LOCUS       sequence-43509-e         955 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 955)
  AUTHORS   Lee TS, Krupa RA, Zhang F, Hajimorad M, Holtz WJ, Prasad N, Lee SK, 
            Keasling JD
  TITLE     BglBrick vectors and datasheets: A synthetic biology platform for 
            gene expression.
  JOURNAL   J Biol Eng. 2011 Sep 20;5:12.
  PUBMED    21933410
REFERENCE   2  (bases 1 to 955)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Eng."; date: "2011-09-20"; volume: "5"; pages: "12"
COMMENT     Sequence Label: pBbA8a-RFP
FEATURES             Location/Qualifiers
     source          1..955
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      145..216
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      232..259
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     primer_bind     complement(287..304)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(501..520)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gccgggtgct tacaaaaccg acatcaaact ggacatcacc tcccacaacg aagactacac
       61 catcgttgaa cagtacgaac gtgctgaagg tcgtcactcc accggtgctt aaggatccaa
      121 actcgagtaa ggatctccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc
      181 tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct
      241 tcgggtgggc ctttctgcgt ttatacctag ggatatattc cgcttcctcg ctcactgact
      301 cgctacgctc ggtcgttcga ctgcggcgag cggaaatggc ttacgaacgg ggcggagatt
      361 tcctggaaga tgccaggaag atacttaaca gggaagtgag agggccgcgg caaagccgtt
      421 tttccatagg ctccgccccc ctgacaagca tcacgaaatc tgacgctcaa atcagtggtg
      481 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggcggct ccctcgtgcg
      541 ctctcctgtt cctgcctttc ggtttaccgg tgtcattccg ctgttatggc cgcgtttgtc
      601 tcattccacg cctgacactc agttccgggt aggcagttcg ctccaagctg gactgtatgc
      661 acgaaccccc cgttcagtcc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
      721 acccggaaag acatgcaaaa gcaccactgg cagcagccac tggtaattga tttagaggag
      781 ttagtcttga agtcatgcgc cggttaaggc taaactgaaa ggacaagttt tggtgactgc
      841 gctcctccaa gccagttacc tcggttcaaa gagttggtag ctcagagaac cttcgaaaaa
      901 ccgccctgca aggcggtttt ttcgttttca gagcaagaga ttacgcgcag accaa
//
