LOCUS       sequence-437179-        5608 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  cdGreen2.1 (optimized for Pseudomonas GC-content) expressed from a 
            constitutive promoter (Internal Lab ID: UJ11239).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5608)
  AUTHORS   Kaczmarczyk A, van Vliet S, Jakob RP, Teixeira RD, Scheidat I, 
            Reinders A, Klotz A, Maier T, Jenal U
  TITLE     A genetically encoded biosensor to monitor dynamic changes of 
            c-di-GMP with high temporal resolution.
  JOURNAL   Nat Commun. 2024 May 9;15(1):3920. doi: 10.1038/s41467-024-48295-0.
  PUBMED    38724508
REFERENCE   2  (bases 1 to 5608)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2024-05-9"; volume: "15(1):3920. doi"; pages: " 
            10.1038/s41467-024-48295-0"
COMMENT     Sequence Label: pBBR15.2-2H12.D11opt
FEATURES             Location/Qualifiers
     source          1..5608
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    267..288
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1032..1053)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1293..1312)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     CDS             2216..2749
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     CDS             complement(3152..3814)
                     /codon_start=1
                     /product="replication protein for the broad-host-range 
                     plasmid pBBR1 from Bordetella bronchiseptica"
                     /label=pBBR1 Rep
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
     rep_origin      3815..4586
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
ORIGIN
        1 accttcggga gcgcctgaag cccgttctgg acgccctggg gccgttgaat cgggatatgc
       61 aggccaaggc cgccgcgatc atcaaggccg tgggcgaaaa gctgctgacg gaacagcggg
      121 aagtccagcg ccagaaacag gcccagcgcc agcaggaacg cgggcgcgca catttccccg
      181 aaaagtgcca cctgacgtct aagaacatgt tgcagctggc acgacaggtt tcccgactgg
      241 aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag
      301 gctttatagc tagcttgaca tcccatagag tgatagagat actgagcaca tcagcatatg
      361 aaagtgggtc ttaataataa taattccgaa ggaggaggtg atccatgaat tcggaaccgc
      421 cgccgaagct ggtcctggac ctggaacgcc tggcgcacgt cccgcaggaa aaggcgggcc
      481 cgctgcagcg ctatgccgcc accatccagt cgcagcgggg cgactacaac ggcaaggtcc
      541 tgtcgatccg gccggcggac ctgcgcaccc tggccgtcat ctatgaccag tcgccgtcgg
      601 tcctgaccga gcagctgatc tcgtggggcg tcctggatgc cgatgcgcgc cgcgccgtcg
      661 cgcatgaaga gaacccgaag aacatgccgg tgccgtacaa cgtgttcatc atggccgaca
      721 agcagaagaa cggcatcaag gccaacttca agatccgcca caacatcgag gatggcggcg
      781 tccagctggc ctaccactac cagcagaaca ccccgatcgg cgacggcccg gtcctgctgc
      841 cggacaacca ttacctgggc gtccagtcga agctgtcgaa ggacccgaac gagaagcgcg
      901 accacatggt gctgctggag ttcgtcaccg ccgcgggcat caccctgggc atggacgagc
      961 tgtacaaggg cggcaccggc ggctcgggcg tcagcaaggg cgaagaactg ttcaccggcg
     1021 tcgtcccgat cctggtcgag ctggacggcg acgtcaacgg ccacaagttc tcggtcagcg
     1081 gcgaaggcga gggcgacgcc acctatggca agctgaccct gaagttcatc tgcaccaccg
     1141 gcaagctgcc cgtgccgtgg ccgacgctgg tcaccacgct gacctatggc gtgcagtgct
     1201 tctcgcgcta cccggaccac atgaagcagc acgatttctt caagagcgcc atgccggaag
     1261 gctacatcca ggaacggacc atcttcttca aggacgacgg caactacaag acccgcgccg
     1321 aggtcaagtt cgaaggcgac accctggtca accgcatcga gctgaagggc atcgacttca
     1381 aggaagatgg caacatcctg ggccacaagc tggaatacaa catgctgtcg gagcccgaac
     1441 cgccgccgaa gctggtgctg gatctggaac ggctggccca tgtgccgcag gaaaaggcgg
     1501 gcccgctgca gcggtatgcg gcgacgatcc agagccagcg cggcgattat aacggcaagg
     1561 tgctgagcat ccggccggcg gatctgcgga cgctggcggt gatctacgat cagagcccga
     1621 gcgtgctgac ggaacagctg atctcctggg gcgtgctgga cgcggatgcc cggcgcgcgg
     1681 tcgcccacga ggaaaactaa actagtaaaa aaggatccag cttcacgctg ccgcaagcac
     1741 tcagggcgca agggctgcta aaggaagcgg aacacgtaga aagccagtcc gcagaaacgg
     1801 tgctgacccc ggatgaatgt cagctactgg gctatctgga caagggaaaa cgcaagcgca
     1861 aagagaaagc aggtagcttg cagtgggctt acatggcgat agctagactg ggcggtttta
     1921 tggacagcaa gcgaaccgga attgccagct ggggcgccct ctggtaaggt tgggaagccc
     1981 tgcaaagtaa actggatggc tttcttgccg ccaaggatct gatggcgcag gggatcaaga
     2041 tccatgcgct cacgcaactg gtccagaacc ttgaccgaac gcagcggtgg taacggcgca
     2101 gtggcggttt tcatggcttg ttatgactgt ttttttgtac agtctattcc tcgggcatcc
     2161 aagcagcaag cgcgttacgc cgtgggtcga tgtttgatgt tatggagcag caacgatgtt
     2221 acgcagcagc aacgatgtta cgcagcaggg cagtcgccct aaaacaaagt taggtggctc
     2281 aagtatgggc atcattcgca catgtaggct cggccctgac caagtcaaat ccatgcgggc
     2341 tgctcttgat cttttcggtc gtgagttcgg agacgtagcc acctactccc aacatcagcc
     2401 ggactccgat tacctcggga acttgctccg tagtaagaca ttcatcgcgc ttgctgcctt
     2461 cgaccaagaa gcggttgttg gcgctctcgc ggcttacgtt ctgcccaagt ttgagcagcc
     2521 gcgtagtgag atctatatct atgatctcgc agtctccggc gagcaccgga ggcagggcat
     2581 tgccaccgcg ctcatcaatc tcctcaagca tgaggccaac gcgcttggtg cttatgtgat
     2641 ctacgtgcaa gcagattacg gtgacgatcc cgcagtggct ctctatacaa agttgggcat
     2701 acgggaagaa gtgatgcact ttgatatcga cccaagtacc gccacctaac aattcgttca
     2761 agccgagatc ggcttcccgg ccgcggagtt gttacttgtt cggttcaggg cagggtcgtt
     2821 aaatagccgc ttatgtctat tgctggttta ccggtttatt gactaccgga agcagtgtga
     2881 ccgtgtgctt ctcaaatgcc tgaggccagt ttgctcaggc tctccccgtg gaggtaataa
     2941 ttgacgatat gatcatttat tctgcctccc agagcctgat aaaaacggtg aatccgttag
     3001 cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa
     3061 cgcggggagg cagacaaggt atagggcggc gaggcggcta cagccgatag tctggaacag
     3121 cgcacttacg ggttgctgcg caacccaagt gctaccggcg cggcagcgtg acccgtgtcg
     3181 gcggctccaa cggctcgcca tcgtccagaa aacacggctc atcgggcatc ggcaggcgct
     3241 gctgcccgcg ccgttcccat tcctccgttt cggtcaaggc tggcaggtct ggttccatgc
     3301 ccggaatgcc gggctggctg ggcggctcct cgccggggcc ggtcggtagt tgctgctcgc
     3361 ccggatacag ggtcgggatg cggcgcaggt cgccatgccc caacagcgat tcgtcctggt
     3421 cgtcgtgatc aaccaccacg gcggcactga acaccgacag gcgcaactgg tcgcggggct
     3481 ggccccacgc cacgcggtca ttgaccacgt aggccgacac ggtgccgggg ccgttgagct
     3541 tcacgacgga gatccagcgc tcggccacca agtccttgac tgcgtattgg accgtccgca
     3601 aagaacgtcc gatgagcttg gaaagtgtct tctggctgac caccacggcg ttctggtggc
     3661 ccatctgcgc cacgaggtga tgcagcagca ttgccgccgt gggtttcctc gcaataagcc
     3721 cggcccacgc ctcatgcgct ttgcgttccg tttgcaccca gtgaccgggc ttgttcttgg
     3781 cttgaatgcc gatttctctg gactgcgtgg ccatgcttat ctccatgcgg taggagtgcc
     3841 gcacggttgc ggcaccatgc gcaatcagct gcaactcttc ggcagcgcga caacaattat
     3901 gcgttgcgta aaagtggcag tcaattacag attttcttta acctacgcaa tgagctattg
     3961 cggggggtgc cgcaatgagc tgttgcgtac cccccttttt taagttgttg atttttaagt
     4021 ctttcgcatt tcgccctata tctagttctt tggtgcccaa agaagggcac ccctgcgggg
     4081 ttcccccacg ccttcggcgc ggctccccct ccggcaaaaa gtggcccctc cggggcttgt
     4141 tgatcgactg cgcggccttc ggccttgccc aaggtggcgc tgcccccttg gaacccccgc
     4201 actcgccgcc gtgaggctcg gggggcaggc gggcgggctt cgcccttcga ctgcccccac
     4261 tcgcataggc ttgggtcgtt ccaggcgcgt caaggccaag ccgctgcgcg gtcgctgcgc
     4321 gagccttgac ccgccttcca cttggtgtcc aaccggcaag cgaagcgcgc aggccgcagg
     4381 ccggaggctt ttccccagag aaaattaaaa aaattgatgg ggcaaggccg caggccgcgc
     4441 agttggagcc ggtgggtatg tggtcgaagg ctgggtagcc ggtgggcaat ccctgtggtc
     4501 aagctcgtgg gcaggcgcag cctgtccatc agcttgtcca gcagggttgt ccacgggccg
     4561 agcgaagcga gccagccggt ggccgctcgc ggccatcgtc cacatatcca cgggctggca
     4621 agggagcgca gcgaccgcgc agggcgaagc ccggagagca agcccgtagg gcgccgcagc
     4681 cgccgtaggc ggtcacgact ttgcgaagca aagtctagtg agtatactca agcattgagt
     4741 ggcccgccgg aggcaccgcc ttgcgctgcc cccgtcgagc cggttggaca ccaaaaggga
     4801 ggggcaggca tggcggcata cgcgatcatg cgatgcaaga agctggcgaa aatgggcaac
     4861 gtggcggcca gtctcaagca cgcctaccgc gagcgcgaga cgcccaacgc tgacgccagc
     4921 aggacgccag agaacgagca ctgggcggcc agcagcaccg atgaagcgat gggccgactg
     4981 cgcgagttgc tgccagagaa gcggcgcaag gacgctgtgt tggcggtcga gtacgtcatg
     5041 acggccagcc cggaatggtg gaagtcggcc agccaagaac agcaggcggc gttcttcgag
     5101 aaggcgcaca agtggctggc ggacaagtac ggggcggatc gcatcgtgac ggccagcatc
     5161 caccgtgacg aaaccagccc gcacatgacc gcgttcgtgg tgccgctgac gcaggacggc
     5221 aggctgtcgg tcaaggagtt catcgacaac aaagcgcaga tgacccgcga ccagaccacg
     5281 tttgcggccg ctgtggccga tctagggctg caacggggca tcgagggcag caaggcacgt
     5341 cacacgcgca ttcaggcgtt ctacgaggcc ctggagcggc caccagtggg ccacgtcacc
     5401 atcagcccgc aagcggtcga gccacgcgcc tatgcaccgc agggattggc cgaaaagctg
     5461 ggaatctcaa agcgcgttga gacgccggaa gccgtggccg accggctgac aaaagcggtt
     5521 cggcaggggt atgagcctgc cctacaggcc gccgcaggag cgcgtgagat gcgcaagaag
     5581 gccgatcaag cccaagagac ggcccgag
//
