LOCUS       sequence-434914-        7384 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of constitutively active human IKK-2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7384)
  AUTHORS   Griffiths CD, Shah M, Shao W, Borgman CA, Janes KA
  TITLE     Three modes of viral adaption by the heart.
  JOURNAL   Sci Adv. 2024 Nov 15;10(46):eadp6303. doi: 10.1126/sciadv.adp6303. 
            Epub 2024 Nov 13.
  PUBMED    39536108
REFERENCE   2  (bases 1 to 7384)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2024-11-15"; volume: "10(46):eadp6303. doi"; pages: " 
            10.1126/sciadv.adp6303. Epub 2024 Nov 13"
COMMENT     Sequence Label: pBABE FLAG-IKK2-EE puro
FEATURES             Location/Qualifiers
     source          1..7384
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             8..477
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    542..741
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             942..1358
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1288..1310
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1326..1342
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     regulatory      1369..1378
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1378..1401
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(3714..3734)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3719..4048
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3899..4034
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4058..4657
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4058..4077)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4554..4574
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             4878..5347
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(5477..5494)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5648..6236)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5728..5747)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6396..7187,7188..7256))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7019..7038
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7257..7361)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctgcagcctg aatatgggcc aaacaggata tctgtggtaa gcagttcctg ccccggctca
       61 gggccaagaa cagatggaac agctgaatat gggccaaaca ggatatctgt ggtaagcagt
      121 tcctgccccg gctcagggcc aagaacagat ggtccccaga tgcggtccag ccctcagcag
      181 tttctagaga accatcagat gtttccaggg tgccccaagg acctgaaatg accctgtgcc
      241 ttatttgaac taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc tgctccccga
      301 gctcaataaa agagcccaca acccctcact cggggcgcca gtcctccgat tgactgagtc
      361 gcccgggtac ccgtgtatcc aataaaccct cttgcagttg catccgactt gtggtctcgc
      421 tgttccttgg gagggtctcc tctgagtgat tgactacccg tcagcggggg tctttcattt
      481 gggggctcgt ccgggatcgg gagacccctg cccagggacc accgacccac caccgggagg
      541 caagctggcc agcaacttat ctgtgtctgt ccgattgtct agtgtctatg actgatttta
      601 tgcgcctgcg tcggtactag ttagctaact agctctgtat ctggcggacc cgtggtggaa
      661 ctgacgagtt ctgaacaccc ggccgcaacc ctgggagacg tcccagggac tttgggggcc
      721 gtttttgtgg cccgacctga ggaagggagt cgatgtggaa tccgaccccg tcaggatatg
      781 tggttctggt aggagacgag aacctaaaac agttcccgcc tccgtctgaa tttttgcttt
      841 cggtttggaa ccgaagccgc gcgtcttgtc tgctgcagcg ctgcagcatc gttctgtgtt
      901 gtctctgtct gactgtgttt ctgtatttgt ctgaaaatta gggccagact gttaccactc
      961 ccttaagttt gaccttaggt cactggaaag atgtcgagcg gatcgctcac aaccagtcgg
     1021 tagatgtcaa gaagagacgt tgggttacct tctgctctgc agaatggcca acctttaacg
     1081 tcggatggcc gcgagacggc acctttaacc gagacctcat cacccaggtt aagatcaagg
     1141 tcttttcacc tggcccgcat ggacacccag accaggtccc ctacatcgtg acctgggaag
     1201 ccttggcttt tgacccccct ccctgggtca agccctttgt acaccctaag cctccgcctc
     1261 ctcttcctcc atccgccccg tctctccccc ttgaacctcc tcgttcgacc ccgcctcgat
     1321 cctcccttta tccagccctc actccttctc taggcgccgg ccggatcagc caccatggac
     1381 tacaaagacg atgacgacaa gcttgcggcc gcaatgagct ggtcaccttc cctgacaacg
     1441 cagacatgtg gggcctggga aatgaaagag cgccttggga cagggggatt tggaaatgtc
     1501 atccgatggc acaatcagga aacaggtgag cagattgcca tcaagcagtg ccggcaggag
     1561 ctcagccccc ggaaccgaga gcggtggtgc ctggagatcc agatcatgag aaggctgacc
     1621 caccccaatg tggtggctgc ccgagatgtc cctgagggga tgcagaactt ggcgcccaat
     1681 gacctgcccc tgctggccat ggagtactgc caaggaggag atctccggaa gtacctgaac
     1741 cagtttgaga actgctgtgg tctgcgggaa ggtgccatcc tcaccttgct gagtgacatt
     1801 gcctctgcgc ttagatacct tcatgaaaac agaatcatcc atcgggatct aaagccagaa
     1861 aacatcgtcc tgcagcaagg agaacagagg ttaatacaca aaattattga cctaggatat
     1921 gccaaggagc tggatcaggg cgaactttgc acagaattcg tggggaccct gcagtacctg
     1981 gccccagagc tactggagca gcagaagtac acagtgaccg tcgactactg gagcttcggc
     2041 accctggcct ttgagtgcat cacgggcttc cggcccttcc tccccaactg gcagcccgtg
     2101 cagtggcatt caaaagtgcg gcagaagagt gaggtggaca ttgttgttag cgaagacttg
     2161 aatggaacgg tgaagttttc aagctcttta ccctacccca ataatcttaa cagtgtcctg
     2221 gctgagcgac tggagaagtg gctgcaactg atgctgatgt ggcacccccg acagaggggc
     2281 acggatccca cgtatgggcc caatggctgc ttcaaggccc tggatgacat cttaaactta
     2341 aagttggttc atatcttgaa catggtcacg ggcaccatcc acacctaccc tgtgacagag
     2401 gatgagagtc tgcagagctt gaaggccaga atccaacagg acacgggcat cccagaggag
     2461 gaccaggagc tgctgcagga agcgggcctg gcgttgatcc ccgataagcc tgccactcag
     2521 tgtatttcag acggcaagtt aaatgagggc cacacattgg acatggatct tgtttttctc
     2581 tttgacaaca gtaaaatcac ctatgagact cagatctccc cacggcccca acctgaaagt
     2641 gtcagctgta tccttcaaga gcccaagagg aatctcgcct tcttccagct gaggaaggtg
     2701 tggggccagg tctggcacag catccagacc ctgaaggaag attgcaaccg gctgcagcag
     2761 ggacagcgag ccgccatgat gaatctcctc cgaaacaaca gctgcctctc caaaatgaag
     2821 aattccatgg cttccatgtc tcagcagctc aaggccaagt tggatttctt caaaaccagc
     2881 atccagattg acctggagaa gtacagcgag caaaccgagt ttgggatcac atcagataaa
     2941 ctgctgctgg cctggaggga aatggagcag gctgtggagc tctgtgggcg ggagaacgaa
     3001 gtgaaactcc tggtagaacg gatgatggct ctgcagaccg acattgtgga cttacagagg
     3061 agccccatgg gccggaagca ggggggaacg ctggacgacc tagaggagca agcaagggag
     3121 ctgtacagga gactaaggga aaaacctcga gaccagcgaa ctgagggtga cagtcaggaa
     3181 atggtacggc tgctgcttca ggcaattcag agcttcgaga agaaagtgcg agtgatctat
     3241 acgcagctca gtaaaactgt ggtttgcaag cagaaggcgc tggaactgtt gcccaaggtg
     3301 gaagaggtgg tgagcttaat gaatgaggat gagaagactg ttgtccggct gcaggagaag
     3361 cggcagaagg agctctggaa tctcctgaag attgcttgta gcaaggtccg tggtcctgtc
     3421 agtggaagcc cggatagcat gaatgcctct cgacttagcc agcctgggca gctgatgtct
     3481 cagccctcca cggcctccaa cagcttacct gagccagcca agaagagtga agaactggtg
     3541 gctgaagcac ataacctctg caccctgcta gaaaatgcca tacaggacac tgtgagggaa
     3601 caagaccaga gtttcacggc cctagactgg agctggttac agacggaaga agaagagcac
     3661 agctgcctgg agcaggcctc atgacaattc gccagcacag tggtcgaccc tgtggaatgt
     3721 gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     3781 gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag
     3841 tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat
     3901 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     3961 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
     4021 cttttttgga ggcctaggct tttgcaaaaa gcttaccatg accgagtaca agcccacggt
     4081 gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc
     4141 cgactacccc gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga
     4201 gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga
     4261 cgacggcgcc gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt
     4321 cgccgagatc ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca
     4381 gatggaaggc ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt
     4441 cggcgtctcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt
     4501 ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct
     4561 ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc
     4621 gcgcacctgg tgcatgaccc gcaagcccgg tgcctgacgc ccgccccacg acccgcagcg
     4681 cccgaccgaa aggagcgcac gaccccatgc atcgataaaa taaaagattt tatttagtct
     4741 ccagaaaaag gggggaatga aagaccccac ctgtaggttt ggcaagctag cttaagtaac
     4801 gccattttgc aaggcatgga aaaatacata actgagaata gagaagttca gatcaaggtc
     4861 aggaacagat ggaacagctg aatatgggcc aaacaggata tctgtggtaa gcagttcctg
     4921 ccccggctca gggccaagaa cagatggaac agctgaatat gggccaaaca ggatatctgt
     4981 ggtaagcagt tcctgccccg gctcagggcc aagaacagat ggtccccaga tgcggtccag
     5041 ccctcagcag tttctagaga accatcagat gtttccaggg tgccccaagg acctgaaatg
     5101 accctgtgcc ttatttgaac taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc
     5161 tgctccccga gctcaataaa agagcccaca acccctcact cggggcgcca gtcctccgat
     5221 tgactgagtc gcccgggtac ccgtgtatcc aataaaccct cttgcagttg catccgactt
     5281 gtggtctcgc tgttccttgg gagggtctcc tctgagtgat tgactacccg tcagcggggg
     5341 tctttcacat gcagcatgta tcaaaattaa tttggttttt tttcttaagt atttacatta
     5401 aatggccata gttgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     5461 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     5521 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     5581 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     5641 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     5701 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     5761 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     5821 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     5881 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     5941 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     6001 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     6061 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
     6121 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     6181 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     6241 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     6301 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttgcgg ccgcaaatca
     6361 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     6421 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     6481 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     6541 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     6601 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     6661 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     6721 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     6781 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     6841 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     6901 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     6961 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     7021 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     7081 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     7141 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     7201 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     7261 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     7321 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     7381 ccac
//
