LOCUS       sequence-436196-        9322 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression plasmid for SARS-CoV-2 spike (1273 residues; 
            Thr1273Glu substitution).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9322)
  AUTHORS   Dey D, Qing E, He Y, Chen Y, Jennings B, Cohn W, Singh S, Gakhar L, 
            Schnicker NJ, Pierce BG, Whitelegge JP, Doray B, Orban J, Gallagher 
            T, Hasan SS
  TITLE     A single C-terminal residue controls SARS-CoV-2 spike trafficking 
            and incorporation into VLPs.
  JOURNAL   Nat Commun. 2023 Dec 15;14(1):8358. doi: 10.1038/s41467-023-44076-3.
  PUBMED    38102143
REFERENCE   2  (bases 1 to 9322)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-12-15"; volume: "14(1):8358. doi"; pages: " 
            10.1038/s41467-023-44076-3"
COMMENT     Sequence Label: SSH-pcDNA3.1-SSH-SARS-CoV-2-S-Thr1273Glu
FEATURES             Location/Qualifiers
     source          1..9322
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1956..1975)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1957..1975)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(2020..2223)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(2049..2069)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        2224..2603
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     2775..2794
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        2869..2973
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2974..3042,3043..3834)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3192..3211)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4005..4593
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4494..4513
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4747..4764
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4881..4902
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4917..4922,4923..4940,4941..4947)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4955..4971
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4960..4982
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4979..4995
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4979..4995
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    5044..5165
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5055..5074)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5109..5128
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(5341..6135)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5452..5471)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     6062..6081
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(6202..6531)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6216..6351
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6270..6289)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     6516..6536
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(6545..6973)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6655..6676)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6867..6886
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    7019..7243
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     7232..7249
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(7272..7289)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(7305..7334)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 gtacaattca cgtcctgata cagcacggcc acctggttgc ttgtattggt gccgggtgtg
       61 atcacgctca cgccgccgaa agagcagggt gtgatgtcca ggatctccag ggtctgtggg
      121 tcgcgcacgg cgtctgtggt atctgcgatg tccctgccga actgctgaaa tggcaggaac
      181 ttcttgttgg actcggtcag cacgcctgtg ccggtcaggc cgttgaagtt gaagttcacg
      241 cacttgttct tcaccagatt ggtggacttc ttggggccgc acactgttgc tggggcgtgc
      301 agcagctcaa agctcagcac caccacgcgg taaggctgat agcccacgcc gtttgtgggc
      361 tggaagccgt aggactggag tgggaaataa cagttaaagc cctccacgcc attgcaaggg
      421 gtgctgccgg cctggtagat ttctgtagag atgtccctct cgaagggctt cagattgctc
      481 tttctaaaca gccggtacag ataattgtag ttgccgccca ctttggaatc cagattgttg
      541 ctgttccagg cgatcacgca gccggtgaaa tcgtctggca gcttataatt gtagtctgcg
      601 atcttgcctg tctggccggg ggcgatctgg cgcacctcgt cgcccctgat cacgaaagaa
      661 tcggcgtaga cgttggtaaa gcacaggtca ttcagctttg tgggggacac gccatagcac
      721 ttaaaggtgc tgaaagaggc ggagttgtac agcacgctat agtcggccac gcagttgctg
      781 atccgcttcc tattccaggc gtacacgctg gcgaagcggg ttgcgttgaa cacctcgcca
      841 aatgggcaca ggtttgtgat attaggaaag cgcacgatag actcggttgg ctgcaccctg
      901 aaattggatg tctgatagat gcccttctcc acggtaaagg acttcagtgt acactttgtc
      961 tcgctcaggg ggtccagggc gcaatccact gcgtctgtga tggtgccatt ctcgttgtac
     1021 ttcagcagga aggtccgggg ctggaggtag cccacatagt aggcggcagc gccggcggtc
     1081 catccgctag aggagtcgcc gggtgtcagg tagcttctgt gcagggccag cagtgtctga
     1141 aaccgggtga tgttgatgcc gataggcaga tccaccaggg gctccagggc gctgaagccc
     1201 tgaggcaggt cgcgcaccag gttgatgggg gtgtgcttag agtagatttt gaagtagccg
     1261 tcgatattct taaacacgaa ctccctcagg ttcttgaaat tgccctgctt gccctccagg
     1321 tccatcagga aaggctggct cacgtactca aatgtgcagt tgttggcgct agaatacact
     1381 ctaaactcgg actccatcca gctcttattg ttcttgtgat agtacacgcc caggaaggga
     1441 tcattacaaa actggaactc gcacaccttg atgaccacgt tggtggcatt gttcacgatc
     1501 agcagagact gtgtcttgga gtccagtgtg gtgccaaaga tccagcctct gatgatgttg
     1561 ctcttctcgg tagaggcgaa gtacacgcca tcgttaaaag gcagcacggg attgtcgaac
     1621 cgctttgtgc cattggtgcc gctcacgtgg atggcgtgga accaggtcac gttgctaaag
     1681 aatggcagaa acagatcctg tgtagagtgc agcacggagc ttctaaacac cttgtcagga
     1741 tagtacacgc ctctggtgaa gctattagta taagcaggtg gcagctgagt ccgagttgtc
     1801 agattgacgc actgtgagga gaccagaggc agcaggacca ggaagacgaa catggtggca
     1861 tctagatgca ttcgcgaggt accgagctcg aattccacca cactggacta gtggatccga
     1921 gctcggtacc aagcttaact agccagcttg ggtctcccta tagtgagtcg tattaatttc
     1981 gataagccag taagcagtgg gttctctagt tagccagaga gctctgctta tatagacctc
     2041 ccaccgtaca cgcctaccgc ccatttgcgt caatggggcg gagttgttac gacattttgg
     2101 aaagtcccgt tgattttggt gccaaaacaa actcccattg acgtcaatgg ggtggagact
     2161 tggaaatccc cgtgagtcaa accgctatcc acgcccattg atgtactgcc aaaaccgcat
     2221 caccatggta atagcgatga ctaatacgta gatgtactgc caagtaggaa agtcccataa
     2281 ggtcatgtac tgggcataat gccaggcggg ccatttaccg tcattgacgt caataggggg
     2341 cgtacttggc atatgataca cttgatgtac tgccaagtgg gcagtttacc gtaaatactc
     2401 cacccattga cgtcaatgga aagtccctat tggcgttact atgggaacat acgtcattat
     2461 tgacgtcaat gggcgggggt cgttgggcgg tcagccaggc gggccattta ccgtaagtta
     2521 tgtaacgcgg aactccatat atgggctatg aactaatgac cccgtaattg attactatta
     2581 ataactagtc aataatcaat gtcaacgcgt atatctggcc cgtacatcgc gaagcagcgc
     2641 aaaacgccta accctaagca gattcttcat gcaattgtcg gtcaagcctt gccttgttgt
     2701 agcttaaatt ttgctcgcgc actactcagc gacctccaac acacaagcag ggagcagata
     2761 ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata ggggatcggg
     2821 agatctcccg atccgtcgac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct
     2881 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     2941 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     3001 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     3061 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     3121 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     3181 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
     3241 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     3301 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     3361 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     3421 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     3481 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
     3541 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     3601 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     3661 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     3721 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     3781 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     3841 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     3901 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     3961 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     4021 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     4081 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     4141 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     4201 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     4261 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     4321 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     4381 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     4441 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     4501 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     4561 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     4621 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     4681 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
     4741 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc
     4801 cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg
     4861 ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta
     4921 cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca
     4981 ggaaacagct atgaccatga ttacgccaag ctctagctag aggtcgacgg tatacagaca
     5041 tgataagata cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct
     5101 ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac
     5161 aagttggggt gggcgaagaa ctccagcatg agatccccgc gctggaggat catccagccg
     5221 gcgtcccgga aaacgattcc gaagcccaac ctttcataga aggcggcggt ggaatcgaaa
     5281 tctcgtgatg gcaggttggg cgtcgcttgg tcggtcattt cgcgaacccc agagtcccgc
     5341 tcagaagaac tcgtcaagaa ggcgatagaa ggcgatgcgc tgcgaatcgg gagcggcgat
     5401 accgtaaagc acgaggaagc ggtcagccca ttcgccgcca agctcttcag caatatcacg
     5461 ggtagccaac gctatgtcct gatagcggtc cgccacaccc agccggccac agtcgatgaa
     5521 tccagaaaag cggccatttt ccaccatgat attcggcaag caggcatcgc catgggtcac
     5581 gacgagatcc tcgccgtcgg gcatgcgcgc cttgagcctg gcgaacagtt cggctggcgc
     5641 gagcccctga tgctcttcgt ccagatcatc ctgatcgaca agaccggctt ccatccgagt
     5701 acgtgctcgc tcgatgcgat gtttcgcttg gtggtcgaat gggcaggtag ccggatcaag
     5761 cgtatgcagc cgccgcattg catcagccat gatggatact ttctcggcag gagcaaggtg
     5821 agatgacagg agatcctgcc ccggcacttc gcccaatagc agccagtccc ttcccgcttc
     5881 agtgacaacg tcgagcacag ctgcgcaagg aacgcccgtc gtggccagcc acgatagccg
     5941 cgctgcctcg tcctgcagtt cattcagggc accggacagg tcggtcttga caaaaagaac
     6001 cgggcgcccc tgcgctgaca gccggaacac ggcggcatca gagcagccga ttgtctgttg
     6061 tgcccagtca tagccgaata gcctctccac ccaagcggcc ggagaacctg cgtgcaatcc
     6121 atcttgttca atcatgcgaa acgatcctca tcctgtctct tgatcagatc cgaaaatgga
     6181 tatacaagct cccgggagct ttttgcaaaa gcctaggcct ccaaaaaagc ctcctcacta
     6241 cttctggaat agctcagagg cagaggcggc ctcggcctct gcataaataa aaaaaattag
     6301 tcagccatgg ggcggagaat gggcggaact gggcggagtt aggggcggga tgggcggagt
     6361 taggggcggg actatggttg ctgactaatt gagatgcatg ctttgcatac ttctgcctgc
     6421 tggggagcct ggggactttc cacacctggt tgctgactaa ttgagatgca tgctttgcat
     6481 acttctgcct gctggggagc ctggggactt tccacaccct aactgacaca cattccacag
     6541 aattaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc
     6601 ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt
     6661 tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc
     6721 tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg
     6781 tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga
     6841 aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg
     6901 ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg
     6961 ctacagggcg cgtggggata ccccctagag ccccagctgg ttctttccgc ctcagaagcc
     7021 atagagccca ccgcatcccc agcatgcctg ctattgtctt cccaatcctc ccccttgctg
     7081 tcctgcccca ccccaccccc cagaatagaa tgacacctac tcagacaatg cgatgcaatt
     7141 tcctcatttt attaggaaag gacagtggga gtggcacctt ccagggtcaa ggaaggcacg
     7201 ggggaggggc aaacaacaga tggctggcaa ctagaaggca cagtcgaggc tgatcagcgg
     7261 gtttaaactc aatggtgatg gtgatgatga ccggtatgca tattcagatc ctcttctgag
     7321 atgagttttt gttcgaaggg cccgggatcc gatttactcg taatgcagct tcacgccctt
     7381 cagcacaggt tcagagtcat cctcgtcaaa cttacagcag gagccacagc tacagcagcc
     7441 cttcaggcaa gaacagcagg atgtcataca gcacagcatg atggtcacca tcacgatggc
     7501 gatcaggccg gcgatgaagc ccagccagat gtaccagggc cacttgatgt actgctcata
     7561 cttgcccagc tcctggaggt cgatcaggct ctcgttcaga ttcttagcca cctcgttcag
     7621 gcggtcgatc tccttctgga tgttcaccac ggaggcattg atgccgctga tgtcgcccag
     7681 atccacgtca ggggaggtgt gattcttgaa atacttatcc agctcctcct taaagctgtc
     7741 cagctcgggc tggagtggat catacacggt attgttcacg atgccgatga ccacgtcaca
     7801 gttgccgctc acgaaggtgt tgtctgtggt gatgatctgg ggctcgtaga aattgcgctg
     7861 tgtcacaaac cagtgggtgc cgttggacac gaacacgccc tcccttggaa agtgggcctt
     7921 gccatcgtgg cagatggcag gggctgtggt gaagttcttc tcctgggcgg gcacgtaggt
     7981 cacgtgcaga aacaccacgc cgtgtggggc agactgaggg aaggacatca ggtgatagcc
     8041 cttgccacaa aagtccactc tcttagactg gcccagcacg cactcggaca tcttggttgc
     8101 tgccagattg gcgctggccc tgatctcggc ggccctgatc agctgctgtg tcacataggt
     8161 ctggaggctc tggagccggc cggtgatcag ccggtcgatc tgcacctctg cctccacctt
     8221 gtccagccgg ctcaggatgt cattcagcac gctagagatg gcgccgaagt tgctgctcag
     8281 ctgcttcacc agggtattca gggcctgggc gttctgattc accacatcct ggagcttgcc
     8341 cagggcgctg gctgtagagg acaggctgtc ctggatcttg ccgatggcgg agttaaactg
     8401 attggcgatc agcttctggt tctcgtacag cacattctgg gtcacgccga tgccgttgaa
     8461 cctataggcc atctgcatgg caaaggggat ctggagggcg gctcctgcgc cgaaggtcca
     8521 tccggatgtg atggtgccgg ccagcagggc gcttgtgtac tgtgcgatca tctcatctgt
     8581 cagcaggggt ggcagcacgg tcaggccatt aaacttctgg gcgcagatca ggtccctggc
     8641 tgcgatgtcg cccaggcaat cgccatactg cttgatgaag ccggcatcgg ccagggtcac
     8701 cttgttgaac agcaggtcct cgataaagct ccgcttagaa ggcttggatg gatcaggcag
     8761 gatctgggaa aaattgaagc cgccaaagtc cttgataggg ggggtcttgt agatttgctt
     8821 cacctgggcg aacacctcct gtgtgttctt atcctgctcc acggcgatgc ctgtcagggc
     8881 tctattcagc tgggtacaaa agctgccgta ctggagcagc aggttgctgc actcggtaga
     8941 atcgccacag atatacattg tgcagtccac agatgtcttg gtcatggaca cgggcaggat
     9001 ctctgtggtc acagagattg tgaagttggt tgggatggcg atgctattgt tagagtaggc
     9061 cacgctgttc tcggcgccca gggacatggt ataggcgatg atagactggg aggccacgct
     9121 ccgggctctt ctgggagagt ttgtctgggt ctggtaagag gcacagatgc cggcgccgat
     9181 tgggatgtcg cactcatagg aattgttcac gtgctctgct ccgatcaggc atccggctct
     9241 tgtctggaag acgttggagc cggtgctgta cacccgccat gttggggtca gctgatcggc
     9301 gtggatagcc acgggcacct cg
//
