LOCUS       sequence-43312-e         793 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 793)
  AUTHORS   Ferrer-Vaquer A, Piliszek A, Tian G, Aho RJ, Dufort D, Hadjantonakis
            AK
  TITLE     A sensitive and bright single-cell resolution live imaging reporter 
            of Wnt/ss-catenin signaling in the mouse.
  JOURNAL   BMC Dev Biol. 2010 Dec 21;10:121.
  PUBMED    21176145
REFERENCE   2  (bases 1 to 793)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Dev 
            Biol."; date: "2010-12-21"; volume: "10"; pages: "121"
COMMENT     Sequence Label: Tcf/Lef:H2B-GFP
FEATURES             Location/Qualifiers
     source          1..793
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     11..30
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     270..291
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      333..342
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(355..732)
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAVTKYTSAK"
ORIGIN
        1 ctcggcgcgg gtcttgtagt tgccgtcgtc cttgaagaag atggtgcgct cctggacgta
       61 gccttcgggc atggcggact tgaagaagtc gtgctgcttc atgtggtcgg ggtagcggct
      121 gaagcactgc acgccgtagg tcagggtggt cacgagggtg ggccagggca cgggcagctt
      181 gccggtggtg cagatgaact tcagggtcag cttgccgtag gtggcatcgc cctcgccctc
      241 gccggacacg ctgaacttgt ggccgtttac gtcgccgtcc agctcgacca ggatgggcac
      301 caccccggtg aacagctcct cgcccttgct caccatggtg gcgaccggtg gatccttagc
      361 gctggtgtac ttggtgacgg ccttagtacc ctcggacacg gcgtgcttgg ccaactcccc
      421 aggcagcagc aggcgcacgg ccgtctggat ctccctggag gtgatggtcg agcgcttgtt
      481 gtaatgcgcc aggcgggaag cctcacctgc gatgcgctcg aaaatgtcgt tcacaaacga
      541 attcatgatg cccatggcct tggacgaaat gccggtgtca gggtggacct gcttcagaac
      601 cttgtacaca tagatggaat agctctcctt gcggctgcgc ttgcgcttct tgccgccttt
      661 cttctgcgcc ttagtcaccg ccttcttgga gccctttttc ggggcgggag cagacttcgc
      721 tggctctggc atggtggcga attcgaagct tgagctcgag atctgagtcc ggtagcgcta
      781 gctgtgcttc tgg
//
