LOCUS       sequence-437060-       13733 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Tn7 transposase (tnsABCD) expression plasmid for Rhodobacter 
            sphaeroides.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13733)
  AUTHORS   Hall AN, Hall BW, Kinney KJ, Olsen GG, Banta AB, Noguera DR, Donohue
            TJ, Peters JM
  TITLE     Tools for genetic engineering and gene expression control in 
            Novosphingobium aromaticivorans and Rhodobacter sphaeroides.
  JOURNAL   Appl Environ Microbiol. 2024 Sep 26:e0034824. doi: 
            10.1128/aem.00348-24.
  PUBMED    39324814
REFERENCE   2  (bases 1 to 13733)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Environ Microbiol. 2024 Sep 26:e0034824. doi: 10.1128/aem.00348-24."
COMMENT     Sequence Label: pJMP8045
FEATURES             Location/Qualifiers
     source          1..13733
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(join(110..115,116..132,133..138))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     primer_bind     complement(163..179)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(163..179)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(280..299)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      942..1028
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(1199..1990)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      5762..6350
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6251..6270
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6504..6521
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     6780..6796
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     mobile_element  13108..13332
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
ORIGIN
        1 aaaaaaacag tcataacaag ccatgaaaac cgccactgcg ccgttaccac cgctgcgttc
       61 ggtcaaggtt ctggaccagt tgcgtgagcg catacgctac ttgcattaca gtttacgaac
      121 cgaacaggct tatgtcaact gggttcgtga attcgtaatc atgtcatagc tgtttcctgc
      181 ttggcgagat tttcaggagc taaggaagct aaaatggaga aaaaaatcac tggatatacc
      241 accgttgata tatcccaatg gcatcgtaaa gaacattttg aggcatttca gtcagttgct
      301 caatgtacct ataaccagac cgttcagctg gatattacgg cctttttaaa gaccgtaaag
      361 aaaaataagc acaagtttta tccggccttt attcacattc ttgcccgcct gatgaatgct
      421 catccggaat ttcgtatggc aatgaaagac ggtgagctgg tgatatggga tagtgttcac
      481 ccttgttaca ccgttttcca tgagcaaact gaaacgtttt catcgctctg gagtgaatac
      541 cacgacgatt tccggcagtt tctacacata tattcgcaag atgtggcgtg ttacggtgaa
      601 aacctggcct atttccctaa agggtttatt gagaatatgt ttttcgtctc agccaatccc
      661 tgggtgagtt tcaccagttt tgatttaaac gtggccaata tggacaactt cttcgccccc
      721 gttttcacca tgcatgggca aatattatac gcaaggcgac aaggtgctga tgccgctggc
      781 gattcaggtt catcatgccg tttgtgatgg cttccatgtc ggcagaatgc ttaatgaatt
      841 acaacagtac tgcgatgagt ggcagggcgg ggcgtaattt ttttaaggca gttattggtg
      901 cccttaaacg cctggggtaa tgactctcta gcttgaggca tcaaataaaa cgaaaggctc
      961 agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctcctgagta
     1021 ggacaaatcc gccgctagga gcttgcggcc ctggcgtaat agcgaagagg cccgcaccga
     1081 tcgcccttcc caacagttgc gcagcctgaa tggcgaatgc gatttattca acaaagccgc
     1141 cgtcccgtca agtcagcgta atgctctgcc agtgttacaa ccaattaacc aattctgatt
     1201 atttgccgac taccttggtg atctcgcctt tcacgtagtg gacaaattct tccaactgat
     1261 ctgcgcgcga ggccaagcga tcttcttctt gtccaagata agcctgtcta gcttcaagta
     1321 tgacgggctg atactgggcc ggcaggcgct ccattgccca gtcggcagcg acatccttcg
     1381 gcgcgatttt gccggttact gcgctgtacc aaatgcggga caacgtaagc actacatttc
     1441 gctcatcgcc agcccagtcg ggcggcgagt tccatagcgt taaggtttca tttagcgcct
     1501 caaatagatc ctgttcagga accggatcaa agagttcctc cgccgctgga cctaccaagg
     1561 caacgctatg ttctcttgct tttgtcagca agatagccag atcaatgtcg atcgtggctg
     1621 gctcgaagat acctgcaaga atgtcattgc gctgccattc tccaaattgc agttcgcgct
     1681 tagctggata acgccacgga atgatgtcgt cgtgcacaac aatggtgact tctacagcgc
     1741 ggagaatctc gctctctcca ggggaagccg aagtttccaa aaggtcgttg atcaaagctc
     1801 gccgcgttgt ttcatcaagc cttacggtca ccgtaaccag caaatcaata tcactgtgtg
     1861 gcttcaggcc gccatccact gcggagccgt acaaatgtac ggccagcaac gtcggttcga
     1921 gatggcgctc gatgacgcca actacctctg atagttgagt cgatacttcg gcgatcaccg
     1981 cttccctcat aacacccctt gtattactgt ttatgtaagc agacagtttt attgttcatg
     2041 atgatatatt tttatcttgt gcaatgtaac atcagagatt ttgagacaca acgtggcttt
     2101 gttgaataaa tcgaactttt gctgagttga aggatcagat cacgcatctt cccgacaacg
     2161 cagaccgttc cgtggcaaag caaaagttca aaatcaccaa ctggtccacc tacaacaaag
     2221 ctctcatcaa ccgtggctcc ctcgaggctc cgcgccctcg atccgaccgc cccccacaag
     2281 gctggggcgg atcgggggcg cacggcctga agggtgcagg ggaaccctgc cgcacgcaaa
     2341 ctcgcagagt ttgcatagtg tgcgccattc agataccttc atggcaggcc ccaccgtgga
     2401 ttttgctgtc atggttcgat tcatcacgac gcccgcaacg ccgactgcac agcggcccgc
     2461 accacgaacg cctgcgcagc aggaagctgc ggctgaactg aaggctgcgc gaactgccga
     2521 agcagcccgc cgcctcgaca cccggcgcaa ggtggtgctc gggggcgcgc tcctggcact
     2581 ggcagaacgt gatgcagcag cagccgcgat gctggcccgg ctgaagcaga tgctgacccg
     2641 cccagcagat cggaagctgt tcgcggatct acccgatggc tgacggtgca atcttccacc
     2701 tcgatgtgcg cacggtcagc cggtcagatg gccgctcggc tgtcgccgct gccgcctatc
     2761 ggtcggccag ccgtctgcac gatgcacgca ccggcctgac ccacgacttc gcgcgcaagc
     2821 ggggcgtctt gacaaccttc atcgccgccc cggacggctg cgactggatc acagacaggg
     2881 acacgctctg gaacgcggct gaggcggcag agaagcgcaa gaacagcacc gtggcccgtg
     2941 agtggctggt ggccctccct gacgctctgg atgctacaca gcgggccgac ctggcgcggg
     3001 cgctggcggt cgaactggcg cagcggttcg gggtggccgt ggatgtggca atccacgcag
     3061 cttcgggcga gggcgaccag cggaaccatc acgcgcattt gctgaccacg acgcgcaccg
     3121 caggcccgga cgggctgggc gacaagaccc gcgttctgga tgcagccaaa acggggggcg
     3181 cagaaatcta cgccatgcgg gcttggtggg cgggcacggt caacgatgca ctcgcggccg
     3241 cacaaagctc ggcccgcgtc gatcaccgcc gcatagccgt gatcgccgcc gatgcgcggg
     3301 ccgaggccga ggcgctggag aaacaggcca aagccgtcga aaccctgaac gccaagcccg
     3361 ccgacgccgg ggggctgtgg aagggccttg gcagcgccgc ccgcgcaata tgggaaggcg
     3421 gcatgaccgc cctcaaatcg accgccgaga cagccaacga gctgcgggag aaggccaacg
     3481 agctgcgaca gaaggcagcc cgctacaccc gcgccaccga acctcaccac ggccccgtcc
     3541 tgacggcctt cttgcggcgc atggctcccg tctgggcgga gcaggccaag gccgaggaag
     3601 cccggagagc cgcgcaggag gccgcacagc gcgcactgat ggccgagagg gctgaacagg
     3661 cccggaaggc caaggaagcc tctgaggccg ctcgccgggc cgctacggca gcgcgagagg
     3721 ccgaggaagc ccgcgaacgc tgcgcccgcg aggtgttgcc gctcatccgc cgcgcccggc
     3781 acgacccgat caccgccgaa gtgatcaagc gggagggcat cgacctcgac cgccccgacc
     3841 aacaggtggc ccgcgacccg atctggctca tggccggacg ccaagatcag gcgcacccgg
     3901 tctggctgat cgtccacgcc tacaccgccg cgcgcgacag gcacgcgaac ctgctggcga
     3961 gaatcgaagc caaaaccgca gcccagatcg ccgctgccgc caaaccaaag cccaagcctg
     4021 ccagtcccgc cccgaccgaa cccgaacccg atccagaccc accgccccgg cgggattggg
     4081 gcgggccgtc tggaccctga tccacccccc attctcagca actccgagcc agccagaggc
     4141 gctggctcgg ggcatctgga cccccttcct caccccaaat tttcacgaaa aaggggcgca
     4201 gcccttcttg ttcaataagt tctataagtt caggcgaaaa tcgcctttca ttttcaatgg
     4261 cttgcgcgcc tatcggggag aaatggggcc gcaaaaggga gaaatggggc cgcaaaaggg
     4321 agaaatgggg ccgatagcgg ctgttgacag ggggatattt tgccccctac atttctcccc
     4381 atgacaaaga ccacgcaaat cgccgctgat cgagcctatg acgaggccaa aaccgtgctg
     4441 cccgcagagg tggcgcgggg ggtctacatg cgcaacgccc ccagcctcgc cgccctcaag
     4501 ctgatgcacc tgctgatcgg taaggcgggc gggcgtatgg ccgaacccat tcggcacgaa
     4561 atccgcctct cagacatccg caagatcgac gggatgaaga accatgacag ggccagcctc
     4621 accccccttt tcgcggaatt gtcggcggct gtcctgaccc atgatgaccc cgaaaaaatg
     4681 gtcgtgacca ttggcggctt gctggacttc gcccgcatcg actaccgcca cgaagtcagc
     4741 aacgatctgc tggtgtcgtg gtattttggc ggggccttcc gcgagatggc cgagaagtcc
     4801 tgccactggg cgatcctcga ccggcagacc gtttttgccc tgtccagcaa gtattcgatc
     4861 ctgctgttcc agcacatcgc cagcctcgtg aacctcgatc acgtcgccag caagaccttc
     4921 accgtggccg aactgcgggc cgtgctaggg gtgccgcacg gaaagatgga gcggtggaac
     4981 gacgtgaacc gctttgcgct gacgcccgcg attgccgaga tcaaccagac ctcgcgcctg
     5041 atcctgaccg cgaccccgaa caagatcggg cggacggtcg ccagcgtgac aatcacgtgg
     5101 gcggagaagc ctcccgaggg caagcaggaa gccaagcgcg aactggaccg ccccaaggtg
     5161 ggccgagagg cgcggcgcaa cggctcagcc gagaccgtgg cgcgggcctt cccgagctcg
     5221 ggcagcatcg agttcgatga ccactggaag gccctcaagc gggcggcggg ctgcaacatg
     5281 gacaacacca tgattgccga gaagttccgg gcgtggtgcg ccagcaaggg gctttccctc
     5341 gacggccgaa acatcgagca ggcgttcagc aacttctgcg ccacagtggg caaggtctga
     5401 ggccgcgccc gctgccagtg acccgccgcc gccgaccgtg agcgggccgc agcccttcca
     5461 gcgccgccca agagggcggc aagccccggt ctgccccctc gtcgcccgca aagcgggcag
     5521 ggggcagacc ggggcgctgg aacctttggg ctgctttgac ggccagacag cccccgccgc
     5581 gcaagcgcgc cattccccca cccgtggccg ggcgggggaa tggtaaagca tgtgggggca
     5641 gtccagccgt caatccgcgc tccgcaccac ggcgcctcga gcaacacctt cttcacgagg
     5701 cagacctctc gacggagttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     5761 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     5821 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     5881 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
     5941 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     6001 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     6061 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     6121 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     6181 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     6241 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     6301 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     6361 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     6421 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     6481 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gaagctcgca
     6541 cattcagcag cgtttttcag cgcgttttcg atcaacgttt caatgttggt atcaacacca
     6601 ggtttaactt tgaacttatc ggcactgacg gttactgatt ttgaactttt gctttgccac
     6661 ggaacggtct gcgttgtcgg gaagatgcgt gatctgatcc ttcaactcag caaaagttcg
     6721 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ggccgcgcgg
     6781 taaaacgacg gccagtgcca agcttgcatg cctgcaggtc gactctagag gatcccgatt
     6841 attttggctc gttgaagatc cgatgcttca aactcaatca cacgatccgc cttatttatt
     6901 tgctttgttg tactgttaac caccttagct cgacttaata aaggcaaatg agtaaatcga
     6961 gtccatttcc gttcttgcat tggattaaac caaacaaaaa tacgccactc cttatgaccc
     7021 gagttacgga ataaatgacc aatatgtaca acctgcacat tgtcattaaa tgtagctagc
     7081 ctaaccactc actcttcccc ataaaccatt tccaagaatc tttgtgcttc ctcagttatc
     7141 cgctctttag ataacgttgc acttcttaat aatcgccagc gcctaagctc aacatcttcc
     7201 tgtttaagct taataaaagc ttggctaatc cgtctaattt gataatcttc cacactctct
     7261 gagtaacgct ttaagcaaag cgctaccaaa ggcagtttct gtagattttt tgcaagagag
     7321 gttccgttag gagtttgctt taacagccag ctcgatgtcg ctcttgggtg atcaaggcta
     7381 ctatctagac gctttatgat tcttaatagt tgtcgtacag caattcgatc tctttggttc
     7441 caatcaactc taggggcggg tgccagacgc tcttgttgat gctgttgatt ccagtgaact
     7501 agccaatccc tgtcatgtcg gtaaagccaa gcgtatagca ccccaccctc taaagactgt
     7561 cttgccgcct taattccttg gtatttatgc actagttgct gccagtcttt atgcttaaca
     7621 gataaatctt cagagttagg ttgcacagac tggctaacag gtcttgtcgt tatagagtgc
     7681 tcagtaagag cacttgcctg ctgtagcgct tctataaccg ttagttttgg caataaggct
     7741 tgccacacaa tactatgctg taaataacta aaggcttttc tatgcttacg gaatatactt
     7801 ttcagccaac acgtgtcctt gttctctgcc aactttaaat ccagtttctc tagtgcctca
     7861 tcactaaaag tctgcctcac tctctccgcc accaagtcat gacgaatgtg cttgcttttg
     7921 gttagcccta gatcctgcgc taagcgctga taaaacagcg tccactgctc aaggcttggg
     7981 gaaagctctt gcgctcgtgg agcatctaac agaggggcta tataagcagc tagtgctgtt
     8041 aattgagata gggagtcttt ggggtagtct gaaagcagct cagtatgacc caaagcccaa
     8101 aattgatgtc ggtgatcatc tacagctcta tcaaagaaga ctaaagcacc gtgttttgga
     8161 caatatggca aagcgggcaa ataccaatct cgttgccaaa aggcttcccc atacctattt
     8221 agctgaagag caacgcaatc agggcagtat ctaaagcggt tatcgctctt aactctagaa
     8281 gcagcgactc ctagcattaa atgcaccgca ccttgcgctt ggtactccat taaccgaata
     8341 gcttcgtctc ggcgctcctt gcctacaaac ggagcatata aagggaataa ggtatgctca
     8401 taaataagct gctgaacagc gtaacgtcct gtttgatgta gatgtcttgc tatcacacct
     8461 aaatgcgagg gcagacctaa ggtagcgacc accttgcggt tgccatacac ctcatccaac
     8521 agctgcttag gactaacaat cccttgataa acgcctgccc gtgcaatagt gctataaatc
     8581 agctcattcg agtacggaac aggaaagttt ctcatggcta acctgcttgt ttaaataagc
     8641 tcgccatatc cactattacc cctttccctt ttaaccgttc atgcatggtt ttttcaggtt
     8701 ggcgttgtga atagatataa cgtaaatccg tatcaggcaa gctatcccag tcgctgggct
     8761 tgactacctt tatagccgaa acctttttac tcttggaggg cttttctagt tctgacacta
     8821 ccgtttcgcc ttccatcaac cactgcaaaa caagaggcag taacttttgt cttgtcatcg
     8881 ttggattctg gctaaacgct tttttaatag tgggaattaa caggcttgaa tcgtaatcct
     8941 ctttcagcat cagatataaa tgacgctgat cttcggtatc taactcttga agggcttttt
     9001 cttctggtgt ttgttcttgt atcgctgcga tatctagctg aagttggatt aaccgtttat
     9061 caatctcggg aacgactaga tcagaataac gagcaatgcg ttctgggata cccgagcgta
     9121 atgcctctag catggggtgc acaggcttta actcatcttg atacacttgc cgcaataaac
     9181 cagcggtaat acgctcattg cctaaagcta gcgcacggag ctgagcgagt acaaaaagtt
     9241 ttactacaat gtccatcact ccttggctta gctcatacca cacatcacgg acctcatccg
     9301 ataacagcgc atctttgcgt tgtaaaagct gtaattgcca aagattatcc gtaaaagcga
     9361 tccactcttg attgggcttt ccacgttgcg tttgttgtat aggatcccag aatatagctc
     9421 caaaccctgc ccctctacgt gcagaccgca aatcagcctc aaaaatctct cgtgctttag
     9481 gggtaccaat caacatcact ggtacgccaa taatattcac catcgtcaca aaaaagttca
     9541 gcatctcttg agatccaccc gaacgagagc ggcttaaatg ctgaatttca tcaataacca
     9601 acaaccctaa agcatgtgca ttggctattt gcgacatcaa agccaacatg gtttctatac
     9661 catgacgttt taagccataa cgacgctcat agttcgagcc caaggctcga tccaacgctc
     9721 tgaaaaaatt caagcagatt tcttttagcg aaccattatg cgagcagtct attttcaaat
     9781 acaccacctg ctctacattg agttcacgat ggtaaatcac ctgaggatac gtggctagaa
     9841 tacgatgaag agaggtcgtc ttcccactac cagaacaacc aattaacaat aagctttgtg
     9901 ccgtagatcg tgcctcctca aagcgaaatg tctccaactc tcccgtttga acacgctcat
     9961 aaccattttg taaatgcttt tgtaaatctc ctgttttagg atttctgcct acgtagccac
    10021 ctcgaatcat gaccgaaata cgctcactta gtagcaaatg cgtacctaat ggctgaaaat
    10081 agtcatctgg aatacgacaa atggtatgag ctctgataac acgggacttt tgcaagtcag
    10141 aggaagtaag ctgtaaagag gatttcagtg atgcagcact attcactgac tcttgtaatg
    10201 gtggtaaggc ctcgataaaa ggattatcac gataagcctc tacccccgta tcacgataaa
    10261 ctgcttgaat ccgggtagca ctcatgactc atccttttct ggtggatcct gaaataattc
    10321 aggcacgtat gtgggtaggc tgtaatcttc ttgatcatcc gcttccactg cgttgaaagg
    10381 aataacttta gcctcatcac ctgaagagct tggcttcaaa tgctccgcac gttttttacg
    10441 ctccgaggtc acggcttctt ttttattagt tttaatctgc ttaatgcgtg ttgatttggg
    10501 ctcagtagta ctgggcgtta acttattcgc tttctgaatg gtttgctgaa taaacgcctc
    10561 aagctccctg cgtttagtta actcatcctg cttcgcattg gctttattgt gtttttcttg
    10621 tgcttgtata tcccaaacct cccaaaatga gagaccttta aactgccgac tacgttccgt
    10681 cagattacag cgccaaaata cacggctgcc aacttgcgga aacaaataaa tcgtatcaac
    10741 cagcactggg tcataagccg cttctaaatg ttgaggtcta gctatatcag tgctccgctg
    10801 caaccaaccc tcacgcagaa tctctgaccc cgagtaatac aaaccccaca aattaacgcc
    10861 aaatgaagaa atagagacct ttcggcgagg cagtaacgct actcgcaact gctcttgctc
    10921 cacagccctt aaactacctg tacgatgctg cataccccat tgccatagct ggacaggaat
    10981 agacggtaaa tctgtaggaa aatcagcatc tcgatcgtat ttatccatca ccagatggtt
    11041 atttctgaat aagatcgtac gcaaaataat ttgtgtgaac tcaaataccg acagagatgc
    11101 atctaaccta tagtctgttt caccatggct tttgatccga ctgccctcta caatgccagg
    11161 tgcaaaggac ttaaactcgg cttgtagtgt tctaaaagtg ctttccacta tgcctttagc
    11221 atcgccacgt ctaggtggag cactttccac tcgcacatta aaactagaaa ctaaggcttc
    11281 gacctgatga ctcattaatt cgccacggtc cgctagcaac acatctggca aacctacaca
    11341 cggccagtct gagctactaa tctcaatatc atgctgggca caaatggccg ttttgtcaga
    11401 gcaagcattt acaaaagcct gcatcgccac cacataagac ggattttcaa agccgatata
    11461 aaagcccgtg atcatccgac taaacacatc aatcacaatg taaagcgttg gtcttcctat
    11521 gattttttgg cgatcatgat gatccactaa ataaatatca gcaatcgtgg catcaatctc
    11581 ataacgactc ccagggccta acgcctgaga agtggctgta ctacttaagg gtcgtacgtc
    11641 ttttttatat acccctgctt taactctaga ctttaagcgc tgagctttag ggtattctcg
    11701 atcataaaaa taacgaaact gacgtagtgt tgggtaatcc tcttggggaa tgcgaggaaa
    11761 atactgagca aacaagtcca caaatcgtct ataggcaacg gtggtctttg tacctttttg
    11821 atttaacagg tgcttttcta tggtcaacct aaaaaggcgt tcaatctcgg gcgttacctt
    11881 ggttccttca cccttaccat attctctggc tcggccaatc tttgctgttc ctgtcgctga
    11941 acgtctttcc cctggtgcac cgctgttttt gtagtcagga attaatgcat taggcgtttg
    12001 accacgctgc cagtaacggc gtaacaactt ataaactgta gccttagtaa ccttatgttc
    12061 ttggaccaca tgctcaacga gttcgcttct gactttaggg tcgaaacgat ccttactatt
    12121 aataatagga agaatttttc gataatcctc ctcgcgcttc tggaagctga cagaatctac
    12181 agacggctct tctaaatcaa gatgtacata agggtcatca gttctaacta agcggccttc
    12241 atctaaatac tgcattagca acaactcagc cctagcttgt ggaactcctt tatcagcgct
    12301 tatttgcatc cagacaactt ggccatcctc tatagccaaa atgcgatacg gatcattatc
    12361 aaatagcaca acctcattaa tttgccacat agcgcaactc ctccatattc actacttggc
    12421 taatacagag atctgcacac ttatttgccc tgaaagactt ataaatattg aacttaataa
    12481 aaccatttgc ggttaaggct cgtatctcac tcaatgtttt gcctaactcc aaatcataag
    12541 caatatcaac ctgcttacag acattgataa tgttttcatc tcctttttct tgcaggatat
    12601 gggccaatgg ggatagttgt gctaaaagct ccgcagaaac ttcttctgtt ttcactgaat
    12661 aaagccattc aatattttct tttactacgg gatttatttc tttatcagta aaaatgaacc
    12721 aaggaatttg cttttgctgc caatagcgac gctctagttc tagtttttct aaggtacgct
    12781 cgtcttgtaa ggctgctgca ggtttgactt gaatagcaaa ctgctcaaaa ggaccatctt
    12841 tgcagtccac taaaaaatca gtagacataa cctgatctac accacgaata acaggatgct
    12901 taataccact atctattgca atctgcctgg tatcactagg taataagggg aactgctcgc
    12961 gtatatctag cacgctgctc tcccactcaa gactgagaaa aacagcaagc tctaagtcag
    13021 atagcaaatg atggactcgt cccgtcttat gagaataaat acggtgggaa cgacctgaag
    13081 aaggaacttc ttgtactgtt agccatggaa tatagtcttt accatgccct tggccacgcc
    13141 cctctttaat acgacgggca atttgcactt cagaaaatga agagtttgct ttagccataa
    13201 caaaagtcca gtatgctttt tcacagcata actggactga tttcagttta caactattct
    13261 gtctagttta agactttatt gtcatagttt agatctattt tgttcagttt aagactttat
    13321 tgtccgccca cacccgctta cgcagggcat ccatttatta ctcaaccgta accgattttg
    13381 ccaggttacg cggctggtca acgtcggtgc ctttgatcag cgcgacatgg taagccagca
    13441 gctgcagcgg aacggtgtag aagatcggtg caatcacctc ttccacatgc ggcatctcga
    13501 ctctagagga tccccgggtc gaatccttct tgtgaatcta ttatggcgac aagcaaatcg
    13561 agctctgact gcctacccca caacaactat cagaaagcac cagcacaacg gctgcctaac
    13621 tttgttttag ggcgactgcc ctgctgcgta acatcgttgc tgctccataa catcaaacat
    13681 cgacccacgg cgtaacgcgc ttgctgcttg gatgcccgag gcatagactg tac
//
