LOCUS       sequence-434783-        2963 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mCherry fluorescent protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2963)
  AUTHORS   Hieronimus K, Donauer T, Klein J, Hinkel B, Spanle JV, Probst A, 
            Niemeyer J, Kibrom S, Kiefer AM, Schneider L, Husemann B, Bischoff 
            E, Mohring S, Bayer N, Klein D, Engels A, Ziehmer BG, Stiebeta J, 
            Moroka P, Schroda M, Deponte M
  TITLE     A Modular Cloning Toolkit for the production of recombinant proteins
            in Leishmania tarentolae.
  JOURNAL   Microb Cell. 2024 Apr 30;11:128-142. doi: 10.15698/mic2024.04.821. 
            eCollection 2024.
  PUBMED    38799406
REFERENCE   2  (bases 1 to 2963)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microb 
            Cell."; date: "2024-04-30"; volume: "11:128-142. doi"; pages: " 
            10.15698/mic2024.04.821. eCollection 2024"
COMMENT     Sequence Label: pMBS-855
FEATURES             Location/Qualifiers
     source          1..2963
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     39..56
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(886..904)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        972..1076
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1298..2089
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2260..2848
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2749..2768
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
       61 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatcac
      121 tctgtggtct caaggtatgg tgagcaaggg cgaggaggac aacatggcga tcatcaagga
      181 gttcatgcgc ttcaaggtgc acatggaggg cagcgtgaac ggccacgagt tcgagatcga
      241 gggcgagggc gagggccgcc cgtacgaggg cacgcagacg gcgaagctga aggtgacgaa
      301 gggcggcccg ctgccgttcg cgtgggacat cctgagcccg cagttcatgt acggcagcaa
      361 ggcgtacgtg aagcacccgg cggacatccc ggactacctg aagctgagct tcccggaggg
      421 cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc gtggtgacgg tgacgcagga
      481 cagcagcctg caggacggcg agttcatcta caaggtgaag ctgcgcggca cgaacttccc
      541 gagcgacggc ccggtgatgc agaagaaaac gatgggctgg gaggcgagca gcgagcgcat
      601 gtacccggag gacggcgcgc tgaagggcga gatcaagcag cgcctgaagc tgaaggacgg
      661 cggccactac gacgcggagg tcaagacgac gtacaaggcg aagaagccgg tgcagctgcc
      721 gggcgcgtac aacgtgaaca tcaagctgga catcacgagc cacaacgagg actacacgat
      781 cgtggagcag tacgagcgcg cggagggccg ccacagcacg ggcggcatgg acgagctgta
      841 caagggttcg tgagaccacg aagtggctct tcagtggacg aaagggcctc gtgatacgcc
      901 tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc
      961 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     1021 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatgc
     1081 gctcacgcaa ctggtccaga accttgaccg aacgcagcgg tggtaacggc gcagtggcgg
     1141 ttttcatggc ttgttatgac tgtttttttt tggggtacag tctatgcctc gggcatccaa
     1201 gcagcaagcg cgttacgccg tgggtcgatg tttgatgtta tggagcagca acgatgttac
     1261 gcagcagggc agtcgcccta aaacaaagtt aaacatcatg agggaagcgg tgatcgccga
     1321 agtatcgact caactatcag aggtagttgg cgtcatcgag cgccatctcg aaccgacgtt
     1381 gctggccgta catttgtacg gctccgcagt ggatggcggc ctgaagccac acagcgatat
     1441 tgatttgctg gttacggtga ccgtaaggct tgatgaaaca acgcggcgag ctttgatcaa
     1501 cgaccttttg gaaacttcgg cttcccctgg agagagcgag attctccgcg ctgtagaagt
     1561 caccattgtt gtgcacgacg acatcattcc gtggcgttat ccagctaagc gcgaactgca
     1621 atttggagaa tggcagcgca atgacattct tgcaggtatc ttcgagccag ccacgatcga
     1681 cattgatctg gctatcttgc tgacaaaagc aagagaacat agcgttgcct tggtaggtcc
     1741 agcggcggag gaactctttg atccggttcc tgaacaggat ctatttgagg cgctaaatga
     1801 aaccttaacg ctatggaact cgccgcccga ctgggctggc gatgagcgaa atgtagtgct
     1861 tacgttgtcc cgcatttggt acagcgcagt aaccggcaaa atcgcgccga aggatgtcgc
     1921 tgccgactgg gcaatggagc gcctgccggc ccagtatcag cccgtcatac ttgaagctag
     1981 acaggcttat cttggacaag aagaagatcg cttggcctcg cgcgcagatc agttggaaga
     2041 atttgtccat tacgtaaaag gcgagatcac caaggtagtc ggcaaataac tgtcagacca
     2101 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     2161 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     2221 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     2281 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     2341 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     2401 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     2461 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     2521 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     2581 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     2641 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     2701 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     2761 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     2821 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     2881 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     2941 taaccgtatt accgcctttg agt
//
