LOCUS       sequence-437292-        5203 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Firefly luciferase with mouse liver DNase hypersensitive site # 5898
            cloned into KpnI/XhoI-digested pPromALB_FireflyLuc.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5203)
  AUTHORS   Chang TY, Waxman DJ
  TITLE     HDI-STARR-seq: Condition-specific enhancer discovery in mouse liver 
            in vivo.
  JOURNAL   BMC Genomics. 2024 Dec 24;25(1):1240. doi: 
            10.1186/s12864-024-11162-9.
  PUBMED    39716078
REFERENCE   2  (bases 1 to 5203)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Genomics."; date: "2024-12-24"; volume: "25(1):1240. doi"; pages: " 
            10.1186/s12864-024-11162-9"
COMMENT     Sequence Label: pTYC1_pPromALB_enh5898_FireflyLuc
FEATURES             Location/Qualifiers
     source          1..5203
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    857..978
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(868..887)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     922..941
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(1226..1243)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1397..1985)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1477..1496)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2185..2976,2977..3045))
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     polyA_signal    3150..3198
                     /note="synthetic polyadenylation signal"
     misc_feature    3212..3303
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     3252..3271
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     regulatory      4358..4367
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4364..5203,1..813)
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
ORIGIN
        1 aagattcaat ctgccctgct ggtgcccaca ctatttagct tcttcgctaa gagcactctc
       61 atcgacaagt acgacctaag caacttgcac gagatcgcca gcggcggggc gccgctcagc
      121 aaggaggtag gtgaggccgt ggccaaacgc ttccacctac caggcatccg ccagggctac
      181 ggcctgacag aaacaaccag cgccattctg atcacccccg aaggggacga caagcctggc
      241 gcagtaggca aggtggtgcc cttcttcgag gctaaggtgg tggacttgga caccggtaag
      301 acactgggtg tgaaccagcg cggcgagctg tgcgtccgtg gccccatgat catgagcggc
      361 tacgttaaca accccgaggc tacaaacgct ctcatcgaca aggacggctg gctgcacagc
      421 ggcgacatcg cctactggga cgaggacgag cacttcttca tcgtggaccg gctgaagagc
      481 ctgatcaaat acaagggcta ccaggtagcc ccagccgaac tggagagcat cctgctgcaa
      541 caccccaaca tcttcgacgc cggggtcgcc ggcctgcccg acgacgatgc cggcgagctg
      601 cccgccgcag tcgtcgtgct ggaacacggt aaaaccatga ccgagaagga gatcgtggac
      661 tatgtggcca gccaggttac aaccgccaag aagctgcgcg gtggtgttgt gttcgtggac
      721 gaggtgccta aaggactgac cggcaagttg gacgcccgca agatccgcga gattctcatt
      781 aaggccaaga agggcggcaa gatcgccgtg taataattct agagtcgggg cggccggccg
      841 cttcgagcag acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag
      901 tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata
      961 agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg
     1021 gaggtgtggg aggtttttta aagcaagtaa aacctctaca aatgtggtaa aatcgataag
     1081 gatccgtcga ccgatgccct tgagagcctt caacccagtc agctccttcc ggtgggcgcg
     1141 gggcatgact atcgtcgccg cacttatgac tgtcttcttt atcatgcaac tcgtaggaca
     1201 ggtgccggca gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     1261 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     1321 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     1381 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     1441 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     1501 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     1561 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     1621 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     1681 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     1741 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     1801 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
     1861 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     1921 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     1981 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     2041 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     2101 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagcggcc
     2161 gcaaatgcta aaccactgca gtggttacca gtgcttgatc agtgaggcac cgatctcagc
     2221 gatctgccta tttcgttcgt ccatagtggc ctgactcccc gtcgtgtaga tcactacgat
     2281 tcgtgagggc ttaccatcag gccccagcgc agcaatgatg ccgcgagagc cgcgttcacc
     2341 ggcccccgat ttgtcagcaa tgaaccagcc agcagggagg gccgagcgaa gaagtggtcc
     2401 tgctactttg tccgcctcca tccagtctat gagctgctgt cgtgatgcta gagtaagaag
     2461 ttcgccagtg agtagtttcc gaagagttgt ggccattgct actggcatcg tggtatcacg
     2521 ctcgtcgttc ggtatggctt cgttcaactc tggttcccag cggtcaagcc gggtcacatg
     2581 atcacccata ttatgaagaa atgcagtcag ctccttaggg cctccgatcg ttgtcagaag
     2641 taagttggcc gcggtgttgt cgctcatggt aatggcagca ctacacaatt ctcttaccgt
     2701 catgccatcc gtaagatgct tttccgtgac cggcgagtac tcaaccaagt cgttttgtga
     2761 gtagtgtata cggcgaccaa gctgctcttg cccggcgtct atacgggaca acaccgcgcc
     2821 acatagcagt actttgaaag tgctcatcat cgggaatcgt tcttcggggc ggaaagactc
     2881 aaggatcttg ccgctattga gatccagttc gatatagccc actcttgcac ccagttgatc
     2941 ttcagcatct tttactttca ccagcgtttc ggggtgtgca aaaacaggca agcaaaatgc
     3001 cgcaaagaag ggaatgagtg cgacacgaaa atgttggatg ctcatactcg tcctttttca
     3061 atattattga agcatttatc agggttacta gtacgtctct caaggataag taagtaatat
     3121 taaggtacgg gaggtattgg acaggccgca ataaaatatc tttattttca ttacatctgt
     3181 gtgttggttt tttgtgtgaa tcgatagtac taacatacgc tctccatcaa aacaaaacga
     3241 aacaaaacaa actagcaaaa taggctgtcc ccagtgcaag tgcaggtgcc agaacatttc
     3301 tctggcctaa ctggccggta ccggctgttc tcatcatgcg catgtagctg aggaagacca
     3361 gctcccctag cctccactat gcagtaccag attgactgga gtgtgcttgt tggaaggttt
     3421 tactttggca ggtacaaatt cacaggcctg ggtgtgtgga caacagccaa ctatgaccta
     3481 cagcaccgtg accccctgcc cactgccctt cacctcgcct gggccattga tcatggtaac
     3541 atggaccaag agggcagcgg ggtgcagggg tccctccagt gcagagcatg ctggcctgac
     3601 tcccgagcac cacctgctgt tgcctggggc aaactctggg tttggaggac tcccaggtaa
     3661 gagaaaccct gtgaccccaa caaccctgta ggtgaagagg aggttcacaa ggtgaactct
     3721 ggtgacttca cagcaccaag agcttgatgg gtcagagcca cctccaacca agtcattgtc
     3781 ctcaccagag accttctgta acagtgcctg cagctcaact gacacagctt gctcatagtc
     3841 tagttggtaa ggtgcttgta gctcagccaa tacagtactt gtagcccagt gagtaaggtg
     3901 cttgcagctc agttggtaga gtgcttgtca agcacacagg aggcccatca gatcacccca
     3961 taagcaaggt agctcgagga tatcaagatc tggcctcggc ggccaagctt tgctcaaatg
     4021 ggagacaaag agattaagct cttatgtaaa atttgctgtt ttacataact ttaatgaatg
     4081 gacaaagtct tgtgcatggg ggtgggggtg gggttagagg ggaacagctc cagatggcaa
     4141 acatacgcaa gggatttagt caaacaactt tttggcaaag atggtatgat tttgtaatgg
     4201 ggtaggaacc aatgaaatgc gaggtaagta tggttaataa tctacagtta ttggttaaag
     4261 aagtatatta gagcgagtct ttctgcacac agatcacctt cctatcaacc ccactagcct
     4321 ctggcaaaaa gcttggcaat ccggtactgt tggtaaagcc accatggaag atgccaaaaa
     4381 cattaagaag ggcccagcgc cattctaccc actcgaagac gggaccgccg gcgagcagct
     4441 gcacaaagcc atgaagcgct acgccctggt gcccggcacc atcgccttta ccgacgcaca
     4501 tatcgaggtg gacattacct acgccgagta cttcgagatg agcgttcggc tggcagaagc
     4561 tatgaagcgc tatgggctga atacaaacca tcggatcgtg gtgtgcagcg agaatagctt
     4621 gcagttcttc atgcccgtgt tgggtgccct gttcatcggt gtggctgtgg ccccagctaa
     4681 cgacatctac aacgagcgcg agctgctgaa cagcatgggc atcagccagc ccaccgtcgt
     4741 attcgtgagc aagaaagggc tgcaaaagat cctcaacgtg caaaagaagc taccgatcat
     4801 acaaaagatc atcatcatgg atagcaagac cgactaccag ggcttccaaa gcatgtacac
     4861 cttcgtgact tcccatttgc cacccggctt caacgagtac gacttcgtgc ccgagagctt
     4921 cgaccgggac aaaaccatcg ccctgatcat gaacagtagt ggcagtaccg gattgcccaa
     4981 gggcgtagcc ctaccgcacc gcaccgcttg tgtccgattc agtcatgccc gcgaccccat
     5041 cttcggcaac cagatcatcc ccgacaccgc tatcctcagc gtggtgccat ttcaccacgg
     5101 cttcggcatg ttcaccacgc tgggctactt gatctgcggc tttcgggtcg tgctcatgta
     5161 ccgcttcgag gaggagctat tcttgcgcag cttgcaagac tat
//
