LOCUS       sequence-437050-       10428 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of full-length human DCAF1. N-terminal NanoLuc 
            luciferase tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10428)
  AUTHORS   Ackloo S, Li F, Szewczyk M, Seitova A, Loppnau P, Zeng H, Xu J, 
            Ahmad S, Arnautova YA, Baghaie AJ, Beldar S, Bolotokova A, Centrella
            PA, Chau I, Clark MA, Cuozzo JW, Dehghani-Tafti S, Disch JS, Dong A,
            Dumas A, Feng JA, Ghiabi P, Gibson E, Gilmer J, Goldman B, Green SR,
            Guie MA, Guilinger JP, Harms N, Herasymenko O, Houliston S, 
            Hutchinson A, Kearnes S, Keefe AD, Kimani SW, Kramer T, Kutera M, 
            Kwak HA, Lento C, Li Y, Liu J, Loup J, Machado RAC, Mulhern CJ, 
            Perveen S, Righetto GL, Riley P, Shrestha S, Sigel EA, Silva M, 
            Sintchak MD, Slakman BL, Taylor RD, Thompson J, Torng W, 
            Underkoffler C, von Rechenberg M, Walsh RT, Watson I, Wilson DJ, 
            Wolf E, Yadav M, Yazdi AK, Zhang J, Zhang Y, Santhakumar V, Edwards 
            AM, Barsyte-Lovejoy D, Schapira M, Brown PJ, Halabelian L, 
            Arrowsmith CH
  TITLE     A Target Class Ligandability Evaluation of WD40 Repeat-Containing 
            Proteins.
  JOURNAL   J Med Chem. 2025 Jan 23;68(2):1092-1112. doi: 
            10.1021/acs.jmedchem.4c02010. Epub 2024 Nov 4.
  PUBMED    39495097
REFERENCE   2  (bases 1 to 10428)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acs.jmedchem.4c02010"; journalName: "J Med Chem"; date: 
            "2025-01-23- 23"; volume: "68"; issue: "2"; pages: "1092-1112"
COMMENT     Sequence Label: pNLF1-N_DCAF1:1-1507
FEATURES             Location/Qualifiers
     source          1..10428
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(1305..1316)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4682..4700)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     polyA_signal    4732..4853
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4743..4762)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4797..4816
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        5098..5455
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        5098..5289
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(5121..5141)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      5306..5441
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5486..6523
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     EVGRV"
     RBS             6502..6510
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     polyA_signal    6547..6595
                     /note="synthetic polyadenylation signal"
     promoter        6751..6855
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6856..6924,6925..7716)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7074..7093)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      complement(7874..8462)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7954..7973)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    8578..8861
                     /label=cer region
                     /note="ColE1-derived recombination site that helps to 
                     maintain plasmids as monomers"
     enhancer        9052..9431
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        9432..9635
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     9586..9606
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     intron          9771..9903
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     9886..9905
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     primer_bind     9947..9966
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        9947..9965
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(9979..10428,1..63)
                     /codon_start=1
                     /product="NanoLuc(R) luciferase"
                     /label=Nluc
                     /note="human codon-optimized"
                     /translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQR
                     IVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGV
                     TPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGW
                     RLCERILA"
ORIGIN
        1 ctgctgttcc gagtaaccat caacggagtg accggctggc ggctgtgcga acgcattctg
       61 gcgggctcga gcggcgcgat cgcttccgaa ttcatgacta cagtagtggt acatgtggac
      121 tccaaagctg agctcactac cctgctggag cagtgggaaa aggaacatgg cagtgggcag
      181 gacatggtac ctatccttac caggatgtct caattgattg aaaaagaaac tgaagagtat
      241 cgtaaagggg atccagaccc atttgatgat cgacatcctg gtcgagctga tccagagtgt
      301 atgctgggcc acttgctgag aatactcttc aagaatgatg atttcatgaa tgcactggtg
      361 aatgcatatg tgatgacaag ccgagagccc cctttaaaca ctgcagcttg cagactccta
      421 ttagacatca tgccagggct ggaaactgct gtcgtctttc aagaaaagga gggaattgtc
      481 gagaatcttt tcaaatgggc ccgagaggcc gatcaaccat tgaggacata ttctactgga
      541 ctgttaggag gtgctatgga aaatcaagac attgctgcca actatagaga tgaaaattca
      601 cagctggtgg caatagtgct tcgaagactg agggagctac agctacagga agtggctttg
      661 cggcaggaaa acaagcgtcc cagtccacgg aagctctctt ctgaacccct tttgcctctg
      721 gatgaggagg ctgtggatat ggactatggt gacatggctg tagatgtagt ggatggagac
      781 caagaggaag cttctggaga catggagatc tcctttcatc ttgattcagg ccacaagact
      841 agtagcagag tgaactcaac aaccaaacct gaggatggag gattaaagaa aaacaagtca
      901 gcaaaacagg gtgacagaga gaactttagg aaagccaagc aaaagttggg tttctcatct
      961 tctgatccag atcgcatgtt tgttgagctg tctaatagca gttggtcaga aatgtctccc
     1021 tgggtgattg gcaccaatta taccctttat cctatgactc ctgctatcga gcagcgactc
     1081 attctccaat atttgacccc tctaggagaa tatcaggagc tacttcccat attcatgcaa
     1141 cttggatcac gggagctgat gatgttctat attgacctga agcaaactaa tgatgtcctg
     1201 cttacatttg aggcactaaa gcacctagca tctctcctgc tccataacaa atttgccaca
     1261 gaatttgttg cgcatggtgg agtacagaaa ttactggaaa tacctcgtcc ttctatggct
     1321 gcaaccggtg tgtctatgtg tttgtattac ctatcctaca atcaggatgc catggaaaga
     1381 gtttgcatgc atccccacaa tgttctgtct gatgtggtga actataccct gtggttaatg
     1441 gagtgttctc atgcttcagg atgctgccat gctaccatgt ttttttcaat ttgcttctca
     1501 tttcgggccg tcttggagct ctttgaccgc tatgatggtc ttcgtcgtct ggtgaacttg
     1561 atcagtactt tggagattct aaatttggaa gatcagggtg cacttctgag tgatgatgaa
     1621 atatttgcta gccgccaaac tgggaaacat acctgcatgg ccttgcgcaa atactttgag
     1681 gctcacctgg ccattaaatt ggaacaagtg aagcagtcac ttcagaggac tgagggtggc
     1741 attcttgtcc acccacaacc cccgtacaag gcatgctcat atactcatga acagattgtg
     1801 gaaatgatgg aatttttgat agaatatggc ccagcgcagc tatattggga accagctgaa
     1861 gttttcctca aactttcttg tgtgcaactc ttgttgcagc ttatttctat tgcctgcaat
     1921 tggaagacct attatgcaag gaatgacact gtgcgctttg ctttggatgt cctggctatt
     1981 cttactgtgg tgccaaaaat ccagctccag ttggcagaat cagtggacgt gttggatgag
     2041 gctggatcta cagtctctac tgtaggtatc agcattattt tgggagtggc tgagggtgag
     2101 ttcttcatcc atgatgctga aattcagaag tcagcacttc agattatcat caattgtgtg
     2161 tgtggcccag ataaccgaat atccagtatt ggtaaattta tctctggtac tcctcggaga
     2221 aagctgcctc agaaccctaa aagcagtgag cacaccctgg ccaagatgtg gaatgtggtt
     2281 cagtccaaca acggcatcaa ggtgctcctg tccttactgt ccattaagat gcccatcaca
     2341 gatgcagacc aaatccgggc cctggcctgc aaagccctag tgggcctgtc tcgcagtagc
     2401 actgtccggc agatcatcag taaactgccc cttttcagca gctgccagat ccagcagctg
     2461 atgaaggagc ctgtgctgca ggacaagcgc agtgaccatg tcaagttctg caagtatgct
     2521 gctgaactca ttgaacgggt gtcaggaaaa ccacttctca ttggcactga tgtttcccta
     2581 gcacgactgc agaaagcaga tgttgttgcc cagtcaagga tctccttccc tgagaaagag
     2641 ctgcttttgt tgatacgaaa ccatcttatt tctaaagggc ttggagaaac agcaaccgtg
     2701 ctgacaaaag aggctgacct gcccatgact gctgcctccc attcttctgc ctttacccca
     2761 gtcactgctg ctgcttctcc tgtctctcta ccccgaaccc ctcgtatcgc taatggcatt
     2821 gcaactcgtc tgggcagcca tgctgctgtg ggtgcctctg cgccttctgc ccctactgct
     2881 catcctcagc cacggccccc ccagggtccg ctagctctgc ccggcccatc ttatgcaggc
     2941 aactcccctt tgattggtag aatcagtttt atcagagaga ggccatcacc ctgcaatggc
     3001 aggaaaatca gagtgttgcg gcagaagtcg gaccatggtg cctacagcca aagcccagcc
     3061 ataaaaaaac agctggacag acatcttcct tccccaccta cgctggacag tataatcaca
     3121 gagtatctta gagaacaaca tgctcgctgc aagaatccag ttgccacctg cccacctttc
     3181 tccctcttta ctcctcacca atgtcctgag ccaaaacaga ggcggcaagc gccaataaac
     3241 tttacgtcaa ggctaaaccg cagggcatca tttccaaagt atggaggggt ggatggcgga
     3301 tgctttgata ggcaccttat ctttagcaga ttccgtccta tttcagtgtt ccgggaagcc
     3361 aatgaagatg agagtggctt cacctgctgt gcattctcag cacgggagcg gttcctgatg
     3421 cttggcacct gcacagggca gctgaagctc tataatgtgt ttagtggaca ggaggaggcc
     3481 agctataact gtcacaactc agccatcaca catcttgaac cttccaggga tgggtccttg
     3541 ctgctgacat ctgctacttg gagccagcct ttgtctgcac tttggggaat gaagtcagta
     3601 tttgatatga agcattcctt cacagaagat cactatgttg agttcagtaa gcactcccag
     3661 gatcgggtca tcggcacaaa aggagacatt gcccacattt atgatattca gactggcaac
     3721 aagctgttga ctctgtttaa cccagatctt gccaacaact acaagaggaa ctgtgccacc
     3781 tttaatccta cagatgatct tgtcttaaat gatggcgtcc tctgggatgt ccgctctgca
     3841 caggccatcc acaagtttga caagttcaat atgaacatca gtggtgtttt ccatccaaat
     3901 ggactggagg tgatcattaa tactgagatt tgggaccttc gaacttttca tcttttgcat
     3961 actgttcccg ctctggatca gtgtcgcgtg gtgttcaatc acacgggaac agtgatgtat
     4021 ggagctatgt tgcaggcaga tgatgaagat gacttaatgg aagagaggat gaaaagcccc
     4081 tttgggtcat ccttccgaac atttaatgca actgactaca aacctatagc aaccattgat
     4141 gtgaaacgga acatctttga cctgtgtaca gacaccaaag actgctatct tgctgtcatt
     4201 gagaatcaag gcagcatgga tgccctgaac atggacacag tatgcaggct gtatgaagtg
     4261 ggcaggcagc gtctggcaga ggatgaggat gaagaggagg accaggaaga ggaagaacag
     4321 gaggaagaag atgatgatga agatgatgat gacaccgatg atttagatga gcttgacact
     4381 gaccagttgc tggaggcgga gttggaggag gacgacaata atgagaacgc aggggaagat
     4441 ggggacaatg acttctctcc ctctgatgag gagctagcaa accttctaga ggagggagag
     4501 gacggggagg atgaagactc tgatgcagat gaggaggtgg aactgatcct gggggacact
     4561 gacagctctg acaactctga tttggaagat gacatcatct tatctctgaa tgagtaataa
     4621 tctagatttg ggcccaattc ctgcaggatt ttgcggccgc ttgctgagtt ggctgctgcc
     4681 accgctgagc aataactagc ataacccctt ggccgcttcg agcagacatg ataagataca
     4741 ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa
     4801 tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca
     4861 acaattgcat tcattttatg tttcaggttc agggggagat gtgggaggtt tttttaagca
     4921 agtaaaacct ctacaaatgt ggtaaaatcg aattttaaca aaatattaac gcttacaatt
     4981 tcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atacgcggat
     5041 ctgcgcagca ccatggcctg aaataacctc tgaaagagga acttggttag gtaccttctg
     5101 aggcggaaag aaccagctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc
     5161 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa
     5221 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     5281 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
     5341 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc
     5401 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct
     5461 cgattcttct gacactagcg ccaccatgaa gaagcccgaa ctcaccgcta ccagcgttga
     5521 aaaatttctc atcgagaagt tcgacagtgt gagcgacctg atgcagttgt cggagggcga
     5581 agagagccga gccttcagct tcgatgtcgg cggacgcggc tatgtactgc gggtgaatag
     5641 ctgcgctgat ggcttctaca aagaccgcta cgtgtaccgc cacttcgcca gcgctgcact
     5701 acccatcccc gaagtgttgg acatcggcga gttcagcgag agcctgacat actgcatcag
     5761 tagacgcgcc caaggcgtta ctctccaaga cctccccgaa acagagctgc ctgctgtgtt
     5821 acagcctgtc gccgaagcta tggatgctat tgccgccgcc gacctcagtc aaaccagcgg
     5881 cttcggccca ttcgggcccc aaggcatcgg ccagtacaca acctggcggg atttcatttg
     5941 cgccattgct gatccccatg tctaccactg gcagaccgtg atggacgaca ccgtgtccgc
     6001 cagcgtagct caagccctgg acgaactgat gctgtgggcc gaagactgtc ccgaggtgcg
     6061 ccacctcgtc catgccgact tcggcagcaa caacgtcctg accgacaacg gccgcatcac
     6121 cgccgtaatc gactggtccg aagctatgtt cggggacagt cagtacgagg tggccaacat
     6181 cttcttctgg cggccctggc tggcttgcat ggagcagcag actcgctact tcgagcgccg
     6241 gcatcccgag ctggccggca gccctcgtct gcgagcctac atgctgcgca tcggcctgga
     6301 tcagctctac cagagcctcg tggacggcaa cttcgacgat gctgcctggg ctcaaggccg
     6361 ctgcgatgcc atcgtccgca gcggggccgg caccgtcggt cgcacacaaa tcgctcgccg
     6421 gagcgcagcc gtatggaccg acggctgcgt cgaggtgctg gccgacagcg gcaaccgccg
     6481 gcccagtaca cgaccgcgcg ctaaggaggt aggtcgagtt tagactctag ccatcacgat
     6541 ggccgcaata aaatatcttt attttcatta catctgtgtg ttggtttttt gtgtgaatcg
     6601 atagcgataa ggatcctctt tgcgcttgcg ttttcccttg tccagatagc ccagtagctg
     6661 acattcatcc ggggtcagca ccgtttctgc ggactggctt tctacgtaat ggtttcttag
     6721 acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     6781 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     6841 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
     6901 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
     6961 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
     7021 gagagttttc gccccgaaga acgttttcca atgatgagca ctttcaaagt tctgctatgt
     7081 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
     7141 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
     7201 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
     7261 cttctgacaa ctatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     7321 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     7381 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
     7441 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
     7501 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
     7561 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
     7621 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
     7681 gctgagatag gtgcctcact gattaagcat tggtaattcg aaatgaccga ccaagcgacg
     7741 cccaaccggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     7801 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     7861 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     7921 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     7981 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     8041 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     8101 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     8161 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     8221 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     8281 tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc
     8341 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     8401 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatttc
     8461 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     8521 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttatagtccg
     8581 gaaatacagg aacgcacgct ggatggccct tcgctgggat ggtgaaacca tgaaaaatgg
     8641 cagcttcagt ggattaagtg ggggtaatgt ggcctgtacc ctctggttgc ataggtattc
     8701 atacggttaa aatttatcag gcgcgattgc ggcagttttt cgggtggttt gttgccattt
     8761 ttacctgtct gctgccgtga tcgcgctgaa cgcgttttag cggtgcgtac aattaaggga
     8821 ttatggtaaa tccacttact gtctgccctc gtagccatcg agataaaccg cagtactccg
     8881 gccacgatgc gtccggcgta gaggatcgag atcttcaata ttggccatta gccatattat
     8941 tcattggtta tatagcataa atcaatattg gctattggcc attgcatacg ttgtatctat
     9001 atcataatat gtacatttat attggctcat gtccaatatg accgccatgt tggcattgat
     9061 tattgactag ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg
     9121 agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc
     9181 gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt
     9241 gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc
     9301 atatgccaag tccgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
     9361 cccagtacat gaccttacgg gactttccta cttggcagta catctacgta ttagtcatcg
     9421 ctattaccat ggtgatgcgg ttttggcagt acaccaatgg gcgtggatag cggtttgact
     9481 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
     9541 atcaacggga ctttccaaaa tgtcgtaata accccgcccc gttgacgcaa atgggcggta
     9601 ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatcacta
     9661 gaagctttat tgcggtagtt tatcacagtt aaattgctaa cgcagtcagt gcttctgaca
     9721 caacagtctc gaacttaagc tgcagaagtt ggtcgtgagg cactgggcag gtaagtatca
     9781 aggttacaag acaggtttaa ggagaccaat agaaactggg cttgtcgaga cagagaagac
     9841 tcttgcgttt ctgataggca cctattggtc ttactgacat ccactttgcc tttctctcca
     9901 caggtgtcca ctcccagttc aattacagct cttaaggcta gagtattaat acgactcact
     9961 atagggctag cgctcaccat ggtcttcaca ctcgaagatt tcgttgggga ctggcgacag
    10021 acagccggct acaacctgga ccaagtcctt gaacagggag gtgtgtccag tttgtttcag
    10081 aatctcgggg tgtccgtaac tccgatccaa aggattgtcc tgagcggtga aaatgggctg
    10141 aagatcgaca tccatgtcat catcccgtat gaaggtctga gcggcgacca aatgggccag
    10201 atcgaaaaaa tttttaaggt ggtgtaccct gtggatgatc atcactttaa ggtgatcctg
    10261 cactatggca cactggtaat cgacggggtt acgccgaaca tgatcgacta tttcggacgg
    10321 ccgtatgaag gcatcgccgt gttcgacggc aaaaagatca ctgtaacagg gaccctgtgg
    10381 aacggcaaca aaattatcga cgagcgcctg atcaaccccg acggctcc
//
