LOCUS       sequence-43486-e        9830 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9830)
  AUTHORS   Lujan E, Chanda S, Ahlenius H, Sudhof TC, Wernig M
  TITLE     Direct conversion of mouse fibroblasts to self-renewing, tripotent 
            neural precursor cells.
  JOURNAL   Proc Natl Acad Sci U S A. 2012 Feb 14;109(7):2527-32. Epub 2012 Jan 
            30.
  PUBMED    22308465
REFERENCE   2  (bases 1 to 9830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2012-02-14"; volume: "109(7)"; pages: 
            "2527-32. Epub 2012 Jan 30"
COMMENT     Sequence Label: Tet-O-FUW-FoxG1
FEATURES             Location/Qualifiers
     source          1..9830
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             145..174
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     misc_feature    1477..2065
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1530..1550)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1948..1959)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(2068..2084)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2069..2085)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             2590..2770
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(2796..2813)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2802..3026
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3072..3500
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3159..3178)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3369..3390
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3509..3529)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3514..3843
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3694..3829
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     3756..3775
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        3891..3938
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             3957..4331
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    4461..4582
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4498..4517)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4552..4571
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4631..4647)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4631..4647)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4644..4666)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4655..4671
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4679..4685,4686..4703,4704..4709))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4724..4745
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4862..4879)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5033..5621)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5113..5132)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5792..6583,6584..6652))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6415..6434
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6653..6757)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(6832..6851)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        7023..7402
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7404..7602
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     7557..7577
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             7620..7800
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7847..7972
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8465..8698
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8883..8927
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9225..9342
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(9396..9414,9415..9437,9438..9456,9457..9479,9480..9498,
                     9499..9521,9522..9540,9541..9563,9564..9582,9583..9605,
                     9606..9624,9625..9647,9648..9666)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        9699..9736
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     9733..9757
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 aattcgatgc tggacatggg agataggaaa gaggtgaaaa tgattcccaa gtcctcgttc
       61 agcatcaaca gcctggtccc cgaggccgtc cagaacgaca accaccacgc gagccacggc
      121 caccacaaca gccaccaccc ccagcatcac catcatcatc accaccacca ccacccgccg
      181 ccgcccgcgc cccagccgcc tccaccgccg ccccagcagc agcagcagca gccgcccccc
      241 gccccgcagc ccccgcaggc gcgcggcgcc ccagcagccg acgacgacaa gggtccccag
      301 ccgctcctgc tcccgccctc caccgccctg gacggggcca aggctgacgc acttggagcc
      361 aaaggcgagc cgggcggcgg gccggcggag ctggcgcccg tcgggccgga cgagaaggag
      421 aagggcgcgg gcgccggggg ggaggagaag aaaggggcgg gcgagggcgg caaggacggg
      481 gaggggggca aggagggcga caagaagaac ggcaagtacg agaagccgcc gttcagctac
      541 aacgcgctca tcatgatggc catcaggcag agtcccgaga agcgcctgac gctcaatggc
      601 atctatgagt tcatcatgaa gaacttcccc tactaccgcg agaacaagca gggctggcag
      661 aactccatcc gccacaacct gtccctcaac aagtgcttcg tgaaggtacc gcgccactac
      721 gacgacccgg gcaagggcaa ctactggatg ctcgacccgt cgagcgacga cgtgttcatc
      781 ggcggcacga ccggcaagct gcggcgccgc tccaccacgt ctcgggccaa gctggccttt
      841 aagcgcgggg cgcgcctcac ctccaccggc ctcaccttca tggaccgcgc cggctccctc
      901 tactggccca tgtcgccctt cctgtccctg caccaccccc gcgccagcag cactttgagt
      961 tacaacggga ccacgtcggc ctaccccagc caccccatgc cctacagctc cgtgttgact
     1021 caaaactcgc tgggcaacaa ccactccttc tccaccgcca acgggctgag tgtggaccgg
     1081 ctggtcaacg gggagatccc gtacgccacg caccacctca cggccgctgc gctcgccgcc
     1141 tcggtgccct gcggcctgtc ggtgccctgc tccgggacct actccctcaa cccctgctcc
     1201 gtcaacctgc tcgcgggcca gaccagttac tttttccccc acgtcccgca cccgtcaatg
     1261 acttcgcaga ccagcacgtc catgagcgcc cgggccgcgt cctcctctac gtcgccgcag
     1321 gccccctcga ccctgccctg tgagtcttta agaccctctt tgccaagttt tacgacagga
     1381 ctgtccgggg gactgtctga ttatttcaca catcaaaatc aggggtcttc ttccaaccct
     1441 ttaatacatt aagaattcga tatcaagctt atcgataatc aacctctgga ttacaaaatt
     1501 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct
     1561 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg
     1621 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc
     1681 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt
     1741 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc
     1801 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg
     1861 ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg
     1921 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc
     1981 ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg
     2041 atctcccttt gggccgcctc cccgcatcga taccgtcgac ctcgagacct agaaaaacat
     2101 ggagcaatca caagtagcaa tacagcagct accaatgctg attgtgcctg gctagaagca
     2161 caagaggagg aggaggtggg ttttccagtc acacctcagg tacctttaag accaatgact
     2221 tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta
     2281 attcactccc aacgaagaca agatatcctt gatctgtgga tctaccacac acaaggctac
     2341 ttccctgatt ggcagaacta cacaccaggg ccagggatca gatatccact gacctttgga
     2401 tggtgctaca agctagtacc agttgagcaa gagaaggtag aagaagccaa tgaaggagag
     2461 aacacccgct tgttacaccc tgtgagcctg catgggatgg atgacccgga gagagaagta
     2521 ttagagtgga ggtttgacag ccgcctagca tttcatcaca tggcccgaga gctgcatccg
     2581 gactgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
     2641 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
     2701 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
     2761 atctctagca gggcccgttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca
     2821 gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac
     2881 tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat
     2941 tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca
     3001 tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag
     3061 ggggtatccc cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
     3121 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
     3181 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
     3241 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
     3301 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
     3361 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
     3421 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
     3481 acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc
     3541 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg
     3601 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     3661 tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
     3721 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
     3781 tctgcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc
     3841 aaaaagctcc cgggagcttg tatatccatt ttcggatctg atcagcacgt gttgacaatt
     3901 aatcatcggc atagtatatc ggcatagtat aatacgacaa ggtgaggaac taaaccatgg
     3961 ccaagttgac cagtgccgtt ccggtgctca ccgcgcgcga cgtcgccgga gcggtcgagt
     4021 tctggaccga ccggctcggg ttctcccggg acttcgtgga ggacgacttc gccggtgtgg
     4081 tccgggacga cgtgaccctg ttcatcagcg cggtccagga ccaggtggtg ccggacaaca
     4141 ccctggcctg ggtgtgggtg cgcggcctgg acgagctgta cgccgagtgg tcggaggtcg
     4201 tgtccacgaa cttccgggac gcctccgggc cggccatgac cgagatcggc gagcagccgt
     4261 gggggcggga gttcgccctg cgcgacccgg ccggcaactg cgtgcacttc gtggccgagg
     4321 agcaggactg acacgtgcta cgagatttcg attccaccgc cgccttctat gaaaggttgg
     4381 gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc
     4441 tggagttctt cgcccacccc aacttgttta ttgcagctta taatggttac aaataaagca
     4501 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     4561 ccaaactcat caatgtatct tatcatgtct gtataccgtc gacctctagc tagagcttgg
     4621 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
     4681 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
     4741 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
     4801 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
     4861 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     4921 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     4981 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     5041 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     5101 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     5161 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     5221 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     5281 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     5341 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     5401 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     5461 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     5521 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     5581 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     5641 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     5701 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     5761 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     5821 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     5881 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     5941 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     6001 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     6061 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     6121 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     6181 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     6241 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     6301 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     6361 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     6421 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     6481 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     6541 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     6601 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     6661 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     6721 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
     6781 ctgacgtcga cggatcggga gatctcccga tcccctatgg tgcactctca gtacaatctg
     6841 ctctgatgcc gcatagttaa gccagtatct gctccctgct tgtgtgttgg aggtcgctga
     6901 gtagtgcgcg agcaaaattt aagctacaac aaggcaaggc ttgaccgaca attgcatgaa
     6961 gaatctgctt agggttaggc gttttgcgct gcttcgcgat gtacgggcca gatatacgcg
     7021 ttgacattga ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag
     7081 cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc
     7141 caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg
     7201 gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca
     7261 tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc
     7321 ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt
     7381 attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata
     7441 gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt
     7501 ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca
     7561 aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agcgcgtttt gcctgtactg
     7621 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
     7681 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
     7741 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
     7801 gtggcgcccg aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag
     7861 gactcggctt gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc
     7921 aaaaattttg actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa
     7981 gcgggggaga attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa
     8041 atataaatta aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc
     8101 tggcctgtta gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct
     8161 tcagacagga tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt
     8221 gcatcaaagg atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca
     8281 aaacaaaagt aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag
     8341 atatgaggga caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat
     8401 taggagtagc acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg
     8461 gaataggagc tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt
     8521 caatgacgct gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca
     8581 atttgctgag ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca
     8641 agcagctcca ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg
     8701 ggatttgggg ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt
     8761 ggagtaataa atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag
     8821 aaattaacaa ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag
     8881 aaaagaatga acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta
     8941 acataacaaa ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag
     9001 gtttaagaat agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac
     9061 cattatcgtt tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag
     9121 aagaagaagg tggagagaga gacagagaca gatccattcg attagtgaac ggatcggcac
     9181 tgcgtgcgcc aattctgcag acaaatggca gtattcatcc acaattttaa aagaaaaggg
     9241 gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa
     9301 actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttcgggttta ttacagggac
     9361 agcagagatc cagtttggtt actcgagttt accactccct atcagtgata gagaaaagtg
     9421 aaagtcgagt ttaccactcc ctatcagtga tagagaaaag tgaaagtcga gtttaccact
     9481 ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca gtgatagaga
     9541 aaagtgaaag tcgagtttac cactccctat cagtgataga gaaaagtgaa agtcgagttt
     9601 accactccct atcagtgata gagaaaagtg aaagtcgagt ttaccactcc ctatcagtga
     9661 tagagaaaag tgaaagtcga gctcggtacc cgggtcgagt aggcgtgtac ggtgggaggc
     9721 ctatataagc agagctcgtt tagtgaaccg tcagatcgcc tggagacgcc atccacgctg
     9781 ttttgacctc catagaagac accgggaccg atccagcctc cgcggccccg
//
