LOCUS       sequence-433111-       10462 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes SARS-CoV-2 spike D936Y for pseudovirus production.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10462)
  AUTHORS   Roederer AL, Cao Y, Denis KS, Sheehan ML, Li CJ, Lam EC, Gregory DJ,
            Poznansky MC, Iafrate AJ, Canaday DH, Gravenstein S, Garcia-Beltran 
            WF, Balazs AB
  TITLE     Ongoing evolution of SARS-CoV-2 drives escape from mRNA 
            vaccine-induced humoral immunity.
  JOURNAL   medRxiv [Preprint]. 2024 Mar 7:2024.03.05.24303815. doi: 
            10.1101/2024.03.05.24303815.
  PUBMED    38496628
REFERENCE   2  (bases 1 to 10462)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "medRxiv 
            [Preprint]. 2024 Mar 7:2024.03.05.24303815. doi: 
            10.1101/2024.03.05.24303815."
COMMENT     Sequence Label: pTwist-SARS-CoV-2 Spike-deltaC18 D936Y (TAT)
FEATURES             Location/Qualifiers
     source          1..10462
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    3838..4426
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3891..3911)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4309..4320)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    4479..4600
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4490..4509)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4544..4563
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     terminator      4715..4761
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      4791..4927
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    4972..5043
                     /label=3' beta-globin insulator
                     /note="insulator downstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     promoter        5088..5284
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      5135..5270
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5298..6092
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5352..5371)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5962..5981
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6103..6327
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(6523..7111)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6603..6622)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             join(7857..7925,7926..8717)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8075..8094)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     terminator      8730..8866
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    9018..9089
                     /label=5' beta-globin insulator
                     /note="insulator upstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     terminator      complement(9094..9140)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     polyA_signal    9158..9206
                     /note="synthetic polyadenylation signal"
     enhancer        9287..9590
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        9592..9794
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     9745..9765
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     intron          9928..10403
                     /label=beta-globin intron
                     /note="internally truncated intron from human beta-globin"
ORIGIN
        1 atgtttgtgt tcctcgttct cctgcccctc gtttcttccc aatgcgtgaa cctcacaact
       61 agaacgcagc tcccacccgc ttacacgaac tcttttactc gtggagtata ctatcccgat
      121 aaagtcttta ggtccagtgt ccttcattct actcaagacc ttttcctgcc attcttctca
      181 aatgtcacct ggttccatgc tatccacgtt tctggcacca acggaactaa acgatttgat
      241 aatcccgtct tgccatttaa cgatggcgta tactttgcct caacggagaa gtccaacatt
      301 atccggggat ggatatttgg caccaccctg gactccaaga cacagtctct cctcattgtg
      361 aacaacgcca ccaatgtcgt catcaaagtc tgtgaatttc aattttgtaa cgatcccttt
      421 ctcggggttt attatcataa gaataacaag tcttggatgg agtcagagtt ccgcgtttac
      481 agttccgcta acaactgtac ttttgagtac gtttcccaac catttctcat ggatcttgaa
      541 gggaagcaag gcaacttcaa gaatctccgc gaatttgtgt ttaagaatat agacggctac
      601 ttcaaaattt atagcaagca taccccgata aatctggttc gggatctccc tcaaggcttc
      661 tccgcgttgg aacccctcgt ggacttgccg attggcatca acatcacccg gttccaaacc
      721 cttcttgccc ttcatcgaag ctacctcacg cctggcgaca gtagtagtgg ttggacagcg
      781 ggcgcagcag cctactatgt gggatatttg caaccacgca cctttcttct caaatataac
      841 gaaaacggaa caattaccga tgccgtagac tgtgccctcg atcccctgtc cgaaactaaa
      901 tgcacactca agtccttcac agttgagaaa ggcatatacc aaaccagtaa cttccgcgtc
      961 caacccaccg aatccattgt tcgattccct aacatcacca atttgtgtcc tttcggtgaa
     1021 gtatttaatg ccactcgatt tgcatcagtt tatgcctgga atcgcaagcg aatatcaaat
     1081 tgtgttgcag attatagtgt cctgtataat agcgcatcat tcagcacttt taagtgttat
     1141 ggggtcagcc ctacgaaatt gaatgacttg tgttttacca acgtttacgc agattctttc
     1201 gtcatacgcg gcgacgaagt ccgacagatt gcaccggggc aaaccggaaa gatagcagac
     1261 tataattata agttgccgga tgacttcacc ggctgcgtta tagcatggaa ctccaataat
     1321 ctcgattcaa aagtcggcgg caactataat tatctttacc gcctgtttcg caagtcaaat
     1381 ctgaagccct ttgaacgtga catctccacc gaaatttatc aagctggcag caccccatgt
     1441 aatggagtgg aagggtttaa ctgttatttt ccccttcaat catacggatt tcaaccaaca
     1501 aatggggtgg gctatcaacc atatcgggtg gtagtcttga gctttgagct gctccacgca
     1561 ccggctacag tttgcggacc gaagaaaagc actaacctgg tgaagaataa atgtgtgaac
     1621 ttcaacttta atgggctcac cggaacaggg gtgctgacag aatcaaacaa gaaatttctc
     1681 ccttttcaac aatttggtag agacattgcc gataccacag atgccgtccg cgatccgcaa
     1741 acactggaaa tcctcgatat aacaccctgt agcttcggcg gggtcagcgt gataactccc
     1801 ggaacgaaca catccaacca agtggctgtc ctgtatcaag atgtcaactg taccgaagtc
     1861 cctgtggcga tacacgccga ccaactgaca cccacctgga gagtctactc aacaggttcc
     1921 aatgtctttc aaactagagc gggttgtctc attggcgctg agcatgtcaa taattcatac
     1981 gaatgtgata ttcctattgg ggcaggaatc tgtgcgtctt atcaaactca aacgaactca
     2041 ccacggcgcg ctcgctcagt agccagccag tccataatcg cctataccat gagtctcggg
     2101 gctgagaact ccgtggctta ttcaaacaat tccatcgcaa tcccaactaa ctttaccatt
     2161 agcgttacca cggaaatctt gcccgtcagc atgactaaga cgagcgtaga ctgtacgatg
     2221 tatatctgtg gggattccac cgagtgtagc aacctcttgc ttcaatacgg gtctttctgt
     2281 actcaactca accgagcatt gaccggaatt gcggtcgaac aagacaagaa tacccaagag
     2341 gtgttcgcac aagtaaagca aatatacaaa acgccgccaa tcaaagattt tggcgggttt
     2401 aattttagcc aaatactgcc agacccatct aagccttcaa aacgcagctt tatcgaggat
     2461 ctgctgttca acaaagttac gttggctgac gcgggtttca ttaagcagta tggggattgt
     2521 ttgggagaca ttgcggcccg cgaccttatc tgtgctcaaa aattcaatgg gttgacagtt
     2581 ctgccccctc tgctcactga tgagatgatt gcacagtata catcagcatt gttggcagga
     2641 accattacat ccggctggac ttttggcgcg ggtgcggccc ttcaaatacc tttcgctatg
     2701 caaatggctt acagattcaa tgggattggt gtcacgcaaa atgtattgta tgaaaatcag
     2761 aagctgatcg ctaatcaatt taacagcgcg attggcaaga tccaatattc actgtcaagc
     2821 acagcgtccg ccctcggcaa actccaagat gttgtgaatc aaaacgccca ggccctgaat
     2881 accctcgtga agcaactgag ttctaacttc ggcgcaatct ccagcgtcct caacgacata
     2941 ttgtccagac ttgataaggt ggaagccgag gtgcaaattg atcggctcat cacaggcagg
     3001 ctccagtcac tccaaactta tgtgacacaa cagttgatcc gggctgctga gatacgagca
     3061 agcgccaacc tggccgccac taaaatgtca gagtgcgtgc tcgggcagtc caaacgggtt
     3121 gacttttgtg gcaagggcta tcacttgatg tccttccccc aatccgcccc acatggagtg
     3181 gtctttctcc acgtcactta tgtccctgct caagagaaga actttacgac agctccagca
     3241 atctgtcacg atggcaaagc gcactttccc agggaaggcg ttttcgtcag taatggcacg
     3301 cattggtttg tgacccaaag gaatttctat gaaccccaaa tcataaccac tgacaataca
     3361 tttgtctcag gcaactgtga tgtggtcatt gggatcgtga acaacacggt atatgatccc
     3421 ctgcaacccg agttggatag cttcaaagag gaattggata aatactttaa gaatcatact
     3481 tcacccgatg ttgatctcgg ggacatatca ggaataaatg caagtgtcgt caacattcag
     3541 aaagagattg atcgccttaa tgaggtcgcc aagaacttga atgaatcttt gatcgacttg
     3601 caagaactgg gcaaatacga acaatatatt aaatggccct ggtacatttg gttgggcttt
     3661 atcgccggac ttatagctat cgttatggtc acaattatgt tgtgctgtat gactagctgt
     3721 tgttcctgct tgaaaggatg ttgcagttgc ggatcttgtt gtaaataaag gatccctaga
     3781 agttgtctcc tcctgcactg actgactgat acaatcgatt tctggatccg caggcctaat
     3841 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct
     3901 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg
     3961 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg
     4021 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt
     4081 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt
     4141 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg
     4201 ggcactgaca attccgtggt gttgtcgggg aagctgacgt cctttccatg gctgctcgcc
     4261 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat
     4321 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc
     4381 cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcctgt ctagcttgac
     4441 tgactgagat acagcgtacc ttcagctcac agacatgata agatacattg atgagtttgg
     4501 acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat
     4561 tgctttattt gtaaccatta taagctgcaa taaacaagtt aacaacaaca attgcattca
     4621 ttttatgttt caggttcagg gggaggtgtg ggaggttttt taaagcaagt aaaacctcta
     4681 caaatgtggt attggcccat ctctatcggt atcgtagcat aaccccttgg ggcctctaaa
     4741 cgggtcttga ggggtttttt gtgcccctcg ggccggattg ctatctaccg gcattggcgc
     4801 agaaaaaaat gcctgatgcg acgctgcgcg tcttatactc ccacatatgc cagattcagc
     4861 aacggatacg gcttccccaa cttgcccact tccatacgtg tcctccttac cagaaattta
     4921 tccttaaggt cgtcagctat cctgcaggcg atctctcgat ttcgatcaag acattccttt
     4981 aatggtcttt tctggacacc actaggggtc agaagtagtt catcaaactt tcttccctcc
     5041 ctaatctcat tggttacctt gggctatcga aacttaatta agcgatctgc atctcaatta
     5101 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     5161 cgcccattct ccgccccatc gctgactaat tttttttatt tatgcagagg ccgaggccgc
     5221 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
     5281 caaaggaggt agccaacatg attgaacaag atggattgca cgcaggttct cccgccgctt
     5341 gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg
     5401 ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg
     5461 gtgccctgaa tgaactccag gacgaggcag cgcggctatc gtggctggcc acgacgggcg
     5521 ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg
     5581 gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca
     5641 tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc
     5701 accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc
     5761 aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca
     5821 aggcgcggat gcccgacggc gaggatctcg tcgtgaccca cggcgatgcc tgcttgccga
     5881 atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg
     5941 cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg
     6001 aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg
     6061 ccttctatcg ccttcttgac gagttcttct agtatgtaag ccctgtgcct tctagttgcc
     6121 agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca
     6181 ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta
     6241 ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc
     6301 atgctgggga tgcggtgggc tctatggtta attaaccagt caagtcagct acttggcgag
     6361 atcgacttgt ctgggtttcg actacgctca gaattgcgtc agtcaagttc gatctggtcc
     6421 ttgctattgc acccgttctc cgattacgag tttcatttaa atcatgtgag caaaaggcca
     6481 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     6541 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     6601 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     6661 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     6721 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     6781 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     6841 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     6901 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     6961 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     7021 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     7081 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     7141 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     7201 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     7261 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     7321 ctgtctattt cgttcatcca tagttgcatt taaatttccg aactctccaa ggccctcgtc
     7381 ggaaaatctt caaacctttc gtccgatcca tcttgcaggc tacctctcga acgaactatc
     7441 gcaagtctct tggccggcct tgcgccttgg ctattgcttg gcagcgccta tcgccaggta
     7501 ttactccaat cccgaatatc cgagatcggg atcacccgag agaagttcaa cctacatcct
     7561 caatcccgat ctatccgaga tccgaggaat atcgaaatcg gggcgcgcct ggtgtaccga
     7621 gaacgatcct ctcagtgcga gtctcgacga tccatatcgt tgcttggcag tcagccagtc
     7681 ggaatccagc ttgggaccca ggaagtccaa tcgtcagata ttgtactcaa gcctggtcac
     7741 ggcagcgtac cgatctgttt aaacctagat attgatagtc tgatcggtca acgtataatc
     7801 gagtcctagc ttttgcaaac atctatcaag agacaggatc agcaggaggc tttcgcatga
     7861 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
     7921 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcgcgag
     7981 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
     8041 aacgctttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
     8101 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
     8161 agtattcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
     8221 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgattggag
     8281 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
     8341 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
     8401 tagcaatggc aacaaccttg cgtaaactat taactggcga actacttact ctagcttccc
     8461 ggcaacagtt gatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     8521 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
     8581 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     8641 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     8701 tgattaagca ttggtaaccg attctaggtg cattggcgca gaaaaaaatg cctgatgcga
     8761 cgctgcgcgt cttatactcc cacatatgcc agattcagca acggatacgg cttccccaac
     8821 ttgcccactt ccatacgtgt cctccttacc agaaatttat ccttaagatc ccgaatcgtt
     8881 taaactcgac tctggctcta tcgaatctcc gtcgtttcga gcttacgcga acagccgtgg
     8941 cgctcatttg ctcgtcgggc atcgaatctc gtcagctatc gtcagcttac ctttttggca
     9001 gcgatcgcgg ctcccgacat cttggaccat tagctccaca ggtatcttct tccctctagt
     9061 ggtcataaca gcagcttcag ctacctctca attcaaaaaa cccctcaaga cccgtttaga
     9121 ggccccaagg ggttatgcta tcaatcgttg cgttacacac acaaaaaacc aacacacatc
     9181 catcttcgat ggatagcgat tttattatct aactgctgat cgagtgtagc cagatctagt
     9241 aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta
     9301 cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga
     9361 cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt
     9421 tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta
     9481 ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg
     9541 actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg ctgatgcggt
     9601 tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc
     9661 accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat
     9721 gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct
     9781 atataagcag agctggttta gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt
     9841 ttgacctcca tagaagacac cgggaccgat ccagcctccc ctcgaagctt acatgtggta
     9901 ccgagctcgg atcctgagaa cttcagggtg agtctatggg acccttgatg ttttctttcc
     9961 ccttcttttc tatggttaag ttcatgtcat aggaagggga gaagtaacag ggtacacata
    10021 ttgaccaaat cagggtaatt ttgcatttgt aattttaaaa aatgctttct tcttttaata
    10081 tacttttttg tttatcttat ttctaatact ttccctaatc tctttctttc agggcaataa
    10141 tgatacaatg tatcatgcct ctttgcacca ttctaaagaa taacagtgat aatttctggg
    10201 ttaaggcaat agcaatattt ctgcatataa atatttctgc atataaattg taactgatgt
    10261 aagaggtttc atattgctaa tagcagctac aatccagcta ccattctgct tttattttat
    10321 ggttgggata aggctggatt attctgagtc caagctaggc ccttttgcta atcatgttca
    10381 tacctcttat cttcctccca cagctcctgg gcaacgtgct ggtctgtgtg ctggcccatc
    10441 actttggcaa aggcgggccg cc
//
