LOCUS       sequence-436693-        4070 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  PapE Exonuclease fused to linker 4LF2 as N-terminal tag module 
            (AATG_PapE-4*LF2_AGGT).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4070)
  AUTHORS   Schreiber T, Prange A, Schafer P, Iwen T, Grutzner R, Marillonnet S,
            Lepage A, Javelle M, Paul W, Tissier A
  TITLE     Efficient scar-free knock-ins of several kilobases in plants by 
            engineered CRISPR/Cas endonucleases.
  JOURNAL   Mol Plant. 2024 Mar 22:S1674-2052(24)00086-8. doi: 
            10.1016/j.molp.2024.03.013.
  PUBMED    38520090
REFERENCE   2  (bases 1 to 4070)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Plant. 
            2024 Mar 22:S1674-2052(24)00086-8. doi: 10.1016/j.molp.2024.03.013."
COMMENT     Sequence Label: pAGT9050
FEATURES             Location/Qualifiers
     source          1..4070
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             2302..3093
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3434..4022
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3923..3942
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aatgcagact actacccctg tggatcctcc atcttctcgg tctgaaaaca agaggcctcc
       61 tgctcctgct ggtgatgaag gtgctggtcc tggtagaggt cttgttgatc ctgctagacc
      121 tcctaagagg ccaaggcctg attctcttcc tcttgctgct gtgtgtagac ctgctactcc
      181 tccatcacct ggtagaccag aaactcctcc tactcctgat ctgcctttgt ctcctagagg
      241 aacccatggt attgcagctc cagctggcga acctgaacct ggttctccta gtctgcttga
      301 gaactatgtt cctccagctc ctgatgctgg tgacgctggt tctactccag agcctggttg
      361 gtctgctgtt gctattccag atgctctgcc ttctcatgtg ctggctgaga cttttgagag
      421 gcacctttgc ggtcttttga ggggtgttag aaggcctttg gatgttgagc ctcttagagc
      481 taggctgggc taccttttct ctcttgctac tgctcttgaa gaggctggca tggtggatag
      541 aggtgttggt ggtcatcttc tgaggctgtc tagaagggct gctgctgcag atcctagacc
      601 tcttatggct tttttcgagg ccgctactca gaaccaggct gaatctcaac tttggggtct
      661 gcttagaagg ggtcttacta ccgcttctac ccttaagtgg ggacctagag gaccttgctt
      721 tagccctaga tggctgaaga acaacggtga tcctaggctg gacttccagt catctgctgt
      781 tatgttcggt aggaccaatg agcctgctgc tagggctctt ttgttcaggt attgcgttgg
      841 cagggctgat gatagagatg ctgaaggtga tgatgctggc aggcgttttg ttttttgcga
      901 gcctggtgat gctcctgcag cttctgttca tgcttgcggt gttcttgtgg atgctcacac
      961 tggtatggtg ggcgcttctc ttgatattct tgtgtgccct agagataggc acggctgcct
     1021 taatcctgct ccaggtactc ctcttaggtt ctacgaggtt aagtgccggg ctaagtacgc
     1081 tttcgatcct gctgatgcag gcgatcctgt tgttgctgct catagaaggc ttgtggctag
     1141 gcgttctcca tctgatttca gggctttctt gcggtctatt gctaggcctg gtgtgagata
     1201 ctttgctcct ggaagagttc ctggtcctga ggaagctctt gtttctgatc atgctgtgtg
     1261 ggctgatgca agagccggtg atgagaagag aaggtgctct gctttggaca gggctcttgt
     1321 gggtcttaat tctggtgtgg cttccgatgt gctgcttttc ggagatcctg atccagagag
     1381 aaggaccgtt tctcctttgg cttggtcatc tggtgctctt gtgcacaggg aacctatctt
     1441 cgctaatcct aggcacccga acttcaagca gattctggtt caggcttacg tgctggcttc
     1501 tcattttcct gaatgccctc ttcatcctca cctcgtgact ttcattggta gacataggac
     1561 ccctgatgag gaaggacttt ctctgagact tgaggacgct ccagcttctg ctccagcagc
     1621 tgttagggct gcagctggtg cttctattct tccagatcag gctgttcctg tggctctgat
     1681 tattacccct gttagggttg acgctgccat ctacgatctt atccgtagga actctaggct
     1741 cgccttcgat gagactcttg ctagactttg ggcttctagg gctcctgctt ctgatcctgc
     1801 cgctgctggc gaaacttctt cgggtggagg tggttctggt ggaggtggat caggaggagg
     1861 aggttcgggt ggaggtggtt ctggtggagg tggatcagga ggaggaggat caggtggagg
     1921 tggttctggt ggaggtggat caggaggagg aggttcaggt ggaggtggtt ctggtggagg
     1981 tggatcagga ggaggaggat caggttgaga ccacgaagtg ccgaggcacg aacccagtgg
     2041 acataagcct gttcggttcg taagctgtaa tgcaagtagc gtatgcgctc acgcaactgg
     2101 tccagaacct tgaccgaacg cagcggtggt aacggcgcag tggcggtttt catggcttgt
     2161 tatgactgtt tttttggggt acagtctatg cctcgggcat ccaagcagca agcgcgttac
     2221 gccgtgggtc gatgtttgat gttatggagc agcaacgatg ttacgcagca gggcagtcgc
     2281 cctaaaacaa agttaaacat catgggggaa gcggtgatcg ccgaagtatc gactcaacta
     2341 tcagaggtag ttggcgtcat cgagcgccat ctcgaaccga cgttgctggc cgtacatttg
     2401 tacggctccg cagtggatgg cggcctgaag ccacacagcg atattgattt gctggttacg
     2461 gtgaccgtaa ggcttgatga aacaacgcgg cgagctttga tcaacgacct tttggaaact
     2521 tcggcttccc ctggagagag cgagattctc cgcgctgtag aagtcaccat tgttgtgcac
     2581 gacgacatca ttccgtggcg ttatccagct aagcgcgaac tgcaatttgg agaatggcag
     2641 cgcaatgaca ttcttgcagg tatcttcgag ccagccacga tcgacattga tctggctatc
     2701 ttgctgacaa aagcaagaga acatagcgtt gccttggtag gtccagcggc ggaggaactc
     2761 tttgatccgg ttcctgaaca ggatctattt gaggcgctaa atgaaacctt aacgctatgg
     2821 aactcgccgc ccgactgggc tggcgatgag cgaaatgtag tgcttacgtt gtcccgcatt
     2881 tggtacagcg cagtaaccgg caaaatcgcg ccgaaggatg tcgctgccga ctgggcaatg
     2941 gagcgcctgc cggcccagta tcagcccgtc atacttgaag ctagacaggc ttatcttgga
     3001 caagaagaag atcgcttggc ctcgcgcgca gatcagttgg aagaatttgt ccattacgtg
     3061 aaaggcgaga tcaccaaggt agtcggcaaa taatgtctag ctagaaattc gttcaagccg
     3121 acgccgcttc gcggcgcggc ttaactcaag cgttagatgc actaagcaca taattgctca
     3181 cagccaaact atcaggtcaa gtctgctttt attattttta agcgtgcata ataagcccta
     3241 cacaaattgg gagatatatc atgctgtcag accaagttta ctcatatata ctttagattg
     3301 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     3361 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     3421 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     3481 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     3541 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     3601 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     3661 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     3721 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     3781 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     3841 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     3901 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     3961 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     4021 aaaacgccag caacgcggcc tttttacggt tcctgcactc tgtggtctca
//
