LOCUS       sequence-437056-        6477 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of full-length human COP1. N-terminal HiBiT 
            tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6477)
  AUTHORS   Ackloo S, Li F, Szewczyk M, Seitova A, Loppnau P, Zeng H, Xu J, 
            Ahmad S, Arnautova YA, Baghaie AJ, Beldar S, Bolotokova A, Centrella
            PA, Chau I, Clark MA, Cuozzo JW, Dehghani-Tafti S, Disch JS, Dong A,
            Dumas A, Feng JA, Ghiabi P, Gibson E, Gilmer J, Goldman B, Green SR,
            Guie MA, Guilinger JP, Harms N, Herasymenko O, Houliston S, 
            Hutchinson A, Kearnes S, Keefe AD, Kimani SW, Kramer T, Kutera M, 
            Kwak HA, Lento C, Li Y, Liu J, Loup J, Machado RAC, Mulhern CJ, 
            Perveen S, Righetto GL, Riley P, Shrestha S, Sigel EA, Silva M, 
            Sintchak MD, Slakman BL, Taylor RD, Thompson J, Torng W, 
            Underkoffler C, von Rechenberg M, Walsh RT, Watson I, Wilson DJ, 
            Wolf E, Yadav M, Yazdi AK, Zhang J, Zhang Y, Santhakumar V, Edwards 
            AM, Barsyte-Lovejoy D, Schapira M, Brown PJ, Halabelian L, 
            Arrowsmith CH
  TITLE     A Target Class Ligandability Evaluation of WD40 Repeat-Containing 
            Proteins.
  JOURNAL   J Med Chem. 2025 Jan 23;68(2):1092-1112. doi: 
            10.1021/acs.jmedchem.4c02010. Epub 2024 Nov 4.
  PUBMED    39495097
REFERENCE   2  (bases 1 to 6477)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acs.jmedchem.4c02010"; journalName: "J Med Chem"; date: 
            "2025-01-23- 23"; volume: "68"; issue: "2"; pages: "1092-1112"
COMMENT     Sequence Label: pBiT3.1-N_COP1:1-731
FEATURES             Location/Qualifiers
     source          1..6477
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    24..105
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     enhancer        338..641
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        642..845
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     796..816
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     intron          981..1113
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     1096..1115
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     primer_bind     1157..1176
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1157..1175
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    3540..3661
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3551..3570)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    complement(3776..3809)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        3819..3866
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             3898..4692
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3952..3971)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4562..4581
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     rep_origin      complement(4850..5438)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4930..4949)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    5564..5612
                     /note="synthetic polyadenylation signal"
     CDS             complement(5636..6034)
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     regulatory      6031..6040
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(6065..6422)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6079..6214
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     enhancer        6231..6422
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     6379..6399
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 aagacgcgtt aattaagacg tcggcagtga aaaaaatgct ttatttgtga aatttgtgat
       61 gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc
      121 attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaaag caagtaaaac
      181 ctctacaaat gtggtatggc tgattatgat ccgcggccgc aatacgtcga cgttatcagc
      241 tgacttcgta cgagagccta ggattatggc gcgccactag ttattaatag taatcaatta
      301 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      361 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      421 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      481 ctgcccactt ggcagtacat caagtgtatc atatgccaag tccgccccct attgacgtca
      541 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttacgg gactttccta
      601 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      661 acaccaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      721 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaata
      781 accccgcccc gttgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      841 gagctggttt agtgaaccgt cagatcacta gaatctttat tgcggtagtt tatcacagtt
      901 aaattgctaa cgcagtcagt gcttctgaca caacagtctc gaacttaagc tgcagaagtt
      961 ggtcgtgagg cactgggcag gtaagtatca aggttacaag acaggtttaa ggagaccaat
     1021 agaaactggg cttgtcgaga cagagaagac tcttgcgttt ctgataggca cctattggtc
     1081 ttactgacat ccactttgcc tttctctcca caggtgtcca ctcccagttc aattacagct
     1141 cttaaggcta gagtattaat acgactcact ataggggtta gcgatcgcca tggtgagcgg
     1201 ctggcggctg ttcaagaaga ttagcgggag ctccggtggc tcgagcggta tgtctggtag
     1261 ccgccaggcc gggtcgggct ccgctgggac aagccccggg tcctcggcgg cctcctcggt
     1321 gacttccgcc tcctcgtctt tatcctcttc cccgtcgccg ccttccgtgg cggtttcggc
     1381 ggcagcgctg gtgtccggcg gggtggccca ggccgccggc tcgggcggcc tcgggggccc
     1441 ggtgcggcct gtgttggtgg cgcccgccgt atcgggtagc ggcggcgggg cggtgtccac
     1501 gggcctgtcc cggcacagct gcgcggccag gcccagcgcc ggcgtaggag gcagcagctc
     1561 cagcctaggc agcggcagca ggaagcgacc tctcctcgcc cccctctgca acgggctcat
     1621 caactcctac gaggacaaaa gcaacgactt cgtatgcccc atctgctttg atatgattga
     1681 agaagcatac atgacaaaat gtggccacag cttttgctac aagtgtattc atcagagttt
     1741 ggaggacaat aatagatgtc ccaagtgtaa ctatgttgtg gacaatattg accatctgta
     1801 tcctaatttc ttggtgaatg aactcattct taaacagaag caaagatttg aggaaaagag
     1861 gttcaaattg gaccactcag tgagtagcac caatggccac aggtggcaga tatttcaaga
     1921 ttggttggga actgaccaag ataaccttga tttggccaat gtcaatctta tgttggagtt
     1981 actagtgcag aagaagaaac aactggaagc agaatcacat gcagcccaac tacagattct
     2041 tatggaattc ctcaaggttg caagaagaaa taagagagag caactggaac agatccagaa
     2101 ggagctaagt gttttggaag aggatattaa gagagtggaa gaaatgagtg gcttatactc
     2161 tcctgtcagt gaggatagca cagtgcctca atttgaagct ccttctccat cacacagtag
     2221 tattattgat tccacagaat acagccaacc tccaggtttc agtggcagtt ctcagacaaa
     2281 gaaacagcct tggtataata gcacgttagc atcaagacga aaacgactta ctgctcattt
     2341 tgaagacttg gagcagtgtt acttttctac aaggatgtct cgtatctcag atgacagtcg
     2401 aactgcaagc cagttggatg aatttcagga atgcttgtcc aagtttactc gatataattc
     2461 agtacgacct ttagccacat tgtcatatgc tagtgatctc tataatggtt ccagtatagt
     2521 ctctagtatt gaatttgacc gggattgtga ctattttgcg attgctggag ttacaaagaa
     2581 gattaaagtc tatgaatatg acactgtcat ccaggatgca gtggatattc attaccctga
     2641 gaatgaaatg acctgcaatt cgaaaatcag ctgtatcagt tggagtagtt accataagaa
     2701 cctgttagct agcagtgatt atgaaggcac tgttatttta tgggatggat tcacaggaca
     2761 gaggtcaaag gtctatcagg agcatgagaa gaggtgttgg agtgttgact ttaatttgat
     2821 ggatcctaaa ctcttggctt caggttctga tgatgcaaaa gtgaagctgt ggtctaccaa
     2881 tctagacaac tcagtggcaa gcattgaggc aaaggctaat gtgtgctgtg ttaaattcag
     2941 cccctcttcc agataccatt tggctttcgg ctgtgcagat cactgtgtcc actactatga
     3001 tcttcgtaac actaaacagc caatcatggt attcaaagga caccgtaaag cagtctctta
     3061 tgcaaagttt gtgagtggtg aggaaattgt ctctgcctca acagacagtc agctaaaact
     3121 gtggaatgta gggaaaccat actgcctacg ttccttcaag ggtcatatca atgaaaaaaa
     3181 ctttgtaggc ctggcttcca atggagatta tatagcttgt ggaagtgaaa ataactctct
     3241 ctacctgtac tataaaggac tttctaagac tttgctaact tttaagtttg atacagtcaa
     3301 aagtgttctc gacaaagacc gaaaagaaga tgatacaaat gaatttgtta gtgctgtgtg
     3361 ctggagggca ctaccagatg gggagtccaa tgtgctgatt gctgctaaca gtcagggtac
     3421 aattaaggtg ctagaattgg tataatctag aggtcaagct tcgggggttt aaacctgcag
     3481 gcatgcaagc tgatttggct gctgccaccg ctgagcaagg ccgcttcgag cagacatgat
     3541 aagatacatt gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat
     3601 ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt
     3661 taacaacaac aattgcattc attttatgtt tcaggttcag ggggagatgt gggaggtttt
     3721 tttaagcaag taaaacctct acaaatgtgg taaaatctta attaagactt ctaatataac
     3781 ttcgtatagc atacattata cgaagttatg gttccactgt tgacaattaa tcatcggcat
     3841 agtatatcgg catagtataa tacgacaagg tgaggaacta aacccaggag gcagatcatg
     3901 attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc
     3961 tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg
     4021 caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag
     4081 gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc
     4141 gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat
     4201 ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg
     4261 cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc
     4321 gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag
     4381 catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgcgcat gcccgacggc
     4441 gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc
     4501 cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata
     4561 gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc
     4621 gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac
     4681 gagttcttct gattcgaaat gaccgaccaa gcgacgccca accggtatca gctcactcaa
     4741 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     4801 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     4861 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     4921 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     4981 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     5041 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     5101 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     5161 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     5221 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     5281 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     5341 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     5401 gcaagcagca gattacgcgc agaaaaaaag gatttcaaga agatcctttg atcttttcta
     5461 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     5521 caaaaaggat cttcacctag atccttttat agtccggaat cgtcacacaa aaaaccaaca
     5581 cacagatgta atgaaaataa agatatttta ttgcggccat cgtgatggct agagtttagc
     5641 cctcccacac ataaccagag ggcagcaatt cacgaatccc aactgccgtc ggctgtccat
     5701 cactgtcctt cactatggct ttgatcccag gatgcagatc gagaagcacc tgtcggcacc
     5761 gtccgcaggg gctcaagatg cccctgttct catttccgat cgcgacgata caagtcaggt
     5821 tgccagctgc cgcagcagca gcagtgccca gcaccacgag ttctgcacaa ggtcccccag
     5881 taaaatgata tacattgaca ccagtgaaga tgcggccgtc gctagagaga gctgcgctgg
     5941 cgacgctgta gtcttcagag atggggatgc tgttgattgt agccgttgct ctttcaatga
     6001 gggtggattc ttcttgagac aaaggcttgg ccatggtggc gctagtgtca gaagaatcga
     6061 gctttttgca aaagcctagg cctccaaaaa agcctcctca ctacttctgg aatagctcag
     6121 aggccgaggc ggcctcggcc tctgcataaa taaaaaaaat tagtcagcca tggggcggag
     6181 aatgggcgga actgggcgga gttaggggcg ggatgggcgg agttaggggc gggactatgg
     6241 ttgctgacta attgagatgc atgctttgca tacttctgcc tgctggggag cctggggact
     6301 ttccacacct ggttgctgac taattgagat gcatgctttg catacttctg cctgctgggg
     6361 agcctgggga ctttccacac cctaactgac acacattcca cagctggttc tttccgcctc
     6421 agaaggtacc taaccaagtt cctctttcag aggttatttc aggccatggt gctgcgc
//
