LOCUS       sequence-434861-        2433 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  8xHis-SP20-3xHA-tag for protein detection and purification.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2433)
  AUTHORS   Hieronimus K, Donauer T, Klein J, Hinkel B, Spanle JV, Probst A, 
            Niemeyer J, Kibrom S, Kiefer AM, Schneider L, Husemann B, Bischoff 
            E, Mohring S, Bayer N, Klein D, Engels A, Ziehmer BG, Stiebeta J, 
            Moroka P, Schroda M, Deponte M
  TITLE     A Modular Cloning Toolkit for the production of recombinant proteins
            in Leishmania tarentolae.
  JOURNAL   Microb Cell. 2024 Apr 30;11:128-142. doi: 10.15698/mic2024.04.821. 
            eCollection 2024.
  PUBMED    38799406
REFERENCE   2  (bases 1 to 2433)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microb 
            Cell."; date: "2024-04-30"; volume: "11:128-142. doi"; pages: " 
            10.15698/mic2024.04.821. eCollection 2024"
COMMENT     Sequence Label: pMBS-1022
FEATURES             Location/Qualifiers
     source          1..2433
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             55..78
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             205..231
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(274..292)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        360..464
                     /gene="bla"
                     /label=AmpR promoter
     CDS             686..1477
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1648..2236
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2137..2156
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2390..2407
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 accgcctctc cccgcgcgtt ggccgattca ttaatcactc tgtggtctca ttcgcaccac
       61 caccaccacc accaccactc gccctcgccc agcccgagcc cttcgccttc gccctcgccg
      121 tcgccatccc cgagccccag cccctccccc agcccgagcc cttcgccttc gccctcgccg
      181 tcgccatccc cgagcccctc tggttacccc tacgacgtgc ccgactacgc ttaagctttg
      241 agaccacgaa gtggctcttc agtggacgaa agggcctcgt gatacgccta tttttatagg
      301 ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc
      361 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
      421 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgcgc tcacgcaact
      481 ggtccagaac cttgaccgaa cgcagcggtg gtaacggcgc agtggcggtt ttcatggctt
      541 gttatgactg tttttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg
      601 ttacgccgtg ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag
      661 tcgccctaaa acaaagttaa acatcatgag ggaagcggtg atcgccgaag tatcgactca
      721 actatcagag gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca
      781 tttgtacggc tccgcagtgg atggcggcct gaagccacac agcgatattg atttgctggt
      841 tacggtgacc gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga
      901 aacttcggct tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt
      961 gcacgacgac atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg
     1021 gcagcgcaat gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc
     1081 tatcttgctg acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga
     1141 actctttgat ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct
     1201 atggaactcg ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg
     1261 catttggtac agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc
     1321 aatggagcgc ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct
     1381 tggacaagaa gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccatta
     1441 cgtaaaaggc gagatcacca aggtagtcgg caaataactg tcagaccaag tttactcata
     1501 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     1561 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     1621 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     1681 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     1741 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     1801 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     1861 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     1921 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     1981 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     2041 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     2101 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     2161 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     2221 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     2281 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     2341 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     2401 gagcgaggaa gcggaagagc gcccaatacg caa
//
