LOCUS       sequence-434072-        4816 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mTGFB1 expression in mammlian cells. pAAV vector. Reporter plasmid..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4816)
  AUTHORS   Baloban M, Kasatkina LA, Verkhusha VV
  TITLE     iLight2: A near-infrared optogenetic tool for gene transcription 
            with low background activation.
  JOURNAL   Protein Sci. 2024 May;33(5):e4993. doi: 10.1002/pro.4993.
  PUBMED    38647395
REFERENCE   2  (bases 1 to 4816)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Sci."; date: "2024-05"; volume: "33(5):e4993. doi"; pages: " 
            10.1002/pro.4993"
COMMENT     Sequence Label: pAAV-G12-mTGFB1
FEATURES             Location/Qualifiers
     source          1..4816
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        174..192
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     174..191
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(1748..1768)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     primer_bind     1950..1969
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     repeat_region   2079..2219
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   2079..2208
                     /label=AAV2 ITR
     rep_origin      2294..2749
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2381..2400)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2591..2612
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(2766..2785)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2885..2907
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(2945..2963)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3031..3135
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3136..3204,3205..3996)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3354..3373)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4167..4755
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4656..4675
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgg gcgaccaaag gtcgcccggc
       61 ctcagtgagc gagcgagcgc gcagagaggg agtggccaac tccatcacta ggggttcctg
      121 cggccgaaga tgctgcacac ttgtgggcta gccttatgta tcatacacat acgatttagg
      181 tgacactata gaactcgagc agctgaagct tcggagtact gtcctccgag cggagtactg
      241 tcctccgagc ggagtactgt cctccgagtc gactctagcg gagtactgtc ctccgagcgg
      301 agtactgtcc tccgagcgga gtactgtcct ccgagtcgac tctagcggag tactgtcctc
      361 cgagcggagt actgtcctcc gagcggagta ctgtcctccg agtcgactct agcggagtac
      421 tgtcctccga gcggagtact gtcctccggc tagtagcgga gactctagag ggtatataat
      481 ggatccccgg gtaccggtcg ccaccatgcc gccctcgggg ctgcggctac tgccgcttct
      541 gctcccactc ccgtggcttc tagtgctgac gcccgggagg ccagccgcgg gactctccac
      601 ctgcaagacc atcgacatgg agctggtgaa acggaagcgc atcgaagcca tccgtggcca
      661 gatcctgtcc aaactaaggc tcgccagtcc cccaagccag ggggaggtac cgcccggccc
      721 gctgcccgag gcggtgctcg ctttgtacaa cagcacccgc gaccgggtgg caggcgagag
      781 cgccgaccca gagccggagc ccgaagcgga ctactatgct aaagaggtca cccgcgtgct
      841 aatggtggac cgcaacaacg ccatctatga gaaaaccaaa gacatctcac acagtatata
      901 tatgttcttc aatacgtcag acattcggga agcagtgccc gaacccccat tgctgtcccg
      961 tgcagagctg cgcttgcaga gattaaaatc aagtgtggag caacatgtgg aactctacca
     1021 gaaatatagc aacaattcct ggcgttacct tggtaaccgg ctgctgaccc ccactgatac
     1081 gcctgagtgg ctgtcttttg acgtcactgg agttgtacgg cagtggctga accaaggaga
     1141 cggaatacag ggctttcgat tcagcgctca ctgctcttgt gacagcaaag ataacaaact
     1201 ccacgtggaa atcaacggga tcagccccaa acgtcggggc gacctgggca ccatccatga
     1261 catgaaccgg cccttcctgc tcctcatggc cacccccctg gaaagggccc agcacctgca
     1321 cagctcacgg caccggagag ccctggatac caactattgc ttcagctcca cagagaagaa
     1381 ctgctgtgtg cggcagctgt acattgactt taggaaggac ctgggttgga agtggatcca
     1441 cgagcccaag ggctaccatg ccaacttctg tctgggaccc tgcccctata tttggagcct
     1501 ggacacacag tacagcaagg tccttgccct ctacaaccaa cacaacccgg gcgcttcggc
     1561 gtcaccgtgc tgcgtgccgc aggctttgga gccactgccc atcgtctact acgtgggtcg
     1621 caagcccaag gtggagcagt tgtccaacat gattgtgcgc tcctgcaagt gcagctgaga
     1681 attccgctcg agataatcaa cctctggatt acaaaatttg tgaaagattg actggtattc
     1741 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg
     1801 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttagttcttg
     1861 ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg
     1921 gcactgacaa ttccgtggtg tttatttgtg aaatttgtga tgctattgct ttatttgtaa
     1981 ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg
     2041 ttcaggggga gatgtgggag gttttttaaa gcggccgcag gaacccctag tgatggagtt
     2101 ggccactccc tctctgcgcg ctcgctcgct cactgaggcc gggcgaccaa aggtcgcccg
     2161 acgcccgggc tttgcccggg cggcctcagt gagcgagcga gcgcgcagct gcctgcaggg
     2221 gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatacgtca
     2281 aagcaaccat agtacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
     2341 cgcagcgtga ccgctacact tgccagcgcc ttagcgcccg ctcctttcgc tttcttccct
     2401 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
     2461 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgattt gggtgatggt
     2521 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
     2581 ttctttaata gtggactctt gttccaaact ggaacaacac tcaactctat ctcgggctat
     2641 tcttttgatt tataagggat tttgccgatt tcggtctatt ggttaaaaaa tgagctgatt
     2701 taacaaaaat ttaacgcgaa ttttaacaaa atattaacgt ttacaatttt atggtgcact
     2761 ctcagtacaa tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc
     2821 gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc
     2881 gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga
     2941 aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag
     3001 acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     3061 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     3121 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
     3181 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
     3241 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
     3301 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
     3361 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
     3421 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
     3481 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
     3541 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     3601 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     3661 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
     3721 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
     3781 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
     3841 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
     3901 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
     3961 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     4021 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     4081 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     4141 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     4201 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     4261 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     4321 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     4381 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     4441 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     4501 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     4561 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     4621 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     4681 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     4741 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     4801 ccttttgctc acatgt
//
