LOCUS       sequence-434589-         670 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses mitochondrially targeted TALE-free DddAtox 1397C-UGI in 
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 670)
  AUTHORS   Castillo SR, Simone BW, Clark KJ, Devaux P, Ekker SC
  TITLE     Unconstrained Precision Mitochondrial Genome Editing with 
            alphaDdCBEs.
  JOURNAL   Hum Gene Ther. 2024 Sep 24. doi: 10.1089/hum.2024.073.
  PUBMED    39212664
REFERENCE   2  (bases 1 to 670)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Hum Gene 
            Ther. 2024 Sep 24. doi: 10.1089/hum.2024.073."
COMMENT     Sequence Label: TALE-free DddAtox 1397C
FEATURES             Location/Qualifiers
     source          1..670
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..78
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MASVLTPLLLRGLTGSARRLPVPRAK"
     CDS             91..156
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             268..519
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
ORIGIN
        1 atggcctccg ttctgacccc gctgctgctg cgtggcctga ccggctccgc tcgtcgtctg
       61 ccagttccgc gtgcgaaaat ccattccctg gactacaaag accatgacgg tgattataaa
      121 gatcatgaca tcgattacaa ggatgacgat gacaagatgg gatccgccat tccagtgaag
      181 cgcggcgcta ccggtgaaac caaagtgttt accggtaaca gcaacagccc gaagagcccg
      241 accaaaggcg gttgctctgg cggctcaact aatctgagcg acatcattga gaaggagact
      301 gggaaacagc tggtcattca ggagtccatc ctgatgctgc ctgaggaggt ggaggaagtg
      361 atcggcaaca agccagagtc tgacatcctg gtgcacaccg cctacgacga gtccacagat
      421 gagaatgtga tgctgctgac ctctgacgcc cccgagtata agccttgggc cctggtcatc
      481 caggattcta acggcgagaa taagatcaag atgctgtgat aaggggtctt tgtcctctgt
      541 actgtctctc tccttgcccc taacccaaaa agcttcattt ttctgtgtag gctgcacaag
      601 agccttgatt gaagatatat tctttctgaa cagtatttaa ggtttccaat aaaatgtaca
      661 cccctcagaa
//
