LOCUS       sequence-43399-e         657 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 657)
  AUTHORS   White EA, Sowa ME, Tan MJ, Jeudy S, Hayes SD, Santha S, Munger K, 
            Harper JW, Howley PM
  TITLE     Systematic identification of interactions between host cell proteins
            and E7 oncoproteins from diverse human papillomaviruses.
  JOURNAL   Proc Natl Acad Sci U S A. 2012 Jan 9.
  PUBMED    22232672
REFERENCE   2  (bases 1 to 657)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2012 Jan 9."
COMMENT     Sequence Label: p6640 MSCV-P C-FlagHA 16E7-Kozak
FEATURES             Location/Qualifiers
     source          1..657
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(23..46)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    78..102
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(397..421)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(450..469)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(451..469)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(497..513)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(529..551)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
ORIGIN
        1 ggggtatcct ccagtcggcc gacttgtcat cgtcgtcctt gtagtcctcg agttcgaacc
       61 gcgggccctc tagatcaacc actttgtaca agaaagttgg tggtttctga gaacagatgg
      121 ggcacacaat tcctagtgtg cccattaaca ggtcttccaa agtacgaatg tctacgtgtg
      181 tgctttgtac gcacaaccga agcgtagagt cacacttgca acaaaaggtt acaatattgt
      241 aatgggctct gtccggttct gcttgtccag ctggaccatc tatttcatcc tcctcctctg
      301 agctgtcatt taattgctca taacagtaga gatcagttgt ctctggttgc aaatctaaca
      361 tatattcatg caatgtaggt gtatctccat gcatggtgcc aacttttttg tacaaacttg
      421 ttgatagctt aactagccag cttgggtctc cctatagtga gtcgtattag atctaattcc
      481 ggcgcctaga gaaggagtga gggctggata aagggaggat cgaggcgggg tcgaacgagg
      541 aggttcaagg gggagagacg gggcggatgg aggaagagga ggcggaggct tanggtgtac
      601 aaagggcttg acccagggag gggggtcaaa agccaaggct tcccaggtca cgatgta
//
