LOCUS       sequence-437149-        3247 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3247)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 3247)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pENTR-SLC66A1_STOP
FEATURES             Location/Qualifiers
     source          1..3247
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(883..982)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1039..1055)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1039..1055)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1052..1074)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(1227..1815)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1307..1326)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1951..2766)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(1951..2766)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     2674..2693
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     2758..2777
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     2887..2903
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2887..2903
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     terminator      2904..2950
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3064..3086
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3078..3095
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3079..3095
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3148..3247
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 ccaccatggt ctggaaaaag ctcggcagtc gcaatttttc ctcttgcccg tctggctcta
       61 ttcagtggat ttgggacgtt ttgggggaat gtgcacagga cggttgggac gaagcaagcg
      121 tggggctcgg ccttatttct atcttgtgtt tcgccgcctc cacctttcct cagttcatca
      181 aagcctataa aacgggcaac atggatcagg ctctgagcct ttggttcctc ctgggctgga
      241 ttggagggga ctcttgtaat ctcattggct cttttttggc cgatcagctt cctctgcaga
      301 cttataccgc agtctattat gtccttgcag atttggtcat gctgactctg tacttttact
      361 acaagttcag gacgaggcct agcctgctgt ctgcccccat taattccgtt ctcctcttct
      421 tgatgggtat ggcatgtgcc acgcccttac tgagtgcagc tggcccggta gctgcaccgc
      481 gagaggcttt ccgaggtcgg gcattactca gcgtagagtc cggcagcaag ccctttacaa
      541 gacaagaggt gattgggttt gttatcggca gcatcagcag cgtactgtac ctcctgagca
      601 gactgccgca aattcgaacc aatttcctga ggaagtccac acaggggatc tcatactccc
      661 tgtttgcgct ggtcatgctg ggcaacactt tgtacggctt gtccgtcctg ctcaaaaacc
      721 ccgaagaagg gcagtctgaa ggcagctatt tgctccatca cttaccatgg ttggtggggt
      781 cactgggggt cctccttctc gacactataa ttagtatcca attcttagtg tatcgcaggt
      841 caactgctgc atctgagctc gagccactcc tgccttcatg agacccagct ttcttgtaca
      901 aagttggcat tataagaaag cattgcttat caatttgttg caacgaacag gtcactatca
      961 gtcaaaataa aatcattatt tgaggctagg tggaggctca gtgatgataa gtctgcgatg
     1021 gtggatgcat gtgtcatggt catagctgtt tcctgtgtga aattgttatc cgctcagagg
     1081 gcacaatcct attccgcgct atccgacaat ctccaagaca ttaggtggag ttcagttcgg
     1141 cgtatggcat atgtcgctgg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     1201 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     1261 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     1321 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     1381 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     1441 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     1501 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     1561 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     1621 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     1681 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     1741 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     1801 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tctattcaac
     1861 aaagccgccg tcccgtcaag tcagcgtaaa tgggtagggg gcttcaaatc gtccgctctg
     1921 ccagtgttac aaccaattaa caaattctga ttagaaaaac tcatcgagca tcaaatgaaa
     1981 ctgcaattta ttcatatcag gattatcaat accatatttt tgaaaaagcc gtttctgtaa
     2041 tgaaggagaa aactcaccga ggcagttcca taggatggca agatcctggt atcggtctgc
     2101 gattccgact cgtccaacat caatacaacc tattaatttc ccctcgtcaa aaataaggtt
     2161 atcaagtgag aaatcaccat gagtgacgac tgaatccggt gagaatggca aaagcttatg
     2221 catttctttc cagacttgtt caacaggcca gccattacgc tcgtcatcaa aatcactcgc
     2281 atcaaccaaa ccgttattca ttcgtgattg cgcctgagcg agacgaaata cgcgatcgct
     2341 gttaaaagga caattacaaa caggaatcga atgcaaccgg cgcaggaaca ctgccagcgc
     2401 atcaacaata ttttcacctg aatcaggata ttcttctaat acctggaatg ctgttttccc
     2461 ggggatcgca gtggtgagta accatgcatc atcaggagta cggataaaat gcttgatggt
     2521 cggaagaggc ataaattccg tcagccagtt tagtctgacc atctcatctg taacatcatt
     2581 ggcaacgcta cctttgccat gtttcagaaa caactctggc gcatcgggct tcccatacaa
     2641 tcgatagatt gtcgcacctg attgcccgac attatcgcga gcccatttat acccatataa
     2701 atcagcatcc atgttggaat ttaatcgcgg cctcgagcaa gacgtttccc gttgaatatg
     2761 gctcataaca ccccttgtat tactgtttat gtaagcagac agttttattg ttcatgatga
     2821 tatattttta tcttgtgcaa tgtaacatca gagattttga gacacaacgt ggctttcccc
     2881 cgccgctcta gaactagtgg atccaaataa aacgaaaggc tcagtcgaaa gactgggcct
     2941 ttcgttttat ctgttgtttg tcgcattata cgagacgtcc aggttgggat acctgaaaca
     3001 aaacccatcg tacggccaag gaagtctcca ataactgtga tccaccacaa gcgccagggt
     3061 tttcccagtc acgacgttgt aaaacgacgg ccagtcatgc ataatccgca cgcatctgga
     3121 ataaggaagt gccattccgc ctgacctcaa ataatgattt tattttgact gatagtgacc
     3181 tgttcgttgc aacagattga tgagcaatgc ttttttataa tgccaacttt gtacaaaaaa
     3241 gcaggct
//
