LOCUS       sequence-43610-e         699 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 699)
  AUTHORS   Lee TS, Krupa RA, Zhang F, Hajimorad M, Holtz WJ, Prasad N, Lee SK, 
            Keasling JD
  TITLE     BglBrick vectors and datasheets: A synthetic biology platform for 
            gene expression.
  JOURNAL   J Biol Eng. 2011 Sep 20;5:12.
  PUBMED    21933410
REFERENCE   2  (bases 1 to 699)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Eng."; date: "2011-09-20"; volume: "5"; pages: "12"
COMMENT     Sequence Label: pBbE7c-RFP
FEATURES             Location/Qualifiers
     source          1..699
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    195..211
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(215..234)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(216..234)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(322..341)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        547..624
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     complement(644..663)
                     /label=LacI-R
                     /note="LacI, reverse primer"
ORIGIN
        1 cacctttggt aactttcagt ttagcggtct gggtaccttc gtacggacga ccttcacctt
       61 caccttcgat ttcgaactcg tgaccgttaa cggaaccttc catacgaact ttgaaacgca
      121 tgaactcttt gataacgtct tcgctactcg ccatatgtat atctccttct taaaagatct
      181 tttgaattct gaaattgtta tccgctcaca attcccctat agtgagtcgt attaatttcg
      241 cgggatcgag atcgatctcg atcctctacg ccggacgcat cgtggccggc atcaccggcg
      301 ccacaggtgc ggttgctggc gcctatatcg ccgacatcac cgatggggaa gatcgggctc
      361 gccacttcgg gctcatgagc gcttgtttcg gcgtgggtat ggtggcaggc cccgtggccg
      421 ggggactgtt gggcgccatc tccttgcatg caccattcct tgcggcggcg gtgctcaacg
      481 gcctcaacct actactgggc tgcttcctaa tgcaggagtc gcataaggga gagcgtcgag
      541 atcccggaca ccatcgaatg gcgcaaaacc tttcgcggta tggcatgata gcgcccggaa
      601 gagagtcaat tcagggtggt gaatgtgaaa ccagtaacgt tatacgatgt cgcagagtat
      661 gccggtgtct cttatcagac cgtttcccgc gtggtgaac
//
