LOCUS       sequence-434256-        8744 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses N-terminally EGFP-tagged IFT70B in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8744)
  AUTHORS   Takei R, Katoh Y, Nakayama K
  TITLE     Robust interaction of IFT70 with IFT52-IFT88 in the IFT-B complex is
            required for ciliogenesis.
  JOURNAL   Biol Open. 2018 Apr 30;7(5). pii: bio.033241. doi: 
            10.1242/bio.033241.
  PUBMED    29654116
REFERENCE   2  (bases 1 to 8744)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biol 
            Open."; date: "2018-04-30"; volume: "7(5). pii: bio.033241. doi"; 
            pages: " 10.1242/bio.033241"
COMMENT     Sequence Label: pCAG-EGFP-C-IFT70B
FEATURES             Location/Qualifiers
     source          1..8744
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        4..383
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        385..662
                     /label=chicken beta-actin promoter
     intron          663..1679
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1603..1625
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1687..1706
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      1744..1753
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1750..1752,1753..1755,1756..2466)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1750..1752,1753..1755,1756..2466)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1795..1816)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2056..2075)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2403..2424
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(4616..4635)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    4681..4736
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(4735..4754)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5097..5113)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5097..5113)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5110..5132)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5121..5137
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5145..5151,5152..5169,5170..5175))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5190..5211
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        5270..5465
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      5316..5451
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    5471..5605
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5508..5527)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5562..5581
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5673..5690)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5844..6432)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5924..5943)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6603..7394,7395..7463))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7226..7245
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7464..7568)
                     /gene="bla"
                     /label=AmpR promoter
     CDS             7880..8683
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7943..7962)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8553..8572
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8690..8738
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
        1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atggtcgagg tgagccccac gttctgcttc actctcccca
      421 tctccccccc ctccccaccc ccaattttgt atttatttat tttttaatta ttttgtgcag
      481 cgatgggggc gggggggggg ggggggcgcg cgccaggcgg ggcggggcgg ggcgaggggc
      541 ggggcggggc gaggcggaga ggtgcggcgg cagccaatca gagcggcgcg ctccgaaagt
      601 ttccttttat ggcgaggcgg cggcggcggc ggccctataa aaagcgaagc gcgcggcggg
      661 cgggagtcgc tgcgcgctgc cttcgccccg tgccccgctc cgccgccgcc tcgcgccgcc
      721 cgccccggct ctgactgacc gcgttactcc cacaggtgag cgggcgggac ggcccttctc
      781 ctccgggctg taattagcgc ttggtttaat gacggcttgt ttcttttctg tggctgcgtg
      841 aaagccttga ggggctccgg gagggccctt tgtgcggggg gagcggctcg gggggtgcgt
      901 gcgtgtgtgt gtgcgtgggg agcgccgcgt gcggctccgc gctgcccggc ggctgtgagc
      961 gctgcgggcg cggcgcgggg ctttgtgcgc tccgcagtgt gcgcgagggg agcgcggccg
     1021 ggggcggtgc cccgcggtgc ggggggggct gcgaggggaa caaaggctgc gtgcggggtg
     1081 tgtgcgtggg ggggtgagca gggggtgtgg gcgcgtcggt cgggctgcaa ccccccctgc
     1141 acccccctcc ccgagttgct gagcacggcc cggcttcggg tgcggggctc cgtacggggc
     1201 gtggcgcggg gctcgccgtg ccgggcgggg ggtggcggca ggtgggggtg ccgggcgggg
     1261 cggggccgcc tcgggccggg gagggctcgg gggaggggcg cggcggcccc cggagcgccg
     1321 gcggctgtcg aggcgcggcg agccgcagcc attgcctttt atggtaatcg tgcgagaggg
     1381 cgcagggact tcctttgtcc caaatctgtg cggagccgaa atctgggagg cgccgccgca
     1441 ccccctctag cgggcgcggg gcgaagcggt gcggcgccgg caggaaggaa atgggcgggg
     1501 agggccttcg tgcgtcgccg cgccgccgtc cccttctccc tctccagcct cggggctgtc
     1561 cgcgggggga cggctgcctt cgggggggac ggggcagggc ggggttcggc ttctggcgtg
     1621 tgaccggcgg ctctagagcc tctgctaacc atgttcatgc cttcttcttt ttcctacagc
     1681 tcctgggcaa cgtgctggtt attgtgctgt ctcatcattt tggcaaagaa tttgctagtt
     1741 ttcgccacca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc
     1801 gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat
     1861 gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc
     1921 tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg ctaccccgac
     1981 cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc
     2041 accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc
     2101 gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc
     2161 ctggggcaca agctggagta caactacaac agccacaacg tctatatcat ggccgacaag
     2221 cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg
     2281 cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc
     2341 gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga gaagcgcgat
     2401 cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg
     2461 tacaagtccg gactcagatc catggctggc ctgagcggcg cgcagatccc cgacggggag
     2521 ttcaccgcgg tcgtgtaccg cctcatccgc aatgcacgct acgccgaggc ggtgcagctg
     2581 ctgggcggag aactgcagcg gagccctagg agccgcgccg gcctgtcgct gctaggctac
     2641 tgctactacc gcctgcagga gttcgcgctg gcggccgagt gctatgagca gctgggccag
     2701 ctgcacccgg aactggagca gtaccgcctg taccaggccc aggccctata caaggcctgc
     2761 ctttatgcgg aggccacccg ggtcgccttc cttctcctgg ataaccccgc ctaccacagc
     2821 cgggtcctcc acctgcaagc tgctatcaag tacagcgagg gcgatctgcc agggtccagg
     2881 agcctggtag agcagctgcc gagtagggaa gggggagagg aaagtggggg cgagaatgag
     2941 accgatggcc agatcaacct gggttgtttg ctctacaagg agggacagta tgaagctgca
     3001 tgctccaagt tttttgccgc cctgcaggcc tcgggctacc agcctgacct ttcctacaac
     3061 ctggctttgg cctattacag cagccgacag tatgcttcag cactgaagca tatcgctgag
     3121 attattgagc gtggcatccg ccagcaccct gagctaggtg tgggcatgac cactgagggc
     3181 attgatgttc gcagtgttgg caacacctta gtcctccatc agactgctct ggtggaagcc
     3241 ttcaacctta aggcagctat agaataccaa ctgagaaact atgaggcagc tcaagaagcc
     3301 ctcactgaca tgccacccag ggcagaggaa gagttggacc ctgtgaccct acacaaccag
     3361 gcactaatga acatggatgc caggcctaca gaagggtttg aaaagctaca gtttttgctc
     3421 caacagaatc cctttcctcc agagactttt ggcaacctgt tgctgctcta ctgtaaatat
     3481 gagtattttg acctggcagc agatgtcctg gcagaaaatg cccatttgat ttataagttc
     3541 ctcacaccct atctctatga cttcttggac gctgtgatca cttgccagac agctcctgaa
     3601 gaggctttca ttaagcttga tgggctagca gggatgctga ctgaggtcct ccggaaactt
     3661 accatacaag tacaggaagc aagacacaat agagatgatg aagctatcaa aaaggcagtg
     3721 aatgaatatg atgaaaccat ggagaaatac attcctgtgt tgatggctca ggcaaaaatc
     3781 tactggaatc ttgaaaatta tccaatggtg gaaaagatct tccgcaaatc tgtggaattc
     3841 tgtaacgacc atgatgtgtg gaagttgaat gtggctcatg ttctgttcat gcaggaaaac
     3901 aaatacaaag aagccattgg tttctatgaa cccatagtca agaagcatta tgataacatc
     3961 ctgaatgtca gtgctattgt actggctaat ctctgtgttt cctatattat gacaagtcaa
     4021 aatgaagaag cagaggagtt gatgaggaag attgaaaagg aggaagagca gctctcttat
     4081 gatgacccag ataagaaaat gtaccatctc tgcattgtga atttggtgat aggaactctt
     4141 tattgtgcca aaggaaatta tgactttggt atttctcgag ttatcaaaag cttggaacct
     4201 tacaacaaaa agctgggaac agacacctgg tattatgcca aaagatgctt cctgtccttg
     4261 ttagaaaaca tgtcaaaaca cacaatcatg cttcgtgata gtgttattca agaatgtgtc
     4321 cagtttctag aacactgtga acttcatggc agaaacatac ctgctgttat tgaacaaccc
     4381 ctggaagaag aaagaatgca tgttggaaag aatacagtca catatgagtc taggcagtta
     4441 aaagctttga tttatgagat tataggatgg aatatatagg tcgagggtac cgcgggcccg
     4501 ggatccaccg gatctagata actgatcaca attcactcct caggtgcagg ctgcctatca
     4561 gaaggtggtg gctggtgtgg ccaatgccct ggctcacaaa taccactgag atcgatcttt
     4621 ttccctctgc caaaaattat ggggacatca tgaagcccct tgagcatctg acttctggct
     4681 aataaaggaa atttattttc attgcaatag tgtgttggaa ttttttgtgt ctctcactcg
     4741 gaaggacata tgggagggca aatcatttaa aacatcagaa tgagtatttg gtttagagtt
     4801 tggcaacata tgcccatatg ctggctgcca tgaacaaagg ttggctataa agaggtcatc
     4861 agtatatgaa acagccccct gctgtccatt ccttattcca tagaaaagcc ttgacttgag
     4921 gttagatttt ttttatattt tgttttgtgt tatttttttc tttaacatcc ctaaaatttt
     4981 ccttacatgt tttactagcc agatttttcc tcctctcctg actactccca gtcatagctg
     5041 tccctcttct cttatggaga tccctcgacc tgcagcccaa gcttggcgta atcatggtca
     5101 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
     5161 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
     5221 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agcggatccg catctcaatt
     5281 agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt
     5341 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
     5401 cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt
     5461 gcaaaaagct aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
     5521 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
     5581 caatgtatct tatcatgtct ggatccgctg cattaatgaa tcggccaacg cgcggggaga
     5641 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     5701 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     5761 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     5821 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     5881 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     5941 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     6001 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     6061 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     6121 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     6181 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     6241 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     6301 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     6361 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     6421 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     6481 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     6541 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     6601 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     6661 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     6721 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     6781 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     6841 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     6901 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     6961 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     7021 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     7081 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     7141 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     7201 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     7261 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     7321 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     7381 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     7441 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     7501 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     7561 gttccgcgca catttccccg aaaagtgcca cctgggtcga gcagtgtggt tttcaagagg
     7621 aagcaaaaag cctctccacc caggcctgga atgtttccac ccaatgtcga gcagtgtggt
     7681 tttgcaagag gaagcaaaaa gcctctccac ccaggcctgg aatgtttcca cccaatgtcg
     7741 agcaaacccc gcccagcgtc ttgtcattgg cgaattaatt cgaacacgca gatgcagtcg
     7801 gggcggcgcg gtcccaggtc cacttcgcat attaaggtga cgcgtgtggc ctcgaacacc
     7861 gagcgaccct gcagccaata tgggatcggc cattgaacaa gatggattgc acgcaggttc
     7921 tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg
     7981 ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac
     8041 cgacctgtcc ggtgccctga atgaactgca ggacgaggca gcgcggctat cgtggctggc
     8101 cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg
     8161 gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga
     8221 gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg
     8281 cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg
     8341 tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt
     8401 cgccaggctc aaggcgcgca tgcccgacgg cgatgatctc gtcgtgaccc atggcgatgc
     8461 ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg
     8521 gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga
     8581 gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc
     8641 gcagcgcatc gccttctatc gccttcttga cgagttcttc tgaggggatc ggcaataaaa
     8701 agacagaata aaacgcacgg gtgttgggtc gtttgttcgg atcc
//
