LOCUS       sequence-435775-        1716 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Co-expression of two genes in yeast with V5 and HA tags and ADE2 
            selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1716)
  AUTHORS   Dong G, Nakai T, Matsuzaki T
  TITLE     A novel plasmid-based experimental system in Saccharomyces 
            cerevisiae that enables the introduction of 10 different plasmids 
            into cells.
  JOURNAL   FEBS Open Bio. 2024 Oct 10. doi: 10.1002/2211-5463.13893.
  PUBMED    39387713
REFERENCE   2  (bases 1 to 1716)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FEBS Open 
            Bio. 2024 Oct 10. doi: 10.1002/2211-5463.13893."
COMMENT     Sequence Label: pESC-ADE (V5 and HA)
FEATURES             Location/Qualifiers
     source          1..1716
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..1716
                     /codon_start=1
                     /gene="S. cerevisiae ADE2"
                     /product="phosphoribosylaminoimidazole carboxylase, 
                     required for adenine biosynthesis"
                     /label=ADE2
                     /note="yeast auxotrophic marker"
                     /translation="MDSRTVGILGGGQLGRMIVEAANRLNIKTVILDAENSPAKQISNS
                     NDHVNGSFSNPLDIEKLAEKCDVLTIEIEHVDVPTLKNLQVKHPKLKIYPSPETIRLIQ
                     DKYIQKEHLIKNGIAVTQSVPVEQASETSLLNVGRDLGFPFVLKSRTLAYDGRGNFVVK
                     NKEMIPEALEVLKDRPLYAEKWAPFTKELAVMIVRSVNGLVFSYPIVETIHKDNICDLC
                     YAPARVPDSVQLKAKLLAENAIKSFPGCGIFGVEMFYLETGELLINEIAPRPHNSGHYT
                     IDACVTSQFEAHLRSILDLPMPKNFTSFSTITTNAIMLNVLGDKHTKDKELETCERALA
                     TPGSSVYLYGKESRPNRKVGHINIIASSMAECEQRLNYITGRTDIPIKISVAQKLDLEA
                     MVKPLVGIIMGSDSDLPVMSAACAVLKDFGVPFEVTIVSAHRTPHRMSAYAISASKRGI
                     KTIIAGAGGAAHLPGMVAAMTPLPVIGVPVKGSCLDGVDSLHSIVQMPRGVPVATVAIN
                     NSTNAALLAVRLLGAYDSSYTTKMEQFLLKQEEEVLVKAQKLETVGYEAYLENK"
ORIGIN
        1 atggattcta gaacagttgg tatattagga gggggacaat tgggacgtat gattgttgag
       61 gcagcaaaca ggctcaacat taagacggta atactagatg ctgaaaattc tcctgccaaa
      121 caaataagca actccaatga ccacgttaat ggctcctttt ccaatcctct tgatatcgaa
      181 aaactagctg aaaaatgtga tgtgctaacg attgagattg agcatgttga tgttcctaca
      241 ctaaagaatc ttcaagtaaa acatcccaaa ttaaaaattt acccttctcc agaaacaatc
      301 agattgatac aagacaaata tattcaaaaa gagcatttaa tcaaaaatgg tatagcagtt
      361 acccaaagtg ttcctgtgga acaagccagt gagacgtccc tattgaatgt tggaagagat
      421 ttgggttttc cattcgtctt gaagtcgagg actttggcat acgatggaag aggtaacttc
      481 gttgtaaaga ataaggaaat gattccggaa gctttggaag tactgaagga tcgtcctttg
      541 tacgccgaaa aatgggcacc atttactaaa gaattagcag tcatgattgt gaggtctgtt
      601 aacggtttag tgttttctta cccaattgta gagactatcc acaaggacaa tatttgtgac
      661 ttatgttatg cgcctgctag agttccggac tccgttcaac ttaaggcgaa gttgttggca
      721 gaaaatgcaa tcaaatcttt tcccggttgt ggtatatttg gtgtggaaat gttctattta
      781 gaaacagggg aattgcttat taacgaaatt gccccaaggc ctcacaactc tggacattat
      841 accattgatg cttgcgtcac ttctcaattt gaagctcatt tgagatcaat attggatttg
      901 ccaatgccaa agaatttcac atctttctcc accattacaa cgaacgccat tatgctaaat
      961 gttcttggag acaaacatac aaaagataaa gagctagaaa cttgcgaaag agcattggcg
     1021 actccaggtt cctcagtgta cttatatgga aaagagtcta gacctaacag aaaagtaggt
     1081 cacataaata ttattgcctc cagtatggcg gaatgtgaac aaaggctgaa ctacattaca
     1141 ggtagaactg atattccaat caaaatctct gtcgctcaaa agttggactt ggaagcaatg
     1201 gtcaaaccat tggttggaat catcatggga tcagactctg acttgccggt aatgtctgcc
     1261 gcatgtgcgg ttttaaaaga ttttggcgtt ccatttgaag tgacaatagt ctctgctcat
     1321 agaactccac ataggatgtc agcatatgct atttccgcaa gcaagcgtgg aattaaaaca
     1381 attatcgctg gagctggtgg ggctgctcac ttgccaggta tggtggctgc aatgacacca
     1441 cttcctgtca tcggtgtgcc cgtaaaaggt tcttgtctag atggagtaga ttctttacat
     1501 tcaattgtgc aaatgcctag aggtgttcca gtagctaccg tcgctattaa taatagtacg
     1561 aacgctgcgc tgttggctgt cagactgctt ggcgcttatg attcaagtta tacaacgaaa
     1621 atggaacagt ttttattaaa gcaagaagaa gaagttcttg tcaaagcaca aaagttagaa
     1681 actgtcggtt acgaagctta tctagaaaac aagtaa
//
