LOCUS       sequence-437422-        7463 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  DiCas7-11 effector with Cas7.1 domain deletion mutation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7463)
  TITLE     CRISPR-Cas transgene repressible element
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7463)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: 1203O-CMV-DiCas7-11-Delta-7.1-bGHpolyA
FEATURES             Location/Qualifiers
     source          1..7463
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    877..898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(913..918,919..936,937..943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    951..967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     956..978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     975..991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     975..991
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    1062..1286
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     1275..1292
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(1315..1332)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     promoter        complement(5475..5678)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(5504..5524)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        5679..6058
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     6230..6249
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        6328..6432
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6433..6501,6502..7293)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6651..6670)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctcttcccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcatcctag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagctct agctagaggt
     1021 cgacggtatc gagccccagc tggttctttc cgcctcagaa gccatagagc ccaccgcatc
     1081 cccagcatgc ctgctattgt cttcccaatc ctcccccttg ctgtcctgcc ccaccccacc
     1141 ccccagaata gaatgacacc tactcagaca atgcgatgca atttcctcat tttattagga
     1201 aaggacagtg ggagtggcac cttccagggt caaggaaggc acgggggagg ggcaaacaac
     1261 agatggctgg caactagaag gcacagtcga ggctgatcag cgggtttaaa ctcaatggtg
     1321 atggtgatga tgaccggttt atgcccacgg tgtccacggc gtggtcagct tcttctgccg
     1381 atcttctttc ttgaattccc cgtctttgag ttcttcgtaa ccgctattcc cattaggatc
     1441 gtccttcttt cgcagcatgg ggtagcaaac ccggggtgct tggtcgccct caggaaacca
     1501 aagaagtttc ttaagatttt ctataaaatc gagctcgtcc cggaaccatt ccttcagttt
     1561 agcaaatcct tttcccaacc agttgggaat ttcggaattg ccattccgcc attgcttcca
     1621 atcttttctc aggcgcactg attcgacgtc aatttcaact gaaccaaatc ccatagactt
     1681 tgccattcca agtttatgcg ccagaccctt ttcgagttgc aggctgtgga tgagcaaacc
     1741 cagctcccat tctttcaggt tctcaaaggc aatttcgaag ctaaaggaat tcccgccggc
     1801 gagagcttct acagtgcggt tattagggct ttgttcaata gctttgcctg ttgtgggatg
     1861 gttcccgtcc ttaatggttt tccaggcgtg gtggtggacg taaaactttc tgccaggcac
     1921 tttaaactta ttgtcggatc ccggtatact ccaagtcggg cgaggccgtt ccaacaatga
     1981 gagcatcacc ggcccgccgt gaaacggatc gccgggattc ttgcctggga tcagccactc
     2041 cggatcgttt tcgagtgacg cgaatccaaa cctgactctg cccttgtaag atccggttcc
     2101 aaagagccgg caagcagggc agagtccttt ggggtggcgc aagaggagtc ttttctcggg
     2161 atatgcattc aaggcactga gatcaccatc ttccacccaa tctccatggc acggccgcag
     2221 atcggcgctc atacgtttgc cgatcatcct atcgtctaca gttcgggaaa tccgcactgg
     2281 gacgatgtcc tctacgtact tttcattatg tttgaaatac acaagatctc cggacttcag
     2341 cttttccacg ttttcccggg caactctaga ctgaaatacg ctctccggca ctgcttgagg
     2401 attgttgtta tagaccctga gcagttcttt gtatttgatt cttgctttct cggggatctc
     2461 gtattcctcg ttctccttca gatcgaagaa gaaggtttcg caatatttag ccatggtata
     2521 gtaattgccc ttatcatcct cacaacaaaa cacaggttca ttcttccttt tcctattctt
     2581 aaaatcgttc gtcctaattg ttttccagtc gttgggaact gatgggagtg ttccttgaaa
     2641 gttattgatc acatcccctt tcttgtcact aaactctact ttgctggggc cgacgacatg
     2701 aaggtagccc tctttacact cccatccgtc tgggtcgttc tcgggtatct cttgaaaggc
     2761 cttttggtat ctactatctc ttacccagca atccagtttc tcaccattct gtatgccttc
     2821 gagtttctcc tggtcaaaat tgtcaggttt atgccacaag tggaaagaag tgcagccatt
     2881 attggtgacc acgttaaaat aataagagcc attcttttgc tctataaatg tggcgatgcc
     2941 ttctttgcgt cttttccatt tattgtcttg tttcttctgg gggtgtttag caggatcaac
     3001 gaggctcagt cgtttaataa aacgcttgac ccacatccga acaggtttct caccagcaat
     3061 ttcttgctca tcaagaatgt caaaatgttt ctgcgttttg tcatagaaag gcacccttgc
     3121 agtttcggaa aacttttgta tatgtttccc atcggcggtc actcgacctg ggagaaagtc
     3181 ttgcagcaca ttctggtgat cagcatccat ccgccatgac aaccgtttag tctcatcaaa
     3241 tatcctgaag caagaatttg tcacagtttc gtagacacta ctcaccattc ctctcaattc
     3301 gctcccggga atcatgattt gtttatgaag tctgaagaaa gcatatgatt tatgatattc
     3361 gtccttttcc ttccgagctt ctttatccgc aggtctgaag aaatcgtcat tactagtatc
     3421 tgggacgatg aggggtgtct tggtaatgag cttacaccga attttacctg tcagtcgccc
     3481 ctcgtggaac ttttgatgac cacagggttt ctcctcgcgt tccaccttct tatggggttc
     3541 gacgaaatag tgtgggtaat atactttctc tgcctcgatg cgaacctcgg catcagtttt
     3601 gggcttttct ggaacggcta catctgtctc atcaaaggca ggattcatga ggcgagaaat
     3661 cctcttatca tctcccttta tacccagact cttgacctgt ccgtagccga tggcgctctt
     3721 tccaccgagg ggataaaggc cattattcac atctgcaaga gccttaccga gtgccttaca
     3781 gtactcttcg tcctcgagaa cgtccctgcg gatccagaag gagcctttca acatgagcgg
     3841 tcttgcaggg ctaccgggca gaggggaatc atcgaatttc ttcttatcag ctgcaccgcc
     3901 agtgaatcta tcgatggcca catgatcgaa ggtcacaggt tccgggtcac tttcgaatac
     3961 gagatcttca aaccttattt tgccggcttc atactcactt ccaaagattt ggcacaggag
     4021 acattcacag tcactgtgag tcaactcagt caaagggacg ccatcttcca tatgaatgcg
     4081 tgccacggca cttctgatca cccctctaaa agattcggcc ttgtatgcac atacgggttt
     4141 cgcctcttcc ccttcagctt tatacttaac gaaggtaacc acatctgtac cacgtttgtc
     4201 cacggctgcg cggattgggt cgccattaat aaaagggctt gccatctcaa tggaaacgtt
     4261 aatctcgtgc cattttggat ccctataatt accgggctct ggcagacctg agttcagtcg
     4321 tttcttcagt tcctcgagcc ccttctcgtc acgccatccc caattcttaa gatagtcatt
     4381 cctttgattc tcgtcgctca gatccagcgt ttcatattta gcattttcca tccggaatcg
     4441 gcccttcccg gttgaggcag cgccactcat gaatgcctgc ccctcagccc accatttcaa
     4501 caccgttttc agtgcatcgg gcagcccatc ttcagagccc cggtaccgga gttgaaatgg
     4561 aaagacaatc ccctcggggg ctacttccat attaaacagg gcaccttccg caaccgtgcc
     4621 tgtgaaggga ttaatgcggg tacgatgcct tatttctggc ggggcattgt actcactgcg
     4681 cgcatccatc accgtgatcc ctctcataat cctacacgtc ttacacatgc atggccgtcc
     4741 acccaactca gcattgcatg ggctatcaaa ataggtttga agatctcgac ggagtatacc
     4801 tcgcacggcg cttcttggca gtctatactt gttatcggga gtgaggagta cttgcaggtc
     4861 ggtttgtttt gcatcttcgt ctatcgctcc gaagaagaag ggcgttttag cgaccagttc
     4921 gccgcatacc acagtttctt tcaacacaga tccgattgac acagaccggc tcttttcaag
     4981 ccgatcgggc tttccatgga attcagcatc gcccagtatt ctccgagtaa ttttcaggcc
     5041 gccactcatg tctttactct tgaggtacag cgcttcaccc agcttctcgc agaattctct
     5101 ccattttccg gcattcttga gttccttggt gtctgctgag gtcgtaagga tttgcctaat
     5161 agtgacagag ttttcgtctt tcttcttctt gccgatatcc cagaggtaat ggtcatcctt
     5221 cccgtcatga tcctttggca agccagcgac aagtttacta gagcggcgga gtgaacgaat
     5281 agcatcagca agcaggcggg tatattcggt tttcttatta tcgtccagaa tgctaataat
     5341 ttgctctgcg gtcttttcag cgagattagc attcggttct gcttggacat tttcggtttg
     5401 tttcccacta tcggctgctg gtgtatattc atcgcggccg cggatctcta gcggatctga
     5461 cggttcacta aaccagctct gcttatatag acctcccacc gtacacgcct accgcccatt
     5521 tgcgtcaatg gggcggagtt gttacgacat tttggaaagt cccgttgatt ttggtgccaa
     5581 aacaaactcc cattgacgtc aatggggtgg agacttggaa atccccgtga gtcaaaccgc
     5641 tatccacgcc cattgatgta ctgccaaaac cgcatcacca tggtaatagc gatgactaat
     5701 acgtagatgt actgccaagt aggaaagtcc cataaggtca tgtactgggc ataatgccag
     5761 gcgggccatt taccgtcatt gacgtcaata gggggcgtac ttggcatatg atacacttga
     5821 tgtactgcca agtgggcagt ttaccgtaaa tactccaccc attgacgtca atggaaagtc
     5881 cctattggcg ttactatggg aacatacgtc attattgacg tcaatgggcg ggggtcgttg
     5941 ggcggtcagc caggcgggcc atttaccgta agttatgtaa cgcggaactc catatatggg
     6001 ctatgaacta atgaccccgt aattgattac tattaataac tagtcaataa tcaatgtcaa
     6061 cgcgtatatc tggcccgtac atcgcgaagc agcgcaaaac gcctaaccct aagcagattc
     6121 ttcatgcaat tgtcggtcaa gccttgcctt gttgtagctt aaattttgct cgcgcactac
     6181 tcagcgacct ccaacacaca agcagggagc agatactggc ttaactatgc ggcatcagag
     6241 cagattgtac tgagagtcga ccctagggga tcgggagatc gatctcccga tccgtcgacg
     6301 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     6361 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     6421 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
     6481 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     6541 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     6601 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
     6661 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
     6721 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
     6781 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
     6841 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
     6901 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
     6961 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
     7021 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
     7081 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
     7141 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
     7201 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
     7261 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
     7321 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     7381 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagt gtcagacccc
     7441 gtagaaaaga tcaaaggatc ttc
//
