LOCUS       sequence-434563-        4103 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway-compatible Entry vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4103)
  AUTHORS   Weller B, Lin CW, Pogoutse O, Sauer M, Marin-de la Rosa N, Strobel 
            A, Young V, Knapp JJ, Rayhan A, Falter C, Kim DK, Roth FP, 
            Falter-Braun P
  TITLE     A resource of human coronavirus protein-coding sequences in a 
            flexible, multipurpose Gateway Entry clone collection.
  JOURNAL   G3 (Bethesda). 2023 Jul 5;13(7):jkad105. doi: 
            10.1093/g3journal/jkad105.
  PUBMED    37267226
REFERENCE   2  (bases 1 to 4103)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda)."; date: "2023-07-5"; volume: "13(7):jkad105. doi"; 
            pages: " 10.1093/g3journal/jkad105"
COMMENT     Sequence Label: pEntry_SARS-CoV-2-Nucleocapsid
FEATURES             Location/Qualifiers
     source          1..4103
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1414..1513)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1530..1549)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1531..1549)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1554..1570)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1554..1570)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2001..2792
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2885..3473
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3374..3393
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3627..3644
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3803..3830)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3922..4008)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     4057..4079
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4071..4088
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4072..4088
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcagaag ggccgtcaag gccaggatcc
      121 atgggatcct ctgataatgg accccaaaat cagcgaaatg caccccgcat tacgtttggt
      181 ggaccctcag attcaactgg cagtaaccag aatggagaac gcagtggggc gcgatcaaaa
      241 caacgtcggc cccaaggttt acccaataat actgcgtctt ggttcaccgc tctcactcaa
      301 catggcaagg aagaccttaa attccctcga ggacaaggcg ttccaattaa caccaatagc
      361 agtccagatg accaaattgg ctactaccga agagctacca gacgaattcg tggtggtgac
      421 ggtaaaatga aagatctcag tccaagatgg tatttctact acctaggaac tgggccagaa
      481 gctggacttc cctatggtgc taacaaagac ggcatcatat gggttgcaac tgagggagcc
      541 ttgaatacac caaaagatca cattggcacc cgcaatcctg ctaacaatgc tgcaatcgtg
      601 ctacaacttc ctcaaggaac aacattgcca aaaggcttct acgcagaagg gagcagaggc
      661 ggcagtcaag cctcttctcg ttcctcatca cgtagtcgca acagttcaag aaattcaact
      721 ccaggcagca gtaggggaac ttctcctgct agaatggctg gcaatggcgg tgatgctgct
      781 cttgctttgc tgctgcttga cagattgaac cagcttgaga gcaaaatgtc tggtaaaggc
      841 caacaacaac aaggccaaac tgtcactaag aaatctgctg ctgaggcttc taagaagcct
      901 cggcaaaaac gtactgccac taaagcatac aatgtaacac aagctttcgg cagacgtggt
      961 ccagaacaaa cccaaggaaa ttttggggac caggaactaa tcagacaagg aactgattac
     1021 aaacattggc cgcaaattgc acaatttgcc cccagcgctt cagcgttctt cggaatgtcg
     1081 cgcattggca tggaagtcac accttcggga acgtggttga cctacacagg tgccatcaaa
     1141 ttggatgaca aagatccaaa tttcaaagat caagtcattt tgctgaataa gcatattgac
     1201 gcatacaaaa cattcccacc aacagagcct aaaaaggaca aaaagaagaa ggctgatgaa
     1261 actcaagcct taccgcagag acagaagaaa cagcaaactg tgactcttct tcctgctgca
     1321 gatttggatg atttctccaa acaattgcaa caatccatga gcagtgctga ctcaactcag
     1381 gcccttaatt aagacgcgat cgcggcctca tgggcccagc tttcttgtac aaagttggca
     1441 ttataaaaaa taattgctca tcaatttgtt gcaacgaaca ggtcactatc agtcaaaata
     1501 aaatcattat ttgccatcca gctgatatcc cctatagtga gtcgtattac atggtcatag
     1561 ctgtttcctg gcagctctgg cccgtgtctc aaaatctctg atgttacatt gcacaagata
     1621 aaaatatatc atcatgcctc ctctagacca gccaggacag aaatgcctcg acttcgctgc
     1681 tacccaaggt tgccgggtga cgcacaccgt ggaaacggat gaaggcacga acccagtgga
     1741 cataagcctg ttcggttcgt aagctgtaat gcaagtagcg tatgcgctca cgcaactggt
     1801 ccagaacctt gaccgaacgc agcggtggta acggcgcagt ggcggttttc atggcttgtt
     1861 atgactgttt ttttggggta cagtctatgc ctcgggcatc caagcagcaa gcgcgttacg
     1921 ccgtgggtcg atgtttgatg ttatggagca gcaacgatgt tacgcagcag ggcagtcgcc
     1981 ctaaaacaaa gttaaacatt atgagggaag cggtgatcgc cgaagtatcg actcaactat
     2041 cagaggtagt tggcgtcatc gagcgccatc tcgaaccgac gttgctggcc gtacatttgt
     2101 acggctccgc agtggatggc ggcctgaagc cacacagtga tattgatttg ctggttacgg
     2161 tgaccgtaag gcttgatgaa acaacgcggc gagctttgat caacgacctt ttggaaactt
     2221 cggcttcccc tggagagagc gagattctcc gcgctgtaga agtcaccatt gttgtgcacg
     2281 acgacatcat tccgtggcgt tatccagcta agcgcgaact gcaatttgga gaatggcagc
     2341 gcaatgacat tcttgcaggt atcttcgagc cagccacgat cgacattgat ctggctatct
     2401 tgctgacaaa agcaagagaa catagcgttg ccttggtagg tccagcggcg gaggaactct
     2461 ttgatccggt tcctgaacag gatctatttg aggcgctaaa tgaaacctta acgctatgga
     2521 actcgccgcc cgactgggct ggcgatgagc gaaatgtagt gcttacgttg tcccgcattt
     2581 ggtacagcgc agtaaccggc aaaatcgcgc cgaaggatgt cgctgccgac tgggcaatgg
     2641 agcgcctgcc ggcccagtat cagcccgtca tacttgaagc tagacaggct tatcttggac
     2701 aagaagaaga tcgcttggcc tcgcgcgcag atcagttgga agaatttgtc cactacgtga
     2761 aaggcgagat caccaaggta gtcggcaaat aaccctcgag ccacccatga ccaaaatccc
     2821 ttaacgtgag ttacgcgtcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     2881 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     2941 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     3001 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     3061 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     3121 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     3181 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     3241 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     3301 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     3361 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     3421 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     3481 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     3541 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     3601 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     3661 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
     3721 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatacgcgt accgctagcc
     3781 aggaagagtt tgtagaaacg caaaaaggcc atccgtcagg atggccttct gcttagtttg
     3841 atgcctggca gtttatggcg ggcgtcctgc ccgccaccct ccgggccgtt gcttcacaac
     3901 gttcaaatcc gctcccggcg gatttgtcct actcaggaga gcgttcaccg acaaacaaca
     3961 gataaaacga aaggcccagt cttccgactg agcctttcgt tttatttgat gcctggcagt
     4021 tccctactct cgcgttaacg ctagcatgga tgttttccca gtcacgacgt tgtaaaacga
     4081 cggccagtct taagctcggg ccc
//
