LOCUS       sequence-437157-        3406 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3406)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 3406)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pENTR-TMEM144
FEATURES             Location/Qualifiers
     source          1..3406
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(1042..1141)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1198..1214)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1198..1214)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1211..1233)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(1386..1974)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1466..1485)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(2110..2925)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(2110..2925)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     2833..2852
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     2917..2936
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     3046..3062
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3046..3062
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     terminator      3063..3109
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3223..3245
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3237..3254
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3238..3254
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3307..3406
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 ccaccatgtc aaacaacggg gcagacctga cctttgggta cattagttgt tttgtggcga
       61 tcctcttatt cggcagtaac tttgtcccac tgaagaaatt tgataccggg gacggtatgt
      121 ttctgcagtg ggtcctgtgc gccgccatat ggcttgtcgc cctggtggtg aatttgatct
      181 tacactgtcc gaaattttgg cctttcgcga tgctgggtgg ctgcatctgg gctacaggga
      241 atatagccgt ggtgccaatc atcaagacca ttggactcgg cctggggata ctcatctggg
      301 gctctttcaa tgctctgaca gggtgggcca gctcccgctt tggctggttt ggccttgatg
      361 ctgaggaagt ctccaacccc ctcttaaatt atataggggc tggtttgtcc gttgtgtctg
      421 ctttcatctt tcttttcatt aagtccgaaa tccctaataa tacgtgttcc atggatacga
      481 cccctctcat caccgagcac gtaatcaata ccactcaaga tccgtgtagc tgggtggaca
      541 aactttccac cgtgcaccac aggatcgttg gatgcagttt ggcagtaatt tcaggcgtcc
      601 tctacgggag tacctttgtg cctattatct acatcaaaga tcacagtaaa aggaatgatt
      661 ctatctacgc cggagccagt cagtatgatt tggattatgt tttcgcccat tttagcggaa
      721 ttttccttac ttcaactgtg tattttctcg cgtactgtat cgctatgaag aatagtccaa
      781 agctctaccc agaggctgtt cttccaggct tcttgagcgg agtcctgtgg gccatcgcaa
      841 cctgctgctg gttcattgcc aatcatagcc tgtccgccgt ggtctcattt ccgatcataa
      901 ccgcgggtcc cggttttata gctgcaatgt ggggcatttt catgtttaag gaaattaagg
      961 gtctccaaaa ttatttgctt atgattctcg cattttgcat aattctgaca ggcgctctgt
     1021 gcacggcttt ctcaaagatc gacccagctt tcttgtacaa agttggcatt ataagaaagc
     1081 attgcttatc aatttgttgc aacgaacagg tcactatcag tcaaaataaa atcattattt
     1141 gaggctaggt ggaggctcag tgatgataag tctgcgatgg tggatgcatg tgtcatggtc
     1201 atagctgttt cctgtgtgaa attgttatcc gctcagaggg cacaatccta ttccgcgcta
     1261 tccgacaatc tccaagacat taggtggagt tcagttcggc gtatggcata tgtcgctgga
     1321 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     1381 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     1441 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     1501 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     1561 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     1621 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     1681 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     1741 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     1801 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     1861 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     1921 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     1981 cctttgatct tttctacggg gtctgacgct ctattcaaca aagccgccgt cccgtcaagt
     2041 cagcgtaaat gggtaggggg cttcaaatcg tccgctctgc cagtgttaca accaattaac
     2101 aaattctgat tagaaaaact catcgagcat caaatgaaac tgcaatttat tcatatcagg
     2161 attatcaata ccatattttt gaaaaagccg tttctgtaat gaaggagaaa actcaccgag
     2221 gcagttccat aggatggcaa gatcctggta tcggtctgcg attccgactc gtccaacatc
     2281 aatacaacct attaatttcc cctcgtcaaa aataaggtta tcaagtgaga aatcaccatg
     2341 agtgacgact gaatccggtg agaatggcaa aagcttatgc atttctttcc agacttgttc
     2401 aacaggccag ccattacgct cgtcatcaaa atcactcgca tcaaccaaac cgttattcat
     2461 tcgtgattgc gcctgagcga gacgaaatac gcgatcgctg ttaaaaggac aattacaaac
     2521 aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca tcaacaatat tttcacctga
     2581 atcaggatat tcttctaata cctggaatgc tgttttcccg gggatcgcag tggtgagtaa
     2641 ccatgcatca tcaggagtac ggataaaatg cttgatggtc ggaagaggca taaattccgt
     2701 cagccagttt agtctgacca tctcatctgt aacatcattg gcaacgctac ctttgccatg
     2761 tttcagaaac aactctggcg catcgggctt cccatacaat cgatagattg tcgcacctga
     2821 ttgcccgaca ttatcgcgag cccatttata cccatataaa tcagcatcca tgttggaatt
     2881 taatcgcggc ctcgagcaag acgtttcccg ttgaatatgg ctcataacac cccttgtatt
     2941 actgtttatg taagcagaca gttttattgt tcatgatgat atatttttat cttgtgcaat
     3001 gtaacatcag agattttgag acacaacgtg gctttccccc gccgctctag aactagtgga
     3061 tccaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt
     3121 cgcattatac gagacgtcca ggttgggata cctgaaacaa aacccatcgt acggccaagg
     3181 aagtctccaa taactgtgat ccaccacaag cgccagggtt ttcccagtca cgacgttgta
     3241 aaacgacggc cagtcatgca taatccgcac gcatctggaa taaggaagtg ccattccgcc
     3301 tgacctcaaa taatgatttt attttgactg atagtgacct gttcgttgca acagattgat
     3361 gagcaatgct tttttataat gccaactttg tacaaaaaag caggct
//
