
     5581 gggagtggag accaccacac cctccaaaca aagcaacaac aagtacgcgg ccagcagcta
     5641 tctgagcctg acgcctgagc agtggaagtc ccacagaagc tacagctgcc aggtcacgca
     5701 tgaagggagc accgtggaga agacagtggc ccctacagaa tgttcatagc taggagcagg
     5761 tttccccaat gacacaaaac gtgcaacttg aaactccgcc tggtctttcc aggtctagag
     5821 gggtaacact ttgtactgcg tttggctcca cgctcgatcc actggcgagt gttagtaaca
     5881 gcactgttgc ttcgtagcgg agcatgacgg ccgtgggaac tcctccttgg taacaaggac
     5941 ccacggggcc aaaagccacg cccacacggg cccgtcatgt gtgcaacccc agcacggcga
     6001 ctttactgcg aaacccactt taaagtgaca ttgaaactgg tacccacaca ctggtgacag
     6061 gctaaggatg cccttcaggt accccgaggt aacacgcgac actcgggatc tgagaagggg
     6121 actggggctt ctataaaagc gctcggttta aaaagcttct atgcctgaat aggtgaccgg
     6181 aggtcggcac ctttcctttg caattactga ccctatgaat acactgactg tttgacaatt
     6241 aatcatcggc atagtatatc ggcatagtat aatacgactc actataggag ggccaccatg
     6301 aagaaacctg aactgacagc aacttctgtt gagaagtttc tcattgaaaa atttgattct
     6361 gtttctgatc tcatgcagct gtctgaaggt gaagaaagca gagccttttc ttttgatgtt
     6421 ggaggaagag gttatgttct gagggtcaat tcttgtgctg atggttttta caaagacaga
     6481 tatgtttaca gacactttgc ctctgctgct ctgccaattc cagaagttct ggacattgga
     6541 gaattttctg aatctctcac ctactgcatc agcagaagag cacaaggagt cactctccag
     6601 gatctccctg aaactgagct gccagctgtt ctgcaacctg ttgctgaagc aatggatgcc
     6661 attgcagcag ctgatctgag ccaaacctct ggatttggtc cttttggtcc ccaaggcatt
     6721 ggtcagtaca ccacttggag ggatttcatt tgtgccattg ctgatcctca tgtctatcac
     6781 tggcagactg tgatggatga cacagtttct gcttctgttg ctcaggcact ggatgaactc
     6841 atgctgtggg cagaagattg tcctgaagtc agacacctgg tccatgctga ttttggaagc
     6901 aacaatgttc tgacagacaa tggcagaatc actgcagtca ttgactggtc tgaagccatg
     6961 tttggagatt ctcaatatga ggttgccaac attttttttt ggagaccttg gctggcttgc
     7021 atggaacaac aaacaagata ttttgaaaga agacacccag aactggctgg ttcccccaga
     7081 ctgagagcct acatgctcag aattggcctg gaccaactgt atcaatctct ggttgatgga
     7141 aactttgatg atgctgcttg ggcacaagga agatgtgatg ccattgtgag gtctggtgct
     7201 ggaactgttg gaagaactca aattgcaaga aggtctgctg ctgtttggac tgatggatgt
     7261 gttgaagttc tggctgactc tggaaacagg agaccctcca caagacccag agccaaggaa
     7321 tgaatattag ctagattatc cctaatacct gccaccccac tcttaatcag tggtggaaga
     7381 acggtctcag aactgtttgt ttcaattggc catttaagtt tagtagtaaa agactggtta
     7441 atgataacaa tgcatcgtaa aaccttcaga aggaaaggag aatgttttgt ggaccacttt
     7501 ggttttcttt tttgcgtgtg gcagttttaa gttattagtt tttaaaatca gtacttttta
     7561 atggaaacaa cttgaccaaa aatttgtcac agaattttga gacccattaa aaaagttaaa
     7621 tgagaaacct gtgtgttcct ttggtcaaca ccgagacatt taggtgaaag acatctaatt
     7681 ctggttttac gaatctggaa acttcttgaa aatgtaattc ttgagttaac acttctgggt
     7741 ggagaatagg gttgttttcc ccccacataa ttggaagggg aaggaatatc atttaaagct
     7801 atgggagggt tgctttgatt acaacactgg agagaaatgc agcatgttgc tgattgcctg
     7861 tcactaaaac aggccaaaaa ctgagtcctt gggttgcata gaaagctg
//
