LOCUS       sequence-433261-       10266 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Baculovirus transfer vector to secrete Fzd4 cysteine-rich domain and
            linker (residues 42-203, codon-optimized for insect cell expression,
            with a C-terminal His tag) in insect cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10266)
  AUTHORS   Bruguera ES, Mahoney JP, Weis WI
  TITLE     The co-receptor Tspan12 directly captures Norrin to promote 
            ligand-specific beta-catenin signaling
  JOURNAL   bioRxiv 2024.02.03.578714
REFERENCE   2  (bases 1 to 10266)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.02.03.578714"
COMMENT     Sequence Label: mFzd4CRDL(42-203)-6xHis_pAcGP67a
FEATURES             Location/Qualifiers
     source          1..10266
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             681..698
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     misc_recomb     1132..1837
                     /label=baculovirus recombination region (ORF1629)
                     /note="contains part of ORF1629"
     CDS             1132..1720
                     /codon_start=2
                     /product="baculovirus capsid-associated protein"
                     /label=ORF1629
                     /note="required for viral replication"
                     /translation="IPKSSTTNLIADVLADTINRRRVAMAKSSSEATSNDEGWDDDDNR
                     PNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVEPLLQNKTQTNIDKAR
                     LLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDLIFKFRYKDAENHLIF
                     ALTYHPKDYKFNELLKYVQQLSVNQQRTESSA"
     3'UTR           1377..1749
                     /label=polyhedrin 3' UTR
                     /note="3' UTR from the Autographa californica 
                     nucleopolyhedrovirus polyhedrin gene (Suzuki et al., 2006)"
     CDS             complement(2347..2364)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             2699..2710
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     sig_peptide     complement(2863..2922)
                     /codon_start=1
                     /gene="Autographa californica gp64"
                     /product="signal sequence from the baculovirus envelope 
                     glycoprotein GP64"
                     /label=GP64 signal sequence
                     /translation="MVSAIVLYVLLAAAAHSAFA"
     promoter        complement(3019..3110)
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     complement(3077..3094)
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     misc_recomb     3114..3941
                     /label=baculovirus recombination region (lef2/ORF603)
                     /note="contains ORF603 and part of lef2"
     CDS             3174..3779
                     /codon_start=1
                     /product="baculovirus ORF603 protein"
                     /label=ORF603
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNGV
                     IELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIHPI
                     KKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKIILSQ
                     INCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR"
     CDS             complement(3817..3941)
                     /codon_start=3
                     /product="baculovirus late expression factor 2"
                     /label=lef2
                     /translation="HLLIKSQDVYKPPNCQKMKTVDKLCPFAGNCKGLNPICNY"
     primer_bind     complement(7114..7131)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7114..7130)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7123..7145)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(7339..7358)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7458..7480
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7518..7536)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7604..7708
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7709..7777,7778..8569)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7927..7946)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8740..9328
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9229..9248
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9482..9499
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9616..9637
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9652..9657,9658..9675,9676..9682)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9690..9706
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9695..9717
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9714..9730
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9714..9730
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 caaagcgccc gttgattatt ttataccgag ttttgacgtt tttgcagttt tcgtaaaatt
       61 gtaccagcgt ttgcagcgta gcatttgtgg tggccatgac gaatcgtaga tatgaatctg
      121 tacaatctta tttattcaat aataggaaca acaaaattga tgcgcatcaa ttttttgaac
      181 gtgtcgatac tgcggaagcg caaattatca aaaacaacat ttacgacaac acagtggtgc
      241 tgaacagaga tgttctttta aatattttaa agcttgccaa cgacgttttt gacaacaaag
      301 catacatgta cgtcgacgac agcgaagtgt ctcgccacta caatgctgta gtaaaaatga
      361 aaaggcttgt tatcggcgtg cgagcttgag acaatctctt tacaatacaa ttgcttacat
      421 tgagcggttg ttaaatatcg gcacggtaaa cgatagcgaa attacaatgc ttatagcaga
      481 tttttacgat ttgtattcca attataatat tgaattgcca ccaccccaag cgctgcctcg
      541 aagcagacga ccttccgttg tgcagccagc ggcgcctgcg ccggtgccca caatcgtgcg
      601 cgaacaaact aaaccagaac aaattatacc ggcggcaccg ccgccaccac cttctcccgt
      661 gcctaacatt ccagcgcctc caccaccacc accaccatcg atgtctgaat tgccgcccgc
      721 tccaccaatg ccgacggaac ctcaacccgc tgcaccttta gacgacagac aacaattgtt
      781 ggaagctatt agaaacgaaa aaaatcgcac tcgtctcaga ccggtcaaac caaaaacggc
      841 gcccgaaacc agtacaatag ttgaggtgcc gactgtgttg cctaaagaga catttgagcc
      901 taaaccgccg tctgcatcac cgccaccacc tccgcctccg cctccgccgc cagccccgcc
      961 tgcgcctcca ccgatggtag atttatcatc agctccacca ccgccgccat tagtagattt
     1021 gccgtctgaa atgttaccac cgcctgcacc atcgctttct aacgtgttgt ctgaattaaa
     1081 atcgggcaca gttagattga aacccgccca aaaacgcccg caatcagaaa taattccaaa
     1141 aagctcaact acaaatttga tcgcggacgt gttagccgac acaattaata ggcgtcgtgt
     1201 ggctatggca aaatcgtctt cggaagcaac ttctaacgac gagggttggg acgacgacga
     1261 taatcggcct aataaagcta acacgcccga tgttaaatat gtccaagctt tatttaacgt
     1321 gtttacgtcg agtcaattgt acactaacga cagtgatgaa agaaatacaa aagcgcataa
     1381 tattttgaac gacgtcgaac ctttattaca aaacaaaaca caaacgaata tcgacaaagc
     1441 tagattgctg ctacaagatt tggcaagttt tgtggcgttg agcgaaaatc cattagatag
     1501 tccagccatc ggttcggaaa aacaaccctt gtttgaaact aatcgaaacc tattttacaa
     1561 atctattgag gatttaatat ttaaattcag atataaagac gctgaaaatc atttgatttt
     1621 cgctctaaca taccacccta aagattataa atttaatgaa ttattaaaat acgtacaaca
     1681 attgtctgta aatcaacaac gcacagaatc tagcgcttaa taaatgtact aataacaatg
     1741 tatcgtgttt taatacgccg gaccagtgaa cagaggtgcg tctggtgcaa actcctttac
     1801 tttgaacacc agggaaactt caaggagaat ttcctcctct tcagcagagt cggtaccgat
     1861 gtaaacgatg ggcttgtaga agttctccca gatgacacga tcgatgaact gttcgaacga
     1921 gttggtgtac tcagagtgaa ggttcattat tgggcagccg ccgcccttct tagccaggct
     1981 gatgcggtac tcgttgttgc tgcccaccca tgaaggctcg acgatcctaa tcacgtcatg
     2041 aggtacatag tcggggtcgc aacgcagagc gtgttgggcc aggaatttgt aacaacggtt
     2101 gggtctagtg ggacgcatgt tgacaacaag gaaaacatcc atcacttctt ggtcgttaac
     2161 aatggggaag ctgtcttcca tgaagcgggt ccaagtttcc ctgtagaact cttttccttt
     2221 ccatccaacg acaagcttca tcgtgtcggg tttaacatta cggatttcct tgaagagagt
     2281 gagtttttgg ttcttgccgg gtcccaggaa aggatcagat ctgcagcggc cgctccggaa
     2341 ttcctaatga tgatgatgat gatgtccctt cagcacgcaa ttcaggctgc gcttcaccca
     2401 aatgtactgg tccgagttgg agcccaccga atggcactcc tctccgggct ggattggcgt
     2461 cttgtgtggc agcggcacct cctcgtctcc tggtccctcc atgcacatat gattgtgatc
     2521 gttctgcggg gggaacttcg agcaattcag ggtatcgggc caggcaaagc cgaactcgcg
     2581 cagcactggc tcgcagcggc gcttcacgga caggcacatt cctccgcatg gtccgattgg
     2641 aatgttgatc ttctcggtgc acatcggcac atacacgctg cacagaaaga actgcagctg
     2701 ggagctgcag ccgtactgga tcaggggggt gaaggttgtc agctgcagct cggcatctgt
     2761 ctgcagctcg tggcccacca ggtttggcat cttggtcaca ttgtatccca ggttctggca
     2821 catggcaatg cggattgggt cgcagcggcg ctcgggatcc gccgcaaagg cagaatgcgc
     2881 cgccgccgcc aaaagcacat ataaaacaat agcgcttacc atcttgcttg tgtgttcctt
     2941 attgaagcct tggtgtgact gatttactag tagcatagat ccgcgcccga tggtgggacg
     3001 gtatgaataa tccggaatat ttataggttt ttttattaca aaactgttac gaaaacagta
     3061 aaatacttat ttatttgcga gatggttatc attttaatta tctccatgat atcagcaact
     3121 atatattgat agacatttcc agtttgtgat attagtttgt gcgtctcatt acaatggctg
     3181 ttatttttaa caacaaacaa ctgctcgcag acaatagtat agaaaaggga ggtgaactgt
     3241 ttttgtttaa cggttcgtac aacattttgg aaagttatgt taatccggtg ctgctaaaaa
     3301 atggtgtaat tgaactagaa gaagctgcgt actatgccgg caacatattg tacaaaaccg
     3361 acgatcccaa attcattgat tatataaatt taataattaa agcaacacac tccgaagaac
     3421 taccagaaaa tagcactgtt gtaaattaca gaaaaactat gcgcagcggt actatacacc
     3481 ccattaaaaa agacatatat atttatgaca acaaaaaatt tactctatac gatagataca
     3541 tatatggata cgataataac tatgttaatt tttatgagga gaaaaatgaa aaagagaagg
     3601 aatacgaaga agaagacgac aaggcgtcta gtttatgtga aaataaaatt atattgtcgc
     3661 aaattaactg tgaatcattt gaaaatgatt ttaaatatta cctcagcgat tataactacg
     3721 cgttttcaat tatagataat actacaaatg ttcttgttgc gtttggtttg tatcgttaat
     3781 aaaaaacaaa tttgacattt ataattgttt tattattcaa taattacaaa taggattgag
     3841 acccttgcag ttgccagcaa acggacagag cttgtcgaca gttttcattt tttggcagtt
     3901 tggtggttta tacacatctt gggatttgat taaaagatgc atgacttcac ccacacactt
     3961 ggagtcgtgg ttgtagaaat gcgtcagagc cttgattaaa catcggtttt cacacttggc
     4021 acacgaaacc acgtttgcaa tgtaacagtt tagtataaac ctcttcctgt acataccgcc
     4081 gcgcggtaca ttgttttctt tcaaatcgtt caatattttt tttatacacg gaggcatatt
     4141 aatcttgttg ttcagcatgt cgacgacatt tttctttcct tctacgcatt ctttcataca
     4201 aatgtttctc ttgctgcgtt ttttaaaatt ggaattttta atttcattaa tagtgggcgg
     4261 agccgttaac agcatgtaca atcgcagtcc ggaaattttg accaaaaccc cgccattgta
     4321 gaataccgtg tatggagtta gggtcaattt atcaataaaa tcatcgggat caattaaata
     4381 tgtagctttc ttgtctaaac atgacgctct aatgagcgga ctccacacgt tatacgatgc
     4441 attcgccata cttcgcggct tctcgcacca gcggtcctga ttcgactaac ggtcgattgg
     4501 atggcttaat agttttaacg tcaccgacat actcggtaat tttgtagtcg gcagttcttt
     4561 tgggcgtgat cgttgtcact acgggggtca tgtcatcgct tcgtaaaaac ttattacaat
     4621 caaagctcgt gccggaacgc gtgcacagat cgtgcacgtt gcgtcgcaag ctatttaatt
     4681 cgcgctttat ttgatctcta tcatagtcgt gttgagatgc gacaacgggc gcattgttaa
     4741 ttcttgacgt gcgatacata atatattatt ggataaagtt gcattaaatg aaactaactt
     4801 acaagatggc tagtttgtta aaatacgcgc tgcgcttgac tcgggaatac aaagaaaaca
     4861 ttattccaca ctttgatcac ttgactcgat tgcgcgattt aatcgacggc atgattaaaa
     4921 gcgaggatgt acaacgtttt aatcgcacta atcgcaatga tttaatttcg gcttgcatgc
     4981 aaatcaacgt tcggacgtac atgcccaacg ccacgataga tatgcgcaaa caacccaact
     5041 gtatatattt tcgaatttgc caatattgcc acttggaggc cgacgtgcct tcgcccgacg
     5101 atcattcggt gtacagatac ttgtgcgtcg cgtgcggcac gccgctggtc atcgaccacc
     5161 cgctcgacgt gttcggccac acggaggaag gcgtcaacga actgctcgag gtgcagcgag
     5221 tcaacgcggg cggggagttg taggcgtcat aactatttat taaataagat aatttaaaaa
     5281 atcgccgtta atatgcaaat caaaactgta ctattggctt ttgcgatgtt tgccgcgttg
     5341 aacgcgcaac acgttttagc cgcttgcgcc gagaccggcg ccgtctgcgt tcataacgac
     5401 gagtgttgta gcggcgcatg ttcccccata tttaattatt gcttaccaca ataagtttag
     5461 tttgcgttaa aaaacaatag ttgttgcaaa atcgacacac gtgtatcaaa atggctcgcg
     5521 ttaaaattgg agaatttaaa tttggagaag atacgttcaa cctgcggtac gtgctagagc
     5581 gcgaccaaca ggtccggttc gtggccaagg acgtcgctaa cagtttaaaa tatacagttt
     5641 gtgataaagc tatacgtgtt catgtagaca ataaatacaa atcgttattt gagcagacca
     5701 tccaaaatgg gggccctacc tctaacagcg tcgtgaaaag gggcgacccg ctgtatttgc
     5761 agccgcatac agtgctcatt actaaatccg gcgtgattca gctgattatg aagtccaaat
     5821 tgccttacgc catagaatta caagaatggc ttttagaaga agtaattcct caagtgctct
     5881 gcaccggcaa gtacgatccc gcgatcaaac aacgggagga ggagagcaaa cagttggtga
     5941 ctaagctgat tgcgacattc accgagcaca caaacgcgct gcaagcggtg gtggcgcaaa
     6001 aaaccgagga acttgttaaa aaacaagagt ttattgaacg catcgttgcc atcaaggaca
     6061 agcagatcga ggccaaagat ttgcaggtca cgcgcgtcat gactgatcta aaccgcatgt
     6121 acaccggctt ccaggaaacc atgcaaaaga aagacgaaat aatgcagaaa aaagacgcgc
     6181 aggtcaccga tttagttgcc aaagtggtgg atttgtcgga tcgcgcggtt caataccccg
     6241 cggacaagcg caaacatccg gtgttgtgcg tgacgcgcga cggcactacg tttacggcta
     6301 ttaccggcca aaagacgtac gtggaaaacc aaaagcataa acgtaacatc aacgttgcca
     6361 acattgtggt ggagaatatc cggcctaatc ctaccgtcga ttggaacaac gccactgatc
     6421 ggctacaagc taaacggagc aagcgaagca tagttttggt tcgctggaag aagcgcaaca
     6481 atttgaaaat aggataaagt atttgttaaa gaatgcaaat aatattaatt aaattaataa
     6541 atcttgaacg taattttgtc tttcaatttt gtgacctctg gctttttcga atctatctat
     6601 ggcttcctgc ggcgcaatac ccagggtgtg cattaaatat ctgcacacca tgtaaccggt
     6661 gcgattaata ccgtgtgtgc agtgcacgcc caccaacatg ccgggacact tttctgtaaa
     6721 ttcttttacc gtgtcaataa attcttgaac tatgctttca ggcggcaaag tctggccagg
     6781 tacttgaatt tttttgtata acaggcccgc ccgcaaaaaa tgcacaccat cataatattt
     6841 agacgtgttg gttaaatcga ttattgctcc aatactaggg ttttgtttta ctatctgttc
     6901 tgcggtccac atatcttctt cgctagtcac gtacgcaaac aactcgggcc gcaaaggtgt
     6961 tttaaaacat atcagattag aatcttttat aacttggccg cattgtaaat agttgtgcca
     7021 acgcgcggga aacattttac acgtgctttc aatcttatct cataaacgca actaaatatg
     7081 atccttcaac tcgctttacg agccaagctt ggcactggcc gtcgttttac aacgtcgtga
     7141 ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag
     7201 ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa
     7261 tggcgaatgg cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg
     7321 catatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca
     7381 cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag
     7441 acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa
     7501 acgcgcgaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat
     7561 aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     7621 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     7681 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
     7741 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
     7801 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
     7861 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
     7921 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
     7981 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
     8041 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     8101 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     8161 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     8221 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
     8281 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     8341 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     8401 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
     8461 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     8521 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     8581 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     8641 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     8701 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     8761 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     8821 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     8881 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     8941 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     9001 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     9061 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     9121 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     9181 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     9241 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     9301 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     9361 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     9421 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     9481 cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc
     9541 gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag
     9601 tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt
     9661 tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa
     9721 cagctatgac catgattacg aattggccgc cggttagcaa cttgtatgtt aacacgccga
     9781 cggcgtacca gtcaaacgaa cgtgcatagt tgtggtgtcg aattttttcc ggactaaaat
     9841 actccaacgt gccgtcgtgc acgctaagtg agttttcgtg tttgcacaat ccgtaatcgc
     9901 aaacatacac gcgatcaagt gcttcgaaat ataagacatt ttcgagtttt atgtcgttgt
     9961 gtatgaaatt gtgcttgtgc aaatcgttga gcgcttcaca cagctgtcta ataatattgc
    10021 taacaagttg gtacgaaagc tcgcctttaa tttgtagtgt ttcaaataaa tcgggacagt
    10081 caatgtagtc catcacgatc acttggttgt tgatggaacc gtgattaaaa tagattttta
    10141 taaaatttgg gtggtcgctc attaactggt gaacttttat ttcgtcagcg ttaaaattgt
    10201 gcgccgaaat tgttttctgc aaatacaatt tgctagtggg cttgtgcgat aaaatagata
    10261 ttttgc
//
