LOCUS       sequence-43690-e         843 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 843)
  AUTHORS   Lee TS, Krupa RA, Zhang F, Hajimorad M, Holtz WJ, Prasad N, Lee SK, 
            Keasling JD
  TITLE     BglBrick vectors and datasheets: A synthetic biology platform for 
            gene expression.
  JOURNAL   J Biol Eng. 2011 Sep 20;5:12.
  PUBMED    21933410
REFERENCE   2  (bases 1 to 843)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Eng."; date: "2011-09-20"; volume: "5"; pages: "12"
COMMENT     Sequence Label: pBbB2c-GFP
FEATURES             Location/Qualifiers
     source          1..843
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        40..95
                     /gene="tetR"
                     /label=tetR/tetA promoters
                     /note="overlapping promoters for bacterial tetR and tetA"
     protein_bind    41..59
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     CDS             111..737
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MMSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGT
                     RPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPT
                     TDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(187..206)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
ORIGIN
        1 atatgtatat ctccttctta aaagatcttt tgaattcttt tctctatcac tgatagggag
       61 tggtaaaata actctatcaa cgatagagtg tcaacaaaaa ttaggaatta atgatgtcta
      121 gattagataa aagtaaagtg attaacagcg cattagagct gcttaatgag gtcggaatcg
      181 aaggtttaac aacccgtaaa ctcgcccaga agctaggtgt agagcagcct acattgtatt
      241 ggcatgtaaa aaataagcgg gctttgctcg acgccttagc cattgagatg ttagataggc
      301 accatactca cttttgccct ttagaagggg aaagctggca agatttttta cgtaataacg
      361 ctaaaagttt tagatgtgct ttactaagtc atcgcgatgg agcaaaagta catttaggta
      421 cacggcctac agaaaaacag tatgaaactc tcgaaaatca attagccttt ttatgccaac
      481 aaggtttttc actagagaat gcattatatg cactcagcgc tgtggggcat tttactttag
      541 gttgcgtatt ggaagatcaa gagcatcaag tcgctaaaga agaaagggaa acacctacta
      601 ctgatagtat gccgccatta ttacgacaag ctatcgaatt atttgatcac caaggtgcag
      661 agccagcctt cttattcggc cttgaattga tcatatgcgg attagaaaaa caacttaaat
      721 gtgaaagtgg gtcttaagac gtcgatatct ggcgaaaatg agacgttgat cggcacgtaa
      781 gaggttccaa ctttcaccat aatgaaataa gatcactacc gggcgtattt tttgagttat
      841 cga
//
