LOCUS       sequence-437441-       11740 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Transient expression of GFP-tagged CBPdeltaIDR1 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11740)
  TITLE     Bose Lab CBP Expression Plasmids
REFERENCE   2  (bases 1 to 11740)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pAcGFP-N1_CBPdIDR1
FEATURES             Location/Qualifiers
     source          1..11740
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     misc_feature    609..665
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      5221..5230
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7687..8406
                     /codon_start=1
                     /product="Aequorea coerulescens GFP"
                     /label=AcGFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGAELFTGIVPILIELNGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFEDD
                     GNYKSRAEVKFEGDTLVNRIELTGTDFKEDGNILGNKMEYNYNAHNVYIMTDKAKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMIYF
                     GFVTAAAITHGMDELYK"
     polyA_signal    8528..8649
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8565..8584)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8619..8638
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(8656..9111)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8793..8814)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9005..9024
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        9138..9242
                     /gene="bla"
                     /label=AmpR promoter
     promoter        9244..9601
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        9244..9435
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(9267..9287)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      9452..9587
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9636..10430
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             9636..10430
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(9690..9709)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     10300..10319
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(10618..10637)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    10662..10709
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      11038..11626
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11527..11546
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggactcag atctcgagct caagcttcga attctgcagt cgacggtacc gcgggcccgg
      661 gatccaatgg ctgagaactt gctggacgga ccgcccaacc ccaaaagagc caaactcagc
      721 tcgcccggtt tctcggcgaa tgacagcaca gattttggat cattgtttga cttggaaaat
      781 gatcttcctg atgagctgat acccaatgga ggagaattag gccttttaaa cagtgggaac
      841 cttgttccag atgctgcttc caaacataaa caactgtcgt gcatgaatgc taactttaac
      901 cagacccacc caggcctcct caatagtaac tctggccata gcttaattaa tcaggcttca
      961 caagggcagg cgcaagtcat gaatggatct cttggggctg ctggcagagg aaggggagct
     1021 ggaatgccgt accctactcc agccatgcag ggcgcctcga gcagcgtgct ggctgagacc
     1081 ctaacgcagg tttccccgca aatgactggt cacgcgggac tgaacaccgc acaggcagga
     1141 ggcatggcca agatgggaat aactgggaac acaagtccat ttggacagcc ctttagtcaa
     1201 gctggagggc agccaatggg agccactgga gtgaaccccc agttagccag caaacagagc
     1261 atggtcaaca gtttgcccac cttccctaca gatatcaaga atacttcagt caccaacgtg
     1321 ccaaatatgt ctcagatgca aacatcagtg ggaattgtac ccacacaagc aattgcaaca
     1381 ggccccactg cagatcctga aaaacgcaaa ctgatacagc agcagctggt tctactgctt
     1441 catgctcata agtgtcagag acgagagcaa gcaaacggag aggttcgggc ctgctcgctc
     1501 ccgcattgtc gaaccatgaa aaacgttttg aatcacatga cgcattgtca ggctgggaaa
     1561 gcctgccaag ttgcccattg tgcatcttca cgacaaatca tctctcattg gaagaactgc
     1621 acacgacatg actgtcctgt ttgcctccct ttgaaaaatg ccagtgacaa gcgaaaccaa
     1681 caaaccatcc tggggtctcc agctagtgga attcaaaaca caattggttc tgttggcaca
     1741 gggcaacaga atgccacttc tttaagtaac ccaaatccca tagaccccag ctccatgcag
     1801 cgagcctatg ctgctctcgg actcccctac atgaaccagc cccagacgca gctgcagcct
     1861 caggttcctg gccagcaacc agcacagcct caaacccacc agcagatgag gactctcaac
     1921 cccctgggaa ataatccaat gaacattcca gcaggaggaa taacaacaga tcagcagccc
     1981 ccaaacttga tttcagaatc agctcttccg acttccctgg gggccacaaa cccactgatg
     2041 aacgatggct ccaactctgg taacattgga accctcagca ctataccaac agcagctcct
     2101 ccttctagca ccggtgtaag gaaaggctgg cacgaacatg tcactcagga cctgcggagc
     2161 catctagtgc ataaactcgt ccaagccatc ttcccaacac ctgatcccgc agctctaaag
     2221 gatcgccgca tggaaaacct ggtagcctat gctaagaaag tggaagggga catgtacgag
     2281 tctgccaaca gcagggatga atattatcac ttattagcag agaaaatcta caagatacaa
     2341 aaagaactag aagaaaaacg gaggtcgcgt ttacataaac aaggcatctt ggggaaccag
     2401 ccagccttac cagccccggg ggctcagccc cctgtgattc cacaggcaca acctgtgaga
     2461 cctccaaatg gacccctgtc cctgccagtg aatcgcatgc aagtttctca agggatgaat
     2521 tcatttaacc ccatgtcctt ggggaacgtc cagttgccac aagcacccat gggacctcgt
     2581 gcagcctccc caatgaacca ctctgtccag atgaacagca tgggctcagt gccagggatg
     2641 gccatttctc cttcccgaat gcctcagcct ccgaacatga tgggtgcaca caccaacaac
     2701 atgatggccc aggcgcccgc tcagagccag tttctgccac agaaccagtt cccgtcatcc
     2761 agcggggcga tgagtgtggg catggggcag ccgccagccc aaacaggcgt gtcacaggga
     2821 caggtgcctg gtgctgctct tcctaaccct ctcaacatgc tggggcctca ggccagccag
     2881 ctaccttgcc ctccagtgac acagtcacca ctgcacccaa caccgcctcc tgcttccacg
     2941 gctgctggca tgccatctct ccagcacacg acaccacctg ggatgactcc tccccagcca
     3001 gcagctccca ctcagccatc aactcctgtg tcgtcttccg ggcagactcc caccccgact
     3061 cctggctcag tgcccagtgc tacccaaacc cagagcaccc ctacagtcca ggcagcagcc
     3121 caggcccagg tgaccccgca gcctcaaacc ccagttcagc ccccgtctgt ggctacccct
     3181 cagtcatcgc agcaacagcc gacgcctgtg cacgcccagc ctcctggcac accgctttcc
     3241 caggcagcag ccagcattga taacagagtc cctaccccct cctcggtggc cagcgcagaa
     3301 accaattccc agcagccagg acctgacgta cctgtgctgg aaatgaagac ggagacccaa
     3361 gcagaggaca ctgagcccga tcctggtgaa tccaaagggg agcccaggtc tgagatgatg
     3421 gaggaggatt tgcaaggagc ttcccaagtt aaagaagaaa cagacatagc agagcagaaa
     3481 tcagaaccaa tggaagtgga tgaaaagaaa cctgaagtga aagtagaagt taaagaggaa
     3541 gaagagagta gcagtaacgg cacagcctct cagtcaacat ctccttcgca gccgcgcaaa
     3601 aaaatcttta aaccagagga gttacgccag gccctcatgc caaccctaga agcactgtat
     3661 cgacaggacc cagagtcatt acctttccgg cagcctgtag atccccagct cctcggaatt
     3721 ccagactatt ttgacatcgt aaagaatccc atggacctct ccaccatcaa gcggaagctg
     3781 gacacagggc aataccaaga gccctggcag tacgtggacg acgtctggct catgttcaac
     3841 aatgcctggc tctataatcg caagacatcc cgagtctata agttttgcag taagcttgca
     3901 gaggtctttg agcaggaaat tgaccctgtc atgcagtccc ttggatattg ctgtggacgc
     3961 aagtatgagt tttccccaca gactttgtgc tgctatggga agcagctgtg taccattcct
     4021 cgcgatgctg cctactacag ctatcagaat aggtatcatt tctgtgagaa gtgtttcaca
     4081 gagatccagg gcgagaatgt gaccctgggt gacgaccctt cacagcccca gacgacaatt
     4141 tcaaaggatc agtttgaaaa gaagaaaaat gataccttag accccgaacc tttcgttgat
     4201 tgcaaggagt gtggccggaa gatgcatcag atttgcgttc tgcactatga catcatttgg
     4261 ccttcaggtt ttgtgtgcga caactgcttg aagaaaactg gcagacctcg aaaagaaaac
     4321 aaattcagtg ctaagaggct gcagaccaca agactgggaa accacttgga agaccgagtg
     4381 aacaaatttt tgcggcgcca gaatcaccct gaagccgggg aggtttttgt ccgagtggtg
     4441 gccagctcag acaagacggt ggaggtcaag cccgggatga agtcacggtt tgtggattct
     4501 ggggaaatgt ctgaatcttt cccatatcga accaaagctc tgtttgcttt tgaggaaatt
     4561 gacggcgtgg atgtctgctt ttttggaatg cacgtccaag aatacggctc tgattgcccc
     4621 cctccaaaca cgaggcgtgt gtacatttct tatctggata gtattcattt cttccggcca
     4681 cgttgcctcc gcacagccgt ttaccatgag atccttattg gatatttaga gtatgtgaag
     4741 aaattagggt atgtgacagg gcacatctgg gcctgtcctc caagtgaagg agatgattac
     4801 atcttccatt gccacccacc tgatcaaaaa atacccaagc caaaacgact gcaggagtgg
     4861 tacaaaaaga tgctggacaa ggcgtttgca gagcggatca tccatgacta caaggatatt
     4921 ttcaaacaag caactgaaga caggctcacc agtgccaagg aactgcccta ttttgaaggt
     4981 gatttctggc ccaatgtgtt agaagagagc attaaggaac tagaacaaga agaagaggag
     5041 aggaaaaagg aagagagcac tgcagccagt gaaaccactg agggcagtca gggcgacagc
     5101 aagaatgcca agaagaagaa caacaagaaa accaacaaga acaaaagcag catcagccgc
     5161 gccaacaaga agaagcccag catgcccaac gtgtccaatg acctgtccca gaagctgtat
     5221 gccaccatgg agaagcacaa ggaggtcttc ttcgtgatcc acctgcacgc tgggcctgtc
     5281 atcaacaccc tgccccccat cgtcgacccc gaccccctgc tcagctgtga cctcatggat
     5341 gggcgcgacg ccttcctcac cctcgccaga gacaagcact gggagttctc ctccttgcgc
     5401 cgctccaagt ggtccacgct ctgcatgctg gtggagctgc acacccaggg ccaggaccgc
     5461 tttgtctaca cctgcaacga gtgcaagcac cacgtggaga cgcgctggca ctgcactgtg
     5521 tgcgaggact acgacctctg catcaactgc tataacacga agagccatgc ccataagatg
     5581 gtgaagtggg ggctgggcct ggatgacgag ggcagcagcc agggcgagcc acagtcaaag
     5641 agcccccagg agtcacgccg gctgagcatc cagcgctgca tccagtcgct ggtgcacgcg
     5701 tgccagtgcc gcaacgccaa ctgctcgctg ccatcctgcc agaagatgaa gcgggtggtg
     5761 cagcacacca agggctgcaa acgcaagacc aacgggggct gcccggtgtg caagcagctc
     5821 atcgccctct gctgctacca cgccaagcac tgccaagaaa acaaatgccc cgtgcccttc
     5881 tgcctcaaca tcaaacacaa gctccgccag cagcagatcc agcaccgcct gcagcaggcc
     5941 cagctcatgc gccggcggat ggccaccatg aacacccgca acgtgcctca gcagagtctg
     6001 ccttctccta cctcagcacc gcccgggacc cccacacagc agcccagcac accccagacg
     6061 ccgcagcccc ctgcccagcc ccaaccctca cccgtgagca tgtcaccagc tggcttcccc
     6121 agcgtggccc ggactcagcc ccccaccacg gtgtccacag ggaagcctac cagccaggtg
     6181 ccggcccccc cacccccggc ccagccccct cctgcagcgg tggaagcggc tcggcagatc
     6241 gagcgtgagg cccagcagca gcagcacctg taccgggtga acatcaacaa cagcatgccc
     6301 ccaggacgca cgggcatggg gaccccgggg agccagatgg cccccgtgag cctgaatgtg
     6361 ccccgaccca accaggtgag cgggcccgtc atgcccagca tgcctcccgg gcagtggcag
     6421 caggcgcccc ttccccagca gcagcccatg ccaggcttgc ccaggcctgt gatatccatg
     6481 caggcccagg cggccgtggc tgggccccgg atgcccagcg tgcagccacc caggagcatc
     6541 tcacccagcg ctctgcaaga cctgctgcgg accctgaagt cgcccagctc ccctcagcag
     6601 caacagcagg tgctgaacat tctcaaatca aacccgcagc taatggcagc tttcatcaaa
     6661 cagcgcacag ccaagtacgt ggccaatcag cccggcatgc agccccagcc tggcctccag
     6721 tcccagcccg gcatgcaacc ccagcctggc atgcaccagc agcccagcct gcagaacctg
     6781 aatgccatgc aggctggcgt gccgcggccc ggtgtgcctc cacagcagca ggcgatggga
     6841 ggcctgaacc cccagggcca ggccttgaac atcatgaacc caggacacaa ccccaacatg
     6901 gcgagtatga atccacagta ccgagaaatg ttacggaggc agctgctgca gcagcagcag
     6961 caacagcagc agcaacaaca gcagcaacag cagcagcagc aagggagtgc cggcatggct
     7021 gggggcatgg cggggcacgg ccagttccag cagcctcaag gacccggagg ctacccaccg
     7081 gccatgcagc agcagcagcg catgcagcag catctccccc tccagggcag ctccatgggc
     7141 cagatggcgg ctcagatggg acagcttggc cagatggggc agccggggct gggggcagac
     7201 agcaccccca acatccagca agccctgcag cagcggattc tgcagcaaca gcagatgaag
     7261 cagcagattg ggtccccagg ccagccgaac cccatgagcc cccagcaaca catgctctca
     7321 ggacagccac aggcctcgca tctccctggc cagcagatcg ccacgtccct tagtaaccag
     7381 gtgcggtctc cagcccctgt ccagtctcca cggccccagt cccagcctcc acattccagc
     7441 ccgtcaccac ggatacagcc ccagccttcg ccacaccacg tctcacccca gactggttcc
     7501 ccccaccccg gactcgcagt caccatggcc agctccatag atcagggaca cttggggaac
     7561 cccgaacaga gtgcaatgct cccccagctg aacaccccca gcaggagtgc gctgtccagc
     7621 gaactgtccc tggtcgggga caccacgggg gacacgctag agaagtttgt ggagggcttg
     7681 ccggtcatgg tgagcaaggg cgccgagctg ttcaccggca tcgtgcccat cctgatcgag
     7741 ctgaatggcg atgtgaatgg ccacaagttc agcgtgagcg gcgagggcga gggcgatgcc
     7801 acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc tgtgccctgg
     7861 cccaccctgg tgaccaccct gagctacggc gtgcagtgct tctcacgcta ccccgatcac
     7921 atgaagcagc acgacttctt caagagcgcc atgcctgagg gctacatcca ggagcgcacc
     7981 atcttcttcg aggatgacgg caactacaag tcgcgcgccg aggtgaagtt cgagggcgat
     8041 accctggtga atcgcatcga gctgaccggc accgatttca aggaggatgg caacatcctg
     8101 ggcaataaga tggagtacaa ctacaacgcc cacaatgtgt acatcatgac cgacaaggcc
     8161 aagaatggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggatgg cagcgtgcag
     8221 ctggccgacc actaccagca gaataccccc atcggcgatg gccctgtgct gctgcccgat
     8281 aaccactacc tgtccaccca gagcgccctg tccaaggacc ccaacgagaa gcgcgatcac
     8341 atgatctact tcggcttcgt gaccgccgcc gccatcaccc acggcatgga tgagctgtac
     8401 aagtgagcgg ccgcgactct agatcataat cagccatacc acatttgtag aggttttact
     8461 tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt
     8521 tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     8581 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     8641 tgtatcttaa ggcgtaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt
     8701 gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa
     8761 aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa
     8821 agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac
     8881 gtgaaccatc accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga
     8941 accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa
     9001 aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc
     9061 tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg tcaggtggca
     9121 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     9181 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     9241 gtcctgaggc ggaaagaacc agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc
     9301 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg
     9361 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     9421 agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
     9481 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
     9541 ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa
     9601 agatcgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg
     9661 caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa
     9721 tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg
     9781 tcaagaccga cctgtccggt gccctgaatg aactgcaaga cgaggcagcg cggctatcgt
     9841 ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa
     9901 gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc
     9961 ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg
    10021 ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg
    10081 aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg
    10141 aactgttcgc caggctcaag gcgagcatgc ccgacggcga ggatctcgtc gtgacccatg
    10201 gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact
    10261 gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg
    10321 ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc
    10381 ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct
    10441 ggggttcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt tcgattccac
    10501 cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat
    10561 cctccagcgc ggggatctca tgctggagtt cttcgcccac cctaggggga ggctaactga
    10621 aacacggaag gagacaatac cggaaggaac ccgcgctatg acggcaataa aaagacagaa
    10681 taaaacgcac ggtgttgggt cgtttgttca taaacgcggg gttcggtccc agggctggca
    10741 ctctgtcgat accccaccga gaccccattg gggccaatac gcccgcgttt cttccttttc
    10801 cccaccccac cccccaagtt cgggtgaagg cccagggctc gcagccaacg tcggggcggc
    10861 aggccctgcc atagcctcag gttactcata tatactttag attgatttaa aacttcattt
    10921 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
    10981 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
    11041 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
    11101 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
    11161 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
    11221 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
    11281 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
    11341 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
    11401 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
    11461 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
    11521 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
    11581 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
    11641 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
    11701 atcccctgat tctgtggata accgtattac cgccatgcat
//
