LOCUS       sequence-434564-        6487 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Localization: Membrane Receptor, Excitation: 434, Emission: 474.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6487)
  TITLE     Davidson Cyan Fluorescent Proteins
REFERENCE   2  (bases 1 to 6487)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mTurquoise-TBRII-N-18
FEATURES             Location/Qualifiers
     source          1..6487
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      537..546
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1873..1884
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     regulatory      2367..2376
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2373..2375,2376..2378,2379..3092)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Goedhart et 
                     al., 2010)"
                     /label=mTurquoise
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISDNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2418..2439)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2679..2698)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3026..3047
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    3215..3336
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3252..3271)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3306..3325
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(3343..3798)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3480..3501)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3692..3711
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3825..3929
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3931..4288
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3931..4122
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3954..3974)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      4139..4274
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4323..5117
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             4323..5117
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4377..4396)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4987..5006
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(5305..5324)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5349..5396
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5725..6313
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6214..6233
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcca
      541 ccatgggtcg ggggctgctc aggggcctgt ggccgctgca catcgtcctg tggacgcgta
      601 tcgccagcac gatcccaccg cacgttcaga agtcggatgt ggaaatggag gcccagaaag
      661 atgaaatcat ctgccccagc tgtaatagga ctgcccatcc actgagacat attaataacg
      721 acatgatagt cactgacaac aacggtgcag tcaagtttcc acaactgtgt aaattttgtg
      781 atgtgagatt ttccacctgt gacaaccaga aatcctgcat gagcaactgc agcatcacct
      841 ccatctgtga gaagccacag gaagtctgtg tggctgtatg gagaaagaat gacgagaaca
      901 taacactaga gacagtttgc catgacccca agctccccta ccatgacttt attctggaag
      961 atgctgcttc tccaaagtgc attatgaagg aaaaaaaaaa gcctggtgag actttcttca
     1021 tgtgttcctg tagctctgat gagtgcaatg acaacatcat cttctcagaa gaatataaca
     1081 ccagcaatcc tgacttgttg ctagtcatat ttcaagtgac aggcatcagc ctcctgccac
     1141 cactgggagt tgccatatct gtcatcatca tcttctactg ctaccgcgtt aaccggcagc
     1201 agaagctgag ttcaacctgg gaaaccggca agacgcggaa gctcatggag ttcagcgagc
     1261 actgtgccat catcctggaa gatgaccgct ctgacatcag ctccacgtgt gccaacaaca
     1321 tcaaccacaa cacagagctg ctgcccattg agctggacac cctggtgggg aaaggtcgct
     1381 ttgctgaggt ctataaggcc aagctgaagc agaacacttc agagcagttt gagacagtgg
     1441 cagtcaagat ctttccctat gaggagtatg cctcttggaa gacagagaag gacatcttct
     1501 cagacatcaa tctgaagcat gagaacatac tccagttcct gacggctgag gagcggaaga
     1561 cggagttggg gaaacaatac tggctgatca ccgccttcca cgccaagggc aacctacagg
     1621 agtacctgac gcggcatgtc atcagctggg aggacctgcg caagctgggc agctccctcg
     1681 cccgggggat tgctaacctc cacagtgatc acactccatg tgggaggccc aagatgccca
     1741 tcgtgcacag ggacctcaag agctccaata tcctcgtgaa gaacgaccta acctgctgcc
     1801 tgtgtgactt tgggctttcc ctgcgtctgg accctactct gtctgtggat gacctggcta
     1861 acagtgggca ggtgggaact gcaagataca tggctccaga agtcctagaa tccaggatga
     1921 atttggagaa tgttgagtcc ttcaagcaga ccgatgtcta ctccatggct ctggtgctct
     1981 gggaaatgac atctcgctgt aatgcagtgg gagaagtaaa agattatgag cctccatttg
     2041 gttccaaggt gcgggagcac ccctgtgtcg aaagcatgaa ggacaacgtg ttgagagatc
     2101 gagggcgacc agaaattccc agcttctggc tcaaccacca gggcatccag atggtgtgtg
     2161 agacgttgac tgagtgctgg gaccacgacc cagaggcccg tctcacagcc cagtgtgtgg
     2221 cagaacgctt cagtgagctg gagcatctgg acaggctctc ggggaggagc tgctcggagg
     2281 agaagattcc tgaagacggc tccctaaaca ctaccaaaag cagcggtgga gcaagcgcag
     2341 ccagtggtag cgcggatcca ccggtcgcca ccatggtgag caagggcgag gagctgttca
     2401 ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg
     2461 tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca
     2521 ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgtcc tggggcgtgc
     2581 agtgcttcgc ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc
     2641 ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc
     2701 gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg
     2761 acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac atcagcgaca
     2821 acgtctatat caccgccgac aagcagaaga acggcatcaa ggccaacttc aagatccgcc
     2881 acaacatcga ggacggcggc gtgcagctcg ccgaccacta ccagcagaac acccccatcg
     2941 gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc aagctgagca
     3001 aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga
     3061 tcactctcgg catggacgag ctgtacaagt aaagcggccg cgactctaga tcataatcag
     3121 ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa
     3181 cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg
     3241 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     3301 tagttgtggt ttgtccaaac tcatcaatgt atcttaaggc gtaaattgta agcgttaata
     3361 ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg
     3421 aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc
     3481 cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa
     3541 ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt
     3601 cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac
     3661 ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta
     3721 gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg
     3781 cgccgctaca gggcgcgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     3841 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     3901 tgcttcaata atattgaaaa aggaagagtc ctgaggcgga aagaaccagc tgtggaatgt
     3961 gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     4021 gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag
     4081 tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat
     4141 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     4201 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
     4261 cttttttgga ggcctaggct tttgcaaaga tcgatcaaga gacaggatga ggatcgtttc
     4321 gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat
     4381 tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt
     4441 cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac
     4501 tgcaagacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg
     4561 tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc
     4621 aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa
     4681 tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc
     4741 gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg
     4801 aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg agcatgcccg
     4861 acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa
     4921 atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg
     4981 acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct
     5041 tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc
     5101 ttgacgagtt cttctgagcg ggactctggg gttcgaaatg accgaccaag cgacgcccaa
     5161 cctgccatca cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat
     5221 cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt
     5281 cgcccaccct agggggaggc taactgaaac acggaaggag acaataccgg aaggaacccg
     5341 cgctatgacg gcaataaaaa gacagaataa aacgcacggt gttgggtcgt ttgttcataa
     5401 acgcggggtt cggtcccagg gctggcactc tgtcgatacc ccaccgagac cccattgggg
     5461 ccaatacgcc cgcgtttctt ccttttcccc accccacccc ccaagttcgg gtgaaggccc
     5521 agggctcgca gccaacgtcg gggcggcagg ccctgccata gcctcaggtt actcatatat
     5581 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     5641 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     5701 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     5761 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     5821 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
     5881 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     5941 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     6001 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     6061 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     6121 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     6181 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     6241 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     6301 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     6361 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
     6421 catgcattag ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg
     6481 agttccg
//
