LOCUS       sequence-43536-e         818 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 818)
  AUTHORS   Lee TS, Krupa RA, Zhang F, Hajimorad M, Holtz WJ, Prasad N, Lee SK, 
            Keasling JD
  TITLE     BglBrick vectors and datasheets: A synthetic biology platform for 
            gene expression.
  JOURNAL   J Biol Eng. 2011 Sep 20;5:12.
  PUBMED    21933410
REFERENCE   2  (bases 1 to 818)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Eng."; date: "2011-09-20"; volume: "5"; pages: "12"
COMMENT     Sequence Label: pBbS5k-RFP
FEATURES             Location/Qualifiers
     source          1..818
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      151..222
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      238..265
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
ORIGIN
        1 tcagctgccg ggtgcttaca aaaccgacat caaactggac atcacctccc acaacgaaga
       61 ctacaccatc gttgaacagt acgaacgtgc tgaaggtcgt cactccaccg gtgcttaagg
      121 atccaaactc gagtaaggat ctccaggcat caaataaaac gaaaggctca gtcgaaagac
      181 tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc
      241 tcaccttcgg gtgggccttt ctgcgtttat acctagggta cgggttttgc tgcccgcaaa
      301 cgggctgttc tggtgttgct agtttgttat cagaatcgca gatccggctt cagccggttt
      361 gccggctgaa agcgctattt cttccagaat tgccatgatt ttttccccac gggaggcgtc
      421 actggctccc gtgttgtcgg cagctttgat tcgataagca gcatcgcctg tttcaggctg
      481 tctatgtgtg actgttgagc tgtaacaagt tgtctcaggt gttcaatttc atgttctagt
      541 tgctttgttt tactggtttc acctgttcta ttaggtgtta catgctgttc atctgttaca
      601 ttgtcgatct gttcatggtg aacagctttg aatgcaccaa aaactcgtaa aagctctgat
      661 gtatctatct tttttacacc gttttcatct gtgcatatgg acagttttcc ctttgatatg
      721 taacggtgaa cagttgttct acttttgttt gttagtcttg atgcttcact gatagataca
      781 agagccataa gaacctcaga tccttccgta tttagcca
//
