LOCUS       sequence-433659-       11392 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Dox inducible WT1 cDNA with exon 9 mutation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11392)
  AUTHORS   Potluri S, Assi SA, Chin PS, Coleman DJL, Pickin A, Moriya S, Seki 
            N, Heidenreich O, Cockerill PN, Bonifer C
  TITLE     Isoform-specific and signaling-dependent propagation of acute 
            myeloid leukemia by Wilms tumor 1.
  JOURNAL   Cell Rep. 2021 Apr 20;35(3):109010. doi: 
            10.1016/j.celrep.2021.109010.
  PUBMED    33882316
REFERENCE   2  (bases 1 to 11392)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2021-04-20"; volume: "35(3):109010. doi"; pages: " 
            10.1016/j.celrep.2021.109010"
COMMENT     Sequence Label: pCW57.1 Ex9 mut WT1
FEATURES             Location/Qualifiers
     source          1..11392
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(292..303)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     protein_bind    complement(711..735)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(756..1070)
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     primer_bind     complement(759..783)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    830..848
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    866..884
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    901..919
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    937..955
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    973..991
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1008..1026
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1044..1062
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     misc_feature    1124..1241
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(1454..1498)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1683..1916
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    2409..2534
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             2581..2761
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(2762..2988)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(3016..3036)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(3016..3034)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3055..3071)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3055..3071)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3068..3090)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3079..3095
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3103..3109,3110..3127,3128..3133))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3148..3169
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3286..3303)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3457..4045)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3537..3556)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4216..5007,5008..5076))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4839..4858
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5077..5148)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      5148..5283
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    5323..5444
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5334..5353)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5388..5407
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             5516..5749
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     CDS             5954..5965
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     6363..6383
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(join(6423..6431,6432..6467,6468..6470,6471..6506,
                     6507..6509,6510..6545,6546..6551,6552..7169))
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     CDS             complement(7170..7223)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(7224..7820)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7304..7324)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     7801..7820
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(7830..8340)
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     complement(7893..7911)
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             complement(8350..8367)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     protein_bind    8385..8409
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(join(8459..9172,9173..9175,9176..9178))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(8504..8525)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     8853..8872
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     9112..9133
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     misc_feature    9190..9742
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    9192..9759
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(9352..9371)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     9581..9598
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     protein_bind    9782..9806
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tatggtttct caccagtgtg cttcctgctg tgcatctgta agtgggacag cttaaaatat
       61 ctcttattgc agcctgggta agcacacatg aaggggcgtt tctcactggt ctcagatgcc
      121 gaccgtacaa gagtcggggc tactccaggc acacgtcgca catcctgaat gcctctgaag
      181 acaccgtgcg tgtgtattct gtattgggct ccgcagagga tgggcgttgt gtggttatcg
      241 ctctcgtacc ctgtgctgtg gttgctctgc ccttctgtcc atttcactga gctggagctc
      301 ccagcagcaa ctccctttaa ggtggctcct aagttcatct gattccaggt catgcattca
      361 agctgggatg tcatttggta taaattgtca ctgctgtagg gcgtcctcag cagcaaagcc
      421 tggctgccgg tgcagctgtc ggtgggggtg tggcagccat agaccggggg cggcaccgag
      481 tactgctgct cacccagcga gccctgctgg cccatgggat cctcatgctt gaatgagtgg
      541 ttggggaact gcgccgcatg gtgcgagggc gtgtgaccgt agctgggcgt cccgtcgaag
      601 gtgaccgtgc tgtaaccctg attgcgaata gcgggctggc tctcgaggca gctgggcagg
      661 tagggcgcgt taggaaacat ggtggcgtcg actgaattgg ttccggcgcc agcctgcttt
      721 tttgtacaaa cttgttgatg ctagccaatt ctccaggcga tctgacggtt cactaaacga
      781 gctctgctta tataggcctc ccaccgtaca cgcctacctc gacatacgtt ctctatcact
      841 gatagggagt aaactcgaca tacgttctct atcactgata gggataaact cgacatacgt
      901 tctctatcac tgatagggag taaactcgac atacgttctc tatcactgat agggagtaaa
      961 ctcgacatac gttctctatc actgataggg agtaaactcg acatcgttct ctatcactga
     1021 tagggagtaa actcgacata cgttctctat cactgatagg gagtaaactc gacatatcga
     1081 ttcgcggcca aagtggatct ctgctgtccc tgtaataaac ccgaaaattt tgaatttttg
     1141 taatttgttt ttgtaattct ttagtttgta tgtctgttgc tattatgtct actattcttt
     1201 cccctgcact gtacccccca atcccccctt ttcttttaaa attgtggatg aatactgcca
     1261 tttgtctcga ggtcgagaat tgtcccctcg gggttgggag gtgggtctga aacgataatg
     1321 gtgaatatcc ctgcctaact ctattcacta tagaaagtac agcaaaaact attcttaaac
     1381 ctaccaagcc tcctactatc attatgaata attttatata ccacagccaa tttgttatgt
     1441 taaaccaatt ccacaaactt gcccatttat ctaattccaa taattcttgt tcattctttt
     1501 cttgctggtt ttgcgattct tcaattaagg agtgtattaa gcttgtgtaa ttgttaattt
     1561 ctctgtccca ctccatccag gtcgtgtgat tccaaatctg ttccagagat ttattactcc
     1621 aactagcatt ccaaggcaca gcagtggtgc aaatgagttt tccagagcaa ccccaaatcc
     1681 ccaggagctg ttgatccttt aggtatcttt ccacagccag gattcttgcc tggagctgct
     1741 tgatgcccca gactgtgagt tgcaacagat gctgttgcgc ctcaatagcc ctcagcaaat
     1801 tgttctgctg ctgcactata ccagacaata attgtctggc ctgtaccgtc agcgtcattg
     1861 acgctgcgcc catagtgctt cctgctgctc ccaagaaccc aaggaacaaa gctcctattc
     1921 ccactgctct tttttctctc tgcaccactc ttctctttgc cttggtgggt gctactccta
     1981 atggttcaat ttttactact ttatatttat ataattcact tctccaattg tccctcatat
     2041 ctcctcctcc aggtctgaag atcagcggcc gcttgctgtg cggtggtctt acttttgttt
     2101 tgctcttcct ctatcttgtc taaagcttcc ttggtgtctt ttatctctat cctttgatgc
     2161 acacaataga gggttgctac tgtattatat aatgatctaa gttcttctga tcctgtctga
     2221 agggatggtt gtagctgtcc cagtatttgt ctacagcctt ctgatgtttc taacaggcca
     2281 ggattaactg cgaatcgttc tagctccctg cttgcccata ctatatgttt taatttatat
     2341 tttttctttc cccctggcct taaccgaatt ttttcccatc gcgatctaat tctcccccgc
     2401 ttaatactga cgctctcgca cccatctctc tccttctagc ctccgctagt caaaattttt
     2461 ggcgtactca ccagtcgccg cccctcgcct cttgccgtgc gcgcttcagc aagccgagtc
     2521 ctgcgtcgag agagctcctc tggtttccct ttcgctttca agtccctgtt cgggcgccac
     2581 tgctagagat tttccacact gactaaaagg gtctgaggga tctctagtta ccagagtcac
     2641 acaacagacg ggcacacact acttgaagca ctcaaggcaa gctttattga ggcttaagca
     2701 gtgggttccc tagttagcca gagagctccc aggctcagat ctggtctaac cagagagacc
     2761 cgtttatgta tcgagctagg cacttaaata caatatctct gcaatgcggc aattcagtgg
     2821 ttcgtccaat ccatgtcaga cccgtctgtt gccttcctaa taaggcacga tcgtaccacc
     2881 ttacttccac caatcggcat gcacggtgct ttttctctcc ttgtaaggca tgttgctaac
     2941 tcatcgttac catgttgcaa gactacaaga gtattgcata agactacatt aagcttgcag
     3001 ctccagcttt tgttcccttt agtgagggtt aattgcgcgc ttggcgtaat catggtcata
     3061 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     3121 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     3181 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     3241 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     3301 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     3361 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     3421 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     3481 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     3541 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     3601 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     3661 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     3721 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     3781 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     3841 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     3901 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     3961 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     4021 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     4081 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     4141 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     4201 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     4261 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     4321 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     4381 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     4441 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     4501 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     4561 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     4621 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     4681 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     4741 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     4801 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     4861 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     4921 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     4981 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     5041 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     5101 aagcatttat cagggttatt gtctcatgag cggatacata tttgaaaggc ctccaaaaaa
     5161 gcctcctcac tacttctgga atagctcaga ggccgaggcg gcctcggcct ctgcataaat
     5221 aaaaaaaatt agtcagccat ggggcggaga atgggcggaa ctgggcggag ttaggggcgg
     5281 gatgggcgga gttaggggcg ggatagctag agccagacat gataagatac attgatgagt
     5341 ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg
     5401 ctattgcttt atttgtaacc attataagct gcaataaaca agttcctctc actctctgat
     5461 attcatttct ttgcaagtta taaatactga ataataagat gacatgaact actactgcta
     5521 gagattttcc acactgacta aaagggtctg agggatctct agttaccaga gtcacacaac
     5581 agacgggcac acactacttg aagcactcaa ggcaagcttt attgaggctt aagcagtggg
     5641 ttccctagtt agccagagag ctcccaggct cagatctggt ctaaccagag agacccagta
     5701 caagcaaaaa gcagatcttg tcttcgttgg gagtgaatta gcccttccag tccccccttt
     5761 tcttttaaaa agtggctaag atctacagct gccttgtaag tcattggtct taaaggtacc
     5821 tctagtccgg acgccgaggc gaaacaggcg gggaggcggc ccaaagggag atccgactcg
     5881 tctgagggcg aaggcgaaga cgcggaagag gccgcagagc cggcagcagg ccgcgggaag
     5941 gaaggtccgc tggattgagg gccgaaggga cgtagcagaa ggacgtcccg cgcagaatcc
     6001 aggtggcaac acaggcgagc agccatggaa aggacgtcag cttccccgac aacaccacgg
     6061 aattgtcagt gcccaacagc cgagcccctg tccagcagcg ggcaaggcag gcggcgatga
     6121 gttccgccgt ggcaataggg agggggaaag cgaaagtccc ggaaaggagc tgacaggtgg
     6181 tggcaatgcc ccaaccagtg ggggttgcgt cagcaaacac agtgcacacc acgccacgtt
     6241 gcctgacaac gggccacaac tcctcataaa gagacagcaa ccaggattta tacaaggagg
     6301 agaaaatgaa agccatacgg gaagcaatag catgatacaa aggcattaaa gcagcgtatc
     6361 cacatagcgt aaaaggagca acatagttaa gaatacgata tcttggatcg atccttactt
     6421 agttacccgg ggagcatgtc aaggtcaaaa tcgtcaagag cgtcagcagg cagcatatca
     6481 aggtcaaagt cgtcaagggc atcggctggg agcatgtcta agtcaaaatc gtcaagggcg
     6541 tcggtcggcc cgccgctttc gcactttagc tgtttctcca ggccacatat gattagttcc
     6601 aggccgaaaa ggaaggcagg ttcggctccc tgccggtcga acagctcaat tgcttgtctc
     6661 agaagtgggg gcatagaatc ggtggtaggt gtctctcttt cctcttttgc tacttgatgc
     6721 tcctgttcct ccaatacgca gcccagtgta aagtggccca cggcggacag agcgtacagt
     6781 gcgttctcca gggagaagcc ttgctgacac aggaacgcga gctgattttc cagggtttcg
     6841 tactgtttct ctgttgggcg ggtgccgaga tgcactttag ccccgtcgcg atgtgagagg
     6901 agagcacagc ggtatgactt ggcgttgttc cgcagaaagt cttgccatga ctcgccttcc
     6961 agggggcaga agtgggtatg atgcctgtcc agcatctcga ttggcagggc atcgagcagg
     7021 gcccgcttgt tcttcacgtg ccagtacagg gtaggctgct caactcccag cttttgagcg
     7081 agtttccttg tcgtcaggcc ttcgataccg acaccattga gtaattccag agctccgttt
     7141 atgactttgc tcttgtccag tctagacatt ggaccagggt tttcttcaac atcaccacaa
     7201 gtgaggagag aacctctacc ttcggcaccg ggcttgcggg tcatgcacca ggtgcgcggt
     7261 ccttcgggca cctcgacgtc ggcggtgacg gtgaagccga gccgctcgta gaaggggagg
     7321 ttgcggggcg cggaggtctc caggaaggcg ggcaccccgg cgcgctcggc cgcctccact
     7381 ccggggagca cgacggcgct gcccagaccc ttgccctggt ggtcgggcga gacgccgacg
     7441 gtggccagga accacgcggg ctccttgggc cggtgcggcg ccaggaggcc ttccatctgt
     7501 tgctgcgcgg ccagccggga accgctcaac tcggccatgc gcgggccgat ctcggcgaac
     7561 accgcccccg cttcgacgct ctccggcgtg gtccagaccg ccaccgcggc gccgtcgtcc
     7621 gcgacccaca ccttgccgat gtcgagcccg acgcgcgtga ggaagagttc ttgcagctcg
     7681 gtgacccgct cgatgtggcg gtccggatcg acggtgtggc gcgtggcggg gtagtcggcg
     7741 aacgcggcgg cgagggtgcg tacggccctg gggacgtcgt cgcgggtggc gaggcgcacc
     7801 gtgggcttgt actcggtcat ggtgaattgc tggggagaga ggtcggtgat tcggtcaacg
     7861 agggagccga ctgccgacgt gcgctccgga ggcttgcaga atgcggaaca ccgcgcgggc
     7921 aggaacaggg cccacactac cgccccacac cccgcctccc gcaccgcccc ttcccggccg
     7981 ctgctctcgg cgcgccctgc tgagcagccg ctattggcca cagcccatcg cggtcggcgc
     8041 gctgccattg ctccctggcg ctgtccgtct gcgagggtac tagtgagacg tgcggcttcc
     8101 gtttgtcacg tccggcacgc cgcgaaccgc aaggaacctt cccgacttag gggcggagca
     8161 ggaagcgtcg ccggggggcc cacaagggta gcggcgaaga tccgggtgac gctgcgaacg
     8221 gacgtgaaga atgtgcgaga cccagggtcg gcgccgctgc gtttcccgga accacgccca
     8281 gagcagccgc gtccctgcgc aaacccaggg ctgccttgga aaaggcgcaa ccccaacccc
     8341 ggatccttag tggtggtggt ggtggtggac cggttcatta ctaaaccact ttgtacaaga
     8401 aagctgggtc tagatatctc gacggtatcg ataagcttga taattcttaa ttaattcatt
     8461 acttgtacag ctcgtccatg ccgagagtga tcccggcggc ggtcacgaac tccagcagga
     8521 ccatgtgatc gcgcttctcg ttggggtctt tgctcagggc ggactgggtg ctcaggtagt
     8581 ggttgtcggg cagcagcacg gggccgtcgc cgatgggggt gttctgctgg tagtggtcgg
     8641 cgagctgcac gctgccgtcc tcgatgttgt ggcggatctt gaagttcgcc ttgatgccgt
     8701 tcttctgctt gtcggccatg atatagacgt tgtggctgtt gtagttgtac tccagcttgt
     8761 gccccaggat gttgccgtcc tccttgaagt cgatgccctt cagctcgatg cggttcacca
     8821 gggtgtcgcc ctcgaacttc acctcggcgc gggtcttgta gttgccgtcg tccttgaaga
     8881 agatggtgcg ctcctggacg tagccttcgg gcatggcgga cttgaagaag tcgtgctgct
     8941 tcatgtggtc ggggtagcgg ctgaagcact gcacgccgta ggtcagggtg gtcacgaggg
     9001 tgggccaggg cacgggcagc ttgccggtgg tgcagatgaa cttcagggtc agcttgccgt
     9061 aggtggcatc gccctcgccc tcgccagaca cgctgaactt gtggccgttt acgtcgccgt
     9121 ccagctcgac caggatgggc accaccccgg tgaacagctc ctcgcccttg ctcaccatgg
     9181 ttgtggccat attatcatcg tgtttttcaa aggaaaacca cgtccccgtg gttcgggggg
     9241 cctagacgtt tttttaacct cgactaaaca catgtaaagc atgtgcaccg aggccccaga
     9301 tcagatccca tacaatgggg taccttctgg gcatccttca gccccttgtt gaatacgctt
     9361 gaggagagcc atttgactct ttccacaact atccaactca caacgtggca ctggggttgt
     9421 gccgcctttg caggtgtatc ttatacacgt ggcttttggc cgcagaggca cctgtcgcca
     9481 ggtggggggt tccgctgcct gcaaagggtc gctacagacg ttgtttgtct tcaagaagct
     9541 tccagaggaa ctgcttcctt cacgacattc aacagacctt gcattccttt ggcgagaggg
     9601 gaaagacccc taggaatgct cgtcaagaag acagggccag gtttccgggc cctcacattg
     9661 ccaaaagacg gcaatatggt ggaaaataac atatagacaa acgcacaccg gccttattcc
     9721 aagcggcttc ggccagtaac gttagggggg gggggcggaa ttcctgcagc ccgggggaat
     9781 caccactttg tacaagaaag ctgggtctag atatctcgag tgcggccgcg aattaccttt
     9841 tgaatagact ttaattgaga gcaaagtgaa aaatgcaggt tacaatttta agtgttatca
     9901 tcaatatctt gtaagatcaa cacccagtga tgcatctagt acttatacag tcctaatttt
     9961 agttgtcaaa gagcaaatca ttatcagaca tatatttaca cagtaatttc aagcaacggt
    10021 aaataataaa ctccctccct taaaaaacaa aacacaacac aacacacaca cacacacaca
    10081 cacacacgac catctttaaa caatggattt cctcacccag taagtctcat ttttttcaca
    10141 tatacttgta gacccaaagg tccttaagtt acttaaaaac agtcttatca gtggagatcc
    10201 tggaccatcc cctattttct tttaaagtcg ctttcatttt tacaacttga agccatatac
    10261 cagcatggtt tttatacact aaggacactc ccagtgatga aaatgaattc ccctccgttt
    10321 gtgcaaggag gtatgtacat ctataaagac acatatgatt accactcaaa agagtcaaaa
    10381 acatacacaa aaaaatctca gatcttcata gtttcttaag agcagtgtgc cagtgttcac
    10441 attgaattaa ctgaatggta aaattctttt agatttctat acagaggtac tggttagttc
    10501 tgattctttc ttcagctgct tgaaatgcat gagctttaaa agttgcctgg cagaactaca
    10561 tcctgctttc caggttagca gcctggctga cctcgggaat gttagacaag attcaccccc
    10621 gatgccttgc tctctgattt atttcttgct gttgctgtta gtaaatggaa accatgacaa
    10681 agtttacatt agcagacaca tacacatgcc ctggcctata aatattccat gatagaaaat
    10741 ggtacaataa ttccatcccc agcgaaaacg agcataaaaa aagaagggaa gggtcagggg
    10801 gacatgatca gctatggctc ttcttacagt agaaaatcca caccaaatgg caatgggctt
    10861 ttaactaacc agacattgtt agctgcttct ccagggcctg tgagtcaact aaaagtaggc
    10921 agggcagaga ccaactcttc caggcacacc tggtagtttc cagaagcacc ggtatcttgt
    10981 cttggaagtt ggatgaagaa gatcaactga agtttccttt ttagtgagga ggagtggaga
    11041 gtcagacttg aaagcagttc acacactgtg ctgcctggga cactgaacgg tccccgaggg
    11101 agacccctca aagcgccagc tggagtttgg tcatgtttct ctgatgcatg ttgtgatggc
    11161 ggactaattc atctgaccgg gcaaactttt tctgacaact tggccaccga cagctgaagg
    11221 gcttttcacc tgtatgagtc ctggtgtggg tcttcaggtg gtcggaccgg gagaactttc
    11281 gctgacaagt ttcatacact ggaatggttt cacacctgta tgtctccttt ggtgtctttt
    11341 gagctggtct gaacgagaaa accttcgttc acagtccttg aagtcacact gg
//
