LOCUS       sequence-436982-       12308 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector expressing luciferase and mCherry..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12308)
  AUTHORS   Kimbrel EA, Davis TN, Bradner JE, Kung AL
  TITLE     In vivo pharmacodynamic imaging of proteasome inhibition.
  JOURNAL   Mol Imaging. 2009 May-Jun;8(3):140-7.
  PUBMED    19723471
REFERENCE   2  (bases 1 to 12308)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol 
            Imaging. 2009 May-Jun"; volume: "8(3)"; pages: "140-7"
COMMENT     Sequence Label: FUW-Luc-mCherry-puro
FEATURES             Location/Qualifiers
     source          1..12308
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     772..792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2622..3833
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     3670..3689
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     regulatory      3868..3877
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5533..5592
                     /codon_start=1
                     /product="2A peptide from equine rhinitis A virus 
                     polyprotein"
                     /label=E2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="QCTNYALLKLAGDVESNPGP"
     primer_bind     complement(5744..5762)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     CDS             6322..6375
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     misc_feature    7000..7588
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7053..7073)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7471..7482)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7591..7607)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7592..7608)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             8113..8293
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8319..8336)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8325..8549
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8595..9023
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8682..8701)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8892..8913
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9032..9052)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9037..9366
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9217..9352
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9279..9298
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        9414..9461
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9480..9854
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    9984..10105
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10021..10040)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10075..10094
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10154..10170)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10154..10170)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10167..10189)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10178..10194
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10202..10208,10209..10226,10227..10232))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10247..10268
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10385..10402)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10556..11144)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10636..10655)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11315..12106,12107..12175))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11938..11957
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12176..12280)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatta acccgtgtcg gctccagatc tggcctccgc gccgggtttt
     2641 ggcgcctccc gcgggcgccc ccctcctcac ggcgagcgct gccacgtcag acgaagggcg
     2701 cagcgagcgt cctgatcctt ccgcccggac gctcaggaca gcggcccgct gctcataaga
     2761 ctcggcctta gaaccccagt atcagcagaa ggacatttta ggacgggact tgggtgactc
     2821 tagggcactg gttttctttc cagagagcgg aacaggcgag gaaaagtagt cccttctcgg
     2881 cgattctgcg gagggatctc cgtggggcgg tgaacgccga tgattatata aggacgcgcc
     2941 gggtgtggca cagctagttc cgtcgcagcc gggatttggg tcgcggttct tgtttgtgga
     3001 tcgctgtgat cgtcacttgg tgagtagcgg gctgctgggc tggccggggc tttcgtggcc
     3061 gccgggccgc tcggtgggac ggaagcgtgt ggagagaccg ccaagggctg tagtctgggt
     3121 ccgcgagcaa ggttgccctg aactgggggt tggggggagc gcagcaaaat ggcggctgtt
     3181 cccgagtctt gaatggaaga cgcttgtgag gcgggctgtg aggtcgttga aacaaggtgg
     3241 ggggcatggt gggcggcaag aacccaaggt cttgaggcct tcgctaatgc gggaaagctc
     3301 ttattcgggt gagatgggct ggggcaccat ctggggaccc tgacgtgaag tttgtcactg
     3361 actggagaac tcggtttgtc gtctgttgcg ggggcggcag ttatggcggt gccgttgggc
     3421 agtgcacccg tacctttggg agcgcgcgcc ctcgtcgtgt cgtgacgtca cccgttctgt
     3481 tggcttataa tgcagggtgg ggccacctgc cggtaggtgt gcggtaggct tttctccgtc
     3541 gcaggacgca gggttcgggc ctagggtagg ctctcctgaa tcgacaggcg ccggacctct
     3601 ggtgagggga gggataagtg aggcgtcagt ttctttggtc ggttttatgt acctatcttc
     3661 ttaagtagct gaagctccgg ttttgaacta tgcgctcggg gttggcgagt gtgttttgtg
     3721 aagtttttta ggcacctttt gaaatgtaat catttgggtc aatatgtaat tttcagtgtt
     3781 agactagtaa attgtccgct aaattctggc cgtttttggc ttttttgtta gacgaagctt
     3841 gggctgcagg tcgactctag aggatccgcc accatggaag atgccaagaa catcaagaaa
     3901 ggccctgccc ccttctaccc cctggaagat ggcacagccg gcgagcagct gcacaaggcc
     3961 atgaagagat acgccctcgt ccctggaaca atcgccttca ccgacgccca catcgaggtg
     4021 gacatcacct acgccgagta cttcgagatg agcgtgcggc tggccgaggc catgaaacgc
     4081 tacggcctga acaccaacca ccggatcgtg gtgtgcagcg agaacagcct gcagttcttc
     4141 atgcccgtgc tgggcgccct gttcatcggc gtggccgtgg cccctgccaa cgacatctac
     4201 aacgagcggg agctgctgaa cagcatgggc atcagccagc ccaccgtggt gttcgtgagc
     4261 aagaagggcc tgcagaaaat cctgaacgtg cagaagaagc tgcccatcat ccagaaaatc
     4321 atcatcatgg acagcaagac cgactaccag ggcttccaga gcatgtacac cttcgtgacc
     4381 agccacctgc cccctggctt caacgagtac gacttcgtgc ccgagagctt cgacagggac
     4441 aagaccatcg ccctgatcat gaacagcagc ggcagcaccg gcctgcctaa aggcgtggcc
     4501 ctgcctcacc ggaccgcctg cgtgcggttc agccacgccc gggaccccat cttcggcaac
     4561 cagatcatcc ccgacaccgc catcctgagc gtggtgccct tccaccacgg cttcggcatg
     4621 ttcaccaccc tgggctacct gatctgcggc ttccgggtgg tgctgatgta ccggttcgag
     4681 gaagagctgt tcctgcggag cctgcaggac tacaagatcc agagcgccct gctggtgccc
     4741 accctgttca gctttttcgc caagagcacc ctgatcgaca agtacgacct gagcaacctg
     4801 cacgagatcg ccagcggcgg agcccccctg agcaaagaag tgggcgaagc cgtcgccaag
     4861 cggttccacc tgcccggcat ccggcagggc tatggcctga ccgagaccac aagcgccatt
     4921 ctgatcaccc ccgagggcga cgacaagcct ggcgccgtgg gcaaggtggt gcctttcttc
     4981 gaggccaagg tggtggacct ggacaccggc aagaccctgg gcgtgaacca gcggggcgag
     5041 ctgtgcgtga ggggccccat gatcatgagc ggctacgtga acaaccccga ggccaccaac
     5101 gccctgattg acaaggacgg ctggctgcac agcggcgaca tcgcctactg ggacgaggac
     5161 gagcacttct tcatcgtgga ccggctgaag agcctgatca agtacaaggg ctaccaggtg
     5221 gccccagccg agctggaaag catcctgctg cagcacccca acatcttcga cgccggggtg
     5281 gccggcctgc ccgacgatga cgccggcgag ctgcctgccg ccgtggtggt cctcgaacat
     5341 ggcaaaacca tgaccgagaa agaaatcgtg gactacgtgg ccagccaggt gaccaccgcc
     5401 aagaagctga gaggcggcgt cgtgtttgtg gacgaggtgc ccaagggcct gaccggcaag
     5461 ctggacgccc ggaagatccg ggagatcctg attaaggcca agaagggcgg caagatcgcc
     5521 gtgggctccg gccagtgcac caactacgcc ctgctgaagc tggccggcga cgtggagagc
     5581 aaccctggcc ctttcgaaat ggtgtccaag ggcgaagagg acaacatggc catcatcaaa
     5641 gagtttatgc ggttcaaggt gcacatggaa ggcagcgtga acggccacga gttcgagatc
     5701 gagggcgagg gcgaaggcag gccctacgag ggcacccaga ccgccaagct gaaggtgacc
     5761 aagggcggac ctctgccctt cgcctgggac atcctgagcc cccagttcat gtacggcagc
     5821 aaggcctacg tgaagcaccc cgccgacatc cccgactacc tgaagctgtc cttccccgag
     5881 ggcttcaagt gggagcgggt gatgaacttc gaggacggcg gggtggtgac cgtgacccag
     5941 gatagctccc tgcaggacgg cgagttcatc tacaaggtga aactgcgggg caccaacttc
     6001 cccagcgacg gccccgtgat gcagaaaaag acaatgggct gggaggccag cagcgagcgg
     6061 atgtaccccg aggatggcgc cctgaagggc gagatcaagc agcggctgaa gctgaaggac
     6121 ggcggccact acgacgccga ggtgaaaacc acctacaagg ccaaaaagcc cgtgcagctg
     6181 cctggcgcct acaacgtgaa catcaagctg gatatcacca gccacaacga ggactacacc
     6241 atcgtggagc agtacgagcg ggccgagggc agacacagca ccggcggcat ggacgagctg
     6301 tacaagctca gcggcagcgg cgagggcaga ggcagcctgc tgacctgcgg cgatgtggag
     6361 gaaaacccag gcccaatgac cgagtacaag cctaccgtga ggctggccac ccgggacgac
     6421 gtgcccagag ccgtgaggac actggccgcc gccttcgccg actaccccgc cacccggcac
     6481 accgtggacc ccgaccggca catcgagcgg gtgaccgagc tgcaggaact gttcctgacc
     6541 agagtgggcc tggatatcgg caaagtgtgg gtggccgacg acggagccgc cgtggccgtg
     6601 tggaccaccc ccgagtccgt ggaggccgga gccgtgtttg ccgagatcgg ccccaggatg
     6661 gccgagctgt ccggcagcag gctggccgcc cagcagcaga tggaaggcct gctggcccct
     6721 caccggccca aagagcccgc ctggttcctg gccaccgtgg gcgtgagccc cgaccaccag
     6781 ggcaagggcc tgggcagcgc cgtggtgctg ccaggcgtgg aagccgccga gagggccgga
     6841 gtgcccgcct tcctggaaac cagcgccccc aggaacctgc ccttctacga gcggctgggc
     6901 tttaccgtga ccgccgacgt ggaggtgcca gagggcccca ggacctggtg catgacccgg
     6961 aagccaggcg cctgagaatt cgatatcaag cttatcgata atcaacctct ggattacaaa
     7021 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac
     7081 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc
     7141 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt
     7201 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc
     7261 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc
     7321 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg
     7381 gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt
     7441 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc
     7501 cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt
     7561 cggatctccc tttgggccgc ctccccgcat cgataccgtc gacctcgaga cctagaaaaa
     7621 catggagcaa tcacaagtag caatacagca gctaccaatg ctgattgtgc ctggctagaa
     7681 gcacaagagg aggaggaggt gggttttcca gtcacacctc aggtaccttt aagaccaatg
     7741 acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg
     7801 ctaattcact cccaacgaag acaagatatc cttgatctgt ggatctacca cacacaaggc
     7861 tacttccctg attggcagaa ctacacacca gggccaggga tcagatatcc actgaccttt
     7921 ggatggtgct acaagctagt accagttgag caagagaagg tagaagaagc caatgaagga
     7981 gagaacaccc gcttgttaca ccctgtgagc ctgcatggga tggatgaccc ggagagagaa
     8041 gtattagagt ggaggtttga cagccgccta gcatttcatc acatggcccg agagctgcat
     8101 ccggactgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
     8161 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
     8221 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
     8281 aaaatctcta gcagggcccg tttaaacccg ctgatcagcc tcgactgtgc cttctagttg
     8341 ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc
     8401 cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc
     8461 tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag
     8521 gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc
     8581 tagggggtat ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
     8641 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc
     8701 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt
     8761 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg
     8821 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac
     8881 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta
     8941 ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat
     9001 ttaacaaaaa tttaacgcga attaattctg tggaatgtgt gtcagttagg gtgtggaaag
     9061 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     9121 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     9181 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
     9241 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
     9301 gcctctgcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
     9361 tgcaaaaagc tcccgggagc ttgtatatcc attttcggat ctgatcagca cgtgttgaca
     9421 attaatcatc ggcatagtat atcggcatag tataatacga caaggtgagg aactaaacca
     9481 tggccaagtt gaccagtgcc gttccggtgc tcaccgcgcg cgacgtcgcc ggagcggtcg
     9541 agttctggac cgaccggctc gggttctccc gggacttcgt ggaggacgac ttcgccggtg
     9601 tggtccggga cgacgtgacc ctgttcatca gcgcggtcca ggaccaggtg gtgccggaca
     9661 acaccctggc ctgggtgtgg gtgcgcggcc tggacgagct gtacgccgag tggtcggagg
     9721 tcgtgtccac gaacttccgg gacgcctccg ggccggccat gaccgagatc ggcgagcagc
     9781 cgtgggggcg ggagttcgcc ctgcgcgacc cggccggcaa ctgcgtgcac ttcgtggccg
     9841 aggagcagga ctgacacgtg ctacgagatt tcgattccac cgccgccttc tatgaaaggt
     9901 tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca
     9961 tgctggagtt cttcgcccac cccaacttgt ttattgcagc ttataatggt tacaaataaa
    10021 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
    10081 tgtccaaact catcaatgta tcttatcatg tctgtatacc gtcgacctct agctagagct
    10141 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
    10201 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
    10261 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
    10321 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
    10381 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
    10441 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
    10501 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
    10561 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
    10621 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
    10681 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
    10741 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
    10801 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
    10861 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
    10921 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
    10981 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
    11041 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
    11101 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
    11161 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
    11221 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
    11281 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
    11341 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
    11401 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
    11461 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
    11521 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
    11581 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
    11641 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
    11701 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
    11761 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
    11821 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
    11881 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
    11941 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
    12001 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
    12061 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
    12121 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
    12181 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
    12241 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
    12301 cacctgac
//
