LOCUS       sequence-43510-e         896 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 896)
  AUTHORS   Lee TS, Krupa RA, Zhang F, Hajimorad M, Holtz WJ, Prasad N, Lee SK, 
            Keasling JD
  TITLE     BglBrick vectors and datasheets: A synthetic biology platform for 
            gene expression.
  JOURNAL   J Biol Eng. 2011 Sep 20;5:12.
  PUBMED    21933410
REFERENCE   2  (bases 1 to 896)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Eng."; date: "2011-09-20"; volume: "5"; pages: "12"
COMMENT     Sequence Label: pBbA8a-RFP
FEATURES             Location/Qualifiers
     source          1..896
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(193..477)
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     complement(251..270)
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
ORIGIN
        1 actttcagtt tagcggtctg ggtaccttcg tacggacgac cttcaccttc accttcgatt
       61 tcgaactcgt gaccgttaac ggaaccttcc atacgaactt tgaaacgcat gaactctttg
      121 ataacgtctt cgctactcgc catatgtata tctccttctt aaaagatctt ttgaattccc
      181 aaaaaaacgg gtatggagaa acagtagaga gttgcgataa aaagcgtcag gtagaatccg
      241 ctaatcttat ggataaaaat gctatggcat agcaaagtgt gacgccgtgc aaataatcaa
      301 tgtggacttt tctgccgtga ttatagacac ttttgttacg cgtttttgtc atggctttgg
      361 tcccgctttg ttacagaatg cttttaataa gcggggttac cggtttggtt agcgagaaga
      421 gccagtaaaa gacgcagtga cggcaatgtc tgatgcaata tggacaattg gtttcttctc
      481 tgaatggcgg gagtatgaaa agtatggctg aagcgcaaaa tgatcccctg ctgccgggat
      541 actcgtttaa tgcccatctg gtggcgggtt taacgccgat tgaggccaac ggttatctcg
      601 atttttttat cgaccgaccg ctgggaatga aaggttatat tctcaatctc accattcgcg
      661 gtcagggggt ggtgaaaaat cagggacgag aatttgtttg ccgaccgggt gatattttgc
      721 tgttcccgcc aggagagatt catcactacg gtcgtcatcc ggaggctcgc gaatggtatc
      781 accagtgggt ttactttcgt ccgcgcgcct actggcatga atggcttaac tggccgtcaa
      841 tatttgccaa tacggggttc tttcgcccgg atgaagcgca ccagccgcat ttcagc
//
