LOCUS       sequence-437295-        5462 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Firefly luciferase with mouse liver DNase hypersensitive site # 
            29774 cloned into KpnI/XhoI-digested pPromALB_FireflyLuc.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5462)
  AUTHORS   Chang TY, Waxman DJ
  TITLE     HDI-STARR-seq: Condition-specific enhancer discovery in mouse liver 
            in vivo.
  JOURNAL   BMC Genomics. 2024 Dec 24;25(1):1240. doi: 
            10.1186/s12864-024-11162-9.
  PUBMED    39716078
REFERENCE   2  (bases 1 to 5462)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Genomics."; date: "2024-12-24"; volume: "25(1):1240. doi"; pages: " 
            10.1186/s12864-024-11162-9"
COMMENT     Sequence Label: pTYC2_pPromALB_enh29774_FireflyLuc
FEATURES             Location/Qualifiers
     source          1..5462
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      1314..1323
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1320..2972
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     polyA_signal    3016..3137
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3027..3046)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3081..3100
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(3385..3402)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3556..4144)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3636..3655)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4344..5135,5136..5204))
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     polyA_signal    5309..5357
                     /note="synthetic polyadenylation signal"
     misc_feature    5371..5462
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     5411..5430
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
ORIGIN
        1 ggcctaactg gccggtacct tctacaggga gataactatg ttttacagct attagatttt
       61 agggttatgt gagtcaggta ccatatagct gcttctcttt gccctcgata aagaattcac
      121 ctgtattcac tcaggcaata agtattatat agtaaacttc tgacacagat tttaatccac
      181 aggtagtgta attattaact aaactcattt ttatttgaaa gtcacagcct gagtcatgaa
      241 atgttgactc tcagggcata gttgatcctg gtgaacccag ttgaactgaa gcctgaagag
      301 aagagtgtaa ggtacagggg acttgggttc cagcttaact gtactaccct cagtctgtgt
      361 gactctctaa ggcccagctt cctcagctct gaaaaagaaa gggttttatc atgatgggca
      421 aagctaattg agggactcca tagggataca tttggtgtga aacctctgga aatgaaacta
      481 tcaaaattct gtcaaagatg ggtagaacat tgcctaactc ttcctccttg tctctgcaga
      541 ggtgacattt gctcagtttt gttttgtttt tgtttttgtt ctcctgagta catcagagtc
      601 aaataaggac ttctgtgttg tgtgaagctg atgtcctagg aacaaaatca gggagctgta
      661 catttggagt aattaaagat ggggggttga ttttgtttgc cctctggata gcttcagtgc
      721 aaggcatagg tcaccgcaaa ggccaacaga tcttatctca aagctaagtt tttccatgta
      781 cgaggtacaa tatataacat gtgtggtatg ctcccagatt tcctttaccc ataaaaaatt
      841 agaaacaaaa cttcctgctt taagtgtttt tttttaaaga gggaatttaa cagtaaaata
      901 tacatattta cttagttaat tacttggcct cgaggatatc aagatctggc ctcggcggcc
      961 aagctttgct caaatgggag acaaagagat taagctctta tgtaaaattt gctgttttac
     1021 ataactttaa tgaatggaca aagtcttgtg catgggggtg ggggtggggt tagaggggaa
     1081 cagctccaga tggcaaacat acgcaaggga tttagtcaaa caactttttg gcaaagatgg
     1141 tatgattttg taatggggta ggaaccaatg aaatgcgagg taagtatggt taataatcta
     1201 cagttattgg ttaaagaagt atattagagc gagtctttct gcacacagat caccttccta
     1261 tcaaccccac tagcctctgg caaaaagctt ggcaatccgg tactgttggt aaagccacca
     1321 tggaagatgc caaaaacatt aagaagggcc cagcgccatt ctacccactc gaagacggga
     1381 ccgccggcga gcagctgcac aaagccatga agcgctacgc cctggtgccc ggcaccatcg
     1441 cctttaccga cgcacatatc gaggtggaca ttacctacgc cgagtacttc gagatgagcg
     1501 ttcggctggc agaagctatg aagcgctatg ggctgaatac aaaccatcgg atcgtggtgt
     1561 gcagcgagaa tagcttgcag ttcttcatgc ccgtgttggg tgccctgttc atcggtgtgg
     1621 ctgtggcccc agctaacgac atctacaacg agcgcgagct gctgaacagc atgggcatca
     1681 gccagcccac cgtcgtattc gtgagcaaga aagggctgca aaagatcctc aacgtgcaaa
     1741 agaagctacc gatcatacaa aagatcatca tcatggatag caagaccgac taccagggct
     1801 tccaaagcat gtacaccttc gtgacttccc atttgccacc cggcttcaac gagtacgact
     1861 tcgtgcccga gagcttcgac cgggacaaaa ccatcgccct gatcatgaac agtagtggca
     1921 gtaccggatt gcccaagggc gtagccctac cgcaccgcac cgcttgtgtc cgattcagtc
     1981 atgcccgcga ccccatcttc ggcaaccaga tcatccccga caccgctatc ctcagcgtgg
     2041 tgccatttca ccacggcttc ggcatgttca ccacgctggg ctacttgatc tgcggctttc
     2101 gggtcgtgct catgtaccgc ttcgaggagg agctattctt gcgcagcttg caagactata
     2161 agattcaatc tgccctgctg gtgcccacac tatttagctt cttcgctaag agcactctca
     2221 tcgacaagta cgacctaagc aacttgcacg agatcgccag cggcggggcg ccgctcagca
     2281 aggaggtagg tgaggccgtg gccaaacgct tccacctacc aggcatccgc cagggctacg
     2341 gcctgacaga aacaaccagc gccattctga tcacccccga aggggacgac aagcctggcg
     2401 cagtaggcaa ggtggtgccc ttcttcgagg ctaaggtggt ggacttggac accggtaaga
     2461 cactgggtgt gaaccagcgc ggcgagctgt gcgtccgtgg ccccatgatc atgagcggct
     2521 acgttaacaa ccccgaggct acaaacgctc tcatcgacaa ggacggctgg ctgcacagcg
     2581 gcgacatcgc ctactgggac gaggacgagc acttcttcat cgtggaccgg ctgaagagcc
     2641 tgatcaaata caagggctac caggtagccc cagccgaact ggagagcatc ctgctgcaac
     2701 accccaacat cttcgacgcc ggggtcgccg gcctgcccga cgacgatgcc ggcgagctgc
     2761 ccgccgcagt cgtcgtgctg gaacacggta aaaccatgac cgagaaggag atcgtggact
     2821 atgtggccag ccaggttaca accgccaaga agctgcgcgg tggtgttgtg ttcgtggacg
     2881 aggtgcctaa aggactgacc ggcaagttgg acgcccgcaa gatccgcgag attctcatta
     2941 aggccaagaa gggcggcaag atcgccgtgt aataattcta gagtcggggc ggccggccgc
     3001 ttcgagcaga catgataaga tacattgatg agtttggaca aaccacaact agaatgcagt
     3061 gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa
     3121 gctgcaataa acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg
     3181 aggtgtggga ggttttttaa agcaagtaaa acctctacaa atgtggtaaa atcgataagg
     3241 atccgtcgac cgatgccctt gagagccttc aacccagtca gctccttccg gtgggcgcgg
     3301 ggcatgacta tcgtcgccgc acttatgact gtcttcttta tcatgcaact cgtaggacag
     3361 gtgccggcag cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     3421 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     3481 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     3541 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     3601 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     3661 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     3721 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     3781 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     3841 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     3901 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     3961 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     4021 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     4081 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     4141 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     4201 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     4261 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagcggccg
     4321 caaatgctaa accactgcag tggttaccag tgcttgatca gtgaggcacc gatctcagcg
     4381 atctgcctat ttcgttcgtc catagtggcc tgactccccg tcgtgtagat cactacgatt
     4441 cgtgagggct taccatcagg ccccagcgca gcaatgatgc cgcgagagcc gcgttcaccg
     4501 gcccccgatt tgtcagcaat gaaccagcca gcagggaggg ccgagcgaag aagtggtcct
     4561 gctactttgt ccgcctccat ccagtctatg agctgctgtc gtgatgctag agtaagaagt
     4621 tcgccagtga gtagtttccg aagagttgtg gccattgcta ctggcatcgt ggtatcacgc
     4681 tcgtcgttcg gtatggcttc gttcaactct ggttcccagc ggtcaagccg ggtcacatga
     4741 tcacccatat tatgaagaaa tgcagtcagc tccttagggc ctccgatcgt tgtcagaagt
     4801 aagttggccg cggtgttgtc gctcatggta atggcagcac tacacaattc tcttaccgtc
     4861 atgccatccg taagatgctt ttccgtgacc ggcgagtact caaccaagtc gttttgtgag
     4921 tagtgtatac ggcgaccaag ctgctcttgc ccggcgtcta tacgggacaa caccgcgcca
     4981 catagcagta ctttgaaagt gctcatcatc gggaatcgtt cttcggggcg gaaagactca
     5041 aggatcttgc cgctattgag atccagttcg atatagccca ctcttgcacc cagttgatct
     5101 tcagcatctt ttactttcac cagcgtttcg gggtgtgcaa aaacaggcaa gcaaaatgcc
     5161 gcaaagaagg gaatgagtgc gacacgaaaa tgttggatgc tcatactcgt cctttttcaa
     5221 tattattgaa gcatttatca gggttactag tacgtctctc aaggataagt aagtaatatt
     5281 aaggtacggg aggtattgga caggccgcaa taaaatatct ttattttcat tacatctgtg
     5341 tgttggtttt ttgtgtgaat cgatagtact aacatacgct ctccatcaaa acaaaacgaa
     5401 acaaaacaaa ctagcaaaat aggctgtccc cagtgcaagt gcaggtgcca gaacatttct
     5461 ct
//
