LOCUS       sequence-43628-e         759 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 759)
  AUTHORS   Chandler KJ, Chandler RL, Mortlock DP
  TITLE     Identification of an ancient Bmp4 mesoderm enhancer located 46 kb 
            from the promoter.
  JOURNAL   Dev Biol. 2009 Mar 15;327(2):590-602. Epub 2009 Jan 3.
  PUBMED    19159624
REFERENCE   2  (bases 1 to 759)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Biol.";
            date: "2009-03-15"; volume: "327(2)"; pages: "590-602. Epub 2009 Jan
            3"
COMMENT     Sequence Label: pGFP-IBGFTet
FEATURES             Location/Qualifiers
     source          1..759
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     139..161
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    189..756
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(349..368)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     578..595
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
ORIGIN
        1 accaggcaaa gcgccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc
       61 gggcctcttc gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt
      121 gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacgggatct tccatggttg
      181 tggcaagctt atcatcgtgt ttttcaaagg aaaaccacgt ccccgtggtt cggggggcct
      241 agacgttttt ttaacctcga ctaaacacat gtaaagcatg tgcaccgagg ccccagatca
      301 gatcccatac aatggggtac cttctgggca tccttcagcc ccttgttgaa tacgcttgag
      361 gagagccatt tgactctttc cacaactatc caactcacaa cgtggcactg gggttgtgcc
      421 gcctttgcag gtgtatctta tacacgtggc ttttggccgc agaggcacct gtcgccaggt
      481 ggggggttcc gctgcctgca aagggtcgct acagacgttg tttgtcttca agaagcttcc
      541 agaggaactg cttccttcac gacattcaac agaccttgca ttcctttggc gagaggggaa
      601 agacccctag gaatgctcgt caagaagaca gggccaggtt tccgggccct cacattgcca
      661 aaagacggca atatggtgga aaataacata tagacaaacg cacaccggcc ttattccaag
      721 cggcttcggc cagtaacgtt aggggggggg gagggagag
//
