LOCUS       sequence-433175-        8811 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8811)
  AUTHORS   Beilina A, Van Der Brug M, Ahmad R, Kesavapany S, Miller DW, Petsko 
            GA, Cookson MR
  TITLE     Mutations in PTEN-induced putative kinase 1 associated with 
            recessive parkinsonism have differential effects on protein 
            stability.
  JOURNAL   Proc Natl Acad Sci U S A. 2005 Apr 19. 102(16):5703-8.
  PUBMED    15824318
REFERENCE   2  (bases 1 to 8811)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2005 Apr 19. 102(16):5703-8."
COMMENT     Sequence Label: pLenti6-DEST PINK1-V5 KD
FEATURES             Location/Qualifiers
     source          1..8811
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..229
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             230..410
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    457..582
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1075..1308
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1493..1537
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     enhancer        1816..2119
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2120..2323
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2274..2294
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2320..2344
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    2440..2464
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2482..4224
                     /codon_start=1
                     /product="human PTEN-induced putative kinase 1, a 
                     mitochondria serine/threonine protein kinase"
                     /label=PINK1
                     /translation="MAVRQALGRGLQLGRALLLRFTGKPGRAYGLGRPGPAAGCVRGER
                     PGWAAGPGAEPRRVGLGLPNRLRFFRQSVAGLAARLQRQFVVRAWGCAGPCGRAVFLAF
                     GLGLGLIEEKQAESRRAVSACQEIQAIFTQKSKPGPDPLDTRRLQGFRLEEYLIGQSIG
                     KGCSAAVYEATMPTLPQNLEVTKSTGLLPGRGPGTSAPGEGQERAPGAPAFPLAIAMMW
                     NISAGSSSEAILNTMSQELVPASRVALAGEYGAVTYRKSKRGPKQLAPHPNIIRVLRAF
                     TSSVPLLPGALVDYPDVLPSRLHPEGLGHGRTLFLVMKNYPCTLRQYLCVNTPSPRLAA
                     MMLLQLLEGVDHLVQQGIAHRALKSDNILVELDPDGCPWLVIAAFGCCLADESIGLQLP
                     FSSWYVDRGGNGCLMAPEVSTARPGPRAVIDYSKADAWAVGAIAYEIFGLVNPFYGQGK
                     AHLESRSYQEAQLPALPESVPPDVRQLVRALLQREASKRPSARVAANVLHLSLWGEHIL
                     ALKNLKLDKMVGWLLQQSAATLLANRLTEKCCVETKMKMLFLANLECETLCQAALLLCS
                     WRAAL"
     protein_bind    complement(4244..4268)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4321..4362
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(4399..4419)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4404..4733
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4584..4719
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        4781..4828
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             4847..5245
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             5332..5565
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5637..5758
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5674..5693)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5728..5747
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5798..5933
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5953..5972)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5954..5972)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5982..5999)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5982..5998)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5991..6013)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6140..6595
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6227..6246)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6437..6458
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6621..6725
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6726..6794,6795..7586)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6944..6963)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7757..8345
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8246..8265
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8499..8516
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8633..8654
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8669..8674,8675..8692,8693..8699)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8707..8723
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8712..8734
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8731..8747
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8731..8747
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8766..8786
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8768..8786
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcgata
     1801 agcttgggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa
     1861 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac
     1921 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca
     1981 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg
     2041 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt
     2101 agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg
     2161 gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg
     2221 gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat
     2281 gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctcgtttag tgaaccgtca
     2341 gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagacacc gactctagag
     2401 gatccactag tccagtgtgg tggaattctg cagatatcaa caagtttgta caaaaaagca
     2461 ggcttgaagg aattcggtac catggcggtg cgacaggcgc tgggccgcgg cctgcagctg
     2521 ggtcgagcgc tgctgctgcg cttcacgggc aagcccggcc gggcctacgg cttggggcgg
     2581 ccgggcccgg cggcgggctg tgtccgcggg gagcgtccag gctgggccgc aggaccgggc
     2641 gcggagcctc gcagggtcgg gctcgggctc cctaaccgtc tccgcttctt ccgccagtcg
     2701 gtggccgggc tggcggcgcg gttgcagcgg cagttcgtgg tgcgggcctg gggctgcgcg
     2761 ggcccttgcg gccgggcagt ctttctggcc ttcgggctag ggctgggcct catcgaggaa
     2821 aaacaggcgg agagccggcg ggcggtctcg gcctgtcagg agatccaggc aatttttacc
     2881 cagaaaagca agccggggcc tgacccgttg gacacgagac gcttgcaggg ctttcggctg
     2941 gaggagtatc tgatagggca gtccattggt aagggctgca gtgctgctgt gtatgaagcc
     3001 accatgccta cattgcccca gaacctggag gtgacaaaga gcaccgggtt gcttccaggg
     3061 agaggcccag gtaccagtgc accaggagaa gggcaggagc gagctccggg ggcccctgcc
     3121 ttccccttgg ccatcgcgat gatgtggaac atctcggcag gttcctccag cgaagccatc
     3181 ttgaacacaa tgagccagga gctggtccca gcgagccgag tggccttggc tggggagtat
     3241 ggagcagtca cttacagaaa atccaagaga ggtcccaagc aactagcccc tcaccccaac
     3301 atcatccggg ttctccgcgc cttcacctct tccgtgccgc tgctgccagg ggccctggtc
     3361 gactaccctg atgtgctgcc ctcacgcctc caccctgaag gcctgggcca tggccggacg
     3421 ctgttcctcg ttatgaagaa ctatccctgt accctgcgcc agtacctttg tgtgaacaca
     3481 cccagccccc gcctcgccgc catgatgctg ctgcagctgc tggaaggcgt ggaccatctg
     3541 gttcaacagg gcatcgcgca cagagccctg aaatccgaca acatccttgt ggagctggac
     3601 ccagacggct gcccctggct ggtgatcgca gcttttggct gctgcctggc tgatgagagc
     3661 atcggcctgc agttgccctt cagcagctgg tacgtggatc ggggcggaaa cggctgtctg
     3721 atggccccag aggtgtccac ggcccgtcct ggccccaggg cagtgattga ctacagcaag
     3781 gctgatgcct gggcagtggg agccatcgcc tatgaaatct tcgggcttgt caatcccttc
     3841 tacggccagg gcaaggccca ccttgaaagc cgcagctacc aagaggctca gctacctgca
     3901 ctgcccgagt cagtgcctcc agacgtgaga cagttggtga gggcactgct ccagcgagag
     3961 gccagcaaga gaccatctgc ccgagtagcc gcaaatgtgc ttcatctaag cctctggggt
     4021 gaacatattc tagccctgaa gaatctgaag ttagacaaga tggttggctg gctcctccaa
     4081 caatcggccg ccactttgtt ggccaacagg ctcacagaga agtgttgtgt ggaaacaaaa
     4141 atgaagatgc tctttctggc taacctggag tgtgaaacgc tctgccaggc agccctcctc
     4201 ctctgctcat ggagggcagc cctgaagctc gagtgcggcc gcaacccagc tttcttgtac
     4261 aaagtggttg atatccagca cagtggcggc cgctcgagtc tagagggccc gcggttcgaa
     4321 ggtaagccta tccctaaccc tctcctcggt ctcgattcta cgcgtaccgg ttagtaatga
     4381 gtttggaatt aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc
     4441 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt
     4501 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
     4561 tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc
     4621 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tctgcctctg
     4681 agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc
     4741 cgggagcttg tatatccatt ttcggatctg atcagcacgt gttgacaatt aatcatcggc
     4801 atagtatatc ggcatagtat aatacgacaa ggtgaggaac taaaccatgg ccaagccttt
     4861 gtctcaagaa gaatccaccc tcattgaaag agcaacggct acaatcaaca gcatccccat
     4921 ctctgaagac tacagcgtcg ccagcgcagc tctctctagc gacggccgca tcttcactgg
     4981 tgtcaatgta tatcatttta ctgggggacc ttgtgcagaa ctcgtggtgc tgggcactgc
     5041 tgctgctgcg gcagctggca acctgacttg tatcgtcgcg atcggaaatg agaacagggg
     5101 catcttgagc ccctgcggac ggtgccgaca ggtgcttctc gatctgcatc ctgggatcaa
     5161 agccatagtg aaggacagtg atggacagcc gacggcagtt gggattcgtg aattgctgcc
     5221 ctctggttat gtgtgggagg gctaagcaca attcgagctc ggtaccttta agaccaatga
     5281 cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc
     5341 taattcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct ctctggttag
     5401 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
     5461 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
     5521 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc
     5581 atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggaact
     5641 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     5701 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
     5761 atgtctggct ctagctatcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
     5821 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
     5881 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctagggac
     5941 gtacccaatt cgccctatag tgagtcgtat tacgcgcgct cactggccgt cgttttacaa
     6001 cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct
     6061 ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc
     6121 agcctgaatg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     6181 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
     6241 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
     6301 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
     6361 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
     6421 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
     6481 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
     6541 ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttaggtg
     6601 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     6661 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     6721 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
     6781 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     6841 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     6901 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     6961 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     7021 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     7081 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     7141 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     7201 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     7261 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
     7321 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
     7381 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     7441 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     7501 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     7561 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     7621 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     7681 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     7741 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     7801 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     7861 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     7921 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     7981 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     8041 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     8101 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     8161 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     8221 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     8281 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     8341 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     8401 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
     8461 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
     8521 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
     8581 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga
     8641 gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt
     8701 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca
     8761 agcgcgcaat taaccctcac taaagggaac aaaagctgga gctgcaagct t
//
